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Jurnal Kimia (Journal of Chemistry)

jchem
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Published by Universitas Udayana

ISSN : 19079850 EISSN : 25992740 DOI : 10.24843/JCHEM

Core Subject : Science,

Chemistry

Jurnal Kimia (Journal of Chemistry) publishes papers on all aspects of fundamental and applied chemistry. The journal is naturally broad in scope, welcomes submissions from across a range of disciplines, and reports both theoretical and experimental studies.

Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)

Articles 21 Documents

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Search results for , issue "Vol. 9, no. 1 Januari 2015" : 21 Documents clear

IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA TANIN DARI EKSTRAK DAUN TREMBESI (Samanea saman (Jacq.) Merr) SEBAGAI ANTIBAKTERI Escherichia coli (E. coli) Putu Puspita Sari; Wiwik Susanah Rita; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation and identification of tannin compounds from trembesi leaves (Samanea saman (Jacq.) Merr) and its anti-bacterial activity test against Escherichia coli (E. coli) have been done in this research based on the utilization of trembesi leaves to treat diarrhea. Extraction was done by maceration and partition, separation by preparative TLC. The anti-bacterial activity was tested using wells that diffusion method, and the identification of the compounds was done with UV-vis spectrophotometer and FTIR. Maceration with ethanol produced 36.80 g crude ethanol extract. Phytochemical test showed that acetone and water fractions gave positive result for hydrolyzed tannin compounds but acetone fraction revealed more concentrated than water fraction. Anti-bacterial test result showed that the acetone fraction was active towards E.coli with medium activity. Separation of the eluent n-butanol: acetic acid: water (4:1:5) (BAA) gave six isolates but only two isolates (isolate 2 with Rf 0.61, and isolates 3 with Rf.0.65) gave positive results for tannin. These two isolates were relatively pure on TLC purity test and showed weaker anti-bacterial activity compared to the acetone fractions. Identification using UV-Vis spectrophotometer showed that isolates 2 and 3 gave two similar peaks with maximum absorbance at 346.50 nm and 347.00 nm respectively due to n?*and ??* electron transitions, which indicated the presence of C=O and C=C chromophores. Infrared spectra of isolates 2 and 3 revealed peaks that correspond to characteristic functional groups of tannin including –O-H, C-H aliphatic, C=O esther, C=C aromatic, C-O-H, and C-O-C ether.

ANALISIS PRIMER UNTUK AMPLIFIKASI PROMOTER inhA MULTIDRUG RESISTANCE TUBERCULOSIS (MDR-TB) DENGAN METODE POLYMERASE CHAIN REACTION (PCR) I Gusti Ayu Agung Septiari; Putu Sanna Yustiantara; Sagung Chandra Yowani
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The aim of this study was to analyze several primary combinations for amplifying inhA promoter region by in silico and in vitro ways. Primary in silico’s analysis was done by Clone Manager Suite 6 program. fabG gene sequence of M. tuberculosis was downloaded from www.ncbi.nlm.nih.gov (genbank: U66801.1) and used as DNA template. In vitro detection was done by PCR technique using P16 and 86 M. tuberculosis MDR isolates as DNA template. Amplification was done in described conditions: predenaturation at 95°C for 15 minutes, 45 cycle of amplication (denaturation on 94°C for 1 minute, annealing on 54°C for 1 minute 20 seconds dan extension on 72°C for 1 minute 10 seconds) and also post extension on 72°C for 10 minutes. PCR product was detected by agarose gel elektroforesis (1,5%). In conclusion, combination of primary forward (mabA-inhA-promoter-FS) 5’-ACATACCTGCTGCGCAAT-3’ (18 nucleotide) and primary reverse (mabA-inhA-promoter-R) 5’-CTCCGGTAACCAGGACTGAA-3’ (20 nucleotide) (Chen et al., 2011) have met the good criteria of primary combination which was seen from several aspects such as: primary length, Tm value, %GC, stability, number of hairpins, dimers and runs. In vitro detection showed that the primary combination also amplified inhA promoter region with the length of 284 pb

KAPASITAS ANTIOKSIDAN SENYAWA GOLONGAN TRITERPENOID PADA DAUN PRANAJIWA (Euchresta horsfieldii lesch benn) Kadek Ayu Intan Sari; I Wayan Gede Gunawan; Ketut Gede Dharma Putra
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation, identification, and anti-oxidant assay of triterpenoid compound from Euchresta horsfieldii lesch benn) have been conducted. Maseration of 600 g pranajiwa leaves powder with n-hexane produced 6,1 g n-hexane extract which gave yellow to purple colour change with Lieberman-Burchard reagent. The separation with column chromatography using silica gel 60 obtained 3 fractions (F1, F2, F3) which were then analyzed with triterpenoid test. The positive fraction of triterpenoid (F1) produced 2 g yellow isolate. Infrared spectra indicated that the isolate was a triterpenoid, with characteristic functional groups of OH, –CH, C=O, –C=C, –CH2, –CH3, and C–O alcohol. Ultraviolet-visible spectra showed broad peak at 217 nm. The result of anti-oxidant capacity test of triterpenoid was 94,67%, showing that triterpenoid from pranajiwa leaves can be use as an anti-oxidant.

