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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 26 Documents

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Search results for , issue "Vol 17, No 2 (2018)" : 26 Documents clear

FISIOLOGI PERTUMBUHAN, POTENSI AKTIFITAS PRODUKSI N2O DAN GEN FUNGSIONAL PENYANDINYA PADA BEBERAPA ISOLAT BAKTERI DENITRIFIKASI Agustiyani, Dwi; Laili, Nur; Antonius, Sarjiya
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Physiological characters of four denitrifying bacteria (Bacillus sp. CPNS, Bacillus thuringiensis UPT1, Brevundimonas diminuta EA1 and Bacillus sp. UPSB) were studied based on the growth ability on various nitrate concentrations and the production of N2O gas. The characters of denitrifying bacteria were also evaluated through the existence of functional genes nirS and nosZ, encoding the nitrite reduction and nitrous oxide reduction enzymes which have important role on denitrification processes. The study showed that Bacillus sp. UPSB and Bacillus sp. CPNS isolates have a linear growth with the increasing concentration of KNO3. The N2O gas production of Bacillus sp. UPSB isolate was relatively high, about 70 ?/l, Bacillus sp. CPNS isolate was 25?/l, while the Bacillus thuringiensis UPT1 isolate was 5 ?/l and Brevundimonas diminuta EA1 isolate was 8 ?/l. It was also indicated that both Bacillus sp. UPSB and Bacillus sp. CPNS had high deninitrification activities. It was confirmed that all isolates were contained functional gen of nirS and nosZ.

KERAGAMAN BEBERAPA TUMBUHAN CIPLUKAN (Physalis spp.) DI LERENG GUNUNG KELUD, JAWA TIMUR Hadiyanti, Nugraheni; Supriyadi, Supriyadi; Pardono, Pardono
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Ciplukan (Physalis spp.) is a wild plant which is that potentially used as a medicinal plant. Information related to the diversity of Physalis spp. in Indonesia is limited. The objectives of the research was to identify the diversity of wild Physalis sp. on Mt. Kelud, East Java based on morphological characters, total protein profiles, and flavonoid compounds. The research was conducted by survey on some gradients viz. 200â400, 400â600, 600â800, 800â1,000 and >1,000 m above sea level. Identification of morphological characters was focused on root, stem, leaf, flower and fruit covering 16 characters for scoring. Identification of total protein profile was performed by SDS-PAGE method and the flavonoid compound was analyzed using UV spectrophotometric method based on the colorimetric principle. The degree of diversity of Physalis spp. was determined based on morphological similarity and total protein profiles through cluster analysis. The results showed that Physalis spp. on Kelud was found up to 1,000 m above sea level. Altitude affected to the morphological diversity, total protein profile and the flavonoid compound. Morphological features that play a role in the diversity of Physalis sp. were the direction of growing stems, leaf shape, leaf margin, leaf surface, leaf stalk color, stain color on the inner neck of thr flower, and fruiting calyx color. Expression of total protein P. minima. shows similarity to P. angulata L. Physalis sp. in the low gradient have a higher flavonoid compound as compared to the middle and high gradients. There were two species of Physalis found on the gradient of Mt. Kelud, East Java, namely Physalis minima L. and Physalis angulata L.

PAKET PEMUPUKAN WORTEL PADA TANAH LEMPUNG LIAT BERPASIR DATARAN RENDAH DI PALANGKA RAYA - KALIMANTAN TENGAH Firmansyah, Muhammad Anang; Rahayu, Wiwik; Liana, Twenty
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Carrot (Daucus carota L.) is generally cultivated at uplands areas. However, for lowlands, technology of innovation related to fertilizing can be considered as the solution. This study was aimed to determine the appropriate fertilizer package, the variety of carrot adapted in rainy season, and sandy clay loam of the lowland areas in Palangka Raya, Central Kalimantan. This study was designed by using a split plot exponential design consisting of main plot consisting of carrot varieties: (V1 = Kirana and V2 = Lokal Batu) and sub-plot, consisting of fertilizer packages (P0 = control, P1 = low. P2 = medium, P3 = High). The results showed that variety of Lokal Batu was more adaptive than Kirana for having higher tuber production. 16.95 g/plant (10.12 t/ha) and 16.33 g/plant (9.80 t/ha). The result showed that the highest production of 18.89 g/plant was obtained in a medium fertilizer package as compared to other fertilizer package. There was no significant interaction between carrot varieties and fertilizer packages. The trend showed that the fertilizer packages of P2 produced the highest yield of total weight of tuber production within each carrot variety of 10.36 ton/ha for Kirana and 12.31 ton/ha for Lokal Batu.

