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Pro-Life
Published by Universitas Kristen Indonesia
ISSN : 23020903     EISSN : 25797557     DOI : 10.33541
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Ilmu Biologi merupakan salah satu ilmu yang terus bertumbuh dan menjadi bagian yang penting dalam kehidupan. Ilmu Biologi mencakup kajian teori, penelitian hingga pendidikan Biologi menjadi cakupan ilmiah yang terus diteliti dan dikaji hingga memperoleh manfaat bagi kehidupan. Program studi pendidikan Biologi FKIP-UKI ingin memberikan wadah bagi para peneliti baik di dunia Ilmu Biologi maupun Pendidikan Biologi untuk mempublikasikan hasil kajiannya. Jurnal ini terbentuk pada tahun 2014 dan menjadi referensi bagi para mahasiswa dan peneliti lain dalam melakukan penelitian. Bidang spesifik yang dicakup dalam Jurnal adalah Pendidikan dan Ilmu Biologi. Cakupan/ fokus masalah yang dicakup dalam Jurnal antara lain Pendidikan, Metode Ajar, Tumbuhan, Hewan dan Lingkungan. Jurnal terbit 3 kali dalam satu tahun, yaitu pada Bulan November, Maret dan Juli.
Arjuna Subject : -
Articles 230 Documents
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Karakteristik Morfo-Anatomi serta Kandungan Klorofil dan Kandungan Fitokimia Tanaman Ketul (Bidens pilosa) pada Tempat dengan Intensitas Cahaya yang Berbeda di Arboretum Universitas Padjadjaran Marcelline, Karen; Nurzaman, Mohamad; Setiawati, Tia
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

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Abstract

Bidens pilosa is a plant known for its secondary metabolites with therapeutic properties. This study aimed to examine the effects of different light intensities on the morphology, anatomy, chlorophyll content, and secondary metabolite production of B. pilosa in the Arboretum of Universitas Padjadjaran. The shaded and unshaded area had light intensities of 5828 and 32768 lux, respectively. Leaf samples were collected from three different individuals in each area, with three leaves taken per plant. Morphological and anatomical traits such as leaf thickness, leaf area, stomatal density, and chlorophyll content were observed, along with secondary metabolite content. Results showed that plants in the shaded area had thinner leaves (0,19 mm) and lower stomatal density (275,16 cells/mm2) but a larger leaf area (4 cm2) and higher chlorophyll content (29,33 CCI) compared to leaves in the unshaded area, which had thicker leaves (0,213 mm), higher stomatal density (310,83 cells/mm2), a smaller leaf area (2,33 cm2), and lower chlorophyll content (27,27 CCI). Alkaloids, flavonoids, tannins, and saponins were detected in both conditions, with a higher level of alkaloid and tannin in unshaded plants. These findings enhance our understanding of the relationship between light intensity influences plant morphology, photosynthesis, and secondary metabolite production, with potential applications in cultivation and medicinal use.
Perbedaan Struktur Morfologi, Anatomi, dan Fisiologi serta Kandungan Fitokimia Daun Aur-Aur (Commelina diffusa) pada Intensitas Cahaya Berbeda di Embung Leuwi Padjadjaran Handayani, Seviana Esti; Hasan, Rusdi; Mutaqin, Asep Zainal
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

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Abstract

Commelina diffusa (family Commelinaceae) contains various secondary metabolites with potential antimicrobial and antioxidant properties. The production of these compounds is influenced by environmental factors, exceptionally light intensity, which also plays a role in determining the plant’s morphological and anatomical characteristics. This study aims to analyze differences in leaf morphology, stomatal anatomical structure, chlorophyll content, and phytochemical compounds in C. diffusa leaves growing in two locations with different light conditions at Embung Leuwi Padjadjaran, Sumedang. A descriptive method was used to examine leaf morphology, stomatal anatomy, chlorophyll content, and phytochemical compounds of C. diffusa in shaded and unshaded locations. The results showed that leaf morphology was relatively similar in both locations, but the leaf surface area was more significant in unshaded conditions (8,33 cm²). The stomata were hexacytic type with higher stomatal density in the shaded condition (591,082 cell/mm²) and a higher chlorophyll content (37,73 CCI). The result of Phytochemical analysis confirmed the presence of alkaloids, flavonoids, saponins, tannins, and quinones, which were relatively higher in unshaded conditions.
Enhancing Learning Motivation and Student Engagement through Virtual Reality in Virus Topic Learning Luther Jonathan Samori; Adisti Ratnapuri
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.6716

Abstract

The integration of innovative technologies into biology education has become increasingly important for enhancing students' motivation and engagement, particularly when studying abstract topics such as viruses. This study aimed to examine students' responses to the use of Virtual Reality (VR) in learning about viruses in biology. The study employed a Classroom Action Research (CAR) design based on the model of Kemmis and McTaggart and was conducted in two cycles. Data were collected through student questionnaires. The results showed that the average response in the first cycle reached 68.73%, which was categorized as sufficient. In the second cycle, involving 35 students, the average percentage increased to 81.79%, indicating a higher level of positive responses. More than 80% of students reported increased motivation to learn and greater active participation in discussions, group work, and interactions with learning materials through VR simulations. These findings suggest that VR-supported learning can enhance students' motivation and engagement in understanding complex biological concepts.
Synthesis of Literature on Antioxidant Activity and Safety of Seroja (Nelumbo nucifera Gaertn.) Plant Relative to Pharmacological Potential Kendrick Mahapurisa; Ferina Angelia; William
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.6939

