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All Journal Jurnal Sain Veteriner BERITA BIOLOGI Jurnal Medik Veteriner Jurnal Pengembangan Penyuluhan Pertanian Jurnal Kedokteran Hewan
Wisnu Nurcahyo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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IDENTIFIKASI Lernaea sp. YANG MENGINFEKSI IKAN ARWANA IRIAN {{Scleropagesjardinii (Saville-Kent, 1892)} DI MERAUKE, JAKARTA, BOGOR DAN DEPOK Shatrie, Dikry N; Imamudin, Kurniasih; Nurcahyo, Wisnu; Triyanto, Triyanto
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1085.437 KB) | DOI: 10.14203/bb.v10i6.1950

Abstract

Lemaea spp. is very important fish ectoparasites because it spreads all over the world and its economic importance has increased due to numerous epizootics occurrence among the most important farmed fish; and has wide host range. Lemaea sp. has been infected not only the family Cyprinidae and Characidae but also in Osteoglossidae family, such as Red Arwana [Scleropagesformosus) in Malaysia and Arwana fish {Scleropagesjardinii (Saville-Kent, 1892)) in Irian.The objective of this study was to identity Lernaea sp. often attacked Arwana irian fish as a precautionary measure the spread of infection, especially on Arwana irian fish. Arwana irian fish were collected from Merauke, Jakarta, Bogor and Depok, 10 infected fish per area. Found Lernaea sp. fixed in glutaraldehyde for morphology study. Based on morphological charactheristic, there were six different Lemaea sp. attacked Arwana irian fish. They are Lemaea cyprinacea, L. devastatrix, L. lophiara, L.oryzophila, L. papuensis and Lemaea n sp. Lemaea n sp. was considered as a new species due to different variation of holdfast organ.
POTENSI DAUN BAMBU SEBAGAI AGEN ANTHELMETIKA PADA TERNAK KAMBING ( Bamboo Leaves Potency as anthelmintic Agent on Goat) Widiarso, Widiarso, B. P.; Nurcahyo, Wisnu; Prastowo, Joko; Kurniasih, Kurniasih
Jurnal Pengembangan Penyuluhan Pertanian Vol 14, No 1 (2017): Jurnal Pengembangan Penyuluhan Peternakan
Publisher : UPPM Politekik Pembangunan Pertanian Yogyakarta Magelang (Polbangtan Yoma)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1305.029 KB)

Abstract

Daun bambu telah digunakan secara luas sebagai pakan alternatif pakan ternak ruminansia, namun dalam penggunaannya di lapangan, belum banyak dikaji manfaat lain selain sebagai sumber pakan. Kandungan tannin dalam daun bambu memberikan potensidaun bambu sebagai agen antelmetika. Selain mengandung kandungan nutrisi daun bambu: berat kering 91,27%; protein kasar 4,24%; lemak kasar 8,11%; serat kasar 27,2%; total digesti nutrien 36,42% .Daun bambu (Dendrocalamus strictus) setiap 100 mg mengandung Protein Kasar 15,09; Serat Kasar, 23,15; Lemak Kasar 1,43; Abu 18,03; Fosfor 170; Kalsium, 1550 mg (Attayaya, 2009). Tanin dalam daun bambu apus (Gigantochloa apus) tua 8,81% b/b, tanin dalam daun bambu petung (Dendrocalamus asper) tua 4,84% b/b, dan tanin dalam daun bambu legi (Gigantochloa atter ) tua 3,19% b/b. Hasil pengujiankandungan tanin di atas dapat menunjukkan bahwa daun bambu mepunyai potensi sebagai anthelmetika melawan cacing gastrointestinal. Tanin yang terdapat pada daun bambu adalah tanin terkondensasi. Tanin terkondensasi efektif melawan parasit GI. Efek tanin terkondensasi melawan parasit GI dilakukan baik secara langsung, yaitu melalui interakasi TK-nematoda, mempengaruhi penetasan dan mempengaruhi pertumbuhan larva infektif, maupun secara tidak langsung, yaitu dengan cara mengikat protein tumbuhan di dalamrumen sehingga mencegah degradasi mikrobial sehingga meningkatkan aliran protein ke duodenum yang pada akhirnya akan meningkatkan imunitas hospesKata Kunci: Antelmetika, Daun bambu, Kambing
MONITORING OF PHYSIOLOGICAL AND PARASITES STATUS OF BAWEAN DEER (AXIS KUHLII) IN ITS HABITAT AS A BASELINE FOR WILDLIFE CONSERVATION ENDEAVOR Nurcahyo, Wisnu; Anggraeni, Devita; Imron, M.A.
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.17882

