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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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REPLIKASI VIRUS DENGUE PADA KULTUR SEL ENDOTEL PEMBULUH DARAH KELINCI Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 1 No. 1 Pebruari 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.177 KB)

Abstract

Infeksi virus Dengue (DEN) dapat menyebabkan dengue hemorrhagic fever –dengue shock syndrome (DHF-DSS), yang ditandai dengan kebocoran plasma dan gangguan hemostasis. Meskipun sel endotel dipertimbangkan dapat menjadi target sel pada patogenesis DHF, namun sedikit bukti yang menyatakan infeksi virus dengue menyebabkan perubahan fungsi sel endotel. Dalam studi ini sel endotel diisolasi dari aorta desenden thoraxis-abdominalis kelinci, kemudian dilakukan kultur sel primer. Kultur sel kemudian diinokulasi dengan virus Dengue DEN-1, -2, -3, -4, dan DEN-mix. Replikasi virus dengue pada kultur sel endotel diukur dengan uji enzyme-linked immunosorbent assay (ELISA). Titer Ag (DEN-1, -2, -3, -4, dan DEN-mix) yang didapat dari supernatan bervariasi. Dengue tipe 2 mempunyai titer paling tinggi dibandingkan dengan DEN-mix dan tipe virus dengue lainnya. Kerusakan sel endotel menyebabkan kebocoran vaskuleryang berperanan pada patogenesis infeksi virus dengue. Hasil tersebut menyiratkan kemungkinan kerusakan sel endotel disebabkan oleh infeksi virus dengue yang mengakibatkan kebocoran vaskuler.ABSTRACTThe severe outcome of the Dengue (DEN) virus infection known as DEN hemorrhagic fever – DEN shock syndrome (DHF – DSS), is characterized by plasma leakage and hemostasis derangements. Although endothelial cells have been speculated to be a target in the pathogenesis of DHF, there has been little evidence on Dengue virus infection to any alteration in endothelial cell function.In this study, the endothelial cells has been isolatedfrom rabbit thoraxis-abdominalis descendentaortha, then performed primary culture. The culture was then inoculated with virus Dengue DEN-1, -2, -3, -4 and DEN-mix.Replications of dengue virus in endothelial cells culture were demonstrated by enzyme-linked immunosorbent assay (ELISA). Ag titers found among the supernatant of DEN-1, -2, -3, -4, and DEN-mix cultures were vary.Dengue type 2 had the highest virus titers in supernatant compared with those of DEN-mixand other types. Endothelial cell damage may couse vascular leakage that contributes to the pathogenesis of Dengue infection. There results imply the possibility that the existence of endothelial cell damage caused by DV infection may cause vascular leakage.
Total Eritrosit, Kadar Hemoglobin, dan Nilai Hematokrit Babi Landrace yang Diberi Pakan Eceng Gondok dari Perairan Tercemar Timbal Putu Vindhy Chempaka Putri; Komang Budaarsa; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The experimental study was done using a completely randomized design, and the aim of this study is to determine the effect of water hyacinth (Eichhornia crassipes) collected from polluted water in feed on the red blood cell (RBC) count, hemoglobin level, and the hematocrit. A total of eight Landrace pigs were used in this study, consisted of four groups: pigs were fed without hyacinth (A), pigs were fed with 2.5% hyacinth (B), pigs were fed with 5.0% hyacinth (C), and pigs were fed with 7.5% hyacinth (D). Blood were then collected from auricularis superficialis vein. RBC count, hemoglobin level, and the hematocrit ??were measured using routine hematological examination by auto analyzer machine Scil Vet ABC (ABC Vet 16p). Data were then analyzed by one-way ANOVA. The results showed that the administration of water hyacinth (Eichhornia crassipes) collected from Lead (Pb) polluted water was not influence the RBC count, hemoglobin level, and the hematocrit in pig (p>0.05).
Perbedaan Heritabilitas Infeksi Heterakis gallinarum pada Ayam Lokal dan Ras Lohman I Made Angga Prayoga; Nyoman Adi Suratma; I Made Damriyasa
Buletin Veteriner Udayana Vol. 6 No. 2 Agustus 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The study was couducted to determine the difference of heritability of Heterakis gallinarum in local and Lohman chicken. Sixteen local chicken and 16 Lohman chicken were inoculated with infected egg of Heterakis gallinarum for 250 egg in ech chicken. All chiken were necropsed 3 months after inoculated. The heritabilty of the worm was evaluated by determinan the number of infected chicken and number of worms. There were found 10 local chicken infected by Heterakis gallinarum and 2 local chicken infected by the worm. The result of the study indicated that the heratibility of Heterakis gallinarum is  higher in local chicken than Lohman chicken.
Histopatologi Bronkiolus dan Pembuluh Darah Paru Mencit Jantan Pasca Terpapar Asap Rokok Elektrik Mia Monica; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.988 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p08

