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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 26 Documents
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Search results for , issue "Vol 12, No 3 (2013)" : 26 Documents clear
KARAKTERISTIK GAMBUT RAWA PENING DAN POTENSINYA SEBAGAI BAHAN PEMBAWA MIKROBA Prihastuti, Prihastuti
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.640

Abstract

This is a descriptive analytical research to describe characteristic of peat soils of Rawa Pening (a natural lake in Central Java, Indonesia). The purpose of this study was to determine the potency of peat soils as a microbial carrier. The parameters observed were chemical properties (pH, levels of C, N, P, K and CEC) and biological properties (total of soil microbial population), as well as its amendments by sun drying,ovens drying and sterilization process by autoclaving (121oC, and 1.5 atm for 15 minutes). The results showed that the Rawa Pening peat soil content consisted of high organic C, medium-high N, low-medium P, high K and high CEC. Total of soil microbial population was quite high, but it was reduced by sun drying and autoclave sterilization. The population of soil microbial was pressured by oven drying, but the process makes it a low pH caused by cell lysis. The Rawa Pening peat soils had physical properties that were not easily changed, is also not prone to changes in pH during the process by sun drying and sterilization by autoclave. It was concluded that the Rawa Pening peat soils have a potential to be a microbial carrier, since it contained high organic matter, and relatively easy to process.
TINGKAT HUBUNGAN ANTARA VARIABEL PENDUGA BOBOT DAGING (CARCASS CUTABILITY) KARKAS SEGAR SAPI SIMPO DAN LIMPO JANTAN Sakti, Awistaros Angger; Panjono, Panjono; Rusman, Rusman
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.621

Abstract

Estimation of carcass cutability is an important factor in beef marketing. This research was conducted to observe the relationship among variables of carcass cutability estimation in hot carcass of SimPO (Simmental-Peranakan Ongole) and LimPO (Limousin-Peranakan Ongole) cattle. Eighty three bulls were used, consisted of 51 SimPO and 32 LimPO cattle. The data collected were age, body weight, carcass weight (X1), fat thickness (X2), ribeye area (X3), percentage of visera fat (X4), and carcass cutability. The data were analyzed using Independent Sample T-test, correlation 2-tailed, enter and stepwise regression analysis, and estimation curve. There were no different of age, body weight, X1, X2, X3, X4, and carcass cutability among SimPO and LimPO. There was a positive correlation between X1, X2, X3, X4 and carcass cutability (P<0,05), except to LimPO which had negative correlation for X4. The regression equation and determination coefficient of SimPO and LimPO were Y=-24,726+0,916X1; R2=0,948 and Y=-28,461+0,929X1; R2=0,996, respectively. The result indicated that the best equation for SimPO was a linear equation (R2=0,948), and a quadratik equation (R2=0,996) for LimPO.
PURIFIKASI DAN KARAKTERISASI ENZIM PEKTINASE DARI ASPERGILLUS USTUS BL5 Yopi, Yopi; Rahmani, Nanik; Andriani, Ade; Dewi, Fitria; Meryandini, Anja
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.646

Abstract

Pectinase is an enzyme that could hydrolyze pectin into galacturonic acid. Natural pectinase was produced by microbes such as bacteria, yeast, fungi and Actinomycetes. Application of pectinase in industry were mainly in juice industry, textile, pulp, tea, cocoa and coffee fermentation. In this research, we conducted purification and characterization of pectinase produced by Aspergillus ustus BL5 in submerged fermentation using commercial pectin. The result showed that the optimum of pectinase production was reached at 120 hours fermentation process with specific activity 0.59 U/mg. The crude extract of pectinase was then concentrated using PEG 6000 and purified by Sephadex G-75 gel filtration chromatography. There were 2 fractions contained pectinase which the activity was 4.15 U/mg (pectinase A) and 3.3 U/mg (pectinase B), respectively. Compare to crude extract, the yield product of pectinase A and B increased 6.94 and 5.53 times, respectively. The purified pectinase A have optimum temperature at 50 oC and optimun pH at 5.
PURIFIKASI DAN KARAKTERISASI ENZIM PEKTINASE DARI Aspergillus ustus BL5 Yopi Yopi; Nanik Rahmani; Ade Andriani; Fitria Dewi; Anja Meryandini
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.646

