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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
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Articles 509 Documents
 31   32   33   34   35 
Evaluasi Potensi Parasitoid Penggerek Pucuk Tebu, di Kabupaten Bantul Eddy Mahrub; Sri Ambarwati Amini; Nugrahaning Rahayu
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12403

Abstract

The objective of the research is to evaluate the potency of sugar cane top borer parasitoids whether the parasitoids are available from the released individuals produced previously in the laboratory or other species naturally exist in the field. The result showed that Trichogramma sp. which have been released did not attack sugar cane top borer eggs. Tetrastichus schoenobii and Telenomus rowani observed in the field were seemingly replacing the role of Trichogramma sp. parasitoid previously released in the area. Both the T. schoenobii and T. rowani attacked the sugar cane top borer eggs with parasitism of 34.59% and 24.07% respectively. Larval parasitoids were Elasmus zehntneri, Rhaconotus scirpophagae, Stenobracon nicevillei and Allorhogas sp. while pupal parasitoid was Isotima javensis. Larval parasitism was 0-41.00% averagely. The most dominant parasitoid was R. scirpophagae with parasitism of 18.75% followed by E. zehntneri with parasitism of 17.25% respectively. Total parasitism by all species were 37.59% in young plant and 23.90% in old plant respectively. Pupal parasitism on young and old plants were 13.66% and 8.26% respectively. Those parasitoids were available in the field naturally and performed important potentiality in the natural control of sugar cane top borer. Those above mentioned parasitoids need to be conserved by preventing the ecosystem suffering from any hazardous changes, which may afflict negative effects to the natural enemies.
Morphological and Molecular Observation to Confirm the Taxonomic of Coptocercus biguttatus (Coleoptera: Cerambycidae) on Cloves in Ambon and Part of Ceram Island Mohamad Pamuji Setyolaksono; Suputa Suputa; Nugroho Susetya Putra
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.25748

Abstract

This research was conducted to confirm the species of longhorn beetle (Coptocercus biguttatus) drilling clove stems in Ambon and part of Ceram Island, Moluccas, which has been noted as an important pest. Aim of this investigation was to characterize the species morphologically, and more detailed with molecular technique via mtCO1 gene analysis. The longhorn beetle was taken in Ambon and part of Ceram Island, Moluccas and then was etablished in laboratory with host rearing method on pieces of clove stem. The results showed that C. biguttatus attacking clove stems in Ambon and part of Ceram Island, Maluku was closely related and grouped into same cluster with C. rubripes and P. semipunctata in of New Zealand with 85% homology value. C. biguttatus distributed evenly in all clove planting areas in Ambon and part of Ceram Island. IntisariPenelitian ini dilakukan untuk mengonfirmasi spesies kumbang sungut panjang (Coptocercus biguttatus) yang menggerek batang tanaman cengkih di Pulau Ambon dan sebagian dari Pulau Seram, Maluku menggunakan karakter morfologi dan molekuler berbasis gen mtCO1 untuk sidik kekerabatan. Pengambilan sampel kumbang sungut panjang dilakukan di Pulau Ambon dan sebagian dari Pulau Seram, Maluku dengan cara host rearing pada potongan batang cengkih. Hasil penelitian menunjukkan bahwa C. biguttatus di Pulau Ambon dan sebagian dari Pulau Seram, Maluku berkerabat dekat dan berada dalam satu kelompok dengan C. rubripes dan P. semipunctata di New Zealand dengan nilai homologi 85%. Penyebaran C. biguttatus di Pulau Ambon dan sebagian dari Pulau Seram merata di seluruh area pertanaman cengkih.
Morphological and Molecular Characterization of Rhizoctonia solani Isolates from Two Different Rice Varieties Aisyah Surya Bintang; Arif Wibowo; Achmadi Priyatmojo; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.25469

