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All Journal Indonesian Journal of Cancer Chemoprevention Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Majalah Farmaseutik JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Ilmu Kefarmasian Indonesia Borneo Journal of Pharmacy

Sylvia Utami Tunjung Pratiwi

Faculty Of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia

Author-ID : 346441

Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Efficacy of Thymol and Eugenol Against Polymicrobial Biofilm Hamzah, Hasyrul; Tunjung Pratiwi, Sylvia Utami; Hertiani, Triana
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Biofilms associated with human infection have high levels of pathogenicity due to their resistance to antibiotics. The discovery of an active antibiofilm agent against polymicrobial biofilms is a necessary consequence for coping with biofilm-related infections. Thymol and Eugenol are essential oils that have potential as antibacterial and antifungal. This study aimed to determine the effectiveness of thymol and eugenol inhibits C. albicans, P. Aeruginosa, E. coli S. aureus and polymicrobial biofilm. Biofilm formation inhibition assay and biofilm degradation assay of thymol and eugenol were determined using microtiter broth method.The antibiofilm efficacy of thymol and eugenol towards polymicrobial biofilms were analyzed by calculating minimum biofilm inhibitor concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The data were analyzed using Statistical Package for the Social Sciences (SPSS) with 95% confidence level. Thymol and eugenol showed inhibitory activity against the formation of mono and polymicrobial biofilms of the microbial tested.The result also demonstrated an evidence of activity of thymol and eugenol in breaking down mono and polymicrobial biofilm. Therefore, thymol and eugenol serves as a potential source for new antibiofilm drugs towards polymicrobial biofilm.

Effect of cengkeh leaves and kayu manis cortex essential oils blend as anti dental plaque Ardani, Marisya; Pratiwi, Sylvia Utami Tunjung; Hertiani, Triana
Indonesian Journal of Pharmacy Vol 21 No 3, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Dental plaque is a mouth cavity health problem related to microbial biofilm, where Streptococcus mutans is predominant. Adding of essential oils blend in mouthwash has been reported to increase the dental plaque inhibitory activity. The essential oils of clove leaves (Syzygium aromaticum (L.) Merr. & Perry) and cinnamon cortex (Cinnamomum burmanniNees ex Bl.), are known as potential antibacterial and antibiofilm towards S. mutans. This research aims were to reveal the influence of blending the clove leaves and cinnamon cortex essential oils in antibacterial and anti biofilm activity against S. mutans and to find out the optimum composition. Antibacterial assay was performed in nutrient broth media, on microplate flat-bottom polystyrene 96 wells. Biofilm formation inhibition and degradation assays were done in BHI + 2 % sucrose on microplate flexible U-bottom PVC 96 wells. Crystal violet1 % was used to stain the biofilm and Optical Density(OD) was measured at λ 595 nm. Simplex Lattice Design formula was used to calculate the blend optimum composition. TLCbioautography and GC-MS assays were done to revealthe active substances. As conclusion, it was proven that blending the clove leaves and cinnamon cortex essential oils increased the antibacterial and biofilm degradation potency, but reduced the biofilm formation inhibitory effect against S. mutans. The optimum composition of the essential oils blend was 27:73 (% v/v). From our results we suggest that the clove leaves and cinnamon cortex essential oil blend used in this study be developed as anti dental plaque.Key words : clove leaves, cinnamon cortex, essential oils blend, Streptococcus mutans