Abstract

This study aims to determine the histopathological changes of bronchioles and blood vessel of mice (Mus musculus) lung post exposed of electric cigarette smoke (vaping). This study was used 24 mice that were divided into two treatment groups. Group I (P0) as a control without electronic cigarette smoke exposured and group II (P1) consistied of 12 mice as a treatment group, were exprosure with electric cigarette smoke. This study used a complete randomized design (RAL) split in time pattern with three 1st, 2nd and 3rd post-treatment sampling times. Four mice of each group were taken at each observation time. Lung tissue was taken to make preparations and stained with Hematoxylin-eosin (HE) staining. The results showed that there were significant differences (P <0.05) between the treatment group and the control group, but the duration of exposure did not have an effect. Mucosal thickness in the treatment group was higher than in the control group (P <0.05). Vessel lung endothelins and thrombosis. It can be concluded, exposure to electric cigarette smoke can cause degeneration, cell necrosis and increase the mucosal thickness of the bronchioles. The vascular system experiences endotheliosis, endothelial cell necrosis and thrombosis
STUDI HISTOPATOLOGI HATI TIKUS PUTIH (Rattus norvegicus) YANG DIBERI EKSTRAK ETANOL DAUN KEDONDONG (Spondias dulcis G.Forst) SECARA ORAL I Putu Arya Adikara; Ida Bagus Oka Winaya; I Wayan Sudira
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Tanaman kedondong sering dimanfaatkan oleh masyarakat sebagai obat alternatif  untuk mengobati berbagai macam penyakit. Sedangkan penelitian tentang toksisitas daun kedondong pada hati belum pernah dilakukan. Dalam penelitian ini tikus putih (Rattus norvegicus) dibagi secara acak menjadi lima kelompok, masing-masing kelompok berjumlah 5 ekor. Kelompok A sebagai kontrol (placebo) yang diberi aquades peroral; kelompok B diberikan ekstrak daun kedondong 100 mg/kg bb (0,2 ml/ekor); kelompok C diberikan ekstrak daun kedondong 200 mg/kg bb (0,4 ml/ekor); kelompok D diberikan ekstrak daun kedondong 300 mg/kg bb (0,6 ml/ekor); kelompok E diberikan ekstrak daun kedondong 400 mg/kg bb (0,8 ml/ekor). Pemberian ekstrak daun kedondong dilakukan secara oral. Dilanjutkan dengan nekropsi pada hari ke-15 untuk pengambilan organ hati yang nantinya akan dibuat preparat hitopatologi.Hasil pemeriksaan histopatologi pada hati  tikus putih(Rattus norvegicus) yang diberikan ekstrak etanol daun kedondong, tidak ditemukan adanya perubahan seperti adanya infiltrasi sel radang, degenerasi melemak, degenerasi hidrofik dan nekrosis pada kelompok perlakuan K1, K2, K3, tetapididapatkan hasil peradangan ringan pada kelompok perlakuan K4 dan K5 pada 1 ekor tikus putih (Rattus norvegicus) dari 5 ekor yang diberi perlakuan pada tikus putih (Rattus norvegicus).
Karakteristik Fisikokimia dan Uji Aktivitas Antimikroba Bakteriosin dari Isolat Bakteri Asam Laktat 15B hasil Isolasi Kolon Sapi Bali Ni Kadek Lyming Lestari; I Wayan Suardana; I Dewa Made Sukrama
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.044 KB) | DOI: 10.24843/bulvet.2019.v11.i01.p11

Abstract

Lactic acid bacteria (LAB) is a group of Gram-positive bacteria, in shape of coccus or bacilli and catalase-negative and also have the ability to produce lactic acid. LAB is also a microorganism which is not toxic and has the ability to produce antimicrobial compounds, known as bacteriocins. This research aimed to find out the physicochemical characteristic and the antimicrobial activities of bacteriocin isolated from isolates 15B originated from the bali cattle’colon. This study began by reculturing of isolates 15B and followed by cultivating the isolates, isolating, producing, and precipitating of bacteriocins. The physical and chemical properties of the bacteriocins were also tested before, antimicrobial activity test was conducted. The result of the cultivation showed that isolates 15B is a lactic acid bacteria characterized by the result of Gram staining as a Gram positive, catalase negative and grown on anaerobic condition. The result of chemical test, showed that bacteriocins producing isolates 15B were positive on Ninhydrin test, negative on Molisch test, and positive on Lowry test with 0,11 µg/ml concentration. Physical properties test using SDS PAGE showed negative result and antimicrobial activity test showed that the bacteriocin from isolates 15B has 35,82% effectiveness of inhibition.
Total Dan Diferensial Leukosit Babi Landrace Yang Diberi Pakan Eceng Gondok (Eichornia crassipes) Dari Perairan Tercemar Timbal (Pb) Komang Suciani Paramita; I Gede Mahardika; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.326 KB)

