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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Natur Indonesia Indonesian Journal of Agricultural Science Proceeding International Conference on Global Resource Conservation REINWARDTIA BERITA BIOLOGI Biosaintifika: Journal of Biology & Biology Education Makara Journal of Science Dinamika Kerajinan dan Batik: Majalah Ilmiah

ALEX HARTANA

Departemen Biologi, FMIPA Institut Pertanian Bogor, Kampus Darmaga Bogor

Author-ID : 276672

Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Social Sciences Other

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THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE Satoto, Satoto; Sulistyowati, Yuli; Hartana, Alex; -Loedin, Inez H. Slamet
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. DataÂ resulted from PCR of F2 population were analysed using a Chi Square test.The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of geneticsources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer orÂ separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.

THE ECOLOGY AND DISTRIBUTION OF FREYCINETIA GAUD. (PANDANACEAE; FREYCINETOIDEAE) IN THE INDONESIAN NEW GUINEA SINAGA, NURHAIDAH IRIANY; MEGIA, RITA; HARTANA, ALEX; KEIM, ARY PRIHARDHYANTO
REINWARDTIA Vol 13, No 2 (2010): Vol. 13 No. 2
Publisher : Research Center for Biology

SINAGA, N. I., MEGIA, R., HARTANA, A., KEIM, A. P. Ecology and distribution of Freycinetia Gaud. (Pandanaceae; Freycinetoidea) in Indonesian New Guinea. Reinwardtia 13(2): 189-197. - The study mainly concerns with the species of Freycinetia that occurs in the Indonesian New Guinea, including the provinces of Papua and Papua Barat. The study indicates that almost all species of Freycinetia in the Indonesian New Guinea prefer high humidity and abundantly occur along rivers, except for the members of the group of species with imbricate leaves, which inhabit also secondary forests. Futhermore, the members of this group have never been found within the range of 1700 to 3000 m altitudes. This highest range of altitudes is specifically occupied by the members of the groups of species with semi imbricate and grass-like leaves. The costal forests are inhabited by the groups of species with semi and non imbricate leaves. Indonesian New Guinea shares many species with Papua New Guinea, except for the members of the group of species with semi imbricate leaves, which are more common in Indonesian New Guinea than in Papua New Guinea. On the contrary, the members of the group of species with grass-like leaves are more common in Papua New Guinea and becoming rare toward the Indonesian site and becoming absent in the Vogelklop (Birds head), except for F. polyclada which is commonly found in Sorong. Indonesian New Guinea possesses 34 species exclusively distributed in the area, while Papua New Guinea has 72 species. The two areas share 52 species. Only five species have extra New Guinean distributions, i.e. F. excelsa, F. funicularis, F. marginata, F. percostata, and F. scandens.

Morphology vs. taxonomy in the family Pandanaceae: a case study in the Javanese species Rahayu, Sri Endarti; Chikmawati, Tatik; Kartawinata, Kuswata; Hartana, Alex
REINWARDTIA Vol 13, No 4 (2012): Vol. 13 no. 4
Publisher : Research Center for Biology

Since a large number of characters are now known for Freycinetia Gaudich. and Pandanus Parkinson species, it appears useful to consider their use in identifying plants from Java. Fieldwork carried out for this study has provided stronger foundation for understanding morphological variation within the species. This study was undertaken to have a better understanding on the morphology of the family in order to make a better species delimitation. Characters of habit, stem, leaves, auricles, bracts, peduncle and pedicel, inflorescence, staminate flowers (male), pistillate flowers (female), cephalia and berries were found useful in delimitation and identification of Javanese Freycinetia, while characters of habit, stem, prop root, marginal spine, leaves, bracts, inflorescentia, peduncle, staminate flowers (male), pistillate flowers (female), cephalia, drupes were found to be useful for distinguishing among species of Javanese Pandanus.Â

Leaf anatomy of Pandanus species (Pandanaceae) from Java Rahayu, Sri Endarti; Kartawinata, Kuswata; Chikmawati, Tatiek; Hartana, Alex
REINWARDTIA Vol 13, No 3 (2012): Vol. 13 No. 3
Publisher : Research Center for Biology

The leaf epidermis and mesophyll of fifteen species of Pandanus from Java were investigated to assess the value of anatomical features in species identification and classification. Characters of diagnostic importance are epidermal cell shape, differentiation of the abaxial epidermis into costa and intercosta, adaxial anticlinal cell wall outline, occurrence of raphides in the mesophyll, distribution of cubical crystals, pallisade cell shape, papillae on epidermal cells, and the stomatal complex. Leaf epidermal anatomy was found to be useful in the identification at species level.

