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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 11 Documents
 1   2 
Search results for , issue "Vol 19, No 2 (2020)" : 11 Documents clear
EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN Oom Komala; Novi Fajar Utami; Siti Mariyam Rosdiana
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3697

Abstract

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.
AKTIVITAS ANTIBAKTERI AIR PERASAN DAN REBUSAN DAUN CALINCING (Oxalis corniculata L.) TERHADAP Streptococcus mutans Ni Luh Arisa Prahastuti Winastri; Handa Muliasari; Ernin Hidayati
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3786

Abstract

Inappropriate use of antibiotics can cause bacteria to become resistant and have side effects, therefore an alternative source of antibacterial is needed. One species of plant that has antibacterial properties is calincing (Oxalis corniculata L.). Antibacterial activities of the juice and decoction of calincing leaves against Streptococcus mutans have not reported yet. This study aims to determine the antibacterial activity of the juice and decoction of calincing leaves against Streptococcus mutans. This study uses a well diffusion method with eight treatment groups, namely juice and decoction at the concentrations of 20%, 40%, 60%; listerin 100% as a positive control, and distilled water as a negative control. The diameter of the inhibitory zone of the juice and decoction of calincing leaf at a concentration of 20% are 19 mm and 24.33 mm respectively, and classified as strong growth inhibition, while the concentrations of 40% and 60% are classified as very strong growth inhibition. Growth inhibition of the juice and decoction of calincing leaf at concentration of 20% was significantly higher than the positive control. The best antibacterial activity against Streptococcus mutans is produced by the juice and decoction of calincing leaf at concentration of 60%. Increasing in the concentration of the juice or decoction of calincing leaf also increases the diameter of the inhibitory zone.   
RESPONSIFITAS VARIETAS UNGGUL BARU TEBU MASAK AWAL TERHADAP PEMUPUKAN Mala Murianingrum; Djumali Busro; Prima Diarini Riajaya
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3804

Abstract

More than 40% of the potential gap of varieties of sugarcane with actual results in the field is one of the causes of sugar self-sufficiency in Indonesia which has not been reached. The limited knowledge about the responsiveness of  early maturity sugarcane varieties to ferlization is one of the factors causing the large gap. The study aims to determine the responsiveness of new superior early maturity sugarcane varieties conducted in Janti Village, Kediri during July 2018 – August 2019. The study was arranged in a split plot design and repeated 3 times. The main plot consists of 5 fertilizing doses (0.9; 1.0; 1.1; 1.2, and 1.3 times the recommended dose). The subplots consists of 6 new varieties of early maturity sugarcane (MLG 5, MLG 9, MLG 14, PSMLG 2, PSMLG 1, and PS 881). Regression analysis is used to determine the form of response. The optimum dose was determined using the first derivative for closed quadratic equations and was determined > 1.3 times for positive linear equations. Varieties were grouped based on their optimum dose into five groups (no response, little response, sufficient response, more response, and very responsive). The results showed that the MLG 5, PSMLG 1, and PS 881 were classified as sufficient response, while MLG 9, MLG 14, and PSMLG 2 were classified as very responsive to fertilization. 
JUMLAH, UJI VIABILITAS DAN DAYA KECAMBAH POLEN 31 AKSESI PISANG (Musa sp.) KOLEKSI KEBUN PLASMA NUTFAH PISANG LIPI Erwin Fajar Hasrianda; Ahmad Zaelani; Yuyu Suryasari Poerba
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3859

Abstract

Cross pollination is one of the plant breeding techniques to create a better cultivar with desired traits. A successful cross pollination is affected by quality and quantity of the pollen used. The aim from this study is to know amounts, viability and germination from various banana accessions. This study was conducted by using 31 banana accessions. Pollen quantity per anther, pollen viability, pollen germination growth were measured. The results showed that pollen amounts were ranged between 5.032 and 118.181 pollens per anther. In addition, pollen viability ranged from 30% in Tingalun accession (Musa sp. cv. Tingalun)  to 99% in Malaccensis 2x accession. Furthermore, pollen germination level varied from 1% in Mambee Thu OS accession to 48% in Rutilifes 2x accession. We also found that negative correlation between pollen viability and pollen germination were moderate with correlation coeeficient value of -0,59 ( p-value = 0,00051). From 31 banana accessions tested in this research, pollen source from Malaccensis 2x and Rutilifes 2x banana accessions have the highest potential to be used as the male parent. 
CHROMOSOME COUNT ON YOUNG ANTHER OF BANANA MALE BUD USING EZYMATIC MACERATION AND DAPI STAINING IN SLIDE PREPARATION Fajarudin Ahmad; Yuyu Suryasari Poerba
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3851

Abstract

Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting. 
HUBUNGAN PANJANG-BOBOT DAN FAKTOR KONDISI IKAN NILEM (Osteochilus vittatus VALENCIENNES, 1842) DI PERAIRAN WADUK BENANGA, KALIMANTAN TIMUR Jusmaldi Jusmaldi; Nova Hariani; Nikmahtulhaniah Ayu Wulandari
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3806

