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Kadek Karang Agustina
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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Prevalensi Infeksi Cacing pada Ikan Pisang-pisang (Pterocaesio diagramma) dan Ikan Sulir Kuning (Caesio cuning) yang Dipasarkan di Pasar Ikan Kedonganan, Badung I Putu Hendra Pradipta; Nyoman Adi Suratma; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.301 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi, intensitas infeksi serta distribusi cacing pada berbagai organ, selain itu dapat juga  melihat hubungan antara jenis ikan dengan prevalensi infeksi cacing pada ikan Pisang - pisang (Pterocaesio diagramma) dan ikan Sulir Kuning (Caesio cuning)  yang dipasarkan di Pasar Ikan Kedonganan, Badung. Metode penelitian yang dilakukan adalah dengan pengamatan secara makroskopis dan mikroskopis mengikuti metode Fernando et al. & Kabata, kemudian data dianalisa secara deskriptif dan menggunakan uji Chi-Square.  Prevalensi dan intensitas dari masing-masing jenis cacing pada ikan Pisang – pisang (Pterocaesio diagramma) yaitu digenea (45,71%) dengan rata-rata 4,81±5,9, cestoda (34,29%) dengan rata-rata 12,5±18,1, Hysterothylacium sp. (2,86%) dan Raphidascaris sp. (2,86%) ditemukan berjumlah 1 ekor, Terranova sp. (8,57%) dengan intensitas 1 ekor setiap ikan,  acanthocephala (42,85%) dengan rata-rata 2,13±1,35, sedangkan ada 2 ekor cacing (5,71%) tidak bisa teridentifikasi. Pada ikan Sulir Kuning (Caesio cuning) dari 35 ekor ikan yang diteliti , prevalensi digenea (82, 86%) dengan rata-rata 5,62±4,6, Hysterothylacium sp. (5,71%) dengan rata-rata jumlah cacing 1 ekor dan Cucculanus sp (5,71%) dengan rata-rata 1,5. Lokasi distribusi cacing yang menginfeksi ikan Pisang - pisang (Pterocaesio diagramma) dan ikan Sulir Kuning (Caesio cuning) ditemukan pada beberapa organ antara lain operkulum, insang, rongga insang, lambung, usus, hati, sekum, gonad dan rongga tubuh. Setelah dilakukan analisis statistik menggunakan uji Chi-Square ternyata prevalensi infeksi tidak berhubungan nyata (P>0,05) dengan jenis ikan.
Efek Ekstrak Daun Ashitaba (Angelica keiskei) Terhadap Gambaran Histopatologi Ginjal Mencit (Mus musculus) Jantan Made Oka Adinata; I Wayan Sudira; I Ketut Berata
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui efek ekstrak etanol daun Ashitaba (Angelicakeiskei) yang diberikan secara oral dengan dosis bervariasi terhadap gambaran histopatologiginjal mencit. Sampel yang digunakan dalam penelitian ini berjumlah 25 ekor mencit(Musmusculus) jantan dengan rata-rata berat badan 25-30 gram dan dibagi menjadi limagrup perlakuan. Perlakuan pertama tidak diberikan ekstrak etanol daun Ashitaba atausebagai kontrol. Perlakuan kedua sampai keempat masing-masing diberikan dosis 125mg/kg berat badan (bb); 250 mg/kg bb; 500 mg/kg bb; dan 1.000 mg/kg bb. Perlakuandiberikan secara oral setiap hari dalam waktu 21 hari. Pada hari ke 22, semua mencitdinekropsi dan ginjal diambil untuk selanjutnya dibuat preparat histopatologi denganpewarnaan hematoksilin eosin (HE). Perubahan histopatologi diperiksa berdasarkan adanyadegenerasi melemak, peradangan dan nekrosis. Hasil dari pemberian ekstrak etanol daunAshitaba (Angelica keiskei) dengan dosis 125 mg/kg bb, dua ekor mencit mengalamidegenerasi melemak dan infiltrasi sel radang dan satu mencit mengalami nekrosis.Pemberian dosis 250 mg/kg bb, tiga ekor mencit mengalami degenerasi melemak daninfiltrasi sel radang dan satu ekor mencit mengalami nekrosis. Pemberian dosis 500 mg/kgbb, tiga ekor mencit mengalami degenerasi melemak dan infiltrasi sel radang dan satu ekormencit mengalami nekrosis. Pemberian dosis 1000 mg/kg bb, tampak adanya infiltrasi selselradang, degenerasi melemak dan nekrosis pada semua mencit yang digunakan.Simpulan penelitian adalah pemberian ekstrak etanol daun Ashitaba pada dosis 1000 mg/kgbb dapat menimbulkan gangguan gambaran struktur histopatologi ginjal mencit (Musmusculus) jantan.
Idenfitikasi Bakteri Asam Laktat Isolat 9A Asal Kolon Sapi Bali secara Fenotipik Mita Ekamelinda; I Wayan Suardana; Komang Januartha Putra Pinatih
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.904 KB) | DOI: 10.24843/bulvet.2018.v10.i02.p14