Abstract

The aim of this study was to determine the effect of water hyacinth (Eichornia crassipes) collected from Lead (Pb) polluted water in feed total and differential leukocyte counts of pigs. This study was an experimental study and done used a completely randomized design. The sample that been used was blood from a total of eight Landrace swines, consisted of four groups: pigs were feed without hyacinth (A), pigs were feed with 2,5% hyacinth (B), pigs were feed with 5% hyacinth (C), and pigs were feed with 7,5% hyacinth (D). Blood were then collected from auricularis superficialis vein. The total and differential leukocytes were checked in the Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Udayana University. Total leukocytes count were measured by auto analyzer Scil Vet ABC (ABC Vet 16p). The blood were also processed for blood smear preparation by Giemsa staining before performing examination by using 100x magnificence under a microscope using straight edge method. Data were then analyzed by one-way ANOVA. The results showed that the administration of water hyacinth (Eichornia crassipes) collected from Lead (Pb) polluted water was not influence the total and differential leukocyte counts in pigs.
Hubungan Antara Dimensi Panjang Induk Dengan Pedet Pada Sapi Bali (CORELATION BETWEEN THE SIZE OF THE BODY PART OF DAM AND CALF IN BALI CATTLE) Ni Nyoman Janipa Saptayanti; I Ketut Suatha; I Putu Sampurna
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This research aimed to determine the relation between the size of the body part of dam and calf in bali cattle and to get cattle’s size influence calve’s size. Twenty cows and 20 calves each 10male calfes and 10 female new born calfes from Getasan village, District of Petang, BadungRegency. Data of the sizes were analyzed by linear regression. While the sex affect on body sizewas determine by T test. T test results indicated that sex of the calf did not significantly affect therelation between the size of the dams with the calves. But there is a relation between the size of the dams with the calves and there is a comparison the size of each dams with it calves. The mostaccurate relation starting from the size of the ear, neck size, size of head , tail size and size of theear
Pengaruh Pemberian Propolis Terhadap Gambaran Histopatologi Ginjal Tikus Putih yang Diberikan Parasetamol Dosis Tinggi Elti Febilani; I Ketut Berata; Samsuri Samsuri; I Made Merdana; Luh Made Sudimartini
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The purpose of this study is to determine the effect of propolis on kidney histopathological representation of white rats were given the toxic dose paracetamol. 25 male rats males were used in this study who were divided into 5 groups, control group (P0) without treatment, group 1 (P1) that was given paracetamol doses 250mg/kg, group 2 (P2), group 3 (P3), and group 4 (P4), given paracetamol dose of 250mg / kg and propolis added 0.25 ml (P2), 0.50 ml (P3), and 0.75 ml (P4). Paracetamol and propolis were given orally by mixing it into the water provided ad libitum for 10 days. Then performed a necropsy and kidney were taken aseptically for making of histopathology preparations with hematoxylin eosin (HE) staining. The examined variables were fatty degeneration and necrosis in the proximal tubules of the kidney. Results indicate that fatty degeneration and necrosis were showed in treatment P1, while the entire treatment P2, P3, and P4 show the repair from the damage caused by the paracetamol. Treatment P4 gives the best results in reducing the effects of the paracetamol. Kruskal-Wallis showed a significant difference from fatty degeneration and necrosis of the tested group. From this study it can be concluded that the propolis with dose 0.75 ml giving better protection than 0.25 ml and 0.50 ml dose.
Klasterisasi Manajemen Pengolahan Limbah Sapi Bali pada Simantri di Kabupaten Badung Dewa Made Dwi Parwata; I Putu Sampurna; I Made Sukada; Kadek Karang Agustina
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.878 KB) | DOI: 10.24843/bulvet.2019.v11.i01.p09

Abstract

This research aims were to find out the components of bali cattle waste processing that have not been intensively carried out and to find out which Gapoktan has not carried out an intensive Bali cattle processing system in Simantri Badung Regency. Sampling was done by saturated sampling technique, then the data obtained were analyzed using Hierarchical cluster with dendogram cluster plot with intensive, semi-intensive, and extensive identifying variables. The results of this study are the management components of waste processing which are mostly intensively treated by breeders, solid waste treatment, wastewater treatment, solid waste treatment technology, wastewater treatment technology, solid waste treatment equipment, liquid waste treatment equipment, waste management fostering, waste product marketing coaching. Onen housing management is still applied semi-intensively, namely the results of processing solid waste, the results of processing wastewater, the advantages of processing solid waste and the benefits of processing wastewater. The conclusion of this study is that from 50 Gapoktan in Simantri in Badung Regency, 42 Gapoktan already carry out intensive waste treatment, 6 Gapoktan that carry out semi-intensive waste treatment and 2 Gapoktan who do not carry out waste treatment or enter into an extensive category that needs to get counseling from the government so that everything becomes intensive.

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