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Majalah Obat Tradisional
Published by Universitas Gadjah Mada
ISSN : 14105918     EISSN : 24069086     DOI : -
Core Subject : Health,
Health Professions Medicine & Pharmacology Public Health
raditional Medicine Journal (Majalah Obat Tradisional), or Trad. Med. J. (ISSN 1410-5918 (print) and ISSN 2406-9086 (online)), is an international scientific journal published by Faculty of Pharmacy, Universitas Gadjah Mada, three times annually. Collaborating with Indonesian Pharmacist Association, Daerah Istimewa Yogyakarta, and we dedicate our journal to researches and development in traditional medicine. The journal receives papers on research laboratory, field research, and case studies of traditional medicine and its constituent, covering research topics including raw materials, cultivation, phytochemical, pharmacological effects and toxicology, formulation, and biotechnology.
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Articles 10 Documents
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Search results for , issue "Vol 24, No 2 (2019)" : 10 Documents clear
The Effect of Extraction Method on Total Phenolic Content and Antioxidant Activity of Salam Leaves (Syzygium polyanthum) using DPPH (1,1-Diphenyl-2-Picrylhidrazil) Sri Luliana; Hafrizal Riza; Eneng Neni Indriyani
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.33955

Abstract

Extraction is an important step in separating bioactive compounds from the plant. The selection of extraction technique is also important in the standardization of herbal products, for if not observed it can remove the desired soluble constituents. One of Indonesia’s endemic plants which have been proven to have an antioxidant activity is Salam leaves (Syzygium polyanthum). This study aims to determine the effect of extraction method on total phenolic content and antioxidant activity of S. polyanthum leaves by counteracting free radicals mechanism using DPPH. The S. polyanthum leaves were extracted by maceration, soxletation, and infusa methods, and tested for the total phenolic content and the antioxidant activity. The results showed that the total phenolic content of each method extration is different. The total of the phenolic content extraction by maceration, soxhlet, and infusa method respectively were 338.62±21.3; 227.72±21.6; and 144.48±8.2 mgGAE/g. The best antioxidant activity was maceration method with IC50 17.53±0.11 µg/mL followed by soxhlet and infusa which were 18.73±0.31 and 40.26±0.18 µg/mL. The research conclusion is that the extraction method has an effect on the total phenol and antioxidant activity of S. polyanthum leaves.
The Effect of Dayak Onion Bulb Ethanol Extract (Sisyrinchium palmifolium L.) on Triglyceride Level and Aorta Histopathology in Diabetes Melitus White Rat Induced by Alloxan I Gusti Agung Ayu Kusuma Wardani; Fitria Megawati; Ni Made Dharma Shantini Suena
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.481 KB) | DOI: 10.22146/mot.38051

Abstract

The high prevalence of diabetes mellitus in Indonesia has made antidiabetic treatment efforts increasingly popular. In addition, the many side effects caused by the use of chemical drugs, triggered the development of research on herbal therapy. The purpose of this study was to determine the effectiveness of Dayak onion bulb extract in reducing two-hour postprandial blood glucose, triglyceride levels, and the amount of lipid vacuoles in aorta blood vessels of diabetic rat. The long-term goal of this study is the creation of innovation products in the form of standardized herbs as complementary therapies that can be used daily by the community, so that the risk of morbidity can be reduced. The research design used was randomized control group pretest posttest design. The results of blood glucose analysis showed that there were significant differences between the positive control group with the 400 mg/KgBW and 800 mg/KgBW extract group, with a significance value of 0,000. In the extract group, 400 mg/KgBW with 800 mg/KgBW extract group showed no significant difference with a significance value of 0.390. The results of the analysis of triglyceride levels showed no significant difference between the positive control group with the 400 mg/KgBW extract group, with a significance value of 0.981. However, there were significant differences between the positive control group with the 800 mg/KgBW extract group with a significance value of 0.025. Between the 400 mg/KgBW and 800 mg/KgBW extract groups, there was a difference with a significance value of 0.024. Aorta histopathology results showed that there was a significant difference in the number of lipid vacuoles between the positive control group and the 400 mg/KgBW extract group and 800 mg/KgBW extract group. Based on the research, it can be concluded that the extract dose of 400 mg/KgBW is a dose that is able to influence the decrease in blood glucose levels, triglycerides and the amount of lipid vacuoles in the aorta blood vessels.
Combination Effects of Moringa oleifera Leaf Ethanol Extract and Andrographis paniculata Herb on Blood Glucose Levels and Pancreas Histopathology of Diabetic Rats Induced by Streptozotocin Annisa Fatmawati; Moch. Saiful Bachri; Laela Hayu Nurani
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2639.634 KB) | DOI: 10.22146/mot.39401