Abstract

Pectinase is an enzyme that could hydrolyze pectin into galacturonic acid. Natural pectinase was produced by microbes such as bacteria, yeast, fungi and Actinomycetes. Application of pectinase in industry were mainly in juice industry, textile, pulp, tea, cocoa and coffee fermentation. In this research, we conducted purification and characterization of pectinase produced by Aspergillus ustus BL5 in submerged fermentation using commercial pectin. The result showed that the optimum of pectinase production was reached at 120 hours fermentation process with specific activity 0.59 U/mg. The crude extract of pectinase was then concentrated using PEG 6000 and purified by Sephadex G-75 gel filtration chromatography. There were 2 fractions contained pectinase which the activity was 4.15 U/mg (pectinase A) and 3.3 U/mg (pectinase B), respectively. Compare to crude extract, the yield product of pectinase A and B increased 6.94 and 5.53 times, respectively. The purified pectinase A have optimum temperature at 50 oC and optimun pH at 5.
PERKEMBANGAN EMBRIO DAN LARVA IKAN PATIN NASUTUS (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) Bambang Iswanto; Evi Tahapari
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.622

Abstract

Patin nasutus (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) is a potential pangasiid catfish species to be cultured in Indonesia.Many efforts has been done to explore the potency of P.nasutus for aquaculture purposes.However, basic knowledge is still very rare such as its embryonic and larval development.The present study aimed to understand the embryonic and larval development of P. nasutus resulted from induced spawning of P.nasutus brooders at Research Institute for Fish Breeding, Sukamandi. Those embryonic and larval development were observed microscopically.The results showed that diameter of the ovulated oocytes ranged 1.40-1.65 mm and fertilized eggs ranged 1.90-2.15 mm.The embryogenesis consisted of several stages as follow: morula, blastula, gastrula, organ developments and newly hatched larvae occured within periods of (minutes) 20-200, 190-220, 210-660, 600-1,140 and 1,800-2,160 after fertilization at water temperature of 28-29oC. Total length of the newly hatched larvae ranged 4.80-5.10 mm with anterior, posterior and dorsal parts of the yolk sac coloured black pigmentation. On caudal peduncle, the black spot existed since 36 hours after hatching was a strong character for P.nasutus larvae.Volume of the yolk sac was 2.65±0.14 mm3, for which 50% absorbed at 36 hours after hatching and relative fully absorbed at 78 hours after hatching. The larvae have complete morphology as adult after seven days. During early developmental stage, larvae showed cannibalism since the third day, corresponding to the first exogenous feeding.
ANALISIS KARBOKSIMETIL SELULOSA DARI BAKTERI Acetobacter xylinum DAN Acetobacter sp. RMG-2 Ruth Melliawati; Apridah Cameliawati Djohan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.642

Abstract

Bacterial cellulose has long been manufactured and used for industrial purposes and health. Bacterial cellulose more profitable than the cellulose plants because during the manufacturing process, they do not harm the environment. The purpose of this study was to identify differences of cellulose produced by Acetobacter xylinum and Acetobacter sp. RMG-2 from that produced by plants. The study was also aimed to determine superiority of carboxymethyl cellulose (CMC) produced by those bacteria. The medium HB was prepared for the production of cellulose from both bacteria. Bacterial cellulose preparation was carried out to obtain solid fine powder, followed by manufacturing carboxymethyl cellulose through several stages to obtain CMC powder. CMC analysis was performed for both bacteria and plants targeted on the surface structure of cellulose, the density of solids, viscosity CMC and functional groups. As a result, the surface fiber cellulose plants had a wider space than fiber cellulose bacterium. The density of solids of CMC A. xylinum, A. sp. RMG-2 and plant were 30.9998 g/cm3, 0.0079 g/cm3 and 0.9978 g/cm3 respectively. Viscosity of the CMC were of 5.78 cP, 5.25 cP and 5.91 cP for each A. xylinum,A. sp. RMG-2 and plant. CMC functional groups of bacteria has met the parameters of success as indicated by the infrared spectrum since it formed a methyl group, carboxyl group and the group of sugar. Cellulose Acetobacter sp. RMG-2 and A. xylinum cellulose can replace plants through the process of compound alkalization with sodium hydroxide, because the compound can lower the level of density of pores of cellulose fibers. The CMC resulting from bacterial cellulose as good as CMC plant and had characteristics resembling CMC plant.
PERGERAKAN LUTUNG JAWA Trachypithecus auratus (E. Geoffroy 1812) PADA FRAGMEN HABITAT TERISOLASI DI TAMAN WISATA ALAM GUNUNG PANCAR (TWAGP) BOGOR Eko Sulistyadi; Agus Priyono Kartono; Ibnu Maryanto
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.647

