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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 32 Documents
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Search results for , issue "Vol 10, No 3 (2010)" : 32 Documents clear
PERKEMBANGAN OOSIT IKAN PATIN SIAM, Pangasianodon hypophthalmus Sauvage, 1878 (PANGASIIDAE; SILURIFORMES) Evi Tahapari; Bambang Iswanto
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.748

Abstract

Pangasianodon hypophthalmus is an introduced species of fish culture and fisheries resources in Indonesia. Reproductive biology aspects, e.g. maturity, plays an important role on the fisheries management. There was no detail information of oocytes development of P. hypophthalmus. The aim of this study is to find out the detail of its oocytes development through the observation of whole oocytes and ovarian histological slides microscopically. The results showed that the rhythm of oocyte development of P. hypophthalmus was grouped synchronism, assigned by the presence of two oocyte groups in the mature females, i.e. the large and maturing oocytes which would be spawned soon, and the small ones as the stock and still unyolked for the next spawning. The oocyte development could be devided into five stages, i.e. stage 1 (chromatin nucleolar and perinucleolar) with oocytes diameter less than 300 urn, stage 2 (yolk vesicles and cortical alveolar) with oocytes diameter of 230-660 urn, stage 3 (yolk granules) with oocytes diameter of 430-1,100 urn, stage 4 (migratory nucleus and hydrated) with oocytes diameter of 950-1,260 urn, and stage S (atretic) with oocytes diameter less than 800 m
PERTUMBUHAN AKAR DUAPULUH GENOTIP PADI GOGO PADA KAHATFOSFOR DAN CEKAMAN ALUMINIUM Tintin Suhartini
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.753

Abstract

Phosphorous insufficiency is a limiting factor for rice production. P deficiency in acid soil of tropical regions causing fixation of Al. Because of it, the giving P fertilizers in acid soil become not efficient. To exploit tolerance varieties to P deficiency and Al toxicity are one of the solutions to decrease P fertilizers. The objective of this experiment was to screen twenty upland rice genotypes for P deficiency and Al toxicity, and double stress Al toxicity and P deficiency in culture solution. The experiment was arranged in a split plot design with three replications. The main plots were aluminium treatment (without and 45 ppm Al), the sub plots were P treatments (0 ppm P, 0,5 ppm P, 5 and 10 ppm P) and twenty genotypes of upland rice were sub-sub plot. The root length and root dry weight characters were used to identify genotypes tolerant to P deficiency and Al toxicity. The results indicated that based on the root length character, Sentani and K36-5-1-1-1 were tolerant to Al toxicity and double stress P deficiency and Al toxicity; while based on the root dry weight character, K36-5-1 -1-1 and NIL-C443 were tolerant to P deficiency, Al toxicity and double stress P deficiency.
PENGARUH INTERAKSI HARA NITROGEN DAN FOSFOR TERHADAP PERTUMBUHAN TANAMAN JAGUNG (Zea Mays L) PADA TANAH REGOSOL DAN LATOSOL Arifin Fahmi; Sri Nuryani H Utami; Bostang Radjagukguk
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.744

Abstract

Nitrogen (N) and Phosphorus (P) are essential nutrients for plant.The deficiency one of these nutrients will be limiting the plant growth.A glass house experiment with one treatment factor was conducted to study the effect and interaction of N and P fertilizers application on maize growth in regosol and latosol soils. The results show that the interaction of N and P fertilizers application(N 1 P 1) on regosol had a significant influence to plant height on 3 to 7 weeks after planting, whilst on latosol had a significant influence to plant height on 1 to 5 weeks after planting. The shoot dry weight, root dry weight and total dry weight of maize on regosols and latosol soils were significantly influenced by the interaction of N and P fertilizers application (N 1 P 1). There were a positive correlation between N and P fertilizers application on both soils types to the total plant dry weight.
OPTIMASI FREKUENSI PEMBERIAN VITAMIN C PAD A PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPES VIRUS (KHV) PADA IKAN MAS (Cyprinus carpio Linn.) Taukhid Taukhid; Angela Mariana Lusiastuti; Kusumasari Suryadi; Rosidah Rosidah; Gunawan Setiadharma
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.749