Abstract

Type 2 diabetes mellitus is a non-contagious disease, can damage the glucose metabolic system in the body, and is characterized by hyperglycemic conditions. Moringa leaves (Moringa oleifera) and Andrographis herbs (Andrographis paniculata) have been studied to have antihyperglycemic activity in diabetic rats. The purpose of this study was to determine the effect of the combination of Moringa leaf ethanol extract (MLEE) and ethanol extract of Andrographis herbs (AHEE) in streptozotocin-induced rats (45 mg/kgBW). The subjects consisted of 32 rats, divided into 8 groups (1 healthy group and 7 type 2 DM groups). The study was conducted for 4 weeks by measuring blood glucose levels in pre-treatment, day 0, 14 and 28 with a single MLEE treatment dose of 300 mg/kgBW, single AHEE 300 mg/kgBW, combination of MLEE and AHEE 150+150 mg/kgBW, 200+100 mg/kgBW, 100 + 200 mg/kgBW, and gliclazide 5 mg/kgBW orally. The results of measurement of fasting blood glucose levels on day 28 showed that administration of gliclazide 5 mg/kg BW, single dose MLEE and AHEE, as well as its combination, had significant differences (p<0.05) compared to the hyperglycemic control group. Pancreatic organ histopathology features in the extract dose group showed that there was a change in the repair of insula Langerhans compared to the hyperglycemic control group which showed necrotic damage due to streptozotocin induction. Combination administration has the same antihyperglycemic effect by single dose extract in diabetic rats within 28 days, which also restore weight loss to normal.
Antioxidant Activities of n-Hexane Soluble and Insoluble Fraction, Ethyl Acetate Soluble and Insoluble Fraction from Ethanol Extract of Sambung Nyawa Leaf (Gynura procumbens (Lour.) Merr.) Purwantiningsih Purwantiningsih; Retno Murwanti; Lukman Hakim
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.43743

Abstract

There were many plants containing flavonoids which can function as antioxidant. Antioxidant can be used to decrease the mortality rate caused by degenerative and infectious diseases. This study aims to determine the antioxidant potency of n-hexane soluble and insoluble fraction, ethyl acetate soluble and insoluble fraction, and the ethanol extract antioxidant activity of Sambung Nyawa leaf (Gynura procumbens (Lour.) Merr.). The ethanol extract of G. procumbens leaves was obtained by maceration using 70% ethanol then fractionated using hexane and ethyl acetate to obtain the n-hexane soluble fraction, n-hexane insoluble fraction, ethyl acetate soluble fraction, and ethyl acetate insoluble fraction. The antioxidant activity study of ethanol extract, n-hexane soluble, and insoluble fraction, ethyl acetate soluble, and insoluble fraction of G. procumbens was carried out using DPPH (2,2- diphenyl-1-picrylhydrazyl) method to evaluate its free radical scavenging activity with a comparison of vitamin C. The antioxidants activity was expressed as IC50 value. The IC50 value for vitamin C and ethanol extract of G. procumbens leaves were 2.41 µg/ml and 200 µg/ml. On the other hand, the n-hexane soluble and insoluble fraction had an IC50 value of 78 µg/ml and 34 µg/ml, while ethyl acetate soluble and insoluble fraction had the potency as an antioxidant with an IC50 value of 419 µg/ml and 151 μg/ml, respectively. Based on the results obtained, the antioxidant potency of G. procumbens ethanol extract and their fraction were successively of n-hexane insoluble fraction > n-hexane soluble fraction > ethyl acetate insoluble fraction > ethanol extract > ethyl acetate soluble fraction.
Determination of Total Xanthone Content in the Preparation of Mangosteen Pericarp Capsules (garcinia mangostana l.) Available on the Market using UV-Visible Spectrophotometry Method Delladari Mayefis; Yandri Anugerah; Roslinda Rasyid
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.43871

Abstract

The skin of Mangosteen fruit (Garcinia mangostana L.) is widely used as traditional medicine, for it contains a lot of xanthone compounds. Today, there are many products from mangosteen pericarp extract being sold on the market, one of which is the preparation of mangosteen pericarp capsules. Determining the total xanthone content in the preparation of mangosteen pericarp capsules (Garcinia mangostana L.) circulating on the market needs to be done to calculate the levels of xanthone that are not included in the preparation label. The results of the analysis with UV-Visible Spectrophotometry obtains the maximum absorption wavelength at 243 nm and the linear regression equation of the calibration curve that is y=0,0568+0,0727x with a value of r=0,9999. Validation of the analysis method showed the results of intraday precision obtained % RSD 0,34%, 0,17%, and 0,16%, interday precision of 0,23%, 0,35%, and 0,16%. The accuracy obtained was mean % recovery 95,55%, limit of detection 0,35 μg/ml and limit of quantitation 1,15 μg/ml. The results of the determination of the total xanthone content in the mangosteen pericarp capsules were 100,8 μg/mg in sample A; 197 μg/mg in sample B; and 50,2 μg/mg in sample C.
Hair Growth Promoting Activity of Green Tea Leaves (Camellia sinensis l.) Ethanolic Extract Vini Noviani; Shelly Thauresia; Elsa Fitria Apriani; Partomuan Simanjuntak
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.44053