Abstract

Nelumbo nucifera Gaertn. (Nelumbonaceae), known as the sacred lotus, is widely distributed across Asia and has long been used as food and traditional medicine, although its utilization in Indonesia remains largely ornamental. Interest in its antioxidant potential and its role as a source of bioactive compounds has increased. This review aims to systematically summarize in vitro and in vivo evidence on the antioxidant activity of N. nucifera, identify key plant parts and active compounds, compare extraction and assay methods, and evaluate preclinical bioactivity and safety. Literature searches in Google Scholar, NCBI, and PubMed using the keywords "Seroja" OR "Nelumbo nucifera" AND "antioxidant" yielded 27 eligible articles. Antioxidant activity is mainly attributed to flavonoids, alkaloids, and phenolic compounds, particularly quercetin and neferine. Flowers and seeds generally show higher in vitro antioxidant capacity, whereas leaves and rhizomes are more frequently studied in vivo. Variations in extraction and assay methods contribute to heterogeneous results. Preclinical data suggest a favorable acute safety profile; however, long-term toxicity data remain limited, and no clinical trials have been conducted. Overall, N. nucifera shows promising antioxidant potential, but further standardized toxicological studies and clinical trials are needed to support its therapeutic use.
Enhancing High School Students' Understanding of the Excretory System through Problem-Based Learning Dhea Febria; Aswinda Aswinda; Septia Yuliadip; Fulsiana Yustia; Kharisma Dwi Khorneliana; Jodion Siburian; Ine Tentia
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.7621

Abstract

Low cognitive learning outcomes in biology on the excretory system are often due to conventional learning methods that make students passive and focus on memorization. This study aims to improve students' cognitive learning outcomes by applying the Problem-Based Learning (PBL) model. The study used the Classroom Action Research (CAR) method, conducted in two cycles with 24 students from SMA Negeri 1 Kota Jambi. Data were collected through pretests and posttests in each cycle and analyzed using descriptive statistics, including average scores and learning completeness percentages. The results showed an increase in the average score from 46.25 in the pre-cycle to 80.83 in cycle I and 87.08 in cycle II, with the mastery percentage increasing from 4.17% to 100%. These findings indicate that the PBL model is effective in improving cognitive learning outcomes and fostering students' conceptual understanding and critical thinking skills. This study contributes by showing that the application of PBL through classroom action research can be an effective learning strategy for improving the quality of biology learning in secondary schools.
Quantitative Analysis of Phytochemicals and Antioxidant Activity in Vitro Ethanol Extract of Jengkol Fruit Peel (Archidendron pauciflorum (Benth.) I.C.Nielsen) Dinda Hani'ah Arum Saputri; Desak Made Malini; Nining Ratningsih
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.7835

Abstract

Jengkol (Archidendron pauciflorum (Benth.) I.C.Nielsen) is a widely consumed crop in Indonesia, while its fruit peel is generally considered an agricultural waste and underutilized. This study aimed to evaluate the phytochemical content and in vitro antioxidant activity of an ethanol extract of jengkol fruit peel. Phytochemical screening was performed to identify the presence of flavonoids, total polyphenols, and tannins, while antioxidant activity was assessed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. All experiments were conducted in duplicate, and the results were expressed as mean ± standard deviation. Data were analyzed using descriptive statistical analysis and linear regression to determine IC50 values. Phytochemical analysis showed that the extract contained flavonoids (0.23%), total polyphenols (28.82%), and tannins (3.83%). The ethanol extract of jengkol fruit peel showed very strong in vitro antioxidant activity, with an IC₅₀ value of 6.3 ppm. The high antioxidant activity is likely attributable to the presence of phenolic compounds, which contribute to free radical scavenging by donating electrons or hydrogen atoms. However, this study was limited to in vitro evaluation using a single antioxidant assay method. Further studies employing additional antioxidant assays and in vivo assays are needed to more comprehensively evaluate the potential of jengkol fruit peel extract as an antioxidant.
Beyond Discovery Learning: The Impact of the Mordiscvein Model on Students’ Critical Thinking Skills in Biology Raicha Oktafiani; Aryani Dwi Kesumawardani; Yoga Aditya Saputra; Muhammad Farhan Barata
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.7838

