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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 16   17   18   19   20 
Struktur Genetik Populasi Burung Betet Jawa (Psittacula alexandri alexandri) Berdasarkan Sekuen DNA Mitokondria Gen ND2 Astuti, Dwi
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3101

Abstract

ABSTRACTThis study was aimed to determine the structure and genetic characteristics of the Javan Parakeets (Psittacula alexandri alexandri) in some populations in Java Island based on ND2 gene sequences. This study used 26 birds composed of 9 birds from Bogor (West Java), 8 birds from Cepu/Blora (Central Java), 5 birds from Yogyakarta (DIY), and 4 birds from Ngawi (East Java). Blood samples were taken from each bird and DNA was extracted, and then a single fragment of ND2 gene was PCR amplified. A totaly of 1020-bp of DNA sequence of all individual birds were analyzed and produced low genetic distance between bird populations: 0.0031 ± 0.0010 (0.0024 ± 0.0012 to 0.0058 ± 0.0022). The highest genetic distances among individual bird were found in the Yogya’s population followed and were lowest in Cepu/Blora’s population. There were 16 haplotype sequences (Hpa Hpa1-16) found in 26 birds. There were some identical haplotypes in each population, but each population has different haplotypes from each other. The highest haplotype diversity and nucleotida diversity were found in Yogya’s and Bogor populations. NJ tree showed that each individual bird clustered together corresponded to the population/progeny.Keywords: Javan Parakeets, Psittacula alexandri alexandri, population, ND2 gene sequence genetic distance, diversity
Kinetic Parameters of Endochitinase from Bacillus licheniformis MB-2 Toharisman, Aris; Barth, M. Spindier; Suhartono, T.
JURNAL BIOLOGI INDONESIA Vol 4, No 2 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i2.3265

Abstract

ABSTRAKParameter Kinetika Endokitinase dari Bacillus licheniformis MB-2. Bacilluslicheniformis MB-2 yang diisolasi dari air panas di Tompaso (Sulawesi Utara)menghasilkan 5 enzim kitinase ekstraselular. Salah satu enzim, Chi-67, telah dimumikandan diarekterisasi. Chi-67 dapat menghidrolisis substrat kitin buatan maupun alami.Analisis kinetika aktivitas enzim terhadap berbagai substrat kitin telah dilakukan. Enzimdapat memotong 4-methylumbelliferyl N, N-diacetylchitobioside {MUF(GINAc),}, 4-methylumbellifelyl N:N N-triacetylchitotrioside {MUF(GINAc),), glikol kitin, dankoloidal kitin, tetapi tidak dapat melepaskan senyawa MUF dari 4-methylumbelliferylN-acetyl-R-D-glucosamine {MUF(GINAc)}. Ini menunjukkan Chi-67 memiliki tipepernotongan endo. K u ~kaec epatan reaksi substrat menunjukkan pola profil Michaelis-Menten. Kecepatan reaksi maksimum (V,,) terhadap MUF(GlNAc), identik denganVmatxe rhadap MUF(GINAC)~ya itu 0.02 pM.mitil . VmaeXn zim terhadap koloidal kitinadalah 0.01 pM.h- atau lebih rendah 3 kali dari nilai VmaXpa da substrat glikol kitin (0.03M h ) Nilai Kcu, untuk koloidal kitin sebesar 8.01 h- atau hampir 2 kali lebih tinggidari glikol kitin (4.32 h-I) sementara Kc, untuk MUF(GlNAc),adalah 3.65 min- atau1,5 kali lebih tinggi dari Kc%,M UF(GINAc), yang hanya 2.90 min- . Afinitas Chi-67terhadap koloidal kitin, glikol kitin, MUF(GINAc),, dan MUF(GINAc), yang ditunjukkanoleh nilai konstanta Michaelis (K,) masing-masing 3.08 rng.ml-I, 0.32 mg.ml-I, 0.26 mMdan 0.1 mM.Kata kunci: Bakteri, endokitinase, enzim, dan stabilitas panas
Komposisi Floristik dan Struktur Hutan di Pulau Natuna Besar, Kepulaun Natuna Mirmanto, Edi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2100