Abstract

Hair loss and baldness is one of the abnormalities in the hair that often occurs in both men and women. Many cosmetic products from natural to synthetic materials have been developed to overcome these problems, yet, synthetics product is potential to give side effects, such as local irritation. In this study, green tea (Camellia sinensis L.) leaves were used as active substances. The aim of this study was to attest the hair growth promoting activity of n-hexane, water, and ethyl acetate fractions from ethanolic extract of green tea leaves. Green tea leaves contain flavonoids which can help promoting hair growth. To obtain the compound, green tea leaves were made into ethanolic extract. The extract was obtained by maceration using 70% ethanol then partitioned using n-hexane, ethyl acetate, and water, to obtain the n-hexane, ethyl acetate, and water fractions. Ethyl acetate and water fractions have been shown to contain flavonoids, so it could continue the hair growth promoting activities with concentrations of 1% and 4%. Hair growth promoting activity was performed on rabbits. The results showed that 4% of water fraction containing flavonoids had the best hair growth promoting activity.
The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans Hasyrul Hamzah; Triana Hertiani; Sylvia Utami Tunjung Pratiwi; Titik Nuryastuti
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.442 KB) | DOI: 10.22146/mot.44532

Abstract

Biofilm acts as the mediator for infection nowadays. Approximately, more than 80% infection incidents are biofilm-formation related. Biofilm as bacteria's defense system is more difficult to eradicate by antibiotic; therefore, pathogen bacteria on their biofilm forms can make serious problems for human health. The invention of a new candidate for polymicrobial biofilm can be an essential challenge to be studied, in order to prevent infections related to biofilm. Tannin is a polyphenol compound with anti-bacterial and anti-fungal potential. This study aims to acknowledge the effectiveness of tannin in inhibition and degradation of C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilm. The assay for biofilm inhibition and degradation were determined with microtiter broth method. The effectivity of tannin antibiofilm against polymicrobial biofilm were analyzed by calculating minimum biofilm inhibitory concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The mechanism of action of tannin against polymicrobial biofilm was tested using scanning electron microscopy (SEM). The data were analyzed using the Statistical Package for the Social Sciences (SPSS) with a 95% confidence level. Tannin 1% gave inhibition activity of mono-species biofilm formation S. aureus in the middle phase and maturation of 79.04±0.01, 61.48±0.03, E. coli 74.56±0.01, 67.91±0.02, P. aeruginosa 67.32±0.05, 35.13± 0.01, C. albicans 60.62±0.01, 47.16±0.01. The results also provide evidence that tannin activity can degrade and damage the matrix of extracellular polymeric substance (EPS) polymicrobial biofilms. Hence, tannins can be a potential candidate for new antibiofilm for polymicrobial biofilm.
Study of Impacts of Ganoderma applanatum (Pres.) Pat. Extract on Hepatic and Renal Biochemical Parameters of Rats Sukumar Dandapat; Manoj Kumar; Rakesh Ranjan; Manoranjan Prasad Sinaha
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (20.994 KB) | DOI: 10.22146/mot.44586

Abstract

Traditionally, Ganoderma applanatum was used as medicinal supplement for treatment of different diseases. In this study, biochemical screening, acute toxicity, and impact of G. applanatum aqueous extract on liver and renal parameters were studied. Qualitative screening of G. applanatum aqueous extract showed presence of various biochemicals such as tannin, phenolics, proteins, flavanoids and other biochemicals. FTIR analysis also showed spectrum transmission peaks for different mycochemicals such as3248. 13cm-1 for phenol O-H stretch, 1597.06cm-1 for primary amine N-H stretch, 783.10cm-1 for aromatic (meta disub benzene) C-H stretch. The extract increased body weight (Initial body weight: 181.74g; final body weight: 185.08g) when compared to control group (Initial body weight: 178.61 g; final body weight: 181.14 g) in acute toxicity test dose (2000mg/kg). Similar insignificant in final body weight of animals of different groups were observed when compared to initial body weight of animals used for the study of live and renal profile. The extract had no significant effect on organ weight. Low dose (200mg kg-1) of extract insignificantly decrease AST (51.71±0.61 mg dL-1), ALT (146.07±0.89 mg dL-1), ALP (174.68±0.65 mg dL-1) and bilirubin (0.61±0.01 mg dL-1) level and significantly elevated serum albumin level (6.63±0.22 g dL-1) compare to control group.  Low dose (200mg kg-1) extract showed similar insignificant decrease in serum urea (61.30±1.05 mg dL-1) and creatinine level (0.90±0.02 mg dL-1) and significantly decreased serum uric acid level (19.52±1.14 mg dL-1) compare to control group of rats. Thus, dose-oriented application of G. applanatum extract can be beneficial for treatment of hepatic and renal diseases.
The Effect of Pasak Bumi (Eurycoma longifolia, Jack) Roots Ethanol Extract against Hematology Profile of Healthy Volunteers Laela Hayu Nurani; Fara Azzahra; Sitarina Widyarini; Abdul Rohman
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45533