Morphological Variation of Durio kutejensis (Hassk.) Priyanti, Priyanti; Chikmawati, Tatik; Sobir, Sobir; A. Rifai, Mien; Hartana, Alex
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Durio kutejensis (Hassk.) Becc. (lai) is a cultivated plant in Genus Durio except D. zibethinus Murr. (Durian). D. kutejensis is superior than D. zibethinus based on blunt spine, not fragrant, flashy color (dark yellow to orange) and storage ability up to 7-8 days. The aim of this research was to analyze the variation of morphological characters of 31 landrace trees which was collected from Batuah Village, Tanah Merah Village and Sebulu Village in East Kalimantan. Lai varied in several characters which were their crown shape, branch type, leaf shape, flower color, and fruit shape. The crown shape is pyramidal, semi circular, oblong, and umbrella. The branch types are spreading or erect to rather spread. The leaf shape is oblong, lanceolate or elliptic. The flower has pink or dark red color. The shape of fruit is globose, globose with 5-lobed, ovoid with 5-lobed, and oblong with 5-lobed.Â Â Key words: Durio kutejensis, morphology variation, superiorÂ Â

REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Sulistyaningsih, Yohana C; Hartana, Alex; Widyastuti, Utut; Hamim, Hamim; Suharsono, Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.

Characterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic Markers Manggabarani, Andi Madihah; Chikmawati, Tatik; Hartana, Alex
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12221

Rambutan (Nephelium lappaceum) is an economically important plant which is native to Indonesia and Malaysia. The diversity of rambutan in Indonesia is abundance especially in Kalimantan where the wild relatives still grow naturally. Rambutan cultivars are usually differed from each other based on fruit morphological characters. However, rambutan tree begins to fruiting for the first time in 3-4 years. Therefore, another character is needed to characterize each cultivar in a short period. The objectives of this study were to distinguish rambutan cultivars using leaf morphological and Inter-Simple Sequence Repeat (ISSR). As many as 30 rambutan cultivars collected from Cipaku Orchard and Mekar Sari Park were observed for their morphological and ISSR characters. Six characters of were surveyed for leaf morphological character. For the genetic character, 6 out of 31 ISSR primers were assessed which resulted in 58 polymorphic bands (87%). As a result, leaf morphological characters overlapped among cultivars causing difficulties distinguishing each cultivar. ISSR marker, three major clusters have been identified according to UPGMA method. Index similarity among rambutan accessions from ISSR data ranged from 48-93%. As a conclusion, ISSR marker could be potentially applied rambutan cultivars characterization.

Karakter Root Re-Growth Sebagai Parameter Toleransi Aluminium pada Tanaman Padi Roslim, Dewi Indriyani; Miftahudin, Miftahudin; Suharsono, Utut; Aswidinnoor, Hajrial; Hartana, Alex
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Aluminum (Al) is one of the major limited factors in crop production on acid soils. Aluminum tolerant plants can beselected from plant breeding program by one of the physiological parameters representing Al tolerance character,such as root re-growth capability during recovery from the Al-stress. In this study we determined the concentrationand time exposure of Al stress that was able to differentiate the response of three local upland rice varieties(Grogol, Hawarabunar and Krowal) and an Al-sensitive rice variety (IR64) to Al-stress, and evaluated the effectivenessof root re-growth (RRG) characters as an Al tolerance parameter in rice. The study consisted of three experiments,which were 1) nutrient culture experiment with different Al concentration treatments in growth chamber, 2) potexperiment in greenhouse using Jasinga yellow red podzolic acid soil containing 26,66 me/100 g Al and pH 4,6 asplanting media, and 3) phenotyping of F2 population using RRG character. The results showed that Al treatment at15 ppm for 72 h was able to distinctly differentiate between Al-tolerant (Grogol and Hawarabunar) and Al-sensitivevarieties (Krowal and IR64). Planting of the rice varieties on acid soils showed similar result as that of the nutrientculture. Phenotyping of F2 population using RRG character indicated the existence of RRG value variation. Thesevariations demonstrated that RRG character can be used as an Al tolerance parameter in rice and therefore can beeffectively applied to screen rice F2 population that segregate to Al tolerance character.

