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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 32 Documents
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Search results for , issue "Vol 4, No 5 (1999)" : 32 Documents clear
GAMBARAN MORFOLOGI BUAH/BIJI PERUD PUSPA (Balanophora fungosa J.R. & G. Forst. ssp. indica (Arn.) B. Hansen var. globosa (Jungh.) B. Hansen) Sunaryo Sunaryo
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1253

Abstract

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ISOLAT-ISOLAT KHAMIR DARI MINUMAN TRADISIONAL LARU DI NTT M Rahmansyah; A Kanti
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1244

Abstract

Sixteen isolates of yeast were collected from Law fa fermented palm sap or syrup which were tapped from palm infbrescence stalk of Borassus flabellifer L). Physiological performances were tested according to Bamett method for assimilation of some sources of carbon, nitrogen and cycloheximide. Based on the percentage of discrepancy, 6 isolates have been identified. The isolates were respected in high analogical affinity and identified as Arxula adeninovoras (93%), Lipomyces tetraspows (100%), Rhodotorula mucilaginosa (97%), Saccharomyces cerevisae (95%), Schwanniomyces occidentalis var. occidentalis (96%) and Pichia anomala (99%). The yeast that usually found in food fermentation was P. anomala, R. mucilaginosa and S. cerevisiae. The others yeast have great opportunity as indigenous ones.
KARAKTERISASI AKTIVITAS ANTIMIKROBA BEBERAPA ASAM LEMAK ASKORBIL Elidar Naiola
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1249

Abstract

The study is directed t investigate the antimicrobial activity f six ascorbyl fatty acids. The minimum concentrations were observed in ascorbyl caprate and ascorbyl laurate. Ascorbyl caprate was the strongest ester with the minimum inhibitory concentration (1,25-5 nM). Among microorganisms tested,S. cerevisiae was more senseitive than others. The sensitivity of S. cerevisiae was depending on the strains and S.cerevisiae OUT7054 was the most sensitive strain to the ascorbyl caprate which was the sterilizing agent rather than microbiostic agent.The antimicrobial activity of ascorbyl caprate was infuenced remarkably by temperature and pH.The most effective conditbns for sierilizatin of yeast were higher temperature and low pH.
ANALISIS VARIASI GENETIK Saccharomyces cerevisiae Dl TAHAN ETANOL DENGAN RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Heddy Julistiono; Titin Yulineri; Sukamto Hanjono
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1240

Abstract

Genetic variations among 3 cultures, which were treated with or without Mn of Saccharomvces cerevisiae D1, were analyzed using RAPD (Random Amplified Polymorphic DNA ) technique. The Mn-treatment of three cultures were as follows: the culture KMn was D1 strain, the culture Mn+ was D1 strain colony survived in ethanol 20%, which was previously treated with 0,5 mU MnSOt and the culture Mn- was a D1 colony survived in ethanol 20% without MnSOi treatment. Polymorphism of total DNA of the three cultures may indicate that mutation may occur in cells which were tolerant to ethanol. The locus or base change was not identified. However, since the oxygen uptake rate of the three cultures at catabolite derepression state were identical,the results suggest that the locus may not be in mitochondrial DNA encoding respiratory chain proteins. The relation between DNA polimorphic and ethanol tolerant cell is still to be clarified.
PEMANFAATAN KUSKUS OLEH MASYARAKAT PEDALAMAN IRIAN JAYA W Rosa Farida; Gono Semiadi; Wirdatcti Wirdateti
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1254

Abstract

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REGENERASI TANAMAN DARI JARINGAN KOTILEDON +RUAS BENIH KEDELAI, {Glycine max L., KULTIVAR WILIS) DENGAN RADIASI SINAR GAMMA Dameria Hutabarat; Rivaie Ratma
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1245