PERTUMBUHAN DAN PRODUKSI TEBU (SACCHARUM OFFICINARUM; POACEAE) PADA BERBAGAI PAKET PEMUPUKAN DI LAHAN KERING BERPASIR Supriyadi, Supriyadi; Diana, Nunik Eka; Djumali, Djumali
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Improvement of cultivation technology is a part of the sugarcane intensification programs to supply the national sugar demand. Fertilization package is one component in of the of cultivation technology improvement. The research aims to obtain the information on (1) the role of Ca + Mg versus Sulfur nutrients, (2) the integration of low-dosage compound fertilizers with manure versus high-dosage compound fertilizers without manure, and (3) moderate-dosage versus very high-dosage of compound fertilizer without manure in improving productivity, sugar yield and economic benefits in sandy upland. The research was conducted in November 2012 - October 2013 at Asembagus Experimental Station, Situbondo, East Java. Four fertilizer packages was arranged in a Randomized Block Design and replicated three times. Data were analyzed by orthogonal contrast. The results showed that package of compound fertilizer containing Ca + Mg inccreased productivity (11,67%), sugar yield (15,51%) and economic benefit (293,3%) from the package of fertilizer without containing Ca + Mg with high-dosage Sulfur. Package of high-dosage compound fertilizer containing Ca + Mg without manure inccreased productivity (5.82%), sugar yield (8.26%) and economic benefit (54%) from package of low-dose fertilizer + manure. Package of very high-dosage compound fertilizer containing Ca + Mg inccreased productivity (32%), sugar yield (31,32%) and economic benefit (347,7%) from moderate-dosage fertilizer package.

KARAKTERISASI KERAGAMAN GENETIK 27 GENOTIPE CABAI BERDASARKAN MARKA SSR (SIMPLE SEQUENCE REPEAT) Terryana, Rerenstradika Tizar; Nugroho, Kristianto; Rijzaani, Habib; Lestari, Puji
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i2.3313

Chili pepper (Capsicum annuum) is one of the high economical horticultural comodity in Indonesia and its genetic diversity contributes to the success of breeding programs. Simple sequence repeat (SSR) markers can be used to analyze genetic diversity among chili pepper genotypes. The aim of this research was to analyze the genetic diversity of twenty-seven genotypes of chili pepper by using 24 SSR markers. The collected data was analyzed using cluster analysis and principle coordinate analysis (PCoA). The result showed that high allele variation (4â17 alleles) was observed among chili pepper genotypes tested, with an average allele number and Polymorphism Information Content (PIC) value was 7.708 and 0.758 (0.598â0.920) respectively. All of SSR markers showed PIC value >0.5 which indicated that these markers were suitable for chili pepper diversity studies with a high differentiation and with the average value of genetic diversity was 0.78. The clustering and principle coordinate analysis showed that twenty-seven genotypes of chili pepper were divided into two groups (coefficient of similarity 0.74 in cluster analysis) indicating a high genetic variability among them. Genetic diversity analysis in this study will be useful as an initial basis of selection for appropriate parents with desired traits to assist the breeding program of chili pepper in Indonesia.