Abstract

The research with objective to understand optimization frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) feeding to control the Koi Herpes Virus (KHV) disease infecting common carp has been done in Fish Disease Laboratory Fishes were reared in plastic container (80 litres), with density of 20 fish sized 10 gram in average. The treatments were: (A) daily application, (B) three daily application, (C) five daily application, and (D) without vitamin C as a control. Examined fishes were challenged to KHV infection after the 21 days rearing period by cohabitation method for 2 weeks. Observations been done on behaviour, clinical signs and mortality of fishes. The results showed that the highest survival rate was found on the application o vitamin C given every 3 days (50.0%); followed by every day (12.5%), every 5 days (7.5%), and the lowest was found on contro group (1.3%). Control techniques in the case of KHV carp populations through the provision of vitamin C immunostimulatory conducted regularly since well before the existence of KHV infection provides the best protective level.
KOMPOSISI JENIS DAN JUMLAH BURUNG LIAR YANG DIPERDAGANGKAN DIJAWA BARAT Tri Haryoko
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.754

Abstract

Wildbird trade is a threat to the conservation of birds population in Indonesia. The main causes of wildbirds extinction are habitat loss or habitat destruction and poaching for trade.The aim of this study was to identify trade of wildbird species in West Java. The survey was carried out in October to November 2008.A total of 2080 birds (consisted of 67 species and 29 family) were traded in Bandung, Garut and Tasikmalaya areas.Bird markets in those three areas traded 1051, 496 and 533 birds respectively. The result of one way analysis of variance (anova) using SPSS 13.0 F , ll15)= 2,398 < Ftabel = 3,042, showed no significant different in the above mentioned three bird markets.
ELISITASI PENINGKATAN PRODUKSI AJMALISIN OLEH KALUS Catharantus roseus (L.) G. Don. Marina Silalahi
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.745

Abstract

An experiment to study the effect of soft fraction derived from Saccharomyces cerevisiae (Hansen) to elicit ajmalicine production by Catharanthus roseus (L.) G. Don. callus cultures has been conducted. Callus were induced from leaf segment and grew on Zenk medium supplemented with 2,5 x l0M NAA dan 10 M BAP respectively. Callus on the third subculture level were elicited with soft fraction derived from S. cerevisiae. The following concentrations of elicitor tested were 0, 0,5, 1,0 and 2,5 % (g/v), and the harvesting times were 0, 18, 36 and 72 hour respectively. Influence of elicitor to production of ajmalicine by the callus were analyzed qualitatively and quantitatively by using high performance liquid chromatography (HPLC). A significant increasing of ajmalicine content in the callus (303,475 ± 5,602 ug/gDW) was achieved by addition of elicitor of 0,5 % (g/v) after 36 hour. This study showed a significant increase of ajmalicine production in C. roseus callus cultures after being challenged with elicitor derived from S. cerevisiae i.e. 69,334%.
ENDOFIT Taxus sumatrana (Miquel) de Laubenfels DAN POTENSINYA DALAM MEMPRODUKSI SENYAWA BIOAKTIF SEBAGAI SUMBER ANTIOKSIDAN Harmastini Sukiman
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.750

Abstract

Taxus sumatrana (Miquel) de Laubenfels is known as an endanger forest tree species grown at Kerinci National Park in Jambi,Indonesia. This plant was known potential on producing taxol. Taxol is a bioactive compound, could be used as antibacterial agent and recently confirmed to cure cancer cells. For the first time in 1960, Arthur Barclay found the taxol compound from Taxus sp.(pacific yew). However to isolate the bioactive compound, huge amount of tree biomass is needed. Research on endophytes microbes which are isolated from inner tissue of Taxus sp. declared that those microbes have potential on producing antioxidant agent for drug discovery. Isolation and conservation of endophytes and selecting its potential is promising the novel of finding new drug that may be effective for treating the newly developing diseases in human. Fifteen isolates of Taxus endophytes have been successfully studied on their ability on producing antioxidant. The results showed that endophytes fungus isolates TsC-17, isolated from Taxus sumatrana grown in Cibodas Botanical Gardens-LIPI, could produce extracellular bioactive compound which performed activity of suppressing free radical, is significantly better compared to intracellular bioactive compound eventhough it is not as high as in vitamin C. The activity of suppressing free radical resulted from bioactive compound of endophyte fungus isolates TsC-17 was 20% for intracellular bioactive compound, while it was 60% for extracellular bioactive compound and 90% for vitamin C.
KERAGAMAN GENETIK BEBERAPA KLON DURIAN (Durio zibethinus Murray) ASAL JAWA BARAT BERDASARKAN SIDIK RANDOM AMPLIFIED POLIMORPHIC DNA Kusumadewi Sri Yulita; Muna Murnianjari
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.741

