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BERITA BIOLOGI
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Articles 30 Documents
Search results for , issue "Vol 10, No 6 (2011)" : 30 Documents clear
REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Yohana C Sulistyaningsih; Alex Hartana; Utut Widyastuti; Hamim Hamim; Suharsono Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1940

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.
IDENTIFIKASI GEN PENYANDI PIREN DIOKSIGENASE PADA ISOLAT BAKTERI PENDEGRADASI PIREN FA Febria; Jamsari Jamsari; N Nasir; N Nurhidayat
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1946

Abstract

The pyrene dioxygenase coded by gene is an indicator of bacterial isolates capabilities in pyrene degradation. The encoded gene of pyrene dioxygenase can be amplified and detected in Pseudomonas sp. PyrA2 and Burkholdoriasp. PyrA4 isolates, using primary specific Diox which designed based on PhdF gene sequence, the coding gene of pyrene dioxygenase in Mycobacterium vanbalenii PYR-1. The sequence alignment of pyrene dioxygenase putative gene in both of bacterial isolates with the sequence of pyrene dioxygenase coding gene in M. vanbalenii PYR-1 shows the similarity percentage of 41 % and 42% with Pseudomonas sp. PyrA2 and Burkholdoria sp.PyRA4.
KEEFEKTIFAN BAHAN PELINDUNG ALAMI DALAM MEMPERTAHANKAN INFEKTIVITAS Spodoptera exigua NUCLEOPOLYHEDROVIRUS (SeNPV) Samsudin Samsudin; Teguh Santoso; Aunu Rauf; Yayi Munara Kusumah
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1932

Abstract

Spodoptera exigua nucleopolyhedrovirus (SeNPV) is a viral pathogen of onion caterpillar S. exigua with high pathogenicity. One of the major constraints to the use of SeNPV for biocontrol of onion caterpillar is its sensitivity to ultraviolet (UV) degradation. The purposes of this research were to determine the effect of sunlight exposure on the virulence of SeNPV and to find out the effective natural UV protectant to maintain the SeNPV virulence. The results showed that the sunlight radiation affects the SeNPV infectivity. Addition of 1% of coconut shell charcoal, lampblack, husk charcoal, yam flour, molasses, yam filtrate, turmeric filtrate and green tea filtrate to the SeNPV suspension were found to be effective as UV protectant. Coconut shell charcoal, lampblack and husk charcoal are activated carbon that can absorb UV light. Yam filtrate is a natural ingredient that contains saponins and is able to protect SeNPV particles as reflectance. While molasses, turmeric filtrate and green tea filtrate containing flavanoid serve as a protective virus particles and UV absorber.
HETEROSIS, HETEROBELTIOSIS DAN TINDAK GEN KARAKTER AGRONOMIK KEDELAI {Glycine max (L.) Merrill} Ayda Krisnawati; MM Adie
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1952

Abstract

Most of the soybean {Gfycine max (L.) Merrill (varieties in Indonesia are produced through hybridization. Expected value of heterobeltiosis,heterosis and the gene action is needed to know to optimize the development strategy of soybean varieties. The experiment was conducted in The Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian (Balitkabi) greenhouse. The first stage was to form Fl hybrid seed,which was done from May to July 2009; the second stage was for the assessment of Fl plants (August-November 2009). Heterosis,heterobeltiosis and the potential ratio are calculated from the 18 crosses combinations derived from 6 parental genotypes. Data used include the flowering day (days), maturity day (days), plant height (cm), number of branches/plant, number of node/plant, number of pods/plant,seed yield/plant (g) and SO seeds weight (g). Cross combinations that have the highest heterosis values are on the flowering day, maturity day, number of branches/plant and number of node/plant; heterobeltiosis also showed the highest value on the same agronomic characters.Heterosis value of the character of seeds per plant ranged from -36.50% to 75.49%, while for heterobeltiosis ranged from -48.68% to 58.31%. The highest heterosis value was indicated by the Fl from crosses Willis x Malabar. The highest heterobeltiosis value was indicated by crosses of Willis x Grobogan, which means that these crosses have the 58.31% higher yield than the best parents. The soybean yield is controlled by dominant genes and over-dominant. Combination crosses Willis X MLG 0706 (reciprocal) has a higher potential ratio for die character of the number of pods and seeds yield/plant compared to the value of the other potence ratio. Development of soybeans with the use of hybrid heterosis phenomenon still needs to be assessed in terms of economic feasibility.
SIMTOMATOLOGI DAN WAKTU KEMATIAN RAYAP Macrotermes gilvus Hagen (ISOPTERA: FAMILI TERMITIDAE) SETELAHINFEKSI CENDAWAN Metarhizium brunneum Petch Muhammad Sayuthi; Teguh Santoso; Idham Sakti Harahap; Utomo Kastosuwondo
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1941