Abstract

Soybean Wilis cultivar was efficiently regenerated in vitro via somatic embryogenesis. Cotyledonary explants were excised from mature geminating seeds. Seeds were germinated on agar solution and on B5 medium enriched with 5 ppm BA, 0,25 pom IBA and 500 ppm casein hydrolyzate. Cotyledonary nodes from both germinating seeds were excised and cultured on B5 medium enriched with 5 ppm BA, 0,25 ppm IBA and 500 ppm casein hydrolyzate. Age of seedlings had a remarkable influence on shoot regeneration. Cotyledon from seeds germinated on agar solution with light gave better result in shoot regeneration compare with those germinated in darkness. The highest number of regenerants per explants (5 shoots) was produced by cotyledon from seeds germinated on 65 medium enriched with 5 PPM BA, 0,25 PPM IBA and 500 PPM casein hydrolyzate in darkness. The seeds of soybean were exposed to gamma-rays doses 10 Gy then germinated on S5 medium enriched with 5 PPM BA,0,25 PPM IBA and 500 ppmcase/n hydrolyzate did not improve the number of plant regeneration.Only 5-day-old seedlings from seeds were exposed to gamma-rays dose 30 Gy could improve the number of shoot regeneration, one of the cotyledonary node treated produced 21 regeneration shoots.
PENGUJIAN PERTUMBUHAN DAN DAYA HASIL BEBERAPA GALUR TANAMAN CABE MERAH {Capsicum annuunft.) HASIL PERLAKUAN COLCHICINE Joko R Witono
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1250

Abstract

This experiment aims to examine first generation (F1) of some lines of red pepper (taosicum ann'uum L) resulted by colchicine treatments,the examinatton covering growth and yield characters.The method was Randomized Complete Block Design (RCBD) with 16 lines resulted generation combination soaking time in colchicine solution (0, A 16, and 24 hours) and the red pepper varieties (Cipanas, Bengkulu, Jatilaba, and TIT super). The best growth character is 65, and the best yield character isG16.High heritability characters (height of plant, age of blossom, length of fruit, diameter of fruit, and weight of fruit) could be chosen for selection and plant breeding programs.
KARAKTER MORFOLOGI DAN POLA PERKECAMBAHAN BIJI Strombosia javanica Bl. (Olacaceae) DALAM KAITANNYA DENGAN SIFAT-SIFAT PARASITISME Erlin Rachman; Sunaryo Sunaryo
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1241

Abstract

A study on seed germinations of Strombosia javanica BL. was conducted to observe morphological character and germination pattern of the seedling. As many as 134 seeds of the species, originally collected in Bogor Botanical Gardens, were germinated on medium contain soil, sands and compost in same quantity. Germination aspects such as germination patterns and developmental changes were monitored everyday until the first leaves appear. The seed germinations was typically hypogeal and totally spent 160-170 days, and consisted at least six stages: hypocotyl growth, radix primordial formatton, seedling root growth, seed ball lifting, seed ball removal and first leaves appearance. Rather intact seed ball remain exist at the end of hypocotyl vigorously mtil its removal, pmsumably one of special phenomena of the species germination associated to plant parasitism. Functionally, development of the cotyledons were abnormally suppressed by the existence of the seed ball. The cotyledon praphotosynthetic function seems to be replaced by greeny hypocotyl. First leaves development were never occurred before the seed ball removal. Seed coat elimination was able to increase germination initiation in a given time but facilitated damaging bacteria or fungi to contaminate the endosperm.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) Joko Sulistyo; Yati Sudaryati Soeka; Evi Triana; Rostiati NR Napitupulu
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
KOMPOSISI MINYAK ATSIRI DARI TIGA JENIS TUMBUHAN RUTACEAE Andria Agusta; Yuliasri Jamal
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1251

Abstract

Rutaceae is one of the essential oil resources that is very potential for medicinal, perfumery and other use This study was conducted to analyze the essential oil components resulted from water distillation of kalamanjarik leaves (Micromelum minutum), jeruk \epara leaves ILimnocitrus litoralis) and the leaves and the stem bark of malapotung (Evodia qlabra). The results showed the leaves of kalamanjarik contained approximately 0,70%, jeruk jepara 0,21%, malapotung leaves 0,17% and the stem bark contained 0,50 % essential oils. GCMS analyses of the essential oils gave 3 major components of kalamanjarik leaves i.e /J-pinene (24,41%j, bergamiol (23,01%) and nerolidol (18,90%) and 2 major components of jeruk jepara leaves i.e fl-mircene (70.55) and /t-pinene (24,06 %). Essential oil of malapotung leaves consisted of 39,8J% ct-pinene, 14,26 % 1aR(1aa,4a/3,7a,7aa,7ba)decahydro -1,1,7-lrimethyl-4-methylene-1H-cycloprope azulene and 16,62% S-cadinene as major components, while the stem bark had only one major component, u-pinene (68,37 %) The minor components of the four essential oils will be discussed.

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