Abstract

Six isolates of Rhizoctonia solani, i.e. two isolates collected from infected rice plants and four isolates from laboratory collection were studied by using morphological characters and molecular analysis. Un-weighted pair group method with arithmetic mean dendogram constructed based on cluster analysis showed that these isolates were grouped into three clusters at the 0.77 similarity coefficient. Cluster I consisted of BA, BNJ, and NBR isolates with 100% similarity and indicated that those were from AG 1 IA sub group, cluster II consisted of BND, and cluster III consisted of SL1 and SL2. Mycelium was very light brown or whitish with few and moderate sclerotia except SL1 and SL2. Molecular characterization showed that BA, BNJ, and NBR were amplified at 140 bp using Rs1F/Rs2R specific primer for R. solani AG1 IA. All isolates were amplified between 350−400 bp using Rhsp1 primer, meanwhile SL1 and SL2 were not amplified using AG2sp and AG22sp2 primers. Based on Maximum Likelihood tree analysis showed that SL1 and SL2 had high similarity based on ITS sequence data.IntisariEnam isolat Rhizoctonia solani yang berasal dari tanaman padi bergejala dan koleksi laboratorium diuji secara morfologi dan molekuler. Analisis UPGMA dengan koefisien persamaan 0,77 menunjukkan bahwa enam isolat tersebut terbagi atas tiga klaster. Klaster I terdiri atas isolat BA, BNJ, dan NBR dengan kesamaan 100% dan menunjukkan bahwa isolat tersebut berasal dari subgrup AG 1 IA , klaster II yakni isolat BND, dan klaster III terdiri atas isolat SL1 dan SL2. Miselium berwarna putih hingga cokelat muda dengan jumlah sklerotia sedang, kecuali isolat SL1 dan SL2. Uji keragaman secara molekuler menunjukkan bahwa isolat BA, BNJ, dan NBR teramplifikasi pada kisaran 140 bp dengan menggunakan  primer Rs1F/Rs2R yang merupakan primer spesifik dari R. solani AG1 IA. Seluruh isolat teramplifikasi pada kisaran 350−400 bp dengan menggunakan primer Rhsp1, sedangkan isolat SL1 dan SL2 keduanya tidak teramplifikasi oleh primer AG2sp dan AG22sp2. Analisis Maximum Likelihood tree berdasar data sekuen ITS menunjukkan bahwa isolat SL1 dan SL2 memiliki tingkat kesamaan yang tinggi.
Confirmation on Status of Omotemnus miniatocrinitus Chevrolat (Coleoptera: Dryophthoridae) in Snakefruit Growing Areas Rangga Arinta Wisastra; Edhi Martono; Suputa Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.24235

Abstract

-
Penyakit Hawar Pelepah (Rhizoctonia solani) pada Padi dan Taktik Pengelolaannya Bambang Nuryanto
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.22494

Abstract

Rice sheath blight disease caused by Rhizoctonia solani Kuhn (AG-1), is one of diseases currently growing and widespread in rice-producing areas in Indonesia. Widely planting of short-type and many tillers rice varieties with high doses fertilized, especially urea, can increase the severity of sheath blight disease. Sheath blight disease is becoming increasingly important role in the rice production system, especially in intensive rice farming. Therefore, it is necessary to analyze epidemic components affecting sheath blight development to determine their management strategies. The sheath blight disease is observed to develop more severe in low areas (0−200 m above sea levels) than in the moderate and high areas, severity of disease is seen to increase in short-type many tillers rice varieties. Initial inoculum are sclerotia and the mycelia in plant debris that have an important role in the rice sheath blight development. However, sclerotia may fail to germinate due colonized by various kinds of antagonistic bacteria in the soil. Bacteria that are antagonistic to R. solani can be isolated from paddy soil containing compost. Mature compost can suppress germination of sclerotia of R. solani by 14%, while in the mature compost enriched with antagonistic bacteria can suppress the germination of sclerotia by 28%. Relative humidity and temperature around the plant affect the development of rice sheath blight disease. Relative humidity decreased 2.8% when watering is only done by flooding the trench around, and decreased by 4.4% when flooding of land only 1 time per week. Sheath blight control by implementing some components in an integrated epidemic have higher chances of success in suppressing the disease development. IntisariPenyakit hawar pelepah padi yang disebabkan oleh jamur Rhizoctonia solani Kuhn (AG-1), merupakan salah satu penyakit yang saat ini berkembang dan tersebar luas di daerah-daerah penghasil padi di Indonesia. Penanaman secara luas padi varietas unggul tipe pendek beranakan banyak dan dipupuk dengan dosis tinggi terutama urea, dapat meningkatkan keparahan penyakit hawar pelepah. Penyakit hawar pelepah menjadi semakin penting peranannya di dalam sistem produksi padi sawah, terutama di daerah pertanian padi yang intensif. Oleh karena itu, perlu analisis komponen epidemi yang memengaruhi perkembangan hawar pelepah untuk menentukan strategi pengelolaannya. Pengamatan menunjukkan bahwa hawar pelepah berkembang lebih parah di daerah rendah (0−200 m dpl) daripada di daerah sedang dan tinggi, keparahan penyakit terlihat semakin meningkat pada varietas padi tipe pendek beranakan banyak. Inokulum awal berupa sklerosia dan miselium dalam serasah tanaman mempunyai peranan penting dalam perkembangan penyakit di pertanaman. Akan tetapi, sklerosia dapat gagal berkecambah karena dikoloni oleh berbagai spesies bakteri antagonis dalam tanah. Bakteri yang bersifat antagonis terhadap R. solani dapat diisolasi dari tanah sawah yang mengandung kompos. Kompos matang dapat menekan perkecambahan sklerosia sebesar 14%, sedangkan pada kompos matang yang diperkaya dengan bakteri antagonis dapat menekan perkecambahan sklerosia sebesar 28%. Kelembapan relatif dan suhu di sekitar tanaman padi memengaruhi perkembangan penyakit hawar pelepah. Kelembapan relatif menurun 2,8% ketika pengairan hanya dilakukan dengan cara penggenangan pada parit keliling, dan turun sebesar 4,4% ketika dilakukan penggenangan lahan 1 kali seminggu. Teknologi pengendalian hawar pelepah dengan menerapkan beberapa komponen epidemik secara terpadu mempunyai peluang keberhasilan tinggi dalam menekan perkembangan penyakit.
Perbaikan Metode Ekstraksi dsRNA Virus secara Sederhana untuk RT-PCR Tiga Virus Tumbuhan Wiwik Endarsih; Sedyo Hartono; Sri Sulandari
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.26026