Abstract

Critical thinking skills are essential competencies for students in the 21st century; however, conventional instructional models such as discovery learning often fail to optimally foster students’ engagement in higher-order thinking processes. This study examined the effect of the Mordiscvein learning model on senior high school students' critical thinking skills in biology compared with the discovery learning model. A quasi-experimental design with a non-equivalent control group was employed involving 72 tenth-grade students (36 experimental, 36 control) at a public senior high school in Bandar Lampung, Indonesia. The intervention was conducted over four weeks (eight 90-minute sessions). Data were collected using a 10-item essay-based critical thinking test covering analysis, inference, and evaluation indicators, with acceptable reliability (Cronbach's α = 0.82). The results indicated that students taught using the Mordiscvein model achieved significantly higher posttest scores than those in the discovery learning group. Improvements were observed across all critical thinking indicators, suggesting that structured inquiry stages problem orientation, collaborative inquiry, guided experimentation, data interpretation, and reflective discussion—contribute to deeper cognitive engagement. These findings indicate that integrating systematic scaffolding into constructivist learning environments can more effectively support the development of critical thinking in biology education.
Tanggo-Tanggo: An Ethnobiological Study of a Traditional Batak Toba Food for Biodiversity Learning through Educational Videos Shilkia M., Daniel; Adinugraha, Fajar; Sunarto
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.7855

Abstract

Traditional foods are part of indigenous knowledge or local wisdom that reflects cultural identity, natural potential, and the social values of local communities. However, knowledge related to traditional foods is often transmitted orally from generation to generation and has not been well documented scientifically, particularly regarding the use of local biodiversity. Tanggo-tanggo is a traditional Batak food that represents the close relationship between culture and local biodiversity. This study aims to examine Tanggo-tanggo from an ethnobiological perspective and develop local culture-based learning media for formal education in schools, integrating it with community-based informal learning to support cultural conservation. The research was conducted using an ethnobiological approach, and data were collected through interviews with seven informants using snowball sampling and participatory observation. The process of making Tanggo-tanggo involves 16 species, comprising 15 plant species and 1 animal species, namely pigs (Sus scrofa domesticus), with plant parts used including rhizomes, fruits, flowers, tubers, and leaves. The processing techniques are simple and have been passed down from generation to generation as part of Batak community traditions. The results show that Tanggo-tanggo not only functions as a dish but also as an expression of cultural identity and rich local knowledge. This research is expected to contribute to ethnobiological documentation, support local culture-based education, and promote food security, natural resource conservation, and community empowerment.
Vegetative Growth Response of Robusta Coffee Seedlings (Coffea canephora Pierre ex A. Froehner) to Various Concentrations of Atonic Growth Regulators Novi Yulanda Sari; Nursamsi; Muhammad Syahfitra; Novfirman; Dyah Puspita Sari
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.7897

Abstract

Seedling quality is a critical determinant of the long-term productivity of robusta coffee. Under nursery conditions, seedlings often exhibit slow and uneven growth, which may limit canopy development and photosynthetic efficiency. Therefore, strategies that enhance physiological processes are needed to improve seedling performance. Atonik, a plant growth regulator (PGR) containing nitrophenolate compounds, has been reported to stimulate metabolic activity and enhance plant growth. However, information regarding the optimal concentration of Atonik for seed-propagated robusta coffee seedlings remains limited. This study aimed to evaluate the vegetative growth response of robusta coffee seedlings to different concentrations of Atonik and to determine the most effective rate during the nursery stage. The experiment was arranged in a completely randomized design (CRD) with a single factor consisting of five Atonik concentrations (0, 10, 15, 20, and 25 ml/L) and three replications. Observed variables included plant height, leaf number, stem diameter, leaf length, leaf width, total fresh weight, and total dry weight. Data were analyzed using ANOVA followed by Duncan’s Multiple Range Test at 5%. The results showed that Atonik application significantly increased plant height, stem diameter, leaf width, total fresh weight, and total dry weight. The 15 ml/L concentration significantly increased plant height and leaf width, whereas the 20 ml/L concentration significantly improved stem diameter and biomass accumulation. Overall, the 20 ml/L treatment was the most effective in enhancing seedling vigor during the nursery stage.
Item Analysis of Creative Thinking Skills Using Rasch Modelling in the Ethnozoology of Traditional Foods Alif Yanuar Zukmadini; Kasrina Kasrina; Abdul Rahman Singkam; Abas Abas; Ayu Sulistiawati
Pro-Life Vol. 13 No. 1 (2026): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v13i1.7899

Abstract

Prospective biology teachers need creative thinking skills to address biodiversity issues, such as traditional food practices. Measuring these skills requires valid, reliable, and appropriate instruments. This study evaluated a creative thinking skills instrument in traditional food ethnozoology using the Rasch model. Employing a quantitative descriptive method, the instrument contains 15 multiple-choice questions based on six creative thinking indicators: curiosity, fluency, originality, elaboration, imagination, and flexibility. A pilot test involved 45 prospective biology teachers. Data were analyzed with the Winsteps application to assess item difficulty, item fit, bias detection (DIF), and reliability. Results showed item difficulty ranged from ±3.69 logits, with two items rated as difficult and three as easy. Thirteen items fit well, one was borderline, and one required revision. DIF analysis showed no significant gender bias. Item reliability was 0.86 (good), person reliability was 0.63 (poor), and Cronbach's alpha was 0.71 (fair). The instrument meets validity and reliability criteria, but adding more difficult items may improve its discrimination among high-ability respondents. Thus, the instrument can be used to diagnose creative thinking skills specifically related to traditional food ethnozoology in prospective biology teachers, helping to identify areas for further development.

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