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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
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Penampilan Karakter Kuantitatif Genotipe Kedelai di Bawah Naungan Sundari, Titik; Wahyuningsih, Sri
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1046.565 KB) | DOI: 10.14203/jbi.v13i1.3103

Abstract

ABSTRACTSunlight is main source of energy in photosynthesis process. In any habitat, light intensity varies temporally and spatially. Plants develop the strength of acclimatization and plasticity to overcome these problems. Each plant provides a different response to shade, and expressed through diverse appearance of the characters. This research aims to know the ability of soybean genotypes in the response to shade. Research conducted at Kendalpayak Research Station, Malang in 2013. The research used factorial experimental design, with two factors. The first factor is shade, consisted of without shading (N0) and 50% shading (N1). The second factor is soybean genotypes, consisting of three sensitive varieties to shade (Grobogan, Argopuro, and Panderman), two resistant varieties (Dena1 and Dena 2), and two lines of crossbreeding (IBM-10-75 and K-13). Placement of treatments was based on randomized complete block design, repeated three times. Observations included: plant height, leaves number, leaf area, stem diameter, seed weight per plant, 100-seed weight and dry weight: total, roots, stems, leaves and pods. The results showed that the treatment of 50% shade causes the low light stress, with intensity of 30.31%. That stress intensity, causes differences in the quantitative characters performance among genotypes tested. Dena 1, Dena 2, and K-13 suitable for shade environment, being able to maintain or increase the seed yield, whereas Argopuro, IBM-10-75, Grobogan, and Panderman not suitable for shade environment, because not able to maintain the yields. Dena 1 and Dena 2 developed avoidance mechanisms to respond shade, through increase in plant height, number and leaf area, while K-13 respond to the shade stress, through its ability to utilize absorbed light for seed formation an efficient.Keywords: stress, low light, soybeans
Distribusi Bakteri Nitrifikasi di Danau Paparan Banjir Studi Kasus : di Suaka Perikanan Danau Loa Kang, Kalimantan Timur Badjoeri, Muhammad
JURNAL BIOLOGI INDONESIA Vol 4, No 2 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4136.284 KB) | DOI: 10.14203/jbi.v4i2.3267

Abstract

ABSTRACTDistribution of Nitrifying Bacteria in Floodplain Lake. Case Study in Lake LoaKang Fishery Reserve, East Kalimantan. Lake Loa Kang is a fishery reserve locatedin a floodplain system of River Mahakam. This floodplain lakes consists of severalhabitat types among the others are backswamp deposit lakes represented by Lake LoaKang and Lake Balikpapan. This two floodplain lakes exchange water with RiverMahakam and one of its tributary, the River Pela. Nitrification is one key ofmicrobiological process of nitrogen cycle in inland water. The nitrifying bacteria functionalgroup that mediated the process can be detected and identified by enrichment culture.This study was aimed to reveal feature of nitrification process in several floodplainhabitat types of Lake Loa Kang Fishery Reserve (LLFR) system with emphasize on thespatial distribution pattern of nitrifying bacteria. The results ofthe study will be utilizedas limnological database for development of conservation and restoration of Pesut habitat.Water samplings are conducted for three times in 2003, those were in dry season (June),transitional (August) and the rainy (Sept.). Results of the study showed that the numberof nitrifying bacteria is highest at connecting channel between floodplain lakes (L. BalikPapan) and River Mahakam and the lowest in small size ephemeral pond in LLFR (Lopak).Ex situ simulation of this biogeochemical cycle indicate a duration of 13 - 21 days tocomplete the nitrification process. The number of nitritation and nitratation bacteria inLake Loa Kang in the dry season is smaller compared to the number in rainy season. Theduration of nitrification process in LLFR suspected to be influenced by the number ofbacteria, organic material and season.Key words: Distribution,Floodplain, Lake Loa Kang Fishery Reserve, Nitrifying bacteria
Zingiberaceae of the Ternate Island: Almost A Hundread Years After Beguin’s Collection Andriyani, Marlina
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3139

Abstract

ABSTRAKZingiberacea Pulau Ternate: Hampir Seratus Tahun Koleksi Beguin. Ditemukan sepuluh jenis Zingiberaceae yang mewakili lima marga (Alpinia, Etlingera, Hornstedtia, Globba, Boesenbergia) di Pulau Ternate. Alpinia novae-pommeraniae K. Schum. dan A. pubiflora (Benth.) K. Schum. merupakan catatan baru untuk Maluku. Pengkoleksian kembali Alpinia regia dari lokasi tipe memberikan tambahan informasi baru di mana spesimen tipe (Beguin1234 di herbarium L) tidak lengkap. Kata kunci: jahe-jahean, Maluku, Pulau Ternate, Beguin.
Senyawa Antibakteri yang Diproduksi oleh Lactobacillus plantarum dan Aplikasinya untuk Pengawetan Bahan Ikan Sulistiani, Sulistiani
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (962.606 KB) | DOI: 10.14203/jbi.v13i2.3397

