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International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 160 Documents
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INVITED REVIEWERS Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

INVITED REVIEWER
Surface Water Pollution And Proposed Solutions For Quality Improvement In Kim Xa Commune, Vinh Tuong District, Vinh Phuc, Vietnam Ha Thi Nguyen; Khai Manh Nguyen; Phuong Minh Nguyen; Tú Anh Cai; Tin Trong Nguyen; Dat Nguyen; Duong Khanh Thi Pham; Ly Huong Vu; Anh Quang Tran; Trang Quynh Le; Duong Thuy Nguyen; Nhat Minh Duong
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

The study investigated the current surface water pollution status in Kim Xa Commune, Vinh Tuong District, Vinh Phuc Province, in Northern Vietnam. Water samples were collected from Phan River, fish farms/ponds, and a pig farm’s effluent in Kim Xa Commune and analyzed for water quality parameters, including chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), and total suspended solids (TSS). Results showed that the Phan River water met the national water quality standards (Regulation QCVN 08:2015/BTNMT). However, the COD and TSS concentrations of the pond water and the piggery effluent exceeded their maximum permitted levels (QCVN 08:2015/BTNMT and QCVN 62:2016/BTNMT). Both had the highest COD of 145 and 192 mg/L, respectively. Moreover, the effluent’s TSS was 2.05 times higher than the national standard (QCVN 62:2016/BTNMT, column B). A 500 m2 floating treatment wetland system planted with water hyacinth has been proposed for improving the quality of the piggery effluent discharged to recipient drainage to meet the standards regulated in QCVN 08:2015/BTNMT for surface water
Feasibility of Additional Wastewater Treatment Plants for Water Quality Improvement at Nong Han Lake, Sakon Nakhon, Thailand Pluem Chuaychoo; Natthapong Proysurin
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

This study examined the possibility of additional wastewater treatment plants for the Nong Han Lake, Sakon Nakhon, Thailand. Hybrid constructed wetlands with horizontal and vertical flows (HFCW and VFCW) were selected for efficient nutrient removal, ease of operation and maintenance, and provision of recreational open space. The design is approximately 70,000 m2 (7 ha)—a decision made considering land availability for stabilization ponds where municipal wastewater carried through pipelines or sewerage accumulates, constructed wetland cells, and pumping stations. In HFCW and VFCW, nutrients are removed by the mechanisms of plant uptake and microbial metabolism. Results showed that HFCW reduced organic compounds, such as biochemical oxygen demand (BOD), by more than 90%, and VFCW removed at least 70% of nitrogen from wastewater. In addition, substantial reductions of up to 95% and 80% were obtained for total suspended solids (TSS) and phosphorus, respectively.
Implementation of constructed wetland technology as a nature-based solution for environmental improvement at the upper reach of the Moskva River Shmonin Kirill; Natalia Korshunova; Elizaveta Derevenec; Veronica Volkova; Maria Lazareva; Olga Denisova; Daniil Barbashin; Zlata Bondar; Sergey Kharitonov
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

Moskva River is an important freshwater ecosystem for the capital region of Russia but is under the constant anthropogenic influence that, to many extents, harms its water quality. A solution has been proposed to improve the river water quality with constructed wetlands in the suburbs of Moscow. The model settlement was Ilyinsky, with a population of 500 living near the river. Wastewater volume, pollutant concentration, and mass flow rate were calculated. Furthermore, local terrains and Russian legal requirements were considered in formulating the design. Based on current river water conditions, it is necessary to build a vertical flow constructed wetland with an area of at least 3.94 m2 per person. It was also estimated that treatment efficiency and pollutant flow into the river would decrease, which should lead to improved water quality at the monitoring point ’Rublevo’. In addition, the research found other small settlements without access to sewage treatment plants. After data extrapolation, introducing the constructed wetlands will expectedly lead to higher-quality water on the Rublevo section. For instance, TSS will decrease from 20.88 to 5.93 mg/l, Total Nitrogen from 1.71 to 0.21 mg/l, and BOD5 from 211 to its natural value—a similar potential change is observed from NH4 + (currently, 0.07 mg/l) and Total Phosphorus (0.16 mg/l). In conclusion, the implementation of the constructed wetlands in the region can improve water quality
Water Quality Monitoring and Wastewater Treatment Using the Constructed Wetland Concept at Bungva Lake, Kaysone Phomvihan City, Savannakhet Province, Lao PDR Khoune Sacbouavong; Khonesavanh Norasane; Phoutthasone Khoangvichit; Phukham Niravanh; Thipsomphone Phoutthavong; Khounphamy Lasachack; Souphaphone Komthirath
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