Abstract

Ecological study has been conducted in the forest area of Natuna Island to reveal the structure and composition oftree species. In the 10 plots of 20 x 40 m each, there were 141 species of trees, with a density of 504 trees / ha and12.35 m2/ha of basal area, which was distributed in three forest types. Myrtaceae was the most common family,followed by Euphorbiaceae and Dipterocarpaceae. On the other hand Arthrophyllum javanicum recorded as themost common species together with Syzygium aemum and Ilex pleiobrachiata. The existence of each of the common speciesvaries in each forest type that shows there was a correlation between the condition of the habitat and the presence ofspecies.Keywords: floristic composition, structure, the Natuna Besar, Riau
Profil Mamalia Kecil Gunung Slamet Jawa Tengah Maharadatunkamsi, Maharadatunkamsi
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.108 KB) | DOI: 10.14203/jbi.v7i1.3137

Abstract

ABSTRACTProfile of Small mammals from Gunung Slamet, Central Jawa. Research of small mammals atGunung Slamet, Central Jawa was conducted. Three types of habitats as representation ofprimary forest, secondary forest and plantation were examined at Kalipagu, Kaliwadas andBambangan in order to record its small mammals biodiversity. Combination of trapping anddirect observation recorded 31 species of small mammals from the areas observed. Shannon-Wiener index was the highest in secondary forest (3.8) compared to primary forest (3.6) andplantation (3.4). The results indicated that most small mammals of Gunung Slamet use secondaryforest for their home range. However data on microchiropterans that were more frequentlyrecorded in plantations indicated that agricultural plants were their primary foraging areas. Onthe other hand, most of small mammals species were rely on both secondary forest and primaryforest as an important food resources. The dendogram resulting from cluster analysis wasconcordant with this view. Primary forest and secondary forest clustered closely together,while plantation was recognized as a distinct group.Key words: Small mammals, Gunung Slamet, biodiversity, habitat.
Peningkatan Pertumbuhan Kultur Tunas Stevia rebaudiana Bertoni pada Media dengan Peningkatan Kadar Vitamin dan Glisin serta Penggunaan Jenis Tutup Tabung Berbeda Ermayanti, Tri Muji; Rantau, Deritha Elly; Hafiizh, Erwin Al; Maulana, Evan
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2836.935 KB) | DOI: 10.14203/jbi.v13i2.3395

Abstract

ABSTRACTStevia rebaudiana is plant species producing natural sweetener with has low calories. The species propagation could be done by tissue culture tecnique to obtain propagules with high quality and sustainability. Modification of media composition and in vitro environment will increase growth and vigority of explants so that they have high survival rate during acclimatization. The aim of this research was to increase growth of stevia shoot culture by increasing the level of vitamins in combination with different type of test tube covers. Stevia shoot tips were cultured on MS medium containing normal concentration of its vitamins (control treatment; Myo-inositol 100 mg/l; Nicotinic acid 0.5 mg/l; Pyridoxine-HCl 0.5 mg/l; Thiamine-HCl 0.5 mg/l and Glycine 2 mg/l), twice and 4 folds of vitamin levels, they were grown on culture tubes with Al-foil and ventilated-plastic with filter (2 cm diameter and pore size at 0,22 micron). Height of shoots, number of nodes, number of leaves, number of roots were observed every week till 8 weeks of culture. Biomass (fresh and dry weights) and chlorophyll level and acclimatization were done 8 weeks of culture. The results showed that type of culture tube covers affected significantly to all growth parameters, biomass as well as level of chlorophyll, meanwhile level of vitamins only affected number of nodes, shoots and roots. Interaction between vitamin level and covers types only occured for height of shoots and number of roots. Plantlets grown on medium containing 4 fold of vitamin level (Myo-inositol 400 mg/l; Nicotinic acid 2 mg/l; Pyridoxine-HCl 2 mg/l; Thiamine-HCl 2 mg/l and Glycine 8 mg/l) with ventilated-plastic cover had larger leaves compared to other treatments. All plantlets survived in a greenhouse.Keywords: Stevia rebaudiana, in vitro growth, increase in vitamin concentration.
Stok Karbon dan Biomasa Beberapa Komoditas Tanaman Pertanian Di Bodogol- Taman Nasional Gunung Gede Pangrango – Jawa Barat Rahajoe, Joeni Setijo; Alhamd, Laode; Handayani, Dewi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2886