Abstract

Durian (Durio zibethinus) is one of the most popular tropical fruits in SE Asia. Indonesia has several local clones that have not yet been widely introduced to local fruit markets. This present study aimed to assess genetic diversity of 17 clones of durian from West Java based on RAPD fingerprints. Thirty RAPD's primers were initially screened and four were selected for the analysis.These four primers (OPA 13, OPD 8, OPN 6 and OPA 18) generated 63 scorable bands to which 100% of them were polymorphic.OPA-13 at 700bp was exclusively possessed by Tambleg clone and other bands were shared among the other clones. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic similarity value among genotypes was 0.15-0.73 suggesting high genetic variation among them. Results from genetic diversity analysis based on plant propagation system showed a higher genetic diversity value in occulating (87.30%) plants than that of grafting (60.32%).
EFEKTIVITAS MULTIVITAMIN DAN MENIRAN (Phyllanthus neruri L.) DALAM MENURUNKAN STRES PADA DOMBA SELAMA TRANSPORTASI Aryani S Satyaningtijas; Andriyanto Andriyanto; Armando Ramadhoni; Yulia Suci; Fitriana Dewi; Abadi Sutisna
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.755

Abstract

Sheep is one of achievable animal protein sources. Therefore, they need to be transported to/in different places to fulfill the demand of mutton. This research was conducted to know the impact of transportation on stress as presented by leucocyte differentiation (N:L ratio). Blood were taken from sheep without stress transportation as a control for 12 hours.In this research, 12 sheep were divided into 3 groups: KP (control with stress),PA (stress with combination multivitamin and meniran - Phyllanthus niruri L.), PB (stress with combination multivitamin).Blood samples were taken at 0 hour (pre-transportation), 4h, 8h, 12h (during transportation) and 24h, 48h, 72h (post-transportation) to observe leucocyte differentiation. The results showed that ratio N:L (as stress indicator) were higher at all time periods during transportation and after the transportation. Sheep as a control group had reached the peak of ratio N:L at 0 h, meanwhile sheep with combination of multivitamin and meniran at 12 h and sheep with multivitamin only at 8. It means that the sheep with combination of multivitamin and meniran treatment was more effective to response the transportation stress.
PENINGKATAN KADAR LOVASTATIN ANGKAK OLEH Monascuspurpureus KO-KULTUR DENGAN Endomycopsis burtontt Danik D Asadayanti; B Sri Laksmi Jenie; Harsi D Kusu aningrum; Novik Nurhidayaf
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.746

Abstract

Lovastatin is a bioactive material of statin groups and has been used to reduce cholesterol through inhibiting HMG Co-A reductase enzyme activities. Three indigenous strains of Monascus purpureus and three mutans were used in this study produced lovastatin at the range of 0,1 - 1,42%. The objectives of this study were to increase lovastatin productions by co-cultured with several concentrations of Endomycopsis burtonii. M. purpureus (10 cfu/ml) was co-cultured with various concentrations of E. burtonii (10M0 ) cfu/ral at three different feeding times (day 2, 4, and 6). Feeding times and concentrations of E. burtonii significantly increased production of lovastatin by four strains of M. purpureus (JMBA5K, AID, JMBA and TOS). The highest production of lovastatin was achieved by M. purpureus TOS co-cultured by E. burtonii at 10 cfu/ml added at day 6. Expression of the genes that responsible for lovastatin production were analyzed by PCR and RT-PCR method. The phenotypic character of M. purpureus TOS with high lovastatin production was conformed by the high intensity of its gene expression.

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