Abstract

Replicative form (RF) of RNA viruses are dsRNA structured nucleic acid, always found in plants infected by RNA virus. The principle of dsRNA extraction is based on the different affinity of nucleic acids for the cellulose powder and the specific adsorption in 16.6% ethanol buffer. The study aims to develop the simple dsRNA extraction method for the preparation of RT-PCR detection for Rehmannia mosaic virus (ReMV), Cucumber mosaic virus (CMV), Tomato chlorosis virus (ToCV), and compared with commercial kit. The analysis was performed by quantification of nucleic acid with spectrophotometer, efficiency of method (level of complexity, time, cost per reaction) and sequencing. The RNA concentration with simple methode of dsRNA extraction was lower than kit extraction method but the both methods have same pure RNA result. The PCR and sequencing result showed that viral pathogen of pepper, tobacco, and tomato leaf was CMV, ReMV, and ToCV, respectively with amplicon size at 500, 568, and 360 bp. This method is quite cheap and the RNA quantity is proportional to the commercial kit. The simple method of dsRNA extraction can be proposed for the preparation of RT-PCR detection for CMV, ReMV, ToCV. IntisariReplicative form (RF) virus RNA merupakan asam nukleat berstruktur dsRNA, selalu ditemukan pada tumbuhan terinfeksi oleh virus RNA. Prinsip kerja ekstraksi dsRNA berdasarkan afinitas serbuk selulosa terhadap asam nukleat dan adsorbsi spesifik dsRNA pada konsentrasi etanol 16,6 %. Penelitian ini bertujuan untuk mengembangkan metode ekstraksi dsRNA secara sederhana untuk preparasi deteksi RT-PCR terhadap Rehmannia mosaic virus (ReMV), Cucumber mosaic virus (CMV), Tomato chlorosis virus (ToCV) dan dibandingkan dengan kit komersil. Data yang dibandingkan adalah kuantitas asam nukleat, analisa efisiensi metode (tingkat kerumitan, waktu, biaya per reaksi) serta sekuensing. Konsentrasi RNA hasil ekstraksi metode dsRNA secara sederhana lebih rendah dibanding dengan metode kit, namun kedua metode menghasilkan RNA yang murni. Berdasarkan hasil PCR dan sekuensing disimpulkan bahwa virus penyebab mosaik daun lada dan tembakau serta klorosis daun tomat berturut-turut adalah CMV, ReMV, dan ToCV dengan ukuran amplikon berturut turut 500, 568 dan 360 pb. Metode ini cukup murah dan kuantitas RNA yang dihasilkan sebanding dengan kit komersil. Ekstraksi RNA menggunakan metode dsRNA secara sederhana dapat dikembangkan untuk preparasi deteksi RT-PCR terhadap CMV, ReMV, ToCV.
Interaksi Meloidogyne incognita dan Fusarium solani pada Penyakit Kuning Lada Suryanti Suryanti; Bambang Hadisutrisno; Mulyadi Mulyadi; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.29760