Abstract

ABSTRACTThe study of carbon stock and biomasa of agricultural commodities was conducted in the Bodogol village, Gunung Gede Pangrango National Park - West Java. The purpose of this research to determine the biomasa, carbon stock and the rate of decomposition of agricultural commodities by using destructive sample and litterbag method. Six of agricultural commodities: green beans/buncis (Phaseolus vulgaris), chilli (Capsicum annum), corn (Zea mays), bean (Vigna cylindrica), peanuts (Arachis hypogaea) and cassava (Manihot esculenta), were calculated their biomasa and carbon stocks. The result showed that the biomasa of agriculture commodities in the range of 0.152 to 4.216 t ha-1, with a carbon stock ranging from 0.01 to 1.83 t ha-1. The decomposition rate (k) of those commodities were k = 5.6 y-1; 5.48 y-1, 5.18 y-1, 5.04 y-1, 4.42 y-1, and k = 1.21 y-1, for Manihot esculenta, Vigna cylindrica, Arachis hypogea, Zea mays, Capsicum annum and Phaseolus vulgaris, respectively.Keywords: Biomasa, Gede Pangrango, Carbon, Agricultural commodities
Deforestation and it is Implications for Sumatran tigers in Bukit Barisan Selatan National Park, Sumatra Suyadi, Suyadi; Jaya, I Nengah Surati; Wijanarto, Antonius B.; Wibisono, Haryo Tabah
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.119 KB) | DOI: 10.14203/jbi.v9i1.148

Abstract

Taman Nasional Bukit Barisan Selatan (TNBBS) memiliki ekosistem yang lengkap mulai dari hutan dataran rendahtermasuk hutan mangrove hingga hutan dataran tinggi. Taman nasional ini teridentifikasi sebagai area konservasiprioritas utama untuk harimau sumatra (Tiger Conservation Unit 1). Akan tetapi, banyak tutupan hutan yang digunduli (deforestasi). Deforestasi bukan hanya menjadi ancaman besar bagi kelestarian hutan tetapi juga terhadappopulasi harimau sumatra. Penelitian ini menghubungkan data citra satelit Landsat dengan data survei harimaumenggunakan kamera otomatis (camera traps) untuk mengetahui pola deforestasi, populasi harimau, dan implikasideforestasi terhadap populasi harimau. Hasil penelitian menunjukan sekitar 27% tutupan hutan di TNBBS telahhilang. Rata-rata laju deforestasi dalam satu dasawarsa terakhir (1998 - 2006) sebesar 18,76 km2/tahun. Jumlahharimau menurun dari 57 individu pada tahun 1998 menjadi 22 individu di tahun 2006, dan kepadatan populasinyajuga menurun dari 2,25 individu/100km2 menjadi 1.16 individu/100km2. Harimau di TNBBS memilikihabitat yang bervariasi mulai dari hutan dataran rendah termasuk mangrove ikutan hingga dataran tinggi.Kata Kunci: Deforestasi, Mangrove, Camera Trap, Citra Satelit, Harimau Sumatra, Taman Nasional Bukit BarisanSelatan
Pengaruh Mikoriza Arbuskula dan Pupuk Fosfat Terhadap Pertumbuhan Jambu Mente pada Tanah Podsolik Merah Kuning Trisilawati, Octivia; Supriatun, Titin; Indrawati, Ida
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.958 KB) | DOI: 10.14203/jbi.v3i2.3476