Abstract

Lactic acid bacteria are the type of bacteria that has benefits in food and health industries as a biopreservative, fermentative, or probiotics. Bali cattle are known as potential host for specific lactic acid bacteria. The aim of this study is to identify phenotypically lactic acid bacteria 9A isolated from bali cattle’s colon, that producing a substance which known has potency as antimicrobial. In this study, phenotypic identification included conventional method and API 50 CHL. The result of this study showed that lactic acid bacteria isolate 9A was Streptococcus sp., whereas identification by kit API 50 CHL showed isolate 9A as Lactobacillus fermentum with 83% identity. The difference between the results of conventional method and kit API 50 CHL, may indicate the difference in sensitivity and specificity of the two methods, hence it needs further confirmation.
Aktivitas Biologis Imunoglobulin Yolk Anti Parvovirus Setelah Perlakuan Suhu I Gusti Ayu Agung Suartini; Ni Luh Putu Agustini; Ni Luh Eka Setiasih; Sisyawati Putriningsih; Defi Lega Nurwidana
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study aims to determine the effect of temperature on the biological activity of various crude and precipitate specific Immunoglobulin (Ig)Y Canine parvovirus (CPV). Hiperimun serum conducted on Isa Brown chickens injected with antigen CPV. Crude yolk Ig preparations derived from chicken serum without purification while the yolk Ig preparations precipitates obtained by the chicken serum was precipitated with ammonium sulfate and dialyzed. Both types of Ig yolk given treatment temperature 50ºC, 60ºC, 70ºC, and 80ºC for 15 minutes. To test Gel Precipitation Test (AGPT) is performed to determine whether there is a specific IgY CPV in the serum of chickens. Biological activity of both types of Ig detected with Barriers Haemagglutination test (HI). The design used in this study is completely randomized design factorial. The results of this study indicate that the temperature was highly significant on the activities of IgY crude and precipitates. Activities IgY crude and precipitate down to the treatment temperature of 50ºC, 60ºC, 70ºC, and 80ºC. Geometric Mean Titer crude IgY respectively - were 26.67, 26, 25.33, and <2º Unit HI while IgY precipitates are respectively 26.33, 25.67, 24, and <2º Unit HI. Based on the results of this study concluded that the biological activity of crude IgY better than IgY precipitates after treatment of a wide range of temperatures.
DETEKSI HISTOLOGIK KESEMBUHAN LUKA PADA KULIT PASCA PEMBERIAN DAUN MENGKUDU (Morinda Citrofilia Linn) Ni Ketut Suwiti
Buletin Veteriner Udayana Vol 2 No. 1 Pebruari 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

A study to detect the microscopic structure of skin wound in mice (Mus musculus) aftertreatment with mengkudu leaves (Morinda citrifolia Linn.), by histological methods, hasbeen carried out. In this study were detected the level of wound healing every weeks. Thesamples were collected from skin with was given incision injury. The tissue samples werefixed, dehydrated and embedded in paraffin and 4 – 5 ?. sections. Harris-Haematoxilin-Eosin staining method, using to identified of histological structure.Microscopic analysiswas performed using a binocular light microscope (450X). The study showed that,histological structure of normaly skin we deteted in fourth weeks ware composed byepidermis, dermis and hipodermis tissue. We observed for the presence ofthe ephitelialsquomous complexs, hairfollicles, connective tissue with vein and arteriole and adiposetissue.
Karakteristik Fisik Daging Sapi Bali dan Wagyu Ni Ketut Suwiti; Ni Nyoman Citra Susilawati; Ida Bagus Ngurah Swacita
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.282 KB) | DOI: 10.24843/bulvet.2017.09.02.125

Abstract

A research has been done in order to determine a physical characteristics i.e water holding capacity, acidity level (pH), and cooking loss of beef from bali and wagyu cattle which were sold in supermarkets around Denpasar. The sampels used in this study were 15 rib eye part of bali and 15 of the wagyu beef respectively, the sampels from both types were tested on the water holding capacity parameter, the acidity level, and cooking loss. The data were analyzed by qualitative descriptive analysis. The result showed that, the bali beef contained 66.13 % water holding capacity, 5.75 of acidity level and 31.74 % of coking loss, whereas the wagyu beef contained 63.84 % water holding capacity, 5.20 of acidity level and 32.29% of cooking loss.
Prevalensi Infeksi Cacing Ancylostoma Spp Pada Kucing Lokal (Felis catus) Di Kota Denpasar Putu Anna Oktaviana; Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 6 No. 2 Agustus 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