Abstract

Javan langur is protected spesies of primates that highly threatened. Habitat degradation as a major threat causes javan langur Trachypithecus auratus (E. Geoffroy 1812) populations living in isolated habitat fragments. The research was carried out to determine the daily movement of javan langur in TWAGP as form of adaptation in isolated habitat fragments. The study was conducted in May-June 2012 by using focal animal sampling method. Vegetation analysis was performed with a point centered quarter method. The results showed that there were three major land cover types used by the Javan langur, i.e. forest, transitional forest-cultivation and cultivation. The dominant activity occurred in transisional forest-cultivation habitat (48.19%). The highest proportion of activity wass resting (33.65%) followed by feeding (30.68%), moving (27.08%) and social activities (8.60%). Based on the variations of vegetation strata, the dominant activity performed at stratum C(4-20 meters) accounted for 80.07%.
KEANEKARAGAMAN TUMBUHAN PAKU TERESTRIAL DI HUTAN KOTA DKI JAKARTA Dwi Andayaningsih; Tatik Chikmawati; Sulistijorini Sulistijorini
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.638

Abstract

Urban forest is one of the green open area consisted of natural forest-like vegetation. It has suitable microclimate for understory habitat, including terrestrial ferns. The diversity of fern in three urban forest in DKI Jakarta is reported, i.e the urban forest at The University of Indonesia (UI) Campus, The Cibubur Arboretum and PT JIEP (Jakarta Industrial Estate Pulogadung). Vegetation analysis was conducted by purposive sampling method with a transect line of 200 m in each location with 15 plots of (3x3) m2 each. Fern determination was done based on herbarium specimens and literatures. Fern diversity was analyzed based on Shannon diversity index, and equality index. Canonical correspondence analysis (CCA) was performed to determine the correlation between fern diversity and abiotic factors. There were 18 fern species classified into 11 genera and eight families, at the UI urban forest and Cibubur Arboretum, but there was no fern in PT JIEP. Fern with the highest importance value index was Sphaerostephanos sp. (161.33%) in The UI urban forest, and Lygodium microphyllum (Cav.) R.Br. (71.63%) in Cibubur Arboretum. The CCA result showed that the fern diversity correlated to four abiotic factors i.e temperature,humidity, light intensity and soil pH.
KONDISI VEGETASI DI KAWASAN HUTAN KEBUN RAYA BALIKPAPAN Syamsul Hidayat
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.643

Abstract

Balikpapan Botanical Gardens (East Kalimantan) is an ex situ conservation area where some parts of its land is originated from the Sungai Wain Protection Forest. Most of the area had been burned in 1982 and 1998. For the development of botanical gardens, vegetation condition/status assessment in forest areas is needed. The study was conducted by sampling with nested plot. Ten observations transects, each consisting of five plots measuring 20 m x 20 m set at ten locations which were supposed to represent the community. Results of the study showed that the quality of the vegetation in general was low, with medium category for conservation index and less category for diversity index. The vegetation was dominated by pioneer species that were less in conservation value such as Macaranga spp., Melicope glabra (Blume) T.G.Hartley and Calicarpa sp. There were 56 individual trees of nine species of Dipterocarp family -which is the region's native plants- found within this area. As for seedling stage, it was also dominated by pioneer species and shrubs. Species enrichment through the development of botanical garden, are expected to increase the value of biodiversity and conservation index.
SKRINING DAN ISOLASI METABOLIT AKTIF ANTIBAKTERI KULTUR JAMUR ENDOFIT DARI TUMBUHAN Albertisia papuana Becc. Ahmad Fathoni; Muhammad Ilyas; Antonius Herry Cahyana; Andria Agusta
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.639

Abstract

Totally 15 isolates of endophytic fungi were obtained from leaves and young stems of a medicinal plant Albertisia papuana Becc.(Menispermaceae). The antibacterial screening of the ethyl acetate extract derived from the fungi cultures in potato dextrose broth (PDB)were performed on a non eluted Thin Layer Chromatography Bioautography assay (TLC bioautography assay). From the screening test, itwas found that the ethyl acetate extract of the fungus Xylaria sp. DAP-KRI-5 culture showed strong antibacterial activity against Staphylococcus aureus. The isolation of active metabolite based on the bioautography guided assay from ethyl acetate extract of the Xylaria sp. DAP-KRI-5, led us to identify phloroglucinol as a main antibacterial compound. The chemical structure of phloroglucinol was deduced from it spectral data, including UV-Vis, 1H and 13C-NMR, GC-MS and published data elsewhere.

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