Abstract

The roots of Eurycoma longifolia, Jack has a lot of beneficial activity on human health. The use of Eurycoma longifolia, Jack roots as a traditional medicine should be made in the form of a dosage that is more effective, safe, and had no side effects, especially on hematology. Easy preparations are capsule. This study aimed at identifying the effect of ethanol extract capsule of Eurycoma longifolia, Jack roots on hematology of healthy volunteers. Healthy volunteers were assigned in the study, each of which were 10 male and 10 female who met the inclusion criteria. Both groups were given ethanol extract capsule of Eurycoma longifolia, Jack roots for 14 days, with the dose of 300 mg extract once a day, after meal, at night. Hematology examination was performed on day 0; 14 and 42. Results were compared statistically using Repeated ANOVA and Friedman tests. The result of the study indicated that the average value of hematology at the examination day of 0; 14; and 42, there were significant differences in MCV and leukocyte parameters in healthy male volunteers (p<0.05). MCH, LED and monocyte parameters in healthy female volunteers had a significant difference of (p<0.05). However, the parameter values were still within the normal range. Monitoring results showed that some of the side effects were frequent urinary, dizziness, nausea, sweating, insomnia, and increasing appetite on healthy male volunteers. Meanwhile, on healthy female volunteers, there were frequent urinary, insomnia, constipation. Having ethanol extract capsule of the Eurycoma longifolia, Jack roots did not affect the hematology parameters of healthy male and female volunteers, but it caused some side effects. 
Prediction of Geraniol Bond Mode in Aspergillus niger Linalool Dehydratase – Isomerase Yelfi Anwar; Andrianopsyah Mas Jaya Putra; Elvina Dhiaul Iftitah; Partomuan Simanjuntak; Shirly Kumala
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.33 KB) | DOI: 10.22146/mot.45599

Abstract

Geraniol is a very valuable aroma chemical and has commonly been used in fragrances and aroma compound. Geraniol biotransformation by Aspergillus niger has been studied. The main bioconversion products obtained from geraniol and liquid culture of A. niger are linalool and alpha-terpineol. Linalool plays a major role in anti-inflammatory, antibacterial and antioxidant activities. This study aims to know the interaction of geraniol in Aspergillus niger enzyme with docking molecular. Comparative modeling of Aspergillus niger enzyme was conducted by means of one of the crystal structure of Linalool Dehydratase – Idomerase (LDI) as a template. The best model of this comparative modeling was then used in docking molecular to investigate geraniol binding mode inactive site enzyme of Aspergillus niger. Inactive site enzyme of Aspergillus niger, geraniol is located with hydrophobic and hydrogen bonds: Amino acid – the amino acids are Asn 105, Arg 96, Lys 112 inactive site - OH with hydrogen bond, Arg 97 inactive site – CH3 with hydrophobic bond and Leu54 inactive site – CH3 with the hydrophobic bond. The distances among pharmacophore respectively are 3,603 A, 6,768 A, and 7,345A. It has higher score (ΔGbind: -3.4 kcal/mol) compared to linalool (ΔGbind: -3.6 kcal/mol). Virtual tethering of linalool with LDI Aspergillus niger enzyme in amino acid Leu120 and Glu118 had been done. The pharmacophore is - OH and methyl C8 group. The distances among pharmacophore respectively are 5,835 Å, 2,52 Å, and 5,32 Å. Virtual tethering of LDI Aspergillus niger enzyme with geraniol has a higher score (ΔGbind: -3.4 kcal/mol) compared to linalool (ΔGbind: -3.6 kcal/mol). It shows that interaction between linalool and LDI Aspergillus niger enzyme is easier to occur than the interaction between geraniol and LDI Aspergillus niger enzyme, geraniol reaction to linalool that occurs is rearrangement reaction.

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