FORMULASI MOUTHWASH NANOEMULSI KOMBINASI MINYAK SEREH (Cymbopogon citratus) DAN MINYAK JERUK PURUT (Citrus hystrix) SEBAGAI ANTIKANDIDIASIS ORAL TERUJI IN VITRO TERHADAP Candida albanicans ATCC 10231 Meta Juniatik; Khoirunnissa Hida; Fransisca Priskaningtyas Wulandari; Nurul Pangestuti; Ni'matul Munawaroh; Ronny Martien; Sylvia Utami Tunjung Pratiwi
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Kandidiasis adalah penyakit infeksi jamur yang masih menjadi masalah kesehatan di Indonesia, kandidiasis terutama oral sebagian besar dikarenakan pertumbuhan abnormal Candida albicans pada rongga mulut. Minyak jeruk purut dan serai telah terbukti memiliki aktivitas antijamur terhadap Candida albicans. Komposisi limonen, α-terpineol, sitronelil assetat, terpineol, β-pinene, pada minyak jeruk purut dan citronelal pada minyak sereh membuat kombinasi keduanya minyak memiliki potensi sebagai terapi alternatif untuk kandidiasis oral. Kedua minyak ini dapat diformulasikan dalam bentuk nanoemulsion obat kumur. Nanoemulsion dipilih karena memiliki beberapa keuntungan seperti yang jelas, stabil, dan meningkatkan aktivitas antijamur minyak sereh dan minyak jeruk purut. Penelitian ini dimulai dengan determinasi tanaman, uji karakterisasi minyak atsiri (kelarutan, indeks bias, dan berat jenis), dan analisis kandungan minyak atsiri dengan GC-MS. Nanoemulision diformulasikan dengan metode titrasi air. Optimalisasi campuran minyak dan VCO dilakukan untuk mendapatkan nanoemulsion yang jernih dan stabil. Formula terbaik dikarakterisasi (ukuran partikel, viskositas, potensi zeta, dan stabilitasnya), dilakukan analisis senyawa kimia minyak atsiril dalam formula optimum dan menguji efek penghambatan formula oleh metode mikrodilusi. Analisis data daya hambat dilakukan dengan One Way ANOVA. Berdasarkan hasil penelitian, 0,4% minyak campuran, 3,6% VCO, 17,3% tween 80, 8,7% PEG 400 dan 70% air merupakan formula terbaik yang dapat membentuk sistem nanoemulsion dengan rata-rata ukuran partikel 21,4 nm, viskositas rendah, potensi zeta yang rendah dan stabil selama penyimpanan freez-thaw. Nanoemulsi secara signifikan memiliki efek penghambatan lebih efektif daripada kontrol positif.

The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans Hasyrul Hamzah; Triana Hertiani; Sylvia Utami Tunjung Pratiwi; Titik Nuryastuti
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Biofilm acts as the mediator for infection nowadays. Approximately, more than 80% infection incidents are biofilm-formation related. Biofilm as bacteria's defense system is more difficult to eradicate by antibiotic; therefore, pathogen bacteria on their biofilm forms can make serious problems for human health. The invention of a new candidate for polymicrobial biofilm can be an essential challenge to be studied, in order to prevent infections related to biofilm. Tannin is a polyphenol compound with anti-bacterial and anti-fungal potential. This study aims to acknowledge the effectiveness of tannin in inhibition and degradation of C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilm. The assay for biofilm inhibition and degradation were determined with microtiter broth method. The effectivity of tannin antibiofilm against polymicrobial biofilm were analyzed by calculating minimum biofilm inhibitory concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The mechanism of action of tannin against polymicrobial biofilm was tested using scanning electron microscopy (SEM). The data were analyzed using the Statistical Package for the Social Sciences (SPSS) with a 95% confidence level. Tannin 1% gave inhibition activity of mono-species biofilm formation S. aureus in the middle phase and maturation of 79.04±0.01, 61.48±0.03, E. coli 74.56±0.01, 67.91±0.02, P. aeruginosa 67.32±0.05, 35.13± 0.01, C. albicans 60.62±0.01, 47.16±0.01. The results also provide evidence that tannin activity can degrade and damage the matrix of extracellular polymeric substance (EPS) polymicrobial biofilms. Hence, tannins can be a potential candidate for new antibiofilm for polymicrobial biofilm.

Influence of Propylene Glycol Concentrations in Mangostin Pericarp Extract Gels Formulation: Gels Physical Characteristics, Antibacterial Activity Against Staphylococcus aureus, and Functional Antioxidant Activity Based on Radical 2,2-diphenyl-1-picrylhydrazyl Scavenging Activity Rina Kuswahyuning; Sylvia Utami Tunjung Pratiwi; Joselin Ekaputri; Meiliana Meiliana
Majalah Obat Tradisional Vol 25, No 3 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.54604

Mangostin (Garcinia mangostana L.) fruit pericarp (GMP) extract has been shown to have antimicrobial and antioxidant activities. This study aimed to formulate and evaluate gel formulations of GMP extract using sodium carboxymethylcellulose as a gelling agent and propylene glycol (PG) in varying concentrations (i.e. 0-40%). GMP extract was evaluated for total mangostin content and antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Physical gel characteristics were also examined. All gels had similar pH and spreadability, however, gel with 40% PG produced significantly lower viscosity. Antibacterial activities against S.aureus were shown to be the same among GMP extract gels. The antioxidant activity of all GMP extract gels could be confirmed based on the radical DPPH scavenging method. PG content of 40% significantly reduced the increase of GMP extract gel viscosity after being stored for four weeks at room temperature. Additionally, the lowest syneresis was also shown for GMP extract gel with 40% PG content.

