cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota denpasar,
Bali
INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 160 Documents
 12   13   14   15   16 
Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Shchegolkova, Nataliya; Kalimutu, Putu Krisnawan; Wirawan, I Gede Putu; Suada, I Ketut
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p06

Abstract

Indonesia is the third largest cocoa bean producing country in the world after Ghana and the Ivory Coast with current Indonesian cocoa bean production of 600,000 tons per year. The quality of cocoa is still relatively low compared to the harvest potential that should be obtained, such as the Sulawesi III clone which provides a yield potential of 2-2.4 tons per hectare. This study aims to obtain indigenous bacteria from Meko village so that the role of each bacteria can be further investigated. The results of growing bacteria on LB media found 25 isolates which were observed to have quite varied morphology including round and irregular shapes, having rough and dull and slimy surfaces, opaque, cloudy and colored (yellow and reddish) and having flat and rising elevations. Most of the bacteria obtained were rod-shaped, namely 23 were gram negative and three were gram positive. The shape of the cell is dominated by the shape of the rod, about 19 are rod-shaped and the remaining six are round.
Identification and Antioxidant Activity Test of Strawberry Ethanol Extract Rosalinda and Osogrande (Fragaria x Ananassa) Varieties Enjelina, Ni Desak Gede Rista; Wijaya, I Nyoman; Suada, I Ketut; Phabiola, Trisna Agung; Utama, I Wayan Eka Karya; Yudha, I Kadek Wisma; Zhao, Tiejun
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p06

Abstract

Strawberries (Fragaria sp.) have many varieties spread across Indonesia. Pancasari Village, Buleleng is a strawberry producing area in Bali, there are several strawberry varieties cultivated by strawberry farmers in Bali, including the Rosalinda variety which has a sweet taste and the Osogrande has a sour taste. The purpose of this research is to determine the compounds contained in the fruits of these two varieties and to determine their antioxidant activity. Phytochemical analysis was performed using the GC-MS (Gas Chromatography-Mass Spectrometry) method, antioxidant activity was performed using the DPPH method. The results showed that the thick ethanol extract of (Fragaria x Ananassa) the Rosalinda and Osogrande varieties contained the same compound, namely 5-Hydroxymethylfurfural which has a function as an antioxidant, with 7 compounds that have known uses in the Rosalinda variety and 11 compounds in the Osogrande variety. The antioxidant activity of thick ethanol extract of the Rosalinda strawberry fruit is higher with IC50 of 10,05 ppm than the thick ethanol extract of Osogrande variety strawberry fruit with IC50 of 10,47 ppm, which means both are classified as having very strong antioxidant activity.
The Role of Egg Parasitoids to Control Rice Stem Borers (Scirpophaga incertulas) Walker (Lepidoptera: Pyralidae) in Subak Renon, Denpasar-Bali, Indonesia Yuliadhi, Ketut Ayu; Wijaya, I Nyoman; Costa Cruz, Latizio Beni Da; Yudha, I Kadek Wisma; Utama, I Wayan Eka Karya; Cha, Joo Young
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p02

Abstract

Rice stem borers are the main pest and endemic for the rice plants. The intensity of the attacks paneled fluctuating until it Reaches 90% and causes loss and it is requisite to get to pay serious attention. One of Efforts to Overcome it is the implementation of integrated pest management (IPM) through biological control by natural competitors. Research objectives is to know the role of an egg parasitoids in controlling rice yellow stem borer. Research conducted at Subak Renon, Renon Village, South Denpasar District, Denpasar Regency since April to July 2022. Research is done with the survey by truncate leaf rice containing eggs of rice stem borers at random. The sampling done every week since the plants were two weeks old in Ciherang rice plants. Identification of eggs parasitoids observed under microscope used the key determination insects (Kalshoven, 1981) and the percentage of eggs parasitoids is calculated by a formula Nishida and Torri (1970). The result showed that found three species of parasitoid eggs roomates played the role to suppress the development of yellow rice stem borer namely Trichogramma japonicum Ashm., Telenomus rowani Gahan and Tetrastichus schoenobii Ferr with parasitation capacity of 66%. The highest capacity parasitation played by T. japonicum, followed T. rowani and T. schoenobii Ferr.
Effects Of IAA-Containing Bacterial Application and Dolomite Dosage Reduction on the Growth of Emprit Ginger (Zingiber officinalle Var. Amarum) on Ultisol Soils Leana, Ni Wayan Anik; Ramadhanty, Zahra Avira; Sulistyanto, Prasmadji; Khalimi, Khamdan; Pranatayana, Ida Bagus Gde
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p02