Abstract

Pepper yellowing disease is one of the most important disease of pepper causing the decrease of pepper production. This research was conducted in the screen house and laboratory to determine the major causal agent of leaf yellowing disease of pepper. Meloidogyne incognita and Fusarium solani were isolated from pepper plantation in West Kalimantan. Pepper seedlings Natar 1 cultivars were planted in sterilized soil collected from pepper plantation in Bengkayang, West Kalimantan. Five months-old seedling were inoculated with M. incognita (1000 larvae of 2nd stadium/plant) and F. solani (50 ml of spore suspension with spore density of 106 spore/ml) in several combinations of time inoculation, i.e., F. solani  and then M. incognita, M. incognita and then F. solani, M. incognita together with F. solani, M. incognita only, and F. solani only. The parameters observed were the development of leaf yellowing disease every weeks for five months. The number of gall, and population M. incognita were observed at the end of the observation. The result showed that when M. incognita was inoculated  to the roots followed by F. solani, the disease severity and the percentage of plant diseases were higher than those which were infected with F. solani or M. incognita alone. The higher population densities of M. incognita and a number of root gall, had observed on plants inoculated by M. incognita combined with F. solani than plants inoculated by M. incognita and F. solani alone. Interaction between M. incognita and F. solani as caused of leaf yellowing disease of pepper was synergistic reaction. IntisariPenyakit kuning lada merupakan salah satu penyakit penting pada lada yang mengakibatkan terjadinya penurunan produksi lada di Indonesia. Penelitian ini bertujuan untuk mengetahui peran Meloidogyne incognita dan Fusarium solani sebagai penyebab penyakit kuning lada. Penelitian yang dilakukan rumah kasa dan laboratorium. Meloidogyne incognita dan Fusarium solani diisolasi dari pertanaman lada di Kalimantan Barat. Penelitian dilakukan dengan menggunakan bibit lada kultivar Natar 1 berumur 5 bulan, dan diinokulasi dengan M. incognita sebanyak 1000 larva stadium 2 dan 50 ml suspensi mikrokonidium F. solani dengan kerapatan 106/ml. Perkembangan gejala penyakit diamati setiap minggu selama 5 bulan, dan pada akhir pengamatan dilakukan penghitungan jumlah puru dan populasi M. incognita. Hasil penelitian menunjukkan bahwa apabila M. incognita menginfeksi akar dan selanjutnya diikuti dengan infeksi oleh F. solani, tingkat keparahan penyakit dan persentase tanaman sakit lebih tinggi dibandingkan dengan infeksi oleh F. solani atau M. incognita secara terpisah. Populasi M. incognita dan jumlah puru akar pada tanaman yang diinokulasi dengan M. incognita bersama-sama dengan F. solani lebih tinggi dibandingkan pada tanaman yang diinokulasi dengan M. incognita atau F. solani secara terpisah. Interaksi antara M. incognita dan F. solani dalam menyebabkan penyakit kuning lada adalah bersifat sinergis.
Confirmation on Status of Chaetocnema basalis (Coleoptera: Chrysomellidae) as A Vector of Stewart Wilt Disease Heri Widodo; Arman Wijonarko; Witjaksono Witjaksono; Suputa Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.23002