Abstract

ABSTRACTLactic acid bacteria (LAB) generally accepted as safe microorganism and play an important role in the fermen-tation and preservation of food/feed. LAB is known to extend the shelf life of food/feed, because of its ability to produce antibacterial compounds such as lactic acid, hydrogen peroxide and bacteriocins. The objective of this study was antibacterial compounds produced by Lactobacillus plantarum and their application for fish preserva-tive. The research results showed L. plantarum [Su-ls520, Su-ls530, Su-ls537] produces lactic acid from 71.16 to 77.91mg/mL, hydrogen peroxide from 0.17 to 0.25 mg/mL and producing bacteriocins indicated by the pres-ence of the gene encoding plantaricin, an antibacterial compounds that act as food preservative. Lactic acid 1.5% and 3% salt was not able to suppress the growth of bacteria, while fermentation solution (supernatant) of L. plantarum [Su-ls520, Su-ls530], chitosan 1.5% and a mixture of lactic acid 1.5% and salt 3% slightly sup-press the growth of spoilage bacteria on the fish material stored 48 hours at room temperature. Treatment with fermentation solution (supernatant) of L. plantarum Su-ls537 able to suppress strongly growth of spoilage bac-teria in fish material for 24 and 48 hours storage. At 72 hours of storage the fish material rotted, the addition of preservatives no longer able to suppress the growth of spoilage bacteria.Keywords: lactic acid bacteria (LAB), Lactobacillus plantarum, fish, antibacterial compounds, preservation
Pengembangan Identitas Spesifik Berbasis Marka SSR pada 29 Varietas Kedelai Lokal Indonesia Lestari, Puji; Risliawati, Andari; Utami, Dwinita Wikan; Hidayatun, Nurul; Santoso, Tri Joko; Chaerani, Chaerani
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.015 KB) | DOI: 10.14203/jbi.v12i2.2888

Abstract

ABSTRACTSoybean is an important legume crop in Indonesia and local varieties from different regions are diversed. Accurate identification of local varieties is needed to determine the genetic identity in order to protect them to indicate the geographical origin and registration purpose. This study aimed to analyze DNA fingerprints of local varieties of soybean in Indonesia to develop a specific identity using SSR markers along with the marker set. A total of 29 local varieties/accessions was analyzed using 9 selected SSR markers with semi automated genetic analyzer on the basis of capillary electrophoresis. Total markers successfully detected 50 alleles in all varieties, with the average polymorphism information content (PIC) of each locus (0.579) and the genetic diversity index (0.629) were quite high as a reflection of diversity of the local varieties. Based on the analysis of the genetic diversity index, PIC, rare alleles, dominant allele frequency, analysis of genetic distance, 6 SSRs (Satt009, Satt038, Satt177, Satt242, Satt308 and Satt114) were selected as a set of markers for variety identification. Although three varieties can not be distinguished because of their close genetic relationship, but the digital coding indicates that local varieties of soybeans have possessed specific identities (ID) representing variation of SSR alleles. The selected SSR loci with additional identifier can be used to develop ID of Indonesian local soybean in the germplasm collection. Overall, the marker set is a powerful tool to complement morphological markers for the protection of local soybean varieties that contribute in accelerating local varieties improvement in the future agriculture.Keywords: specific identity, SSR, Indonesian local soybean variety, DNA fingerprint.
Toksisitas Isolat Bacillus thuringiensis yang Mengandung Gen cry 1A Terhadap Hama Penggerek Batang Jagung, Ostrinia furnacalis Guenee Bahagawati, Bahagawati; Rizjaani, Habib; Sibuea, Agustina K.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3174

Abstract

ABSTRACTCry genes isolated from Bacillus thuringiensis produce crystal proteins that exhibit a highinsecticidal activity against several plant pests. The objectives of this experiment were todetect the presence of cry1A sequences from several local Bacillus thuringiensis isolatesmultiplied by Lep1A-Lep1B and Lep2A-Lep2B primers using PCR technique and to determinetheir toxicity against Ostrinia furnacalis, maize stemborer. From 59 tested isolates, 6 of themgave PCR products, two DNA bands, first was 490 bp, the expected size of Lep1A-Lep1Bprimers, and second was 986 bp, the expected size of Lep2A-Lep2B primers. These isolateswere Jtg 2151, 243, Cib 551, Lam 752, Lam 762 and C 522. All of tested isolates showedpotentially high toxicity against maize stemborer.Key words: B. thuringiensis, cry1A, Ostrinia furnacalis, PCR.Kata kunci: B. thuringiensis, cry1A, Ostrinia furnacalis, PCR.
Karakteristik Populasi Labi-labi Amyda cartilaginea (Boddaert, 1770) yang Tertangkap di Sumatera Selatan Sentosa, Agus Arifin; Wijaya, Danu; Suryandari, Astri
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.164

