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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 17, No 1 (2011)" : 7 Documents clear
Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense) dengan Trichoderma sp. Albertus Sudirman; Christanti Sumardiyono; Siti Muslimah Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.883 KB) | DOI: 10.22146/jpti.9396

Abstract

The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation). Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4). The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation.
Analisis Variabilitas Genetik Rigidoporus microporus (Jamur Akar Putih) pada Karet dengan Teknik PCR-RFLP Fragment ITS Ria Novianti; Haryono Semangun; Ferry F. Karwur; Martanto Martosupono
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.023 KB) | DOI: 10.22146/jpti.9383

Abstract

The eleven isolates studied fell into two groups, which showed that the fungus did not have high genetic variability. Isolates from diseased rubber trees in shorter vicinities, which were due to pathogen’s vegetative spread through root contact, have close genetic relationship. Meanwhile isolates from far apart trees, either in one estate or from different islands, does not necessarily have distant genetic relationship. It might be due to the fact that long distance dispersal of the disease could also due to rhizomorph contaminating rubber stumps. PCR-RFLP Fragment ITS Techniques can be applied to study genetic relationship of Rigidoporus microporus isolates. It is expected that the technique will be applied with more isolates from different provinces by using additional different restriction enzymes.
Prevalensi Nematoda Parasit pada Pertanaman Pisang di Daerah Istimewa Yogyakarta Siwi Indarti; Bambang Rahayu TP; Siti Subandiyah; Lilis Indarti
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.335 KB) | DOI: 10.22146/jpti.9397

Abstract

A study to determine the prevalence and distribution of plant parasitic nematodes associated with banana was undertaken in banana growing areas at four districts (Bantul, Gunung Kidul, Kulon Progo, and Sleman) of Yogyakarta Special Province. Seven genera of plant parasitic nematodes were found on these area: Criconemoides, Helicotylenchus, Hoplolaimus, Meloidogyne, Pratylenchus, Radopholus, and Rotylenchulus. Genera Hoplolaimus and Meloidogyne were distributed at all districts and occurring in soil and root samples of banana cultivars, Ambon, Kepok, Koja, Klutuk, Raja, Tanduk, and Uter, respectively. Four genera, Hoplolaimus, Meloidogyne, Pratylenchus, and Radopholus were dominant and were found with high level of population than the others on each district. Pratylenchus mostly was found on banana cv Kepok with average population 348,2–2057,3 nematodes on total samples of 5g banana root and 100g soil.
Pemanfaatan Beberapa Kaldu Hewan sebagai Bahan Formula Cair Pseudomonas fluorescens P60 untuk Mengendalikan Sclerotium rolfsii pada Tanaman Mentimun Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.913 KB) | DOI: 10.22146/jpti.9384

Abstract

A research aiming at knowing the potency of several animal broths as organic liquid formula of Pseudomonas fluorescens P60, soaking period of Sclerotium rolfsii sclerotia, and its application method on cucumber stem-end rot was done. Completely randomized design and randomized block design both arranged by factorial were used for in vitro and in planta tests, respectively. The first factor was six kinds of animal broth, i.e., golden snail, local chicken,broiler chicken, catfish, cow bone, and rat. The second one for in vitro test was the soaking period in the Pseudomonas fluorescens P60 formula, i.e., 0, 1, 10, and 100 hours and for in planta one was application methods, i.e., seed soaking or crop spraying. Result of the research showed that the best animal broth as liquid formula for Pseudomonas fluorescens P60 was golden snail broth indicated by suppression of sclerotial germination up to 97.4% after soaking for 100 hours. The best application method to suppress the disease was spraying method showed by suppressed of sclerotial germination, longer incubation period, and suppressed disease incidence and sclerotial late population of 55.79, 147.35, 66.67, and 59.68%, repectively. Spraying the formula could also increase crop height difference, fresh and dry weight of crop, fresh and dry weight of root, and root length to 146.83, 86.62, 112.5, 87.88, 140, and 159.68%, respectively.
Toksisitas Abu Terbang Kayu terhadap Nilaparvata lugens dan Kompleks Predatornya Fransiscus Xaverius Wagiman; Hafiz Fauzana; Kartika Yoga Prasetyani
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.336 KB) | DOI: 10.22146/jpti.9762