Abstract

ABSTRACTEffect of Arbuscular Mycorrhizae Fungi and Phosphate Fertilizer to the Growth of Cashew on Red Yellow Podzolic Soil. The research about the effect of arbuscular mycorrhizae (AM) fungi and phosphate fertilizer applications to the growth of cashew seedling (Anacardium occidentale L.) grown on the red yellow podzolic soil was conducted in the green house at Cimanggu Research Installation, Research Institute for Spice and Medicinal Crops, Bogor. Completelly randomized design with factorial pattern, two factors and three replications was used. First factor was AM inoculation consisted of: without AM, Glomus etunicatum, Mycofer, whether second factor was phosphate fertilizer level consisted of: 0, 15, 30, and 45 g P2O5 per plant. The result showed an interaction between AM and phosphate fertilizer levels to the plant height, leaves dry weight, and spore population, and the best result on giving mycofer with P level 30 g P2O5/plant. AM inoculation significantly effected plant height, stem diameter, dry weight of leaves, stem and root, percentage of AM infection, and spore population, whether phosphate application significantly effected all of the parameters.Key words : Arbuscular mycorrhizae, phosphate, Anacardium occidentale L., plant growth
Anthropogenic Influences on The Sosioecology of Long-Tailed Macaques (Macaca fascicularis) in Lombok Island, Indonesia Hadi, Islamul; Suryobroto, Bambang; Watanabe, Kunio
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.427 KB) | DOI: 10.14203/jbi.v8i1.3061

Abstract

Beberapa Pengaruh Antropogenik pada Sosioekologi pada Monyet ekor Panjang (Macaca fascicularis) di Pulau Lombok, Indonesia. Survey distribusi monyet ekor panjang dilakukan di P. Lombok selama 2001-2009 dan dari 37 kelompok, satu kelompok jantan muda dan 3 individu soliter yang terdata dari 27 lokasi sekitar 63% monyet dijumpai pada areal karena terpengaruh oleh aktivitaskehidupan manusia, seperti hutan sekunder, kebun buah-buahan, hutan persembahan dan tempat rekreasi. Besarnya kelompok monyet cenderung menjadi lebih besar di area semi buatan dibandingkan dengan kelompok yang hidup liar (Liar= 7.08, buatan =19.04, X2 = 5.4763, df = 1, P= 0.01928). Faktor-faktor ketergantungan pada manusia yang menyediakan sejumlah pakan ternyata mempengaruhi tingkah lakunya sedangkan penebangan hutan dan pengubahan alih fungsi menjadi kawasan lain selain hutan akan memicu monyet terkesan menjadi hama tanaman.Kata Kunci: Monyet kra, Macaca fascicularis, Pulau Lombok, Anthropogenik
Pemetaan Genetik Virus Avian Influenza di Indonesia 2007 Dharmayanti, NLP Indi; Indriani, R.; Hartawan, R.; Hewajuli, D.A.; Ratnawati, A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.831 KB) | DOI: 10.14203/jbi.v5i2.3195

Abstract

ABSTRACTGenetic Mapping of Indonesian Avian Influenza Viruses 2007.Department of agricultureimplements vaccination as one a tool to control of avian influenza disease. The vaccinationprogram use virus seed such as H5N1, H5N2 and H5N9. Such as vaccination program for otherdiseases, avian influenza vaccine program have lack implementation in the field. In 2007,department of agriculture evaluated the AI vaccination program such as the master seedvaccine that can be used. Result of the evaluation showed that 11 of AI vaccines that weredistributed in Indonesia did not give protection more than 60% to Indonesian isolates in 2006(A/Ck/Pwt-Wij/06). From this point and many AI cases in the field in 2007, the aim of thisstudy was to conduct genetic diversity of avian influenza viruses which have circulated inIndonesia region. We used virus isolation for propagate the viruses, RT-PCR for identificationand DNA sequencing on HA1 region to analysis genetic diversity for genetic mapping anduseful for master seed candidate. The result of the study showed that there were 6 group ofgenetic diversity in 2007; Isolates from group 1, 5 and 6 can be used for AI vaccine candidate.Key words: genetic mapping, genetic diversity and avian influenza viruses

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