Abstract

Java ginger, locally known as emprit ginger, is an herb in the rhizome category with many medicinal uses. Indonesia is one of the largest ginger producing countries in the world, however its production in 2019-2022 tends to be unstable. Marginal soil, such as ultisol, can be utilized to increase the Java ginger production. Dolomite and IAA-producing bacteria are some soil ameliorants that can be applied to improve the soil quality. This study aimed to determine the effects of IAA-producing bacterial application and dolomite dosage reduction as well as identify the bacterial isolates and the optimal dose of dolomite in white ginger cultivation on ultisol. The study employed a completely randomized two-factorial design. The observed parameters include plant height (cm), leaf area (cm2), total leaf area (cm2), number of leaves, number of buds, and leaf greenness. Results show that S3 bacterial isolate had significant effects on plant height, leaf area, number of leaves, and leaf greenness. The dolomite dose of 100% showed the best effects on plant height, number of leaves, and number of buds. The interactions between IAA-producing bacteria and dolomite dosage affected plant height, number of leaves, and number of buds.
Morphological Identification Characteristics of Basil (Ocimum spp.) in Tabanan Regency, Bali, Indonesia Harianja, Shara Yulita; Sudiarta, I Putu; Zhao, Tiejun
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p07

Abstract

Basil (Ocimum spp.) is an aromatic plant and is the wealthiest essential oil-producing genera from the Lamiaceae family. Due to the various phytochemical compounds or secondary metabolites, Basil has the potential of medicinal plant germplasm as a source of biopharmaceuticals. Several types of Basil grow in Bali, especially in Tabanan Regency, but their morphological characteristics have yet to be identified. This study aims to identify the morphological characteristics of the four types of Basil found growing at that location: Kecarum, Selasih, Tulasi, and Ruku-ruku. This research was conducted by characterizing the characteristics of the parts of the basil plant, including the morphology of the crown (Botany), stem (Caulis), flowers (Flos), leaves (Folium), and fruit (Fructus). The results of the characterization showed that there were similarities between the four types of Basil and differences in crown shape, plant height, the direction of branch growth, type of branching, leaf shape, color (leaves, stems, flowers, fruit), leaf tips, and leaf layout. , and the base of the leaf. Based on the morphological identification, Kecarum and Selasih have similar morphology to Ocimum americanum L and Ocimum basillicum. Tulasi and Ruku-ruku are morphologically identical to Ocimum sanctum var. Rama (red holy basils) and Ocimum campechianum. Keywords: Biopharmaceuticals, Basil, Lamiaceae, Morphology, Ocimum spp.
Analysis of Land Suitability for Arabica Coffee in Toba Regency, North Sumatra, Indonesia Nora, Silvia; Kuswardani, Retna Astuti; Mawardi, Surip; Wijoyo, Hadi; Gandaseca, Seca; Novita, Aisar
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p07

