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BERITA BIOLOGI
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Articles 15 Documents
Search results for , issue " Vol 10, No 6 (2011)" : 15 Documents clear
POLA KECENDERUNGAN PENANGKAPAN BURUNG-BURUNG LIAR BERNILAI EKONOMIS DAN IMPLIKASI KONSERVASINYA: STUDI KASUS DITANAH GROGOT, KABUPATEN PASER, PROVINSI KALIMANTAN TIMUR Suba, Rachmat Budiwijaya; Rakhman, Aditya; Rustam, Rustam
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (927.936 KB) | DOI: 10.14203/beritabiologi.v10i6.1949

Abstract

The study aims at quantifying trade scale of some economically wild birds and describing captured trends which have been practised by wild bird catchers. The study was conducted in Tanah Grogot, Paser District, East Kalimantan Province, from September to December 2008, by surveying wild bird market and interviewing catchers and sellers. We measured two main attributes of market dynamics; first, each species availability in the market as the percentage of market days sampled where the species appeared; second, daily abundance of a species as the monthly average number of each bird per day for all days sampled. Seven wild bird species that have been traded commodities for years were White-rumped Shama Copsychus malabaricus (25.41%; 602.25+70.79 individuals per month), Yellow-vented Bulbul Pycnonotus goiavier (11.47%; 111.25+12.50 individuals per month), Crested Myna Acridotheres cristatellus (12.30%; 73.25+10.44 individuals per month), Spotted Dove Streptopelia chinensis (5.73%; 22.00+12.83 individuals per month), Hill Myna Gracula religiosa (12.30%;12.00+0.82 individuals per month), Greater Green Leafbird Chloropsis sonneratii (7.38%; 11.75+2.50 individuals per month) and Magpie Robin Copsychus saularis (6.56%; 8.75+1.71 individualsper month). Specific capture location within Paser District could reflect distribution and habitat preference of each bird species.
KORELASIANTAR-KARAKTER DAN SIDIK LINTAS ANTARA KARAKTER AGRONOMIDENGAN HASIL KEDELAI {Glycine max (L.) Merrill} Hakim, Lukman
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.063 KB) | DOI: 10.14203/beritabiologi.v10i6.1936

Abstract

A total of 27 soybean {Glycine max (L.) Merrill} genotypes including check variety (Kawi) were evaluated on dry land at Ngale Experimental Farm, Ngawi, East Java during early rainy season of 2009/2010. The experiment was conducted in a randomized complete block design with three replications. Plot size was 4 m x 5 m, plant spacing was 40cmx20cm, each hill contained two plants. A correlation and path coefficient analyses were used in order to determine the association among characters and quantify the direct and indirect effects of agronomic traits on seed yield. The study has identified 3 varieties, i.e. Kaba, Wilis and Tanggamus produced the highest yield (>1.6 t/ha) and had good adaptation on dry land. Two varieties (Grobogan and Argomulyo) having large seed size (>15 g/100 seeds), and four varieties,i.e. Tidar, Grobogan, Gepak Ijo and SHR-W60 had early maturity (81days). Therefore, these varieties can be used as source of parents on breeding program. Among the agronomic characters, plant height, number of pods per plant and harvest index were positively and significantly correlated with grain yield. The direct effect of plant height, number of pods per plant and harvest index to grain yield as indicated by path coefficient were the highest, while other causal effects to grain yield were small or negative. Based on both analyses, soybean genotypes with high grain yield should have sufficient plant height, high number of pods per plant and high harvest index. Therefore, plant height, number of pods per plant and harvest index can be suggested for selection criteria in selecting high yield soybean genotypes on dry land.
SIFAT PROTEKSI EKSTRAK AIR PANAS TEH {Camellia sinensis (L.) Kuntze} HIJAU PADA KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARACETAMOL Julistiono, Heddy
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.535 KB) | DOI: 10.14203/beritabiologi.v10i6.1943

