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UNEJ e-Proceeding
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Articles 1,005 Documents
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EFFECTS OF DIFFERENT VOLUMES OF NITROGEN GAS FUMIGATION ON POSTHARVEST PERFORMANCES MINIMALLY PROCESSED PINEAPPLE (Ananas Comosus L.) Mubarak A.; Tan G.I.; Wan Zaliha W.S
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Fresh cut pineapple has a high popularity nowadays because of its fresh like quality, readyto-eatpropertiesandhighnutritionalvalue.However,priortopeelingandcuttingprocesses, fresh cut pineapple is usually susceptible to browning incidence, which was catalysed bypolyphenol oxidase (PPO) enzyme. Browning incidence generally degraded the overallattributes of fresh cut pineapple, and simultaneously, limited its shelf life. In present study,the effects of different volumes of nitrogen (N2) gas (0 mL, 5 mL, 10 mL, 15 mL, 20 mL and 25mL) on fresh cut pineapples were evaluated under 5 ºC storage for nine days. The parametersbeing evaluated were browning incidence, flesh colour, total phenolic content, ascorbic acidconcentration, flesh firmness, soluble solids concentration and titratable acidity. It is shownthat N2 gas fumigation at higher volume had significant effect (p≤0.05) in reducing browningincidence of fresh cut pineapple. However, no apparent effects were found in otherpostharvest parameters. In conclusion, fumigation with 25 mL of N2 gas has a tendency indelaying the occurrence of browning, and maintaining other quality attributes of fresh cutpineapple.
STUDY OF VILLAGES MARKET DEVELOPMENT: FOR GENERATING RURAL ECONOMY IN PONOROGO Indriya Radiyanto; Pawana Nur Indah
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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The scope of research put emphasis on the rural market development programtargeting the rural economy as a generator in Ponorogo cross - sectional. This research used astructured survey, direct interviews with the respondents using a questionnaire. Number ofVillage Market is a total of 47 (forty seven) Market Village which spread over 18 (eighteen)districts. Physical Condition of the Village Market, is associated with a protective roof fromthe sun or rain, as many as 26 markets (55.32%) are in good condition, a total of 12 markets(25.53%) in the partially damaged and just as much as 9 market (19.15 %) damagedcondition. Perception buyer / village communities with the existence of the village market, asmany as 70 people purchasers (70%) stated often spending to market, as many as 20 peoplewere buyers (20%) stated quite often shopping to the market and there were 10 buyers(10%) said rarely spending to the village market. Perception merchant with the presence ofthe village market, as many as 65 traders (65%) stated that his business in the market hasgood prospects, as many as 30 traders (30%) stated that good business prospects and asmany as five dealers (5%) stated that its business prospects less good. The existence of ruralmarkets are still highly needed by the community (the buyer), especially the communityaround the market, either to meet daily needs, also for sale again or for the productionprocess. Desired institutional traders in the market in Ponorogo, namely the berkelindan withthe aspect of togetherness, security and can help financial difficulties (the capital).
Comparison of β-Glucanases Production by Acremonium sp. IMI 383068 in Batch and Continuous Culture System Jayus Jayus
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Studies on the possible influence of physical environmental factors on extracellular b-glucanase production by Acremonium sp. IMI 383068 were undertaken, in particular to assess the role of varying agitation speed and pO2 levels, since these have been shown in other studiesto affect the yields of other fungal extracellular enzymes. The use of chemostat cultures in this present study meant that the possible influence of these two parameters on enzyme production could be controlled independently of each other and of growth rate. No other similar data on possible factors affecting fungal (1->3)- and (1->6)-b-glucanases are available in the literature, and therefore other fungal extracellular enzyme systems are used here as comparisons for this discussion. To investigate the possible relationship between enzyme production and culture morphology, the HGU values or branching frequencies of the fungal hyphae were assessed. Since substantial conidial production also occurred in these cultures of Acremonium sp. IMI 383068, it was not possible always to distinguish between mycelial branches that were true vegetative hyphae and those eventually forming the short tapered lateral conidiophores that characterize this strain. Hence HGU determinations include both of these two branch types, except where it was obvious that the branch in question was a conidiophore, adjudged by the presence of emerging conidia from it. The major findings of this study will be discused here which were mainly engaged to the relationship between production of (1->3)-b-glucanases and the growth of the fungus, where it was growth rate dependent, while that of the (1->6)-b-glucanase was growth rate independent. The production of  (1->3)-b-glucanases was shear sensitive and also depended on O2 availability in the culture medium. Based on the data from both batch and chemostat culture, it was found that there was no clear relationship between branching frequency of the fungal mycelium and b-glucanase production by the fungus.Keywords: b-glucanases, Acremonium, HGU, batch and chemostat, branching frequency