Kaempferia galanga L. Rhizome As a Potential Dental Plaque Preventive Agent Triana Hertiani; Sylvia Utami Tunjung Pratiwi; Iramie Duma Kencana Irianto; Aini Febriana
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev1iss1pp19-25

Dental plaque prevention can be achieved by inhibition of mouth cavity microbes to built biofilm. Kaempferia galanga rhizome has been known as a potential antibacterial agent. This research aimed to reveal the potency of Kaempferia galanga extract and essential oil as anti plaque active agents, based on their in vitro inhibitory activity against the planktonic growth and biofilm of Streptococcus mutans ATCC 21752. Kaempferia galanga extract was obtained by defatting dried-pulverized samples in petroleum ether prior to immersion in 70% ethanol. The fresh rhizome was steam-hydro distilled for 6 h to yield the essential oil. Antibacterial and anti biofilm assays were measured by micro dilution technique on polystyrene 96-wells micro titer plates at 37°C. The percentage of inhibition was calculated by comparing the absorbance of samples to the vehicle (control) measured by micro plate reader at 595 nm. Biofilms formed were first stained by 1% crystal violet. The above assays were performed in triplicates. This study revealed that both K. galanga rhizome essential oil and ethanolic extract showed antibacterial and antibiofilm activity towards S. mutans. The ethanol extract showed MIC90 value at 0.091% w/v and MBC at 2.724% w/v for antibacterial activity; IC50 at 0.048 % w/v for anti biofilm formation activity; and EC50 at 0.052%w/v for biofilm degradation activity. Until the highest concentration tested (0.6%w/v), the MIC90 and MBC values of the essential oil were not revealed, but higher biofilm inhibitory activity i.e. IC50 at 0.025 % w/v; and EC50 at 0.034 %w/v were observed. Key words: biofilm inhibitor, antibacterial, Kaempferia galanga

Penelusuran Potensi Kapulaga, Temu Putri dan Senggugu sebagai Penghambat Pembentukan Biofilm TRIANA HERTIANI; SINTAYU PUTRI WANDAN SARI; FERRY RAHMA PUSPITA; NURI IRIYANI; SYLVIA UTAMI TUNJUNG PRATIWI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.797 KB)

Kapulaga fruit, temu putri rhizome and sengugu bark have been reported as antibacterials, suggesting potency to be developed as antibiofilm agents. This research has investigated the inhibition activity on biofilm formation of Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli. Following petroleum ether extraction, the remaining biomass was macerated in ethanol 70 %. After solvent evaporation, the ethanol extract was partitioned to yield hexane, ethyl acetate and water fractions. The inhibition activities of ethanol extracts, hexane, ethyl acetate and water fractions of samples were carried out in vitro on microplates flexible U-bottom 96 wells using crystal violet staining and were recorded at λ 595nm. Phytochemical identification were performed by thin layer chromatography. Results showed that extract or fraction samples which have the highest biofilm inhibition activity toward MRSA were hexane fraction of kapulaga IC50 0.23±0.01 mg/mL, temu putri IC50 0.45±0.03 mg/mL and the ethyl acetate fraction of senggugu IC50 0.35±0.022 mg/mL. Biofilm inhibition activity toward E. coli, were shown by the ethyl acetate fractions of kapulaga IC50 0.32±0.17 mg/mL, temu putri IC50 0.46±0.03 mg/mL and senggugu IC50 0.39±0.02 mg/mL.

Kontaminasi Bakteri pada Sediaan Campuran Intravena di Bangsal Perawatan Rumah Sakit Shinta Sari Dewi; Fita Rahmawati; Sylvia Utami Utami Tunjung Pratiwi
Jurnal Sains Farmasi & Klinis Vol 5, No 1 (2018): J Sains Farm Klin 5(1), April 2018
Publisher : Fakultas Farmasi Universitas Andalas

Administration of intravenous admixtures with bacterial contamination during preparation and injection to the patient can cause nosocomial infection. The purpose of this study is to determine the frequency of bacterial contamination and the types of the bacteria which contaminate to the intravenous admixtures. The sampling was carried out in the treatment ward, Government Hospital in Yogyakarta. The bacterial contamination was determined by using sterility test with direct inoculation method. A total of 60 intravenous admixtures were collected consisting of 43 intravenous admixtures were prepared by nurse in treatment ward and 17 intravenous admixtures were prepared by pharmacist in clean room. The frequency of bacterial contamination was identified in 1 of 43 (2.3%) prepared in treatment ward and none of the intravenous admixtures (0%) prepared in clean room. Enterobacter cloacae and Staphylococcus aureus were detected in one sample. Although sanitation and the training of aseptic technique can reduce the risk of bacterial contamination in treatment ward, but by using clean room to prepare intravenous admixtures can be the best strategy