Abstract

AbstractThe research on physiological and reproduction status of Bawean deer (Axis kuhlii) in its habitat has been conducted, to understand and to find out as a basic information on Bawean deer (Axis kuhlii) in its habitats a baseline data for wildlife conservation efforts. The deer is categorized as an endangered animal, therefore, more attention was given toward Bawean deer conservation. Habitat changes, loss of habitat, fragmentation and illegal hunting might caused the wild animals become more marginalized and the populations have been declined.Therefore, it is needed to have research as an effort to save Bawean deer in its natural habitat. This research activity covered monitoring on physiological and reproduction status, and also the examination of deer's feces samples was parasite coprological data that serve as a basic consideration data in conservation and determination of Bawean deers health in the nature and/or conservation. The research was done in Bawean Island, Gresik and East Java, by taking faces of Bawean deers in the nature and conservation. Data gathering of physiological andreproduction status were done by examining deer's condition, either directly in conservation or in its habitat, and also by interviewing people around the forest. The data on physiological status, behavior monitoring, reproduction status, faces samples, blood, food analysis, and interview result were analyzed descriptively. Some worm eggs were found during feces examination, those were Strongyl, Strongyloides sp, Trichuris sp, Fasciola gigantica and Oocysta coccidia. The result showed that the same parasites were found in the cattle and goat raised by people around in the vicinity of the forest, meaning, there was an interaction between wildlife andlivestock. According to the result, it is needed to monitor the physiological status of Bawean deers routinely since Bawean deers is classified as endangered species.
Investigation of Trypanosoma evansi in Sumatran Elephant (Elephas maximus sumatranus) in Indonesia Using Various Methods Purnamasari, Kartika; Nurcahyo, Wisnu; Nursalim, Muhammad Tauhid; Suwanti, Lucia Tri; Estoepangestie, Agnes Theresia Soelih
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.155-162

Abstract

Trypanosoma evansi is a widespread hemoprotozoa that causes trypanosomiasis in both livestock and wildlife. One of the susceptible animals is the Sumatran elephant, which is included in the endangered species category. The infection of this parasite often develops into chronic and sub-clinical forms in elephants, therefore it may become unnoticed and hard to diagnose. This study aimed to analyze and evaluate the infection of T. evansi in semi-captive Sumatran elephants in Way Kambas National Park, Indonesia, using various diagnostic tests. The prevalence of T. evansi in a total of 53 Sumatran elephants was estimated using a card agglutination test for trypanosomiasis (CATT) in 2016. A longitudinal study was later conducted in 2019 using Giemsa stained blood smear (GSBS) and polymerase chain reaction (PCR). The results showed that 26.4% of samples (14/53) were positively detected by both CATT in 2016 and PCR in 2019, while GSBS was unable to detect the parasites in all samples. Furthermore, four individuals were confirmed to have persistent infections. This study concluded that the ability of CATT and PCR were more convincing over GSBS for the diagnosis of sub-clinical trypanosomiasis in Sumatran elephants. However, it is recommended to use a combination of CATT as a screening tool and PCR as a confirmatory test for reliable results.
IN VITRO DEVELOPMENT OF Ascaridia galli EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Mubarokah, Wida Wahidah; Kurniasih, Kurniasih; Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12978

Abstract

The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
THE EFFECT OF APUS BAMBOO (Gigantochloa apus) LEAVES INFUSION TO MORTALITY RATE AND MORPHOMETRY OF Haemonchus contortus ADULT WORM IN VITRO Widiarso, Budi Purwo; Nurcahyo, Wisnu; Kurniasih, Kurniasih; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8167

Abstract

This present project was carried out to determine the effect of apus bamboo (Gigantochloaapus )leaves infusion to mortality rate and morphometry of Haemonchus contortus adult worms in vitro. The formulated tools were collected through camera, 3 ml syringe, object glass, deck glass, mortar, drip dropper, waterbath, microscope, stopwatch, counter check, oven, erlenmeyer flask, electric scale, surgical scissor, and pipette. The allocated materials used in this studywere bamboo leaves infusion, Haemonchus contortus adult worms, aquadestilata, ethanol and NaCl 0.62%. The research was firstly concernedwith making 0.1% and 1% apus bamboo leaves infusion. The samples were obtained from Haemonchus contortus collection. Adult worms were obtained directly from abomasum of naturally infected goats that were cut at Animal Slaughter House (RPH). To investigate the matter, mortality rates of adult worms at various doses and times of observation as well as differences in Haemonchus contortus morphometry were analyzed using ANOVA. The findings disclosed that apus bamboo leaves infusion demonstrated a significant difference in the mortality rate of adult worms Haemonchus contortusat various doses and times of observation. This report presented the findings of research that the best dose to increase the mortality of worms was 1% bamboo leaves infusion. Moreover, apus bamboo leaves infusion affected the morphometry of Haemonchus contortus adult worms, especially in body length, cervical papilla width, and spicules length in males, and body length, cervical papilla width, and vulvar length in females.
Study of Toxoplasmosis Infection in Human and Related to Animal in Banda Aceh Hanafiah, Muhammad; Kamaruddin, Mufti; Nurcahyo, Wisnu; Winaruddin, Winaruddin
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9813