Abstract

The Asiatic softshell turtle Amyda cartilaginea (Boddaert, 1770) is one of the reptile commodities included in CITESAppendix II with vulnerable status according to IUCN. The species has been harvesting, especially for export purposein South Sumatera. The reseach was aimed to know the population characteristics of the Asiatic softshell turtleharvested in South Sumatera. The study was carried out based on enumerators approach from July to Desember2012 in South Sumatera. The data enumeration also has been collected from the 1st collectors. Data analysis includedthe size distribution of carapace curve length (CCL), carapace curve width (CCW), body weight, sex ratio, agestructure, CCL-weight relationship and von Bertalanffy growth parameters. The results showed that there wererecorded 306 individuals of A. cartilaginea (92% adult) with sex ratio male and female is 42:58. Its has carapacecurve length range from 10 to 75.5 cm, carapace curve width 9 to 59.5 cm and body weight 0.02 to 40 kg. A.cartilaginea growth pattern was negatively allometric (b = 2.727). The von Bertalanffy growth formula of A.cartilaginea in South Sumatera was PLK(t) = 78,75{1-exp[-0,18(t-(-0,72)]} cm.Keywords: Amyda cartilaginea, population characteristics, South Sumatera
Siklus Hidup Jamur Konsumsi Lokal Kulat Kritip (Schizophyllum commune) Pada Daerah Bergambut dan Daerah Bertanah Mineral serta Potensi Nutrisinya Nion, Yanetri Asi; Djaya, Adrianson Agus; Kadie, Evi Marlia; ,, Lune; ,, Sumarlan; Wijaya, C. Hanny
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3060

Abstract

Tulisan Pendek
Deletion Analysis of a Xylanase Gene from Fibrobacter succinogenes S 85 and Its Expression in Escherichia coli HB 101 Suryadi, Y.
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.004 KB) | DOI: 10.14203/jbi.v5i1.3207

Abstract

ABSTRAKAnalisis Delesi Gen Xilanase Asal Fibrobacter succinogenes S 85 dan Ekspresinyapada Escherichia coli HB 101. Plasmid pBX6 dengan sisipan DNA berukuran 3 Kb asalDNA genomik Fibrobacter succinogenes S 85, menyandi aktifitas xilanolitik (mampumendegradasi substrat xilan) bila ditumbuhkan dalam vektor E. coli HB 101. Tujuanpenelitian ini adalah untuk mengidentifikasi lokasi fragmen DNA yang berperan dalammenentukan aktifitas pBX6 menggunakan analisis delesi. Suatu seri analisis delesi terhadappBX6 telah dilakukan dengan menghilangkan beberapa fragmen DNA dengan cara kloninghasil restriksi enzim maupun PCR. Satu fragmen delesi asal produk PCR berukuran 0.7Kb di sub kloning menggunakan cloning kit pCR TOPO dan aktifitas xilanolitiknya ditapislebih lanjut dengan menumbuhkan dalam media agar oat spelt xylan-Remazol brilliantblue (RBB) ditambah antibiotik ampicilin (50 ?g/ml). Transforman positif selanjutnyadiekstraksi dan dikarakterisasi. Hasil penelitian menunjukkan klon yang telah terpotongkehilangan sebagian besar aktifitas xilanolitiknya dibanding klon asal, bila diuji pada kondisiyang sama untuk menghidrolisis substrat xilan. Klon plasmid rekombinan yang telahterpotong menunjukkan aktifitas spesifik sebesar 41,29 + 0,025 U/mg.Key word: Delesi, F. succinogenes S 85; E. coli HB 101, xilanase
Perbandingan Penggunaan Konjugat Antibovine IgG-HRP dan Protein A/G-HRP Dengan Beberapa Larutan Pengencer Serum Pada ELISA Untuk Deteksi Surra pada Sapi dan Kerbau Subekti, Didik T.; Yuniarto, Ichwan
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.292 KB) | DOI: 10.14203/jbi.v13i1.3093

Abstract

ABSTRACTSurra was reported in various types of animals both livestock, pets and wildlife. ELISA (enzyme linked immunosorbentassay) is a sensitive and specific diagnosis technique for Surra detection. The use of speciesspecific conjugate (anti bovine IgG-HRP) has limited while protein A/G can be used for a wide variety of animalspecies. This study aimed to evaluate the initial application of the protein A/G-HRP compared with antibovine IgG-HRP using standard samples using four (4) types of diluent buffer. The standard serum samples (23serum) consist of positive and negative sera from bovine (cattle and buffalo) were reacted with Surra antigenon microplate. Positive and negative serum was diluted with different diluent buffer, namely PBS-Tween20(PBST), RBA (RedBuff A), RBB (RedBuff B) and LC (Low Cross). Results of ELISA using protein A/G-HRPshowed absorbance values reduced 36.16% - 69.30% compared to the anti-bovine IgG-HRP. The percentagereduction of PBST, RBA, RBB and LC, were 51.76%; 56.64%; 36.16% and 69.30% respectively. The use ofprotein A/G-HRP and fourth diluent buffer can reduce antigen - antibody bonding with a weak affinity whichlowers the absorbance value of ELISA Surra.Keywords : Protein A, Protein G, Surra, ELISA, Trypanosoma evansi

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