This study aimed to monitor physical, chemical, and biological water qualities to design a concept of a horizontal subsurface flow constructed wetland for wastewater treatment. The wetland is a small artificial wastewater treatment system consisting of one or more shallow treatment cells planted with wetland vegetation, which is usually suitable for warmer climates. The Standard Methods for the Examination of Water and Wastewater, 23rd Edition, published in 2017 by APHH, AWWA, and WEF, was used to analyze the ASPT index and water quality parameters. Temperature, pH, electrical conductivity, COD, BOD, and invertebrates were observed at three points at Bungva Lake Basin on March 16, 2021. Based on invertebrates (a water quality indicator), the three points had a water quality index value of 4.4, 3.8, and 3.6, respectively. It was also found that many human activities (agriculture, small industry, and settlement) and geological features control the lake’s water quality. Therefore, a horizontal subsurface flow constructed wetland is proposed as sufficient wastewater treatment for the lake
Pollutants at Lake Buyan (Bali, Indonesia) and Solutions for Improving Its Environmental State Ni Made Savita Rasjman Rasjman; I Nyoman Adi Khrisna Wijaya; I Made Ary Putra Nata; Ni Putu Ratna Dewi Damayanti; I Wayan Budiasa; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

Lake Buyan in Buleleng, Bali, has various purposes, such as supplying water for crop and livestock farming and the domestic sector. Wastes generated by human activities in its immediate surroundings are increasing population pressures on the lake’s environment. According to Gubernatorial Regulation Number 16 of 2016, the lake’s water quality is in a poor state and is categorized as ‘polluted’. One of the ways proposed in this research to reduce the pollution level was to build a constructed wetland, which is a waste treatment system combining aquatic plants, soil or other growing media, and microbial assemblages. Results showed that 50 sets of vertical flow constructed wetlands with a single capacity of 40 m3 are needed around Lake Buyan. Another applicable design is concentrating wastewater flows through pipes into a 2000 m3 constructed wetland
Vertical flow constructed wetland: a technological innovation for domestic wastewater treatment at Paoay Lake, Municipality of Paoay, Ilocos Norte, Philippines Charlie B Batin; Julius R Pablico; Jacob A Agtarap; Karen Joy G Bonuen; Justine Rose A Teneza; Amabel H Achuela
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2023): Special Issue February (Online First)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

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Abstract

Wastewater has been a major problem for various communities in the Philippines and worsens as the human population trends upward. Paoay Lake, a water body in the northernmost of the country, is not an exception to this problem. It is one of the country’s largest natural lakes and the region’s largest and most accessible freshwater for domestic use. The lake is also used for recreation and ecotourism activities. However, with the growing domestic and agricultural sectors, the already contaminated lake is receiving more wastewater generated by households and farming practices; thus termed domestic wastewater. This research sought to introduce a vertical flow constructed wetland (VFCW) as a technological innovation for domestic wastewater treatment at Paoay Lake, Municipality of Paoay, Ilocos Norte, Philippines. It specifically aimed to (1) determine the socio-demographic profile of the communities around the lake; (2) determine the lake’s physical, biological, and hydrological characteristics based on the available data; (3) introduce vertical flow constructed wetlands for domestic wastewater treatment; and (4) calculate the flow rate of water extraction by the local households to determine the design and dimension of the wastewater treatment facility. Secondary data were used to achieve the first two objectives, which further revealed the lake’s poor water quality. Thus, the introduction of wastewater treatment facilities such as VFCW is deemed very effective and efficient in treating domestic wastewater while considering the lake’s overall characteristics during the planning and design; the VFCW can be replicated for other water bodies in the Philippines
PATHOGENITY TESTING OF THE MUSHROOMS Trichoderma spp. SOME PLANTATION LAND IN CONTROLLING Fusicoccum sp DISEASE OF TOBACCO PLANT Aisar Novita; Efrida Lubis; Le Huu Phuoc
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p04