Abstract

Coffee is a mainstay plantation commodity in Toba Regency which is managed independently by the community where the land around the Lake Toba area is one of the potential lands in Toba Regency for developing Arabica coffee commodities because geographically Toba Regency is located at an altitude of 900 meters to 2,200 meters above sea level and directly borders Lake Toba. The strong roots of coffee plants make this plant one of the mainstays in preserving forests, restoring critical land, preventing erosion and flooding during the rainy season. The aim of this research is to analyze the land suitability of Arabica coffee plantations in Toba Regency. The method used to analyses the land suitability by using the matching method which was carried out by matching land characteristics with parameters/ criteria for plants based on Land Map Unit (LMU). The research results show that the area of Toba Regency based on the Geographic Information System (GIS) is 207,518.31 ha. Very suitable (S1) land suitability for Arabica Coffee Plants covering an area of 67,844.68 ha (32.69%), an area of 132,030.09 ha (63.62%) has S2 suitability class and an area of 7,643.54 ha (3.68%) has marginal suitability (S3). The potential land suitability class for coffee plants in Toba Regency becomes very suitable (S1) covering an area of 67,844.68 ha or 32.69%, moderate suitable class (S2) with temperature limitation covering an area of 132,030.09 ha (63.62%) and marginal suitability (S3 with temperature limitation) covering an area of 7,643.54 ha or 3.68%. Nutrient deficiencies were corrected by adding phosphorus (P) fertilizer of 4.19 kg P/ha or 9.31 kg SP36/ha or 11.63 kg TSP/ha. Nitrogen (N) fertilizer is 75 kg N/ha or the equivalent of 166,625 Kg Urea/ha. Potassium (K) fertilizer as much as 3 kg KCl/ha. Compost fertilizer is 10.56 tons of compost/ha.
Phytochemical Screening and Acute Toxicity Test of Caulerpa lentillifera J. Agardh Infusion Extract on Mice (Mus musculus L.) Adiyanti, Sekar; Phabiola, Trisna Agung; Sutari, Ni Wayan Sri
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p03

Abstract

Caulerpa lentillifera has ingredients that are beneficial for health, such as anti-cancer, antioxidant, anti-diabetic, and immunostimulatory effects. This study was aimed to determine the content of phytochemical compounds and the LD50 acute toxicity dose of C. lentillifera infusion extracts on mice for their utilization as food or medicine. The phytochemical screening was done with a qualitative method. Twenty-five mice have been used for the acute toxicity test and divided into five groups of treatment (Control; 375 mg/kg BW; 750 mg/kg BW; 1.500 mg/kg BW; 3.000 mg/kg BW C. lentillifera infusion extracts orally). Symptoms of toxicity and mortality were observed 24 hours after treatment, and body weight was observed for seven days. The phytochemical screening result showed that the C. lentillifera infusion extracts contained alkaloids, saponins, and tannins. Acute toxicity tests showed no mortality in all treatment groups. Thus, the LD50 value could not be obtained and was classified as non-toxic.
Effectivity Test of Compost Added By Coca-Cola Solid Waste Sludge With Water Spinach (Ipomoea Reptans POIR.) As an Indicator Arthagama, I Dewa Made; Bimantara, Putu Oki; Gunasih, Ni Made Tri; Narka, I Wayan
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p03

Abstract

Effectivity test of compost fertilizer added with Coca-Cola solid waste sludge with an indicator of water spinach (Ipomoea reptans POIR.), aiming to determine the effectiveness of the dose of compost added with Coca-Cola solid waste sludge to increase the yield of water spinach and improve some soil chemical properties. This research is a pot experiment in a greenhouse that was carried out in Kerambitan village, Tabanan region, Bali, Indonesia. Using a completely randomized design (CRD) consisting of 7 doses of compost added by Coca-Cola solid waste sludge. The compost that was used as a treatment was compost that had been added 20% of Coca-Cola solid waste sludge. The doses of compost tested were: K0 (control), K1 (3 tons of compost), K2 (6 tons of compost), K3 (9 tons of compost), K4 (12 tons of compost), K5 (15 tons of compost), Ka (250 kg urea + 50 kg phonska) per hectare, and each treatment was repeated 4 times so that 28 experimental pots were conducted. Parameters observed included: maximum plant height (cm), fresh plant weight at harvest, relative agronomic effectivities (RAE), oven-dry plant weight, soil pH, soil organic carbon (SOC), and soil CEC at harvest. Observational data were statistically analyzed, to determine the effect of the treatment being tried. If the treatment has a significant effect, then continue with the Duncans 5% test. The statistical analysis showed that the treatments had a significant effect on plant height growth, fresh and oven-dry weight of plants, as well as on some soil chemical properties. The heaviest fresh plant weight was obtained in treatment Ka (98.41 g), followed by K5 (98.35 g), K3 (98.33 g), and K4 (98.21 g) per pot. The highest value of relative agronomic effectiveness (RAE) was obtained in K5 (99.63 %), followed by K3 (99.58 %), and K4 (98.76 %). While the highest CEC was obtained in the treatment of K5 (40.25 me 100 g-1), K3 (39.75 me g-1) with successive organic-C levels (31.75%), (3.16 %) and pH (7.04), (7.0). The best dose of compost treatment from the results of this study was found in the K3 treatment (9 tons of compost ha-1).
ANTIFUNGAL ACTIVITY OF SIAM CITRUS (Citrus nobilis L.) ESSENTIAL OIL AGAINTS Lasiodiplodia theobromae THE PATHOGEN OF BLENDOK DISEASE Pranata, Kadek Dwipayana; Wirawan, I Gede Putu; Wibawa, I Putu Agus Hendra; Suada, I Ketut; Wijaya, I Nyoman; Phabiola, Trisna Agung
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p08