Abstract

In order to develop yeast Candida tropicalis as a model cell for evaluation of substance having anti- or pro-oxidant activity in cell level, the effect of hot water tea {Camellia sinensis (LJ Kuntze) extract on peroxidized lipids, a marker of oxidative stress and cell mortality in the yeast caused by paracetamol has been evaluated. Incubation of yeast cell in the presence of 1.38 % green tea for 2 h decreased cell viability followed with increasing of peroxidized lipids, whereas 0.27 % green tea caused increasing of peroxidized lipids without causing cell death.Yeast cell was not affected by 0.1 % green tea. Incubation of yeast cell with presence of 0.15 % (g/v) paracetamol for 2 h did not cause cell mortality, however content of peroxidized lipids increased significantly. In the presence of higher dose of paracetamol (0.3 %) cell viability remarkably decreased and followed with increasing of peroxidized lipids significantly. Green tea of 0.1 % increased cell viability of cells treated with 0.3 % paracetamol while peroxidised lipids decreased. The data indicated that high dose of paracetamol caused oxidative stress in yeast cells, while green tea with lower concentration might protect paracetamol toxicity due to its antioxidant property. Although the antioxidant property, high dose of green tea could cause oxidation due to its pro-oxidant activity. In conclusion, yeast C. tropicalis could be potentially used as a model cell to evaluate substances having antioxidant property in cell level.
IDENTIFIKASI Lernaea sp. YANG MENGINFEKSI IKAN ARWANA IRIAN {{Scleropagesjardinii (Saville-Kent, 1892)} DI MERAUKE, JAKARTA, BOGOR DAN DEPOK Shatrie, Dikry N; Imamudin, Kurniasih; Nurcahyo, Wisnu; Triyanto, Triyanto
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1085.437 KB) | DOI: 10.14203/bb.v10i6.1950

Abstract

Lemaea spp. is very important fish ectoparasites because it spreads all over the world and its economic importance has increased due to numerous epizootics occurrence among the most important farmed fish; and has wide host range. Lemaea sp. has been infected not only the family Cyprinidae and Characidae but also in Osteoglossidae family, such as Red Arwana [Scleropagesformosus) in Malaysia and Arwana fish {Scleropagesjardinii (Saville-Kent, 1892)) in Irian.The objective of this study was to identity Lernaea sp. often attacked Arwana irian fish as a precautionary measure the spread of infection, especially on Arwana irian fish. Arwana irian fish were collected from Merauke, Jakarta, Bogor and Depok, 10 infected fish per area. Found Lernaea sp. fixed in glutaraldehyde for morphology study. Based on morphological charactheristic, there were six different Lemaea sp. attacked Arwana irian fish. They are Lemaea cyprinacea, L. devastatrix, L. lophiara, L.oryzophila, L. papuensis and Lemaea n sp. Lemaea n sp. was considered as a new species due to different variation of holdfast organ.
HIDROLISIS KITIN MELALUI FERMENTASI SEMI PADAT UNTUK PRODUKSI N-ASETILGLUKOSAMINA Saskiawan, Iwan; Handayani, Rini
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.249 KB) | DOI: 10.14203/beritabiologi.v10i6.1937

Abstract

N-acetyl-D-glucosamine (GlcNAc), the monomeric unit of polymer chitin has attracted much attention for their therapeutic activity in osteoarthritis.It is mainly produced by acid hydrolysis of chitin which affect the environmental problem because of its acidic wastes.Therefore, it is need to develop the new method for GlcNAc production. The aim of this experiment is to produce GlcNAc by mean of submerged fermentation of chitin. The preliminary study showed that fermentation of chitin by Aspergillus sp.501 produced higher GlcNAc than that of Saccharomyces sp. It was 1.39 ng/ml and 1.07 ng/ml. Then the Aspergillus sp. 501 was used in optimation of GlcNAc production. The effect of pH and nitrogen course such as bacto peptone, yeast extract, amonium sulfat and urea to GlcNAc production was examined. Then the product of GlcNAc was precipited by vacum evapotated and freeze dryed. The results showed that the highest of production GlcNAc of 2.228 ng/mL was obtained on pH 4 of medium solid state fermentation using urea as Nitrogen source at 10 days incubation.
INFEKSI Salmonella enteritidis PADA TELUR AYAM DAN MANUSIA SERTA RESISTENSINYA TERHADAP ANTIMIKROBA Kusumaningsih, Anni; Sudarwanto, M
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.044 KB) | DOI: 10.14203/beritabiologi.v10i6.1944