Abstract

The brown planthopper (Nilaparvata lugens) is currently a very noxious pest insect and it threatened the self sufficience of rice in Indonesia. Innovation of control technology is urgently needed to be developed. Objective of the study was to determine mortality-effect of the wood fly-ash obtained from pulp factory in Riau against N. lugens and its predator complex. A bioassay of the wood fly-ash was done in the laboratory to determine its activity against the test insects. A randomized complete block design (RCBD) experiment with three treatments —fly-ash 40 kg/ha, botanical insecticide containing of root extract of Derris eliptica, and control— with five replications was conducted at a paddy field in Sleman District Yogyakarta. Results showed that the wood fly-ash was toxic against N. lugens: LD50 at 72 hours after treatment were 4.84 and 43.26 g/m2, respectively. As compared with control and botanical insecticide of D. eliptica, the wood fly-ash was significantly more effective for controlling the N. lugens but relatively safe against predator complex. Dusting of the wood fly-ash at rate of 40 kg/ha effectively reduced population of N. lugens within 2 days but did not significantly reduce population of the predator complex namely spiders (Lycosa sp., Oxyopes sp., Callitrichia sp., Argiope sp., and Tetragnatha sp.), Coccinellidae (Menochilus sexmaculatus and Verania sp.), Cicindelidae (Ophionea sp.), and Staphylinidae (Paederus fuscipes). 
Aktivitas Ngengat Scirpophaga incertulas di Wilayah Kabupaten Klaten Mohammad Yunus; Edhi Martono; Arman Wijonarko; RC Hidayat Soesilohadi
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.658 KB) | DOI: 10.22146/jpti.9389

Abstract

The activities of Scirpophaga incertulas adult from Klaten Regency were studied from April to November 2010. The purposes of this study were to observe the activity of moth after adult emergence, their flying activity, and eggs oviposition. Two hundred pupae were collected from the field, then incubated in the laboratory and observed every hour for 72 hours. Flying activity was observed every hour by counting the number of moths that were found in the light traps. This observation was replicated three times during the dark; from 6:00 p.m. to 6:00 a.m. Oviposition activity of moths was observed by infesting twenty females of insect on the rice plants planted in the polybag and covered with plastic sheet. This oviposition activity was also observed every hour during the dark; from 6:00 p.m. to 6:00 a.m. in order to get the number of egg-laying moths. The results showed that the emergence of S. incertulas moth was mainly from 2:00 to 4:00 a.m., the flying activity was detected mostly from 6:00 p.m. to 1:00 a.m., and the moths ovipositing activity was mainly occured from 7:00 to 11:00 p.m. In addition, we found that the intensity of light affected the number of moths coming into the light trap. The light exposed from the 23 Watt lamp (1,500 lumen) was twice more effective in attracting the moths than the 11 Watt lamp (700 lumen).
Karakterisasi dan Deteksi Cepat Bakteri Penyebab Penyakit Darah pada Pisang Nur Edy; Siti Subandiyah; Christanti Sumardiyono; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2849.392 KB) | DOI: 10.22146/jpti.9390

Abstract

Blood disease of banana is one of the most serious banana disease in Indonesia. Although the disease has became the subject of quarantine it eventually spread and found in most provinces in Indonesia. The aim of this research were to identify the blood disease bacterium (BDB) using morphological observation, biochemical assay, pathogenicity testing of hosts range using infectivity titration and rapid detection by Polymerase Chain Reaction (PCR). The results showed that the blood disease bacterium could be differentiated from Ralstonia solanacearum race 2, the causal agent of Moko disease and R. solanacearum tobacco isolates. BDB isolates were not able to hydrolyze gelatin, Tween 80, starch, and were not able to produce nitrite from nitrate. They were only able to produce acid from galactose and glycerol. The pathogenicity test indicated that the BDB was only able to infect the banana/plantain and was not able to infect tomato, eggplant, and chili. Rapid detection using PCR method showed that the 121F/R primers was able to amplify the BDB genome and was not able to amplify the genome of R. solanacearum tobacco isolates.

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