Efek Saponin Terhadap Penghambatan Planktonik dan Mono-Spesies Biofilm Candida albicans ATCC 10231 pada Fase Pertengahan, Pematangan dan Degaradasi Hasyrul Hamzah; Triana Hertiani; Sylvia Utami Tunjung Pratiwi; Titik Nuryastuti
Majalah Farmaseutik Vol 17, No 2 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v17i2.54444

Saponin merupakan salah satu jenis metabolit sekunder dari tanaman yang memiliki aktivitas antibakteri namun aktivitas antibiofilmnya terhadap C. albicans ATCC 10231 belum pernah dilaporkan. Penemuan kandidat antibiofilm baru terhadap biofilm C. albicans menjadi tantangan yang harus di atasi dalam mencegah infeksi yang berhubungan dengan biofilm. Penelitian ini bertujuan untuk mengetahui efektivitas saponin dalam menghambat dan mendegradasi C. albicans ATCC 10231. Pengujian planktonik, penghambatan biofilm dan pengujian degradasi biofilm ditentukan dengan menggunakan metode microtiter broth. efektivitas antibiofilm saponin terhadap biofilm dianalisis dengan menghitung minimum biofilm inhibitor konsentrasi (MBIC50) dan nilai minimum biofilm eradication concentration (MBEC50). Senyawa saponin 1 % memberikan aktivitas penghambatan pada fase planktonik C. albicans sebesar 79,48 % ± 0,01, fase pertengahan biofilm sebesar 56,00 % ± 0,02, fase pematangan 35,55 ± 0,03 Hasilnya juga memberikan bukti aktivitas saponin dapat mendegradasi 50 % biofilm C. albicans sebesar 51,03 ± 0,01. Oleh karena itu, senyawa saponin potensial untuk dikembangkan sebagai kandidat obat-obat antibiofilm baru terhadap biofilm C. albicans.

Antibacterial Activity and TLC-Densitometric Analysis of Secondary Metabolites in the Leaves of the Traditional Herb, Melastoma malabathricum L. Dian Mayasari; Yosi Bayu Murti; Sylvia Utami Tunjung Pratiwi; Sudarsono Sudarsono
Borneo Journal of Pharmacy Vol. 5 No. 4 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i4.3818

Indonesia is rich in the biodiversity of medicinal plants used traditionally for healing several ailments. Melastoma malabathricum L. is one of the traditional herbs used to treat many diseases. A TLC-densitometric method was developed for determining secondary metabolites such as phenolic compounds and their related compounds, M. malabathricum leaves from Riau, Indonesia. This study investigated the secondary metabolites of M. malabathricum extract by spraying reagent: FeCl3, p-anisaldehyde, and cerium (IV) sulfate and followed by antibacterial assay through broth macro dilution method. Densitometric qualitative analysis of phenolic compounds and their related compounds was employed to examine peaks of the M. malabathricum extract through winCATS software. After spraying with particular reagents, the three extracts showed various spots/bands with several colors and exhibited peaks in TLC densitogram profiles. Three extracts were tested for antibacterial activity against Staphylococcus aureus and Escherichia coli. The result showed that ethyl acetate extract revealed excellent antibacterial activity against S. aureus and E. coli with MIC values of 3.125±0.6 mg/mL and 6.25±0.5 mg/mL, respectively. Owing to the presence of a wide variety of secondary metabolites, the leaf extract of M. malabathricum is expected to exhibit and help develop as a therapeutic agent.

Co-Authors Adha, Tasya Hardina Aini Febriana Ardani, Marisya Bakhtiar, Muh. Irham Dian Mayasari Ema Damayanti FERRY RAHMA PUSPITA Fita Rahmawati Fransisca Priskaningtyas Wulandari Gunawan, Dede Reza Hamzah, Hasyrul Iramie Duma Kencana Irianto Joselin Ekaputri Khoirunnissa Hida MAULANA, RIZA Meiliana Meiliana Meta Juniatik Muhammad Subhan Ni'matul Munawaroh NURI IRIYANI Nurul Pangestuti Pratama, Virgiawan Yoga Rafika Sari Rina Kuswahyuning Ronny Martien Shinta Sari Dewi SINTAYU PUTRI WANDAN SARI Sudarsono Sudarsono Titik Nuryastuti Triana Hertiani Yosi Bayu Murti

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