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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 26 Documents
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Search results for , issue "Vol 13, No 2 (2014)" : 26 Documents clear
KARAKTERISASI PROTEASE BACILLUS SUBTILIS A1 INACC B398 YANG DIISOLASI DARI TERASI SAMARINDA Soeka, Yati Sudaryati; Sulistiani, Sulistiani
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.694

Abstract

Proteases is enzyme that breaks the peptide bond to produce amino acids and simpler peptides. This enzyme can be isolated from a variety of sources such as plants, animals and microbe. Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery, food, medicinal formulations and detergents. The objectives of the research was to determine the characteristics of the protease enzyme produced by strain A1, including incubation time, substrate concentration azokasein, the optimum temperature and pH also stability. The effect of some metal ions as activators or inhibitors of the protease enzyme activity measured with a spectrophotometer at ? 280 nm. Strain A1 was identified by using 16S rDNA sequencing and phylogenetic analysis based on Neighbor Joining method. Strain A1 protease activity was qualitatively demonstrated the presence of a clear zone around the colonies in the medium containing 1% skim milk. Result showed that the highest activity were incubation time of three days, temperature of 50 ºC and pH 8.5 were 87.35 U/mL, 83.44 U/mL and 93.11 U/mL, respectively. Effect of metal ions in the form of divalent and monovalent cations at a concentration of 1 mM on protease A1 activated by divalent cations CaCl2, MnCl2 while divalent cations CuCl2, HgCl2 and monovalent cations KCl, NaCl were inhibitors of each enzyme activity. Result from molecular identification based on 16S rDNA sequence and phylogenetic analysis using Neighbor Joining method suggested that strain A1 was Bacillus subtilis. The strain was registered in the InaCC collection (no. B 398).
HUBUNGAN PANJANG DAN BOBOT IKAN NILA LOKAL, BEST F5 DAN F6 DI PANGKEP, SULAWESI SELATAN PADA UMUR 60 HARI PEMELIHARAAN Kusmini, Irin Iriana; Gustiano, Rudhy; Putri, Fera Permata
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.685

Abstract

Best tilapia is breed fish developed by Research Center and Development of Freshwater Aquaculture (BPPBAT) Bogor that has been launched since 2008. Best tilapia is the third generation of gift tilapia. In order to obtain a better growth performance, BPPBAT continued the process of breeding, and to date we have obtained the sixth generations of Best tilapia. This study was aimed to determine growth of tilapia through observation of the relationship between the length and weight of local tilapia, F5 and F6 BEST which were maintained in brackish waters in the Pangkep area, South Sulawesi. Fish samples were taken at random, 30 fishes were taken from each population for length and weight measurement. Calculation of the length-weight of fish in the Pangkep area obtained regression coefficient (b) for local fish 2.4361; BEST F5 3.1077 and F6 3.0522. These values indicated that the local tilapia was negatively allometric while BEST was positively allometric, with local fish condition factor ranged between 0.471-1.334, F5; 0.468-1.479 and 0.498-1.534 F6 respectively. This results has indicating the normal condition of the fish. The length-weight relationship of fish had a value of determinant (R 2) ranged from 0.7829-0.9182, and indicated fish variations influenced by other variables was rather small and has suggested close relationship between length and weight.
VARIASI KARAKTERISTIK PERTUMBUHAN TACCA LEONTOPETALOIDES (L) KUNTZE (TACCACEAE) DI PULAU JAWA DAN PULAU-PULAU KECIL SEKITARNYA Syarif, Fauzia; Lestari, Peni; Wawo, Albert Husein
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.690

Abstract

An increasing of population, land conversion, and the behaviour changes in many crops due to global warming could produce a significant negative effect to food security in many countries, including Indonesia. In order to maintain the availability of food, we should find out alternative foods based on corp comodities that can adapt to future agricultural land conditions. One species to be promoted for that purpose is Tacca leontopetaloides (taka). This study was aimed to determine growth characteristics variations of taka in Java and its surrounding small islands through exploration and interview methods. Results indicated that there were two morphological variations of taka based on the canopy colour, namely green and purple. Taka found growing in coastal areas up to100 m above sea level. The light intensity and soil type greatly affected their growth. These plants lived either solitary or in a groups, grow well on sandy soil to clay, under the shade and open areas. Species around taka allegedly contributed in determining taka growth and production. Taka tuber can be an alternative carbohydrate source for supporting food self-sufficiency in coastal communities.
KARAKTERISASI REPRODUKSI DAN NILAI HETEROSIS HASIL PERSILANGAN IKAN GURAME BASTAR DAN BLUESAFIR Deni Radona; Nunak Nafiqoh
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.689