MORFOLOGI, ANATOMI DAN UJI HISTOKIMIA RIMPANG Curcuma aeruginosa Roxb; Curcuma longa L. DAN Curcuma heyneana Valeton dan Zijp. Trimanto, Trimanto; Dwiyanti, Dini; Indriyani, Serafinah
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Curcuma is a genus of family Zingiberaceae. Its rhizomes, as well as leaves, have long been used in the traditional medicine. This research aimed to examine the morphological and anatomical structure as well as the primary and secondary metabolites of Curcuma aeruginosa Roxb, Curcuma longa L, and Curcuma heyneana Valeton & Zijp. The Anatomical structure, histochemical test and secretory cell density were observed microscopically. The Histochemical test consisted of amilum, protein, lipid, tanin, alkaloid dan flavonoid tests. Observation of anatomical structures of the of rhizome showed that starch grains has a medium size and shape of starch was oval. Rhizomes of Curcuma longa and C. aeruginosa had a positive correlation for starch, protein, lipids, alkaloids, flavonoids and tannins. C. heyneana has the highest density value on protein while C. longa has the highest density value on lipids, alkaloids, flavonoids and tannins.

KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR) Subositi, Dyah; Widodo, Harto
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5â90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants.Â

HUBUNGAN KARAKTER FENOTIPIK DAN HASIL BIJI PLASMA NUTFAH KACANG TUNGGAK [Vigna unguiculata (L.) Walp.] MENURUT ANALISIS LINTASAN Mastur, Mastur; Setyowati, Mamik; Susilowati, Dwi N.
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Selection of high quality seedling through plant breeding activity can be conducted using yielded seeds, but usually it produces a low heritability. Therefore, this study was aimed to examine and analize several secondary phenotypic characters of 115 cowpea germ plasms in relation to seed yield. Relationship between secondary characters and seed yield was analyzed using path analysis. The result of path analysis showed that the number of cluster and the number of pod exhibited direct and significant effect to the seed yield of cowpea. Therefore, number of cluster and number of pod are necessary to be included as the selection criteria for breeding programme to produce high quality cowpea seedling with high yield.

Pichia pastoris: SEL RAGI UNTUK PRODUKSI PROTEIN REKOMBINAN Herawati, Neng; Kusumawati, Arizah; Santoso, Adi
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pichia pastoris is a group of methylotropic yeast known as a host of expression and protein production which is widely used for biopharmaceutical-based drug production. This yeast can grow fast with a high cell density. Its genetic stability, high cell density, and stress resistance make the development process and scale-up of P. pastoris can increase to a scale of 200,000 liters of culture. In contrast to the expensive and complex development of recombinant protein production in mammalian cells, the development of production in P. pastoris is relatively simple and cheaper. The advantage of P. pastoris as an expression system is that it is able to use methanol as a carbon source by inducing the expression of alcohol oxidase oxidase (AOX) enzyme. Promoter used by this enzyme is also used as a strong promoter for the expression of proteins that we want. Unlike in bacterial and mammalian systems, recombinant protein production in Pichia cells is not contaminated with endotoxins or viruses so it is safer and simplifies the downstream processes in bioproduction. The level of endogenous protein in the low supernatant allows Pichia to cultivate with a high volumetric productivity, therefore the process of protein production becomes very economical. This review provides an overview of several things that must be considered in utilizing P. pastoris as an expression system including the selection of vectors, strains, vector integration mechanisms into the genome, glycosylation processes, and applications in industry.

STUDI ETNOBOTANI JENIS REMPAH YANG DIGUNAKAN DALAM BUMBU MASAKAN TRADISIONAL ADAT DI KERAJAAN ROKAN KABUPATEN ROKAN HULU, RIAU Tribudiarti, Melly; Syamsuardi, Syamsuardi; Nurainas, Nurainas
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Rokan Kingdom was a small kingdom that is now located in Rokan Hulu regency of Riau Province. This region is bordered by North and West Sumatra Province to the north. Rokan Kingdom has a diversity of Indigenous cultures and traditional as an influence of Malay, Minangkabau, Mandailing and Java ethnics. Cultural differences effect people to use variety of herbs as spice in cooking. This study aims to determine the species of plants used as spices in the traditional cuisine within Rokan Palace, Riau and to understand the use value (UV) of each plant. A field survey and interviews were conducted to obtain plant materials and determine its utilization. The voucher specimen stored at Andalas University Herbarium (ANDA), Padang, West Sumatera. Total species obtained in this study is 29 species from 17 families. All of collected plants are used in 16 traditional cuisines. Allium cepa (shallot) has the highest UV = 0.91 as the most common species used as a traditional cooking spice in Rokan Palace.

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