Abstract

Chaetocnema pulicaria and C. denticulata are recognized as vectors of Stewart wilt disease caused by Pantoea stewartii on maize. These insects have not been reported yet in Indonesia, but Stewart wilt disease has been reported in Java and Sumatera Islands. Genus Chaetocnema which presented in Indonesia is C.basalis. It is not cleared whether C. basalis is a vector for Stewart wilt disease like C. pulicaria and C. denticulata. This reseach was aimed to conduct the confirmation on status whether C. basalis have a role as vector of Stewart wilt disease on maize or not. C. basalis imago were collected from maize growing areas in Yogyakarta, and then starved for 24 h. Treatments were applied by placing imago of C. basalis on infected-P. stewartii plants for 72 h. Five insects were then transferred to each plot of healthy plant (1 plot consisted of 5 plants) for 72 h. For control, imago of C. basalis were put on healthy plants for 72 h and five insects were then transferred to other healthy plant (1 plot consisted of 5 plants) for 72 h. Each treatment was repeated three times. On the fifteenth days after transmission, PCR assays were carried out on leaf samples and isolates of bacteria. All sampled leaves analysis showed that there were no Stewart wilt diseases transmission based on PCR assay and bacterial isolates. This concluded that C. basalis is not a vector for  Stewart wilt disease on maize. IntisariChaetocnema pulicaria dan C. denticulata merupakan serangga vektor penyakit layu stewart yang disebabkan oleh bakteri Pantoea stewartii pada tanaman jagung. Kedua serangga ini belum pernah dilaporkan keberadaannya di Indonesia tetapi penyakit layu stewart telah ditemukan di pulau Jawa dan pulau Sumatera. Serangga Genus Chaetocnema yang ada di Indonesia adalah Chaetocnema basalis. C. basalis belum diketahui secara pasti sebagai vektor penyakit layu stewart seperti halnya C. pulicaria dan C. denticulata. Penelitian ini bertujuan untuk melakukan konfirmasi status apakah C. basalis berperan sebagai vektor penyakit layu stewart pada tanaman jagung atau tidak. Serangga uji berupa imago C. basalis yang dikoleksi dari pertanaman jagung di Yogyakarta, lalu dilaparkan selama 24 jam. Pengujian perlakuan dilakukan dengan menempatkan imago C. basalis pada tanaman terserang P. stewartii selama 72 jam. Kemudian dipindahkan pada tanaman sehat sejumlah 5 ekor per plot tanaman bersungkup (1 plot terdiri dari 5 tanaman) selama 72 jam. Perlakuan kontrol dilakukan dengan menempatkan imago C. basalis pada tanaman sehat selama 72 jam, kemudian dipindahkan pada tanaman sehat yang lain sejumlah 5 ekor per plot tanaman bersungkup (1 plot terdiri dari 5 tanaman) selama 72 jam. Masing-masing perlakuan diulang sebanyak tiga kali. Pada hari ke-15 setelah penularan, dilakukan uji PCR daun tanaman sampel dan isolat bakteri. Hasil pengujian semua sample daun menunjukkan negatif sehingga dipastikan bahwa C. basalis bukan merupakan vektor penyakit layu stewart pada tanaman jagung.
Characterization of Pantoea ananatis Isolated from Garlic and Shallot Nanik Nurjanah; Tri Joko; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.27407

Abstract

The new disease on garlic (Allium sativum) and shallot (A. cepa L. aggregatum group) have been found in several production centers of garlic and shallot in Tawangmangu and Temanggung, Central Java. The infected plants showed symptoms of leaf blight accompanied by chlorosis. The objective of this study was to determine the pathogen that causes leaf blight and chlorosis based on the phenotypic characterization and gyrB gene sequences analysis. The research started from the isolation of pathogen, physiological and biochemical test, DNA extraction, and sequence analysis of gyrB using gyrB 01-F and gyrB 02-R primer. The results showed that the isolated bacterial pathogen have a yellow pigment, slimy colonies with regular borders, convex, gram-negative, non-spore, facultative anaerobic, motile, catalase production, indole production, and acid production from D-glucose, D-mannitol, sucrose, and lactose. From the pathogenicity test, it was found that the bacteria produced the typical symptom of leaf blight. Characterization of pathogens based on gyrB gene sequence revealed that the pathogen was placed in the group of Pantoea ananatis. IntisariPenyakit baru pada bawang putih (Allium sativum) dan bawang merah (A. cepa L. aggregatum group) telah ditemukan di beberapa sentra produksi bawang putih dan bawang merah di Tawangmangu dan Temanggung, Jawa Tengah. Tanaman yang terinfeksi menunjukkan gejala hawar daun disertai klorosis. Tujuan penelitian untuk mengetahui karakter patogen berdasarkan fenotipik dan sekuen gen gyrB. Penelitian dimulai dengan isolasi bagian tanaman yang sakit, uji fisiologi dan biokimia, ekstraksi DNA dengan metode CTAB/NaCl dan amplifikasi gen gyrB menggunakan primer gyrB 01-F and gyrB 02-R. Hasil uji menunjukkan koloni berlendir, cembung, pigmen berwarna kuning, gram negative, tidak berspora, aerob fakultatif, motil, produksi katalase, indol, membentuk asam dari D-glukosa, D-monnitol, sukrosa dan laktosa, dan patogenesitas positif. Karakterisasi patogen berdasarkan sekuen gen gyrB, menunjukkan patogen hawar daun berkerabat dekat dengan Pantoea ananatis.
Impact of Abamectin on Anagrus nilaparvatae, An Egg Parasitoid of Nilaparvata lugens Edi Eko Sasmito; Y. Andi Trisyono; Tri Harjaka
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.24759