Abstract

The research has been done to know prevalence of toxoplasmosis in livestock, to search the source of infection that have potency to cause toxoplasmosis in human and to make a map of toxoplasmosis infection area in Banda Aceh. The method used in this research was serologic test, Card Aglutination Test (CATT). Data of fertile women in this study are collected through questioner. The result of this research showed that the toxoplasmosis prevalency number of society in Banda Aceh was 3.15%, while at livestock were varied respectively: goats 40%, chickens 25%, cattles 23%, duck 20%, cats 16%, buffalos 15%, and sheeps 10%. The potencial sources of to coures toxoplasmosis in human are: goat, chicken, cattle, duck, cat, buffalo, and sheep. The area found toxoplasmosis in humans and animal were: Baiturrahman, Kuta Raja, Ulee Kareng, Kuta Alam, and Syiah Kuala. Model toxoplasmosis infection patterns in human related to livestock and animal in Banda Acheh are : Y ( POSTOKSO) - 1,55688 + 2,65280 AYA + 1,17709 JDG + 4,28482 KUC - 3,74609 MEM
Study of Tissue Cyst Formation Time of Toxoplasma gondii in Mice Hanafiah, M.; Nurcahyo, Wisnu; S, Sumartono
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3132

Abstract

The purpose of the research was to study a tissue cyst formation time Toxoplasma gondiiexperimentally. A number of 84 mice were divided randomly into four groups. Each group consisted of 21mice. The mice of the group I were infected with 101, II with 102 and III with 10 tachyzoites respectivelyintraperitoneally, whereas the group IV as a control (not infected with tachyzoites). All infected micewere treated with sulfadiazine, 15 mg/mouse per oral diluted in drinking water, for 5 days. On first untiltwenty first day after treatment one mouse of each group was necropsied. Liver, lymph, kidney, lung,heart, brain, or diaphragm muscle were then taken for histological preparations. Data on tissue cystformation time was analysed descriptively. The research revealed that innoculation with tachyzoites 103cyst could be found on day 14th after infection of liver, 102 cyst was found on the 6 day of liver, in day7th in heart and brain on day 10th of after infection, 103 cyst was found on day 4th inheart and brain in day 7thth in liver, day 6 after infection, while in the control dosage there is no formation similar to cyst found.Keywords: cyst, tissue, T. gondii, mice th1
FAKTOR RISIKO INFEKSI Toxoplasma gondii PADA KUCING DOMESTIK YANG DIPELIHARA DI YOGYAKARTA Hanafiah, Muhammad; Nurcahyo, Wisnu; Prastowo, Joko; Hartati, Sri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2792

Abstract

Tujuan penelitian adalah menentukan kemungkinan faktor-faktor risiko terhadap kemunculan toksoplasmosis. Sebanyak 132 sampel serum darah diambil pada kucing lokal di Yogyakarta. Data-data epidemiologis seperti asal usul kucing, ras kucing, jenis kelamin, umur, lokasi sistem pemeliharaan, jenis pakan, dan frekuensi diare diberi kode untuk mempermudah analisis, kemudian dimasukkan, disimpan, dan dianalisis dengan program Statistix Versi 7 (Analytical Sofware inc). Analisis data dilakukan secara bivariat (Chi-square (2), dan kekuatan asosiasi (OR), dan multivariat (regresi logistik). Hasil penelitian menunjukkan bahwa faktor-faktor yang berpengaruh terhadap adanya toksoplasmosis pada kucing berdasarkan analisis bivariat adalah pembersihan kotak pasir 1 kali sehari dan mandi 2-3 kali seminggu sedangkan faktor-faktor yang memiliki peluang meningkatkan seropositif toksoplasmosis berdasarkan analisis multivariat adalah pemeliharaan kucing yang bebas di dalam rumah, dimandikan lebih dari 1 kali seminggu, dan dimandikan lebih besar dari 1 bulan sekali.
IDENTIFIKASI TOKSOPLASMOSIS PADA FESES KUCING SECARA MIKROSKOPIS DAN SEROLOGIS Nurcahyo, Wisnu; Prastowo, Joko; P, Priyowidodo
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2639

Abstract

Penelitian ini bertujuan mengetahui tingkat prevalensi Toxoplasma pada kucing dan menyusun basis epidemiologis pada kucing yang sangat penting dalam peranannya sebagai hospes definitif toksoplasmosis. Sampel serum darah dan feses kucing digunakan dalam penelitian ini. Metode pemeriksaan menggunakan card agglutination Toxoplasma test (CATT) Pastorex Toxo kit dan metode sentrifus. Data yang dip eroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa tingkat prevalensi Toxoplasma dengan pemeriksaan serum darah kucing sebesar 6,8% dan pemeriksaan feses sebesar 9,4%. Infeksi toksoplasmosis yang terjadi pada kucing secara umum dari pemeriksaan klinis tidak mempunyai gejala yang spesifik.
Co-Authors Devita Anggraeni Estoepangestie, Agnes Theresia Soelih Imamudin, Kurniasih Imron, M.A. Joko Prastowo Kurniasih Kurniasih Lucia Tri Suwanti, Lucia Tri M. Hanafiah Mubarokah, Wida Wahidah Mufti Kamaruddin Muhammad Hanafiah Nursalim, Muhammad Tauhid Priyowidodo P, Priyowidodo Purnamasari, Kartika Shatrie, Dikry N Sri Hartati Sumartono s Widiarso, Budi Purwo Widiarso, Widiarso, B. P. Winaruddin Winaruddin