Abstract

Trichoderma is a soil saprophytic fungus that is naturally parasitic and attacks many types of plant disease-causing fungi or has a broad spectrum of control. This study aims to obtain superior isolates of the antagonist fungus Trichoderma sp from several plantations. This study used a non-factorial completely randomized design consisting of seven treatments with three replications. The treatments tested were: No Treatment (To); Trichoderma sp isolate from Langkat rubber (T1) Trichoderma sp isolate from Palm Oil Langkat (T2), Trichoderma sp isolate from Cocoa Langkat (T3), Trichoderma sp isolate from Percut oil palm (T4), Trichoderma sp isolate from Karo Coffee (T5), Trichoderma sp isolates from Karo cocoa (T6) Parameters observed were identification of the fungus Trichoderma sp., pathogenicity test on tobacco plants and percentage of pathogen inhibition. The results of both macroscopic and microscopic identification of Trichoderma sp isolates from rubber plantations in Langkat area (T1) showed that the highest percentage of inhibition and pathogenicity tests against Pussicoccum sp disease of tobacco plants showed negative results (no necrotic spots).
Identification of Phytochemical Compounds of Ethyl Acetate Extract of Bulung Anggur (Caulerpa Sp.) By Gc-Ms And Toxicity Test On Artemia Salina Leach Shrimp Larvae Ester Giwo; Nguyen Ti Ha; I Gede Putu Wirawan; I Nyoman Wijaya; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v10.i02.p05

Abstract

Sea grape (Caulerpa sp.) is one of the potential types of seaweed. Sea grape potential as a natural ingredient for making medicine. Balinese people use sea grape for consumption. The content of bioactive compounds in sea grape is suspected to be toxic at certain doses. The purpose of this study was to determine the compound content of ethyl acetate extract of sea grape (Caulerpa sp.) and the toxicity on Artemia salina Leach. The identification of the bioactive compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the toxicity test of extracts was carried out using the Brine Shrimp Lethality Test (BSLT) method. The results showed that the ethyl acetate extract of sea grape (Caulerpa sp.) contained 27 chemical compounds, 7 of which had a quality value > 70%, namely Propanoic acid, ethyl ester; n-Propyl acetate; sec-Butyl acetate; Toluene; Acetic acid, butyl ester; Bicyclo [4.2.0] octa-1, 3, 5-triene; and Styrene. The results of the toxicity test of Artemia salina Leach are toxic which had an LC50 value of 44,070 ppm so that they can be used as a larvicide
Effects of Trichoderma Harzianum and Fusarium Solani Inoculant on the Resin Content of Agarwood (Gyrinops Versteegii (Gilg.) Domke) I Made Mega; Ni Luh Kartini; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i02.p01

Abstract

This study aimed to determine which fungal inoculants, Trichoderma harzianum or Fusarium solani in a solid or liquid form, produced the highest resin content in agarwood trees. This experimental study used a randomized completely block design (RCBD) with seven treatments in triplicates: solid Trichoderma harzianum inoculants in pellets(A) and capsules (B), liquid T. harzianum inoculants (C), solid Fusarium solani inoculants in pellets (D) and capsules (E), liquid F. solani inoculants (F), and control or without inoculants (G). Parameters observed were the color and aroma of the infected agarwood and the resin content of the harvested sapwood. Quantitative data obtained from observations/measurements were analyzed statistically with analysis of variance and a subsequent LSD test at a 5% significance level following a significant result. The results showed that treatments using different fungal inoculants had no significant effects on the resin content of agarwood. However, inoculants in pellets and capsules tended to increase the resin content. The resin content of agarwood ranged from 15.97% to 21.53%, and the highest level was obtained from treatment A (solid T. harzianum inoculants in a pellet form)

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