Abstract

Hybridization is one alternative of genetic improvement to increase the production of commercialize commodity. The aim of this study was to determine the character of reproductive performance and the value of heterosis from cross-breeding of giant gouramy (Osphronemus gouramy) strain Bastar and Bluesafir. Crosses were performed in both directions (reciprocal) with naturally spawning system. Two pairs of giant gouramy of each strain were used, each pair contained 4 ? and 1 ?. Every pairs were reared in 3 x 3 m of pond.Reproductive characterization, such as Fecundity, Ovo Somatic Index, egg diameter, egg weight, Fertilization Rate (FR), Hatching Rate (HR), the latency time of hatching, and yolk sack absorbtion period, were observed. Other parameters including larval lenght and Survival Rate (SR) were also observed. Heterosis (H) value was calculated from FR, HR and SR. Result showed that each population and its cross-breed had no different in reproductive characters. Latency time of hatching, egg diameter, egg weight and yolk sac absorbtion period values were 3 ± 0 days; 0.74 ± 0.05 cm; 11 ± 4 mg dan 9 ± 1 days, respectively. Meanwhile fecundity value showed that Blusafir strain had higher fecundity (3661 ± 624), and Ovo Somatic Index percentage (21.96 ± 3.74). The highest HR was presented by ? Bluesafir x ? Bastar (97.95 ± 0.47 %), meanwhile ? Bastar x ? Bluesafir presented the highest FR and SR (99.15 ± 0.34 % and 92.57 ± 0.40 %). Heterosis value showed that cross-breeding between ? Bastar x ? Bluesafir had better HR and SR (1.20 and 2.37), white hand cross-breeding between ? Bluesafir x ? Bastar presented better FR (0.69).
KARAKTERISASI PROTEASE Bacillus subtilis A1 InaCC B398 YANG DIISOLASI DARI TERASI SAMARINDA Yati Sudaryati Soeka; Sulistiani Sulistiani
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.694

Abstract

Proteases is enzyme that breaks the peptide bond to produce amino acids and simpler peptides. This enzyme can be isolated from a variety of sources such as plants, animals and microbe. Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery, food, medicinal formulations and detergents. The objectives of the research was to determine the characteristics of the protease enzyme produced by strain A1, including incubation time, substrate concentration azokasein, the optimum temperature and pH also stability. The effect of some metal ions as activators or inhibitors of the protease enzyme activity measured with a spectrophotometer at ? 280 nm. Strain A1 was identified by using 16S rDNA sequencing and phylogenetic analysis based on Neighbor Joining method. Strain A1 protease activity was qualitatively demonstrated the presence of a clear zone around the colonies in the medium containing 1% skim milk. Result showed that the highest activity were incubation time of three days, temperature of 50 ºC and pH 8.5 were 87.35 U/mL, 83.44 U/mL and 93.11 U/mL, respectively. Effect of metal ions in the form of divalent and monovalent cations at a concentration of 1 mM on protease A1 activated by divalent cations CaCl2, MnCl2 while divalent cations CuCl2, HgCl2 and monovalent cations KCl, NaCl were inhibitors of each enzyme activity. Result from molecular identification based on 16S rDNA sequence and phylogenetic analysis using Neighbor Joining method suggested that strain A1 was Bacillus subtilis. The strain was registered in the InaCC collection (no. B 398).
HUBUNGAN PANJANG DAN BOBOT IKAN NILA LOKAL, BEST F5 DAN F6 DI PANGKEP, SULAWESI SELATAN PADA UMUR 60 HARI PEMELIHARAAN Irin Iriana Kusmini; Rudhy Gustiano; Fera Permata Putri
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.685

Abstract

Best tilapia is breed fish developed by Research Center and Development of Freshwater Aquaculture (BPPBAT) Bogor that has been launched since 2008. Best tilapia is the third generation of gift tilapia. In order to obtain a better growth performance, BPPBAT continued the process of breeding, and to date we have obtained the sixth generations of Best tilapia. This study was aimed to determine growth of tilapia through observation of the relationship between the length and weight of local tilapia, F5 and F6 BEST which were maintained in brackish waters in the Pangkep area, South Sulawesi. Fish samples were taken at random, 30 fishes were taken from each population for length and weight measurement. Calculation of the length-weight of fish in the Pangkep area obtained regression coefficient (b) for local fish 2.4361; BEST F5 3.1077 and F6 3.0522. These values indicated that the local tilapia was negatively allometric while BEST was positively allometric, with local fish condition factor ranged between 0.471-1.334, F5; 0.468-1.479 and 0.498-1.534 F6 respectively. This results has indicating the normal condition of the fish. The length-weight relationship of fish had a value of determinant (R 2) ranged from 0.7829-0.9182, and indicated fish variations influenced by other variables was rather small and has suggested close relationship between length and weight.
VARIASI KARAKTERISTIK PERTUMBUHAN Tacca leontopetaloides (L) Kuntze (Taccaceae) DI PULAU JAWA DAN PULAU-PULAU KECIL SEKITARNYA Fauzia Syarif; Peni Lestari; Albert Husein Wawo
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.690