Imunogenic Protein of Salivary Gland from Anopheles sundaicus Yunita Armiyanti; Moh Mirza Nuryady; Sugeng Setyo Utomo; Teguh Wahju Sardjono; Loeki Enggar Fitri; Kartika Senjarini
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Malaria is still a major problem for developing countries, including Indonesia. One approach to overcome this disease is prevention by vaccination. However, there is still no effective malaria vaccine that is applicable. The ideal malaria vaccine is a combination vaccine that can prevent the pre-erythrocytic cycle, the erythrocytic cycle and transmission process. Salivary vector-based vaccine has the potential to be developed as a malaria vaccine because it can prevent transmission process and also decrease the morbidity of the disease. Saliva from Anopheles contains vasomodulator and immunomodulatory components, that are required in the blood feeding process, but in the same time it could enhance the transmission of the malaria parasite. If the component in the salivary vector can increase pathogen infection, then vaccinating the host with its anti-substances can control the transmission of pathogens (Transmision Blocking Vaccine). Anopheles sundaicus is an important vector of malaria in coastal areas of Java, Bali, Sumatra, Kalimantan and West Nusa Tenggara islands. Repeated exposures of Salivary Gland Extract (SGE) from this vector have been proven to be able to decrease parasitemic rates in mouse model for malaria in our study. The objective of this research is to determine and localize the immunogenic protein from SGE of An. sundaicus as the first step for the characterization of its immunomodulatory component. Mosquito salivary gland protein profile of An.sundaicus was determine by SDS-PAGE. Determination of salivary glands immunogenic proteins was conducted by Western Blotting with IgG from people living from endemic area as primary antibody. Out of 15 bands appeared in SDS PAGE ranging from 24 kD to 138 kD, only two protein bands with  molecular weights of 68 and 37 kDa were the most immunogenic. Those immunogenic proteins were consistent recognized by pooled serum of people as well as by individual response. Keywords: malaria, saliva, vector, immunogenic protein, vaccine
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Anak Agung Istri Ratnadewi; Andika Ade Kurniawan; Wuryanti Handayani
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
Molecular Dynamic Simulation for Thermal Stability Properties of Endo β-Mannanase Enzyme Adi Yulandi; A A Hermosaningtyas; Sheila Sutanto; Antonius Suwanto
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Endo β-mannanase or mannanase hydrolyse the β-D-1,4 mannopyranoside linkages in β-mannan intomanno-oligosaccharides. Four mannanases, named MAN1, MAN2, MAN3 and MAN4, were isolated from palm kernel meal waste have potential as thermostable mannanases. Series of enzymatic assay to characterize enzyme properties may affect longer time and higher cost. Homology modeling and molecular dynamic simulation are reliable and faster alternative assay to determine enzyme properties by analyzing enzymes’ three-dimensional structure. The structureswere constructed using homology modeling approach using Modeller. Template 2QHA was chosen for having more than 98% sequence similarity with targets. The homology models and template were simulated using molecular dynamics software GROMACS 4.6 for 10 ns production time each at 300 K, 323 K and 353 K. Both targets share the same (β/α)8 TIM barrel folding type similar to template 2QHA The basic analysis of molecular dynamic simulation (root mean square deviation and root mean square fluctuation) showed that both enzymes were thermostable, albeit compared to template 2QHA amino acid residues substitution in samples contribute for different thermostable profile. However, MAN2 was appeared to be more stable at high temperature than other samples. Keywords: endo β-mananase, homology modeling, molecular dynamic simulation
Unique Cellulase Enzyme of Bacillus firmus From Organic Waste Purkan .; Sumarsih .; Rachmadani D A
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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The objectives of this research are to isolate cellulolytic bacteria from liquid fermentation of organic waste, to determine the optimum conditions of cellulose enzyme production and characterize of crude extract cellulase enzyme activity, including pH and optimum temperature. Cellulolytic bacteria was isolated with pour plate method using specific media CMC (Carboxymethyl Celullose) and incubated at  the temperature of 370C for 24 hours. The activity of cellulolytic bacteria can be seen by the presence of transparent zone around the place where colony grows. The identification of isolates was done macroscopically, microscopically and biochemistry. Cellulolytic enzyme activity was determined with Miller method to CMC substrate. In this research, 2 isolates of cellulose producer bacteria with the biggest cellulolytic index of 1,903 (DA1) was obtained. Optimum condition of cellulose enzyme production by the bacteria was at hour-24 with the optimum molasses concentration of 0,4% and producing enzyme activity which is 0,04802 U/mL. The cellulose enzyme activity which was produced by Bacillus firmus isolate showed that the crude enzyme has  optimum activity at  pH 5 and temperature of 500C. Keywords: organic waste, Bacillus firmus,  cellulolytic bacteria, cellulose
Biochemical Resistance Mechanism of Several Genotype of Soybean to Rust Diseases Moh Setyo Purwoko; Endang Budi Trisusilowati; Amarullah .