EFEKTIVITAS LUMPUR AKTIF DALAM MENURUNKAN NILAI BOD (Biological Oxygen Demand) DAN COD (Chemical Oxygen Demand) PADA LIMBAH CAIR UPT LAB. ANALITIK UNIVERSITAS UDAYANA Yudith Rizkia Widyawati; Ida Bagus Putra Manuaba; Ni Gst Ayu Made Dwi Adhi Suastuti
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This research was conducted to determine the effectivity of activated sludge to decrease the Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) of waste water produced by the UPT. Lab Analitik Udayana University. This research used varied sediment masses and aeration times in order to determine the optimum conditions of activated sludge and the effectivity. Variation of the masses were 5, 10 and 20 grams and the aeration times were 1; 2; 3; 4 and 5 days. The result showed that both parameters studied decreased as a result of biological activity which oxidizes organic and inorganic compounds contained in the waste water. Five grams sediment within aeration time 4 days were the most optimum condition in decreasing the BOD (up to 75,25 %) and COD (up to 58,08 %). The results of One-way ANOVA showed there were a significant difference between BOD and COD before and after 3 , 4 and 5 days treatment.

PENENTUAN LAJU REAKSI MAKSIMAL (Vmaks) DAN KONSTANTA MICHAELIS-MENTEN (KM) ENZIM LIPASE PANKREAS PADA SUBSTRAT MINYAK KELAPA, MINYAK SAWIT, DAN MINYAK ZAITUN Ketut Ratnayani; A. A. I. A. Mayun Laksmiwati; Maman Sudiarto
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

A main element in the Michaelis-Menten equation is Km, which is typical for a particular enzyme, with a specific substrate at a certain pH and temperature conditions. The aim of this study is determining the difference in the maximum rates (Vmax) and Michaelis-Menten constant (Km) of pancreatic lipase on the coconut oil, palm oil, and olive oil substrates and the most effective hydrolysis by the pancreatic lipases. Km value was calculated by measuring the rate of the catalyzed hydrolysis with various concentrations of pH, temperature, and the optimum incubation time. Before calculating the value of Vmax and Km, the initial rate (v0) was calculated with the titrimetric method. The results showed that Vmax was 2,11 × 10-3 mmol/min on coconut oil substrate; 2,30 × 10-3 mmol/min on palm oil substrates; and 1,60 × 10-3 mmol/minutes on olive oil substrate. While the pancreatic lipase Km values ??were 1,21 × 104 ppm on coconut oil; 2,29 × 104 ppm on palm oil; and 1,60 × 104 ppm on the olive oil. This results suggested the pancreatic lipase catalyzed the hydrolysis was most effective on coconut oil compared with palm oil and olive oil.

AKTIVITAS ANTIOKSIDAN SENYAWA GOLONGAN FLAVONOID EKSTRAK ETANOL DAGING BUAH TERONG BELANDA (Solanum betaceum Cav.) Ida Ayu Raka Astiti Asih; I Wayan Sudiarta; Ade Ayu Wulan Suci
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Terong belanda (Solanum betaceum Cav.) is a good nutrient for health because of its potential as anti-oxidant. Anti-oxidants work by giving one of its electrons to the compounds that are oxidants so that its activity can be inhibited. Extraction was done by maceration and partition, separation and purification with chromatography analysis, identification of active compound using UV-Vis and FTIR spectrophotometer and testing of anti-oxidant activity with diphenylpicrylhydrazyl (DPPH) method. Extraction of 3,950 grams of fresh terong belanda flesh using 5L 70% ethanol produced 178,44 grams ethanol extract. Partition of 100 g ethanol extract gained three concentrated extracts in n-hexane (0.45 g), chloroform (0.37 g) and n-buthanol (3.39 g). The phytochemical test showed that n-buthanol extract contained flavonoids compounds. The result of anti-oxidant activity was showed that ethanol extract and n-buthanol extract had IC50 of 1,302.08 ppm and 606.06 ppm. Column chromatography analysis on n-buthanol extract with mobile phase n-buthanol:ethyl acetate:10% acetic acid (2:7:1) resulted in 7 fractions (F1, F2, F3, F4, F5, F6, F7) and phytochemical test showed that fraction F2 belonged to flavonoids compound. Infrared analysis identified that isolate had –OH, C=O, C-O, C=C aromatic, CH aromatic, and CH aliphatic. Isolate analysis using UV-Visible gained 2 peaks at ? 322 nm (band I) and ? 285 nm (band II) which indicated that the flavonoids groups was flavanone or dihydroflavonol. By using “shifting” reagent the isolate was suggested to contain flavanone group with hyrdoxy groups at C-2’, C-5’, C-6’ and O-glycoside group at C-7 atom.