Craniofacial Shape of Arfak People Based on Geometric Morphometric Features Kawulur, Elda Irma Jeanne Joice; Suryobroto, Bambang; Budiarti, Sri; Hartana, Alex
Makara Journal of Science Vol. 22, No. 1
Publisher : UI Scholars Hub

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Face and cranial (craniofacial) shape is highly specific to the individual; therefore, craniofacial shape is often used to identify individuals and to analyze variability in the human population. Previous studies, consisting only of verbal descriptions, suggested that the cranial shape of the Papuan people was highly variable. Despite their usefulness, verbal descriptions cannot fully demonstrate common and local variation in cranial shape. They also cannot be used to extractthe general trend of variation or to group face shapes based on their similarity. Here we attempt to apply geometric analysis, a method of shape analysis, to measure facial anatomical structural landmarks of Papuan people. The craniofacial shape of Papuan people was constructed from those of Arfak people based on 16 anatomic landmarks on the lateral side. Arfak is one of the traditional Papuan tribes in Manokwari, West Papua Province. Our result showed great variation in craniofacial shapes among the Arfak. The nose, chin, and mandible differed significantly, whereas other parts of the face were relatively stable and showed small variations. These differences reflected variations in the facial growth rate. The high level of diversity thus indicates that some parts of the face have higher plasticity in their growth pattern than others.

Leaf Trichome Morphology of Durio Kutejensis Landraces from Kalimantan Priyanti,; Chikmawati, Tatik; Sobir,; Hartana, Alex
Makara Journal of Science Vol. 19, No. 1
Publisher : UI Scholars Hub

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The leaf trichome morphology of 19 Durio kutejensis (lai) landraces was studied. The observation of cross- and paradermal sections of D. kutejensis leaves showed that all landraces have glabrous leaves on the adaxial surface, while their abaxial surfaces are covered by six trichome types, one glandular (one- or two-celled stalks with a spheroid multicellular glandular head) and five non-glandular (complex peltate, simple peltate, cushioned stellate, flat stellate, and four-armed stellate trichome with a central cushion). All landraces were rarely covered by glandular trichomes. The non-glandular trichomes are varied in type, density, number of layers, diameter, and shape and margin color of the complex peltate among landraces. One landrace comprises three non-glandular trichome types, while the other landraces consist of four or five non-glandular trichome types. The shapes and margin color of complex peltate trichomes of D. kutejensis are the specific characteristics which distinguish this species from the other Durio species, however these characteristics cannot be used to differentiate one D. kutejensis landraces from the other. Therefore, other characteristics need to be explored in order to distinguish one D. kutejensis landraces from the other.

Co-Authors Ary Prihardhyanto Keim, Ary Prihardhyanto Azizah, Enur Bambang S. Purwoko Bambang Suryobroto Dewi Indriyani Roslim Dorly, Elda Irma Jeanne Joice Kawulur Fitmawati Fitmawati HAJRIAL ASWIDINNOOR Hamim Hamim Inez H. Slamet -Loedin KINYA AKASHI Kuswata Kartawinata Manggabarani, Andi Madihah Mien A. Rifai Miftahudin . Nampiah Sukarno Nina Ratna Djuita Nurhaidah Iriany Sinaga, Nurhaidah Iriany Priyanti Priyanti Priyanti, RITA MEGIA Saleha Hannum Satoto Satoto Sobir Sobir Sobir, Sri Budiarti Poerwanto Sri Endarti Rahayu, Sri Endarti Suharsono Suharsono Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Tatiek Chikmawati, Tatiek TATIK CHIKMAWATI Utut Suharsono UTUT WIDYASTUTI Utut Widyastuti Suharsono Yuli Sulistyowati

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