Abstract

Anagrus nilaparvatae (Hymenoptera: Mymaridae) is an egg parasitoid potential for controlling the major pests on rice, the brown planthopper (Nilaparvata lugens [Hemiptera: Delphacidae]).Abamectin is one of insecticides registered for N. lugens. The research was aimed to investigate the impact of contact application of abamectin on the parasitism level of A. nilaparvatae under laboratory conditions. Adults of A. nilaparvatae and the first instars as well as adults of N. lugens were exposed to the residue of abamection inside the test tube. A. nilaparvatae was much more susceptible to abamectin compared to N. lugens. Application of abamectin at the recommended concentration (22.78 ppm) for 30 min caused 100% mortality, and it reduced to 85% when the concentration was decreased to 0.36 ppm. In contrast, the mortality for the first instar of N. lugens was only 15% at 22.78 and no mortality at 0.36 ppm. No N. lugens adults died even when they were exposed to 22.78 ppm. Furthermore, the parasitism test was conducted using 38 days after planting of IR-64 rice variety. Those plants were infested with 50 females of N. lugens for 2 days. A. nilaparvatae were exposed by contact to 0.02, 0.23, and 2.28 ppm of abamectin. The survivors were released to the rice plant containing eggs of N. lugens. Contact application of abamectin reduced parasitism level of A. nilaparvatae as much as 86.34, 70.01, and 28.43% with concentrations of 2.28 ppm, 0.23 and 0.02 ppm, respectively. In addition, the number of parasitoids emerged decreased with increasing concentration of abamectin. These results suggest that abamectin could be detrimental to A. nilaparvatae due to direct mortality, reduced the parasitism level, and decreased the number of progeny produced. IntisariAnagrus nilaparvatae (Hymenoptera: Mymaridae) merupakan salah satu parasitoid telur yang berpotensi untuk mengendalikan hama utama tanaman padi, wereng batang padi cokelat (Nilaparvata lugens [Hemiptera: Delphacidae]). Abamektin adalah salah satu insektisida yang terdaftar untuk pengendalian N. lugens. Penelitian ini bertujuan untuk mengetahui dampak aplikasi kontak abamektin terhadap suseptibilitas dan tingkat parasitasi A. nilaparvatae terhadap telur N. lugens pada kondisi laboratorium. Imago A. nilaparvatae serta instar satu dan imago N. lugens dipapar dengan residu abamektin di dalam tabung reaksi. A. nilaparvatae lebih peka terhadap abamektin dibandingkan N. lugens. Aplikasi abamektin pada konsentrasi anjuran (22,78 ppm) selama 30 menit menyebabkan mortalitas A. nilaparvatae 100%, dan mengurangi sampai dengan 85% pada konsentrasi yang lebih rendah 0,36 ppm. Sebaliknya, mortalitas instar satu N. lugens hanya sebesar 15% pada 22,78 ppm dan tidak menimbulkan kematian pada 0,36 ppm. Konsentrasi 22,78 ppm tidak menimbulkan kematian imago N. lugens. Selanjutnya, uji parasitasi dilakukan menggunakan media tanaman padi varietas IR-64 umur 38 hari setelah tanam. Tanaman diinfestasi dengan 50 ekor betina N. lugens selama dua hari. A. nilaparvatae dipapar abamektin dengan metode kontak pada konsentrasi 0.02, 0,23, dan 2,28 ppm. Parasitoid yang mampu bertahan hidup dilepaskan pada tanaman padi yang telah diinfestasi telur N. lugens. Aplikasi kontak abamektin mengurangi tingkat parasitasi A. nilaparvatae sebesar 86,34, 70.01, dan 28,43% pada konsentrasi 2,28; 0,23; dan 0,02 ppm. Selain itu, jumlah parasitoid yang muncul semakin menurun dengan peningkatan konsentrasi abamektin. Hasil ini menunjukkan bahwa abamektin dapat merugikan secara langsung terhadap mortalitas serta mengurangi tingkat parasitasi dan jumlah keturunan A. nilaparvatae.

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