Abstract

The potential of entomopathogenic fungus Metarhizium brunneum Petch as biocontrol agent to termite Macrotermes gilvus Hagen has been tested in the laboratory.The purpose of this research is to study the symptomatology and lethal time of the termite M. gilvus fungus after infection by M. brunneum. The density of conidia at 1.21xlO6/mL showed as effective concentration in causing M. gilvus mortality, when compared to 1.08><106conidia/mL, and control. The infection stage of M. brunneum on the host until the death of its host occurred on day 2,and the sporulation of M. brunneum on the surface of the host integument occur on day 4. The entire surface of M. gilvus was covered by the mycelium and conidia of M. brunneum on day 7. Lethal time 50% of termite population of M. gilvus (LT50) was achieved in 5 days(5.14), and LT95 achieved on day 10 (10.03).
KAJIAN OZONISASI (O3) TERHADAP KARAKTERISTIK KUBIS BUNGA {Brassica oleracea var. botrytis) SEGAR SELAMA PENYIMPANAN PADA SUHU DINGIN Ali Asgar; A T Sugiarto; Sumartini Sumartini; D Ariani
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1947

Abstract

The aims and objectives of this study are to determine the effect of ozonization on the characteristics of cauliflower (Brassica oleraceae var.botrytis) during cold storage.The benefit of this research is to prolong the storage period of cabbage flowers with characteristic and quality be appropriate to consumers. The design used is the Randomized Block Design with 6 replications. The treatments consisted of 0 ppm ozone concentration bear leaves, 1.5 ppm ozone concentration without leaves, 1.5 ppm ozone concentration bearing leaves, and 1.5 ppm ozone concentrations without leaves.The parameters observed were weight loss, hardness, moisture content, vitamin C, heavy metals Hg and Ag,number of microorganisms, organoleptic color, freshness and appearance. The results showed that the best treatment was 1.5 ppm ozoneconcentrations with cabbage flowers and leaves. This was caused by ozone acts in sterilization (ozone can kill a variety of pathogenic microorganisms such as bacteria, viruses and fungi), deodorant (ozone eliminating odors caused by a variety of organic compounds and microorganisms), decoloration (ozone can eliminate organic color), and degradation (ozone can break down various organic compounds and heavy metals).
PENGARUH PEMUPUKAN BERIMBANG TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KENTANG (Solatium tuberosum L.) VARIETAS GRANOLA Syafri Edi; Endrizal Endrizal
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1935

Abstract

Potato is one of the main vegetables in Kerinci District. Development of potato plants is supported by the availability of land, agro ecosystem, labor and market opportunities as well as conductive local government policies. Potato farmers do not fully implementing the recommended farming technologies, such as the use of good seed, balanced fertilizer, crop rotation, pest control, harvesting and post harvest, so the production and reception of farmers is still low. The research took place in Mount Seven Village, Pelompek Kerinci District, Jambi Province which is background by agro-ecosystem dryland upland wet climate (LKDTIB), with soil type andisol at an altitude of 1400 m above sea level. The study aimed to see the effect of balanced fertilization on the growth and yield of potato. Potato used is Granola variety fourth generation derived from the Parent Seed Potato Institute in Kayu Aro. Assessments carried out from February until July 2008, with the treatment as follows: Introduction Package (A): SP-36 450 kg, 200 kg Urea, ZA 200 kg, 300 kg of KC1, NPK 100 kg and 10,000 kg of organic fertilizer/ha. Farmers Package (B): SP-36 750 kg, 400 kg Urea, 250 kg of KC1, and 400 kg NPK /ha. Fanners Package (C): SP-36 600 kg, 300 kg ZA, KC1 300 and 400 kg NPK /ha. The results showed that Package (A) gives the results of 24,320 kg /ha, higher than package (B) with the results of 18,240 kg/ha, while the Fanners Package (C) get the 19,520 kg/ha. High yield Package (A) is supported by the controlled growth of plants, the intensity and the population of pests and diseases and relatively more yield components than Package(B) and Package (C).
ANALISIS TINGKAT PENCEMARAN AIR DENGAN METODE INDEKS PENCEMARAN DI TELUK YOUTEFA, JAYAPURA, PROVINSI PAPUA Janviter Manalu; I Wayan Nurjaya; Surjono HS; Kholil Kholil
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1942