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The research to identify of the content of Fenol and Flavonoid compounds which set as phytoalexin of several soybean genotype from rust diseases. Four genotypes of soybean which were examined at Unej-1, Unej-2 (moderate resistance), Burangrang (tolerance) and Lokon (susceptible). Based on rating code of IWGSR Unej-1, Unej-2 and Burangrang were proved moderate resistance and Lokon which was considered susceptible reality showed moderate susceptible. At Unej-1, Unej 2 and Burangrang which moderate resistance showed an increase of amount of Fenol and Flavonoid compounds greather than Lokon which was moderate susceptible Keywords: Biochemical Resistance, Soybean and Rust diseases
Transformation of Plasmid pET Endo-1,4-β-xilanase from E. coli TOP10 to E. coli BL21 Agung Budi Santoso; Eka Yuni Kurniawati; Anak Agung Istri Ratnadewi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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In this research we successfully transmited Plasmid pET- Endo from E.coli TOP10 to E.coli BL21. Plasmid pET Endo is recombinant plasmid base on pET-30a(+)  inserted with gene of endo-1,4-β-xilanase isolated from bacillus subtilis sp which is originally living in termite abdomen. First step is isolation of plasmid pET-Endo from E.coli TOP10 with alkaline lyses. Denaturation and renaturation of DNA occurred then separated with centrifugation. Plasmid pET-Endo is smaller than Chromosome DNA. Agarose gel electrophoresis confirmed that Plasmid pET-Endo has isolated. Electrogram of pET-Endo show the band in 6022 bp compare to empty plasmid pET-30a(+) 5422 bp. E.Coli BL21 got pretreatment with  CaCl2 solution to make cell competent for transformation. Isolated pET-Endo inserted to E.coli BL21 with heat shock method. Resistance test with antibiotic has done to know the result of transformation. E.coli BL21 contained plasmid pET-Endo will survive in kanamycin agar media. Colony of E.coli BL21 then cultured in liquid media and examinee it’s growth phase. IPTG as gene inducer given after 2,5 hour of inoculation. Crude enzyme tested for xilanase activity and well proved. Isolation of plasmid pET-Endo then running in Agarose gel electrophoresis also confirm good result of transformation.
The Effect of Bitter Melon Extract (Momordica charantia)in Inhibition of NFkB Activation in Leptin treated HUVECS Azham Purwandhono; Candra Bumi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The obesityincreases the riskof morbidityandmortalityof type2diabetesandcardiovasculardisease. Leptin, an adipocytokine that regulate metabolic process, was increase in obesity. Hyperleptinemia activate the transcriptionfactorNuclearFactorKappaB(NF-kB), leading to the occurrence of inflammation. The chronic inflammation in vessels would cause the dysfunction of endothelial cell.Bitter melon (Momordica charantia) has beenused by many peoplefor the treatmentof diseases. Studies showthatbitter melon fruitcontainssaponins(charantins) andalkaloids. This study aimed toinvestigate the effect of bitter melon extract on the level of activated NF-κB. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without leptin as negative control; 2) HUVECs culture with leptin as positive control; and 3 groups given various dosages of bitter melon (0.1 mg/ml; 0.05 mg/ml; 0.01 mg/ml; 0.005 mg/ml; 0.001 mg/ml) and Leptin. Leptin (500 ng/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking obesity condition. Momordica charantiawere given 2 h before leptin treatment. The level of activated NF-KB was measured by ELISA. This study showed that leptin significantly induce NFkB activation and the treatment of bitter melon inhibit NFkB activation significantly (p<0.05). Keywords: Leptin, Endothel, bitter melon, NFkB

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