Abstract

Siam citrus (C. nobilis L.) is a type of citrus that is most widely cultivated in Bangli Regency. The main disease that attacks citrus plants is blendok caused by the fungus L. theobromae. Based on research siam citrus peel contains essensial oils that have potential as antifungals. The aim of this study was to study chemical content of essensial oil from siam citrus peel and its activity as an antifungal againts L. theobromae. Essensial oil was destilled using hydro steam distillition and analyzed by means of GC-MS. Antifungal activiy testing was carried out using the agar-well diffusion method with concentrations of 1%, 10%, 25%, 50%, 75%, and 100%. Dithane M45 6 g/l and DMSO 10% were used as positive and negative controls. The results showed that the siam citrus peel from Kintamani, Bangli contains essensial oils with the main chemical components, i.e. D-limonene (57.26%), Beta-pinene (9.09%), and Beta-myrcene (4.03%) which has uses as an antifungal, additive, antitumor, asthma and allergy reliever, repellent, anti-inflammatory, antioxidant, anticancer, and antibacterial. Essential oil concentration of 25 to 100% can inhibit the growth of L. theobromae. The largest diameter of inhibition is shown at a concentration of 100% and the smallest diameter of inhibition is shown at a concentration of 25%. Keywords: C. nobilis L., essensial oils, GC-MS, L. Theobromae, antifungal
Meristem Culture of Strawberry Plants (Fragaria x Ananassa Duch.) Sachinoka Variety on Various Types of Basic Media In Vitro Fitriani, Yuyun; Yuswanti, Hestin; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p08

Abstract

The large number of in vitro media circulating in the market is a challenge to find the most effective media for sachinoka strawberry meristem culture, because the need for in vitro media depends on genetic intelligence. The purpose of this study was to find the best media from the various tested media to reproduce the Sachinoka variety strawberry seedlings in a relatively short time using the meristem culture method. This study used a completely randomized design (CRD) with 6 media treatments and repeated 7 times, namely: M1 (WPM without BA), M2 (WPM + 4 ppm BA), M3 (NP without BA), M3 (NP + 4 ppm BA), M5 (BMM without BA), M6 (BMM + 4 ppm BA). The variables observed were the time of emergence of shoots, the number of shoots and the number of leaves. The results of this study indicate that there is no significant difference for the emergence of shoots variable. Treatment M1 produced the highest number of shoots (12.29) but not significantly different from M5 (10.14), both were significantly different from both treatments. The highest number of leaves is in M1 (27.14). It can be concluded that the best medium for the growth of sachinoka strawberry meristem culture is WPM media with the addition of 4 ppm BA, while the second-best medium is BMM media with the addition of 4 ppm BA.

Page 16 of 16 | Total Record : 160

 12   13   14   15   16 

Filter by Year

2012 2024


Reset
Filter By Issues
All Issue Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology Vol 10 No 2 (2023): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 1 No 1 (2023): Special Issue February (Online First) Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 9 No 2 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 9 No 1 (2021): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY Vol 8 No 2 (2021): International Journal of Bioscience and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020 Vol 7 No 2 (2020) Vol 7 No 1 (2019) Vol 6 No 2 (2019) Vol 6 No 1 (2018) Vol 5 No 2 (2018) Vol 5 No 1 (2017) Vol 4 No 2 (2017) Vol 4 No 1 (2016) Vol 3 No 2 (2016) Vol 3 No 1 (2015) Vol 2 No 2 (2015) Vol 2 No 1 (2014) Vol 1 No 2 (2013) Vol 1 No 1 (2012) More Issue