Abstract

Youtefa Bay is one of the potential natural recources, is located on the western side of Jayapura City,Papua province.The waters has beenutilized for fishery, transportation and ecotourisme.Youtefa Bay surroundings are settlements where the most of residents are fishermen and farmers.The aim of this study is to determine the pollution level of the Youtefa Bay,approached by pollution index method.The results showed that the water condition of Youtefa Bay was slightly to moderately polluted.Due to pollution, it is therefore very importance to periodically monitor the water quality of the Youtefa Bay to maintain its sustainability.
POLA KECENDERUNGAN PENANGKAPAN BURUNG-BURUNG LIAR BERNILAI EKONOMIS DAN IMPLIKASI KONSERVASINYA: STUDI KASUS DITANAH GROGOT, KABUPATEN PASER, PROVINSI KALIMANTAN TIMUR Rachmat Budiwijaya Suba; Aditya Rakhman; Rustam Rustam
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1949

Abstract

The study aims at quantifying trade scale of some economically wild birds and describing captured trends which have been practised by wild bird catchers. The study was conducted in Tanah Grogot, Paser District, East Kalimantan Province, from September to December 2008, by surveying wild bird market and interviewing catchers and sellers. We measured two main attributes of market dynamics; first, each species availability in the market as the percentage of market days sampled where the species appeared; second, daily abundance of a species as the monthly average number of each bird per day for all days sampled. Seven wild bird species that have been traded commodities for years were White-rumped Shama Copsychus malabaricus (25.41%; 602.25+70.79 individuals per month), Yellow-vented Bulbul Pycnonotus goiavier (11.47%; 111.25+12.50 individuals per month), Crested Myna Acridotheres cristatellus (12.30%; 73.25+10.44 individuals per month), Spotted Dove Streptopelia chinensis (5.73%; 22.00+12.83 individuals per month), Hill Myna Gracula religiosa (12.30%;12.00+0.82 individuals per month), Greater Green Leafbird Chloropsis sonneratii (7.38%; 11.75+2.50 individuals per month) and Magpie Robin Copsychus saularis (6.56%; 8.75+1.71 individualsper month). Specific capture location within Paser District could reflect distribution and habitat preference of each bird species.
KORELASIANTAR-KARAKTER DAN SIDIK LINTAS ANTARA KARAKTER AGRONOMIDENGAN HASIL KEDELAI {Glycine max (L.) Merrill} Lukman Hakim
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1936

Abstract

A total of 27 soybean {Glycine max (L.) Merrill} genotypes including check variety (Kawi) were evaluated on dry land at Ngale Experimental Farm, Ngawi, East Java during early rainy season of 2009/2010. The experiment was conducted in a randomized complete block design with three replications. Plot size was 4 m x 5 m, plant spacing was 40cmx20cm, each hill contained two plants. A correlation and path coefficient analyses were used in order to determine the association among characters and quantify the direct and indirect effects of agronomic traits on seed yield. The study has identified 3 varieties, i.e. Kaba, Wilis and Tanggamus produced the highest yield (>1.6 t/ha) and had good adaptation on dry land. Two varieties (Grobogan and Argomulyo) having large seed size (>15 g/100 seeds), and four varieties,i.e. Tidar, Grobogan, Gepak Ijo and SHR-W60 had early maturity (81days). Therefore, these varieties can be used as source of parents on breeding program. Among the agronomic characters, plant height, number of pods per plant and harvest index were positively and significantly correlated with grain yield. The direct effect of plant height, number of pods per plant and harvest index to grain yield as indicated by path coefficient were the highest, while other causal effects to grain yield were small or negative. Based on both analyses, soybean genotypes with high grain yield should have sufficient plant height, high number of pods per plant and high harvest index. Therefore, plant height, number of pods per plant and harvest index can be suggested for selection criteria in selecting high yield soybean genotypes on dry land.

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