A long time ago local cat (Felis catus) is a symbol of faith and now it turns to mice controller or even pet. The efficiency of maintains and food make more people interested to take care of cat. Based on the area of living, cat can be categorized into three such as: (1) Domestic pet cats , (2) Stray cats  and (3) Feral cats. Domestic pet cats can be categorized into three also based on authority area such as  Indoor, Limited range and Free range. Cat disease can cause by parasite, virus, bacteria and fungus. There are some species of parasite which often be found in cat digest track such as Ancylostoma spp,  Toxocara spp and Strongyloides spp. The purpose of this research is to know the prevalence of Ancylostomma spp. infection in local cat. This research use 80 samples which divide into 40 stray cat feces and 40 home cat feces using floatation method. From 40 samples of examinated stray cat feces, 19 samples (47.5%) positively found the egg of Ancylostoma spp. Meanwhile from 40 samples of examinated home cat feces, 10 samples  (25.0 %)  positively found the egg of Ancylostoma spp. After analyzed by Chi-square test, there is a clear relation between the prevalence of Ancylostoma spp. infection in home cat and stray cat. (P<0,05)
GAMBARAN SEL DARAH MERAH SAPI BALI (STUDI RUMAH POTONG) Siswanto -
Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

A study to observed the profile of erythrocytes of bali cattle was conducted from 2000 to2007 years at Veterinary Physiology Laboratory, Faculty of Veterinary Medicine, UdayanaUniversity. The aim of this observation were to study and evaluated erythrocytes andcomplete the data. A total of samples is one hundred ninety-two blood samples were takenfrom bali cattle were slaughtered at the abattoir of Sanggaran Denpasar. The parametersobserved were the number erythrocytes (Hemocytometer Method), hemoglobineconsentration,(Sahli method) PCV, MCV (fl), MCH (pg), and MCHC (%/dl) usingMicrohematocrite method. Results of this observation showed that there ware totalerythrocytes 5,2 million/mm3, hemoglobine consentration 8,7 gr%, PCV 27,2 %, MCV56.2 fl, MCH 16.7 pg, and MCHC 29.8 %/dl.
Kadar Logam Berat Pb dan Histopatologi Limpa Sapi Bali yang Dipelihara di Tempat Pembuangan Akhir Suwung Denpasar Wahyu Semadi Putra; I Ketut Berata; I Made Kardena
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1215.95 KB) | DOI: 10.24843/bulvet.2018.v10.i01.p10

Abstract

Bali cattle eats organic and inorganic waste in Suwung Denpasar landfill as source of feed. The waste was suspected contaminated by heavy metals Pb. The aim of this study is to determine levels of heavy metals Pb and spleen histopathologic changes of bali cattle reared in Suwung Denpasar landfill. Spleen samples were collected from 5 cattle for examination of heavy metals Pb by Atomic Absorption Spectrofotometri method and histopathology preparations made by Hematoxilin-Eosin staining. The results showed the presence of heavy metal Pb contamination which varied of 1.5024 ppm, 1.5002 ppm, 2.0267 ppm, 2.0268 ppm and 0.6818 ppm. Microscopically, showed histopathological changes the form of depletion and proliferation of lymphoid cells.
Isolasi dan Identifikasi Escherichia coli O157:H7 pada Feses Sapi di Kecamatan Petang, Kabupaten Badung-Bali I Wayan Suardana; Putu Januari Ratna Apsari Putri; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Coliform are commonly found in livestock stool, including cattles and Escherichia coli (E. coli) is one of the most popular of Coliform groups. Various strains of E. coli are pathogenic which oneof them is serotype O157:H7 which known producing Shiga like toxin. The study initiated by collecting 58 samples of fresh cattle stool which were taken from seven villages in the district of Petang, followed by isolating and identificating of strain. The results of study presented indescriptive and the relationship between each vaiable was analyzed by Spearman 's rho and McNemar test. Among 58 samples, all of them were positive Coliform (100 %), and 29 samples positive E. coli (50 %). Among E. coli positive, that were found 7 samples were positive E. coli O157 ( 12.07% ) and 5 samples positive E. coli O157:H7 (8.62 %). The total Coliform and the number of E. coliwere highly significant difference, while the number of E. coli O157 and E. coli O157:H7 were not significant difference. Coliform bacteria in the stool of cattle could not be used as a predictable factor for the presence of E. coli, E. coli O157 and E. coli O157:H7, but the presence of E. coli would be enable to predict the presence of E. coli O157 as well as E. coli O157:H7. Futhermore, the study also showed the presence of E. coli O157 was as a good predictor to the presence of zoonotic agents E. coli O157:H7.

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