Abstract

An increasing of population, land conversion, and the behaviour changes in many crops due to global warming could produce a significant negative effect to food security in many countries, including Indonesia. In order to maintain the availability of food, we should find out alternative foods based on corp comodities that can adapt to future agricultural land conditions. One species to be promoted for that purpose is Tacca leontopetaloides (taka). This study was aimed to determine growth characteristics variations of taka in Java and its surrounding small islands through exploration and interview methods. Results indicated that there were two morphological variations of taka based on the canopy colour, namely green and purple. Taka found growing in coastal areas up to100 m above sea level. The light intensity and soil type greatly affected their growth. These plants lived either solitary or in a groups, grow well on sandy soil to clay, under the shade and open areas. Species around taka allegedly contributed in determining taka growth and production. Taka tuber can be an alternative carbohydrate source for supporting food self-sufficiency in coastal communities.
EFEKTIFITAS METODA VAKSINASI FLEXIBACTER PADA BENIH IKAN KERAPU MACAN, Epinephelus fuscoguttatus DI HATCHERI Fris Johnny; Des Roza; Zafran Zafran
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.695

Abstract

Vaccination is one of the approaches against infectious diseases in cultured marine fish. The purpose of this study was to evaluate the effectiveness of Flexibacter vaccine in juvenile of tiger grouper (Epinephelus fuscoguttatus). Nine hundred of tiger grouper with total length of 7-8 cm reared in nine concrete tanks. For each three tanks, fishes were vaccinated with injection, immersion, and without vaccination as control. Booster was delivered at 10 and 30 days after first vaccination. Challenge test with live Flexibacter bacteria was performed 90 days post vaccination. Observations were made on clinical symptoms and survival rates which presented in relative percentage survival(RPS).The experiments were performed in completely randomized design with three replicates. The data then analyzed with ANOVA. The results showed that the antibody titer values of vaccinated fish were higher then unvaccinated fish, namely 128 for injection, 64 for bathing and 4 for control. Survival rates of vaccinated fish following challenged test were higher than control and significantly different (P<0.05), i.e. 88.67% (with RPS of 60%) for injection and 83.33% (with RPS of 50%) for immersion and 66.67% for controls. It is suggested that Flexibacter vaccine administered through injection and bathing are effective to increase immunity of tiger grouper against Flexibacter infection
ANALISIS KERAGAMAN GENETIK KENTANG HITAM Kusumadewi Sri Yulita; Fajarudin Ahmad; Diyah Martanti; Yuyu S. Poerba; Herlina Herlina
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.
POLA INSERSI PARTENOKARPI, DefH9-iaaM PADA GALUR TOMAT TRANSGENIK S.J. Pardal; Slamet Slamet; R. Purnamaningsih; E.G. Lestari
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.691

Abstract

The development of seedless tomato fruits will be more attractive to consumers and industry. Artificial parthenocarpy can be induced through genetic crossing, hormone application or genetic engineering. Development of parthenocarpic tomatos has been done by inserting parthenocarpy gene, DefH9-iaaM into tomato genome via Agrobacterium tumefaciens. Sixty putative transgenic tomato lines were produced, and three events (lines) have been selected as the best event, i.e. OvR1#14-4, OvM2#10-1, OvM2#6-2. These lines contained the DefH9-iaaM based on PCR test. This research aimed was to determine the insertion patern of DefH9-iaaM gene in the progeny of transgenic tomatos lines. Parent variety Oval and line Cl 6046 were used as control plants. Results indicated that tomatos line OvR1#14-4 was still contained the inserted DefH9-iaaM gene and followed the Mendelian pattern (3:1) based on molecular analyses and Chi-square test results, while the others were not identified. Line OvR1#14-4 was required to be further evaluated for phenotypic and genotypic analyses for the expression of their parthenocarpy.

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