FRAKSINASI DAN BIOAVAILABILITAS LOGAM BERAT Fe DAN Mn PADA SEDIMEN DI PELABUHAN BENOA Emmy Sahara; Ida Ayu Gede Widihati; I Gede Darma Putra
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

A research on metal fractionation and bioavailability of Fe and Mn in sediments collected from the area of Benoa Bay at Bali has been carried out. The purpose of this study was to determine the fraction of Fe and Mn in the sediments and their bioavailabilities. Fractionations were achieved by the Sequential Extraction methods during which various solutions were employed. Measurement of the concentration of both metals was done by using Atomic Absorption Spectrofotometer with a calibration curve method. The concentrations of total Fe and Mn in the sediment obtained were of 668.2948 mg/kg and 321.5761 mg/kg, respectively. The results of Fe and Mn fractionations in sediments were as follow: the fraction of freely, leachable and exchangeable (EFLE) were of 27.74% and 24.31%, the fractions of Fe/Mn-oxides were of 23.55% and 19.99%, organic and sulfide fraction were of 25.14% and 26.11% while the resistant fraction were of 23,57% and 29,59%, respectively. It Therefore, the results showed that the bioavailable and the resistant fractions for Fe in the sediment were of 76.43% and 23,57%, respectively while for Mn were of 70.41% and 29,59%, respectively

UJI PEMANFAATAN DAUN SIRSAK (Annona muricata L.) DALAM MENGHAMBAT STRES OKSIDATIF PADA TIKUS WISTAR HIPERKOLESTEROLEMIA MELALUI PENINGKATAN AKTIVITAS SUPEROXIDE DISMUTASE Sri Wahjuni; Sri Rahayu Santi; Ni Nyoman Astuti Wulandari
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Oxidative stress is an imbalance condition between free radicals in the body with the anti-oxidant. Superoxide dismutase (SOD) is one of the endogenous anti-oxidant that can neutralize the excess of Reactive Oxygen Species (ROS). The excess of ROS that caused by stres oksidative can also be neutralized with the help of exogenous anti-oxidants. A soursop plant (Annona muricata L.) has anti-oxidant activity, especially in the leaves. This study aims to determine the activity of the leaves of the soursop (Annona muricata L.) in inhibiting the oxidative stress that conducted on the object hypercholesterolemic rats wistar through increasing SOD activity using variations of the extract dose of 50 mg/kg BW; 100 mg/kg BW and 150 mg/kg BW. In this study the dose gives the best result in inhibiting the oxidative stress through an increase in SOD activity was dose of 150 mg/kg BW with percent inhibition of SOD at (81,42% ± 3.91) where the percent inhibition of SOD is directly proportional to the activity of SOD

PIGMEN MERAH DARI JAMUR YANG DIISOLASI DARI TANAH TEMPAT PEMBUANGAN LIMBAH SUSU I D. K. Sastrawidana; Siti Maryam; I Ketut Sudiana
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The use of synthetic dyes in enhancing the consumer appeal of the product is increasing, but most synthetic dyes are toxic, carcinogenic and difficult to degrade, causing environmental pollution. The purpose of this study is to analyze the characteristics of the red pigments produced by fungi isolated from soil contaminated with goat milk. Stages of research include semisolid fermentation using PDA and milk as a supporting medium for pigment production. Pigment analysis involves determining the characteristics of the spectrum as well as the stability of the colour upon pH and temperature treatments. The results showed that the spectrum of the red pigment from the fungus consisted of two peaks at 416 and 493 nm, the colour was stable at a temperature range of 40-90oC and at pH range of 5-8.

PENGOLAHAN LARUTAN DETERJEN DENGAN BIOFILTER TANAMAN KANGKUNGAN (IPOMOEA CRASSICAULIS) DALAM SISTEM BATCH (CURAH) TERAERASI Ni G. A. M Dwi Adhi Suastuti; I Wayan Suarsa; Dwi Kurnia Putra R
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study was aimed to determine the ability of kangkungan plant (Ipomoea crassicaulis) as a biofilter in the treatment process of detergent solution with the use of an aerated batch system (bulk). The processing units were conditioned in a Styrofoam container equipped with an aeration system. A total of 24 L detergent solution and 5 kangkungan plants were applied in the units. As a comparison, a control unit containing the same solution without the addition of kangkungan plants was also carried out. Investigations of the ability of kangkungan in reducing the content of the pollutants which were measured as COD value, surfactant and phosphate levels were run within 30 days of observations. After 30 days it was found that there was a decrease in surfactant and phosphate levels, which were 97.76%; and 90.77%, respectively while the control showed a decrease of 50.79% and 51.53%, respectively

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ni Putu Diantariani

Contact Email jurnalkimia@unud.ac.id

Phone +628123640424

Journal Mail Official jurnalkimia@unud.ac.id

Editorial Address Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Kampus Bukit Jimbaran, Jimbaran, Bali, Indonesia

Location Kota denpasar, Bali INDONESIA

Abstract

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Contact Name
Ni Putu Diantariani

Contact Email
jurnalkimia@unud.ac.id

Phone
+628123640424

Journal Mail Official
jurnalkimia@unud.ac.id

Editorial Address
Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Kampus Bukit Jimbaran, Jimbaran, Bali, Indonesia

Location
Kota denpasar,

Bali

INDONESIA