Abstract

Salmonella enteritidis is one of pathogenic bacteria in chicken and human with re-emerging foodbome pathogen. Uncontrol use of antimicrobial drugs for the prevention and treatment of infectious diseases in chicken and human may lead to the development of antimicrobial resistance. Sampels of chicken eggs were collected from markets, layer farms, and grant parent stock farms. Sampels from human were anal swabs collected from hospitals and a laboratory of microbiology. Identification of bacteria was conducted by isolation and biotyping using selected media for Salmonella, while serotyping S. enteritidis was conducted with spesific 0 somatic (1, 9, and 12) and H flageIJa (m) antigen.Antimicrobial resistance tests were conducted by the standard diffusion method using antimicrobial disks.The results of isolation and identification of Salmonella show that 9 out of 122 (7.4%) sampels of layer eggs and 7 out of 23 (30.4%) sampels of embrioneted eggs were positive with Salmonella spp. The results of serotyping against S. enteritidis show that 7 out of 9 (77.0%) from layer eggs and 7 out of 7 (100.0%) from embrioneted eggs were positive with S. enteritidis.From the IS anal swabs of human were obtained 14 (93,3%) contain S. enteritidis.The antimicrobial resistance profiles of S. enteritidis isolated from layer eggs show high resistances to streptomycin (42.9%), neomycin (85.7%), doxicycline (64.3%), and ciprofloxacin (57,1%), whereas those S. enteritidis isolated from human show high resistances to streptomycin (50.0%), neomicyn (85.7%), tetracycline (42.9%), and doxicycline (42.9%).Multiple resistance profiles of S. enteritidis isolated from eggs were mostly to 2-3 antibiotics and those of S. enteritidis isolated from human were mostly to more than 5 antibiotics.
KERAGAMAN GENETIK HlBRIDA BEBERAPA STRAIN IKAN NILA {Oreochromis niloticus Bleeker) Gustiano, Rudhy; Soelistyowati, Dinar; Fauzan, Agung Luthfi; Arifin, Otong Zenal
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.839 KB) | DOI: 10.14203/beritabiologi.v10i6.1951

Abstract

Objectives of this study are to determine the genetic variability among progeny of intraspecific crossing between three strains of Oreochromis niloticus Bleeker (BEST, Nirwana, and Red NIFI) using Randomly Amplified Polymorphic DNA (RAPD) and truss morphometric. The results showed that hybridization enabled to increase genetic variability respectedly on progeny of Nirwana male crossed to BEST female (27,93%) as well as to Red NIFI female. An analysist of truss morphometric indicated that BEST population had the lowest similarity and largest genetic distance (0,6) to the Nirwana and Red NIFI which were closer to the hybrid populations.
REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Sulistyaningsih, Yohana C; Hartana, Alex; Widyastuti, Utut; Hamim, Hamim; Suharsono, Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.374 KB) | DOI: 10.14203/beritabiologi.v10i6.1940

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.
IDENTIFIKASI GEN PENYANDI PIREN DIOKSIGENASE PADA ISOLAT BAKTERI PENDEGRADASI PIREN Febria, FA; Jamsari, Jamsari; Nasir, N; Nurhidayat, N
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.231 KB) | DOI: 10.14203/beritabiologi.v10i6.1946

Abstract

The pyrene dioxygenase coded by gene is an indicator of bacterial isolates capabilities in pyrene degradation. The encoded gene of pyrene dioxygenase can be amplified and detected in Pseudomonas sp. PyrA2 and Burkholdoriasp. PyrA4 isolates, using primary specific Diox which designed based on PhdF gene sequence, the coding gene of pyrene dioxygenase in Mycobacterium vanbalenii PYR-1. The sequence alignment of pyrene dioxygenase putative gene in both of bacterial isolates with the sequence of pyrene dioxygenase coding gene in M. vanbalenii PYR-1 shows the similarity percentage of 41 % and 42% with Pseudomonas sp. PyrA2 and Burkholdoria sp.PyRA4.
SIMTOMATOLOGI DAN WAKTU KEMATIAN RAYAP Macrotermes gilvus Hagen (ISOPTERA: FAMILI TERMITIDAE) SETELAHINFEKSI CENDAWAN Metarhizium brunneum Petch Sayuthi, Muhammad; Santoso, Teguh; Harahap, Idham Sakti; Kastosuwondo, Utomo
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.801 KB) | DOI: 10.14203/beritabiologi.v10i6.1941

Abstract

The potential of entomopathogenic fungus Metarhizium brunneum Petch as biocontrol agent to termite Macrotermes gilvus Hagen has been tested in the laboratory.The purpose of this research is to study the symptomatology and lethal time of the termite M. gilvus fungus after infection by M. brunneum. The density of conidia at 1.21xlO6/mL showed as effective concentration in causing M. gilvus mortality, when compared to 1.08><106conidia/mL, and control. The infection stage of M. brunneum on the host until the death of its host occurred on day 2,and the sporulation of M. brunneum on the surface of the host integument occur on day 4. The entire surface of M. gilvus was covered by the mycelium and conidia of M. brunneum on day 7. Lethal time 50% of termite population of M. gilvus (LT50) was achieved in 5 days(5.14), and LT95 achieved on day 10 (10.03).

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