Abstract

Biology of bonylip barb (Osteochilus vittatus Valenciennes, 1842) in Benanga Reservoir, East Kalimantan is not yet known. The purpose of this study was to analyze distribution of the length and body weight of fishes, sex ratio, gonad maturity level, length-body weight relationship, and condition factor. In total 278 fishes samples were collected during the five months, from January to May 2019 with a purposive sampling method at Benanga Reservoir, and gill net with  mesh size 0.5; 1; and 1.5  inches used. The results of this research showed that the total length of the fishes analyzed ranged from 72.21–196.57 mm and body weight ranged from 5.12–89.21 g. The sex ratio of male and female were 1:2.02. The male and female fishes were dominated by mature gonad. The length-body weight relationship models calculated for the total fishes sample was W=1x10-5L2.967 (r=0.988) for males, W=5x10-6L3.199 (r=0.989) for females and W=7x10-6L3.126 (r=0.987) for both sexes. Isometric growth patterns (b=3) were observed in males, meanwhile positive allometric growth patterns (b>3) were observed in females and both sexes. Calculation of the condition factor (K) resulted in 1.181±0.117 for males, 0.949±0.094 for females, and 1.026±0.150 for all specimens. The condition factors of bonylip barb from the Benanga Reservoir inside the range recommended as suitable for freshwater fish species in the tropical regions.   
THE DIVERSITY OF BUTTERFLY IN AIR DINGIN LANDFILLS, BALAI GADANG, PADANG CITY Leila Muhelni; Hendra Anwar
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3788

Abstract

The study of butterfly in the in Air Dingin Landfills needs to be carried out to find out what types of butterflies are in this area and how their diversity is, given the increasing area of Landfills that will change the surrounding environment into a place of rubbish heap. The study was conducted by implementing survey method with insect net. The collection of butterflies was carried out in 2 periods (2x6 = 12 days) in April-June 2019 during sunny weather from 09:00 – 12:00 of Western Indonesian Time. All butterflies were collected at ±5 m distance from the collector. The data was analysed to calculate abundance, relative abundance, frequency of the presence of butterfly, and determine diversity was conducted by using the Shannon-Wiener Index and Evenness index. The results obtained 5 families, 14 genera, 22 species, and 220 individuals consisting of 122 males and 98 females. The diversity index value obtained was 2.27 and categorised as the medium category it is mostly because, as assumed, the environmental conditions of the Air Dingin Landfills were still in good condition. 
PENGARUH MEDIA TERKONDISI SEL PUNCA MESENSIMAL TERHADAP EKSPRESI GEN TRANSCRIPTION FACTOR 7-LIKE 2 (TCF7L2) TIKUS MODEL DIABETES MELITUS TIPE 2 Stefani Santi Widhiastuti; Bernadia Branitamahisi; Nor Sri Inayati; Ida Ayu Preharsini; Demas Bayu Handika; Ahmad Hamim Sadewa; Abdurahman Laqif; Sofia Mubarika Haryana
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3830

Abstract

Diabetes mellitus type 2 is the most common type of diabetes. This study was conducted to determine the effect of Mesenchymal Stem Cell-Conditioned Medium (MSC-CM) in Homeostatic Model Assessment of β-cell function (HOMA-β) value, normal Langerhans cells, and Transcription Factor 7-Like 2 (TCF7L2) gene expression in type 2 diabetic rats model. As many as 27 male Sprague Dawley rats were divided into 3 study research groups: normal control (9 normal rats), diabetic control (9 type 2 diabetic rats, induced by 60 mg/kg BW Streptozotocin and 120 mg/kg BW Nicotinamide i.p.), and treatment (9 type 2 diabetic rats treatment with 0.1 ml/200g BW MSC-CM i.p.). On day 30 after therapy, the expression of TCF7L2 gene was performed with real time-quantitative PCR (RT-qPCR). The HOMA-β value were calculated based on Fasting Insulins (FINs) levels and Fasting Blood Glucose (FBG) levels data from other research team members. Based on results, MSC-CM increases the HOMA-β value and amount of normal Langerhans cells of treatment group that indicates amelioration effect of MSC-CM, but there was no significant difference in TCF7L2 gene expression level between diabetic control and treatment group.  
Rafflesia pricei MEIJER (RAFFLESIACEAE): A NEW LOCALITY IN BORNEO Dewi Lestari; Ridha Mahyuni; Rajif Iryadi
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3856

Abstract

Rafflesia pricei is an endemic species from Borneo. Previously, this species had been reported from Sabah, Sarawak, Brunei Darussalam, East Kalimantan (Now, North Kalimantan). A new locality of the R. pricei was found in Pa’ Pulid forest, near to Pa’ Api village, Krayan Nunukan, North Kalimantan. Most vegetation in this area is dominated by Saurauia sp. (Actinidaceae), Knema sp.(Myristicaceae), Arecaceae, Fern, Zingiber sp. (Zingiberaceae) and Araceae. The soil in the area has a brown color, the pH is 6, the texture is dominated by clay (54%), and the soil moisture 80%. 
ISOLASI DAN UJI KOMPATIBILITAS BAKTERI HIDROLITIK DARI TANAH TEMPAT PEMROSESAN AKHIR TALANGAGUNG, KABUPATEN MALANG Prilya Dewi Fitriasari; Nanda Amalia; Susiyanti Farkhiyah
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3828

Abstract

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.   

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