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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 29   30   31   32   33 
Affect of Canopy Stratum and Methods of Breaking Seed Dormancy on Seedling Growth of Calliandra tetragona Beth. and Acacia tamarindifolia (L.) Willd. Ekasari, Indriani
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.522 KB) | DOI: 10.14203/jbi.v7i2.3085

Abstract

Pengaruh Strata Tajuk Pohon dan Metode Pemecah Dormansi Biji Terhadap Pertumbuhan Semai Kaliandra (Calliandra tetragona Benth.) dan Akasia (Acacia tamarindifolia (L.) Willd.) Fase semai merupakan langkah awal untuk pertumbuhan suatu pohon. Diduga terdapat beberapa faktor yang mempengaruhi pertumbuhan semai yang berasal dari biji. Beberapa faktortersebut antara lain pengaruh penerimaan cahaya matahari terhadap letak buah pada strata tajuk pohon serta metode pemecahan dormansi biji pada tipe biji ortodok menjadi sangat penting untuk diperhatikan. Sedangkan jenis-jenis pohon yang diuji pada studi ini adalah Kaliandra (Calliandra tetragona Benth.) dan Akasia (Acacia tamarindifolia (L.) Willd.). Keduajenis pohon ini merupakan jenis tanaman multi fungsi karena dapat digunakan sebagai tanaman perintis (pioneer species). Tujuan penelitian ini adalah untuk mengetahui pertumbuhan semai yang paling baik dengan menentukan letak buah pada posisi strata tajuk serta menentukanmetode pemecah dormansi biji yang paling tepat untuk biji ortodok. Lokasi penelitian berada di wilayah UPT Balai Konservasi Tumbuhan Kebun Raya Cibodas-LIPI. Pengujian pengambilan buah pada kedua jenis pohon dibagi menjadi 2 strata tajuk (atas dan bawah) dan metode pemecahan dormansi biji dibagi menjadi 3 (kontrol, fisik dan mekanis). Setiap satu jenis pohonterdapat 6 kombinasi perlakuan. Sedangkan setiap kombinasi perlakuan berisikan 100 biji dan hanya 20 semai yang dihitung untuk sample pada tiap kombinasi perlakuan. Penelitian ini dilakukan hingga semai berumur 3 bulan. Parameter pengamatan pertumbuhan semai ini adalah panjang batang dan panjang akar. Hasil ANOVA didapatkan hasil pertumbuhan batang kedua jenis pohon tersebut signifikan (0,026) pada level 5% untuk interaksi jenis pohon, strata tajuk pohon dan metode pemecahan dormansi. Hasil pada pertumbuhan panjang akar terlihat signifikan (4,239) pada level 5% untuk interaksi antara jenis tanaman dan strata tajuk pohon.Sedangkan interaksi antara metode pemecah dormansi dan jenis pohon signifikan (20,332) pada level 1%.Kata kunci: Strata tajuk, Pemecah dormansi biji, Pertumbuhan semai.
Partial Purification and Characterization of Chitin Deacetylase Produced by Bacillus thermoleovorans LW-4-11 Toharisman, Aris; Suhartono, Maggy T.
JURNAL BIOLOGI INDONESIA Vol 4, No 4 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i4.3249

Abstract

ABSTRACTPurifikasi Parsial dan Karakterisasi Enzim Kitin Deasetilase yang Dihasilkan Bacillus thermoleovorans LW-4-11. Kitin deasetilase yang dihasilkan dari bakteri Bacillus thermoleovorans LW-4-11 telah dimurnikan menggunakan amonium sulfat 70% diikuti dengan perlakuan panas (suhu 70°C selama 1 jam). Kemurnian enzim yangdipisahkan dari medium fermentasi meningkat 4.28 kali dengan aktivitas spesifik sekitar 43.7 mUImg. Enzim memiliki suhu dan pH optimum masing-masing 80°C dan 6.0 dalam substrat 0-hydroxyethylated chitin (glycol chitin). Kitindeasetilase ini relatif tahan panas dengan waktu-paruh sekitar 30 menit pada suhu 80 "C. Enzim dihambat oleh ion Li, ZnZ+, MnZ+, Co2 and Ni pada konsentrasi 1 mM, tetapi diaktifkan oleh EDTA 1 mM.Kata kunci: purifikasi parsial, karakterisasi, kitin deasetilase, B. thermoleovorans LW-4-11
Genetic Variation of Agathis loranthifolia Salisb. in West Jawa Assessed by RAPD Yunanto, Tedi; Djamhuri, Edje; Siregar, Iskandar Z.; Ulfah, Mariyana
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i1.3125

Abstract

ABSTRAKVariasi Genetika Agathis loranthifolia Salisb Jawa Barat Menggunakan Analisis RAPD.Agathis loranthifolia Salisb merupakan salah satu tumbuhan hutan yang menghasilkan hasilhutan bukan berupa kayu yaitu berupa getah. Untuk mengetahui derajad variasi genetika darijenis ini maka dicoba dianalisis menggunakan RAPD. Sampel daun tumbuhan ini di perolehdari Perum Perhutani Cianjur dan Garut. Variasi genetika populasi tumbuhan ini dari Cianjursebesar He = 0.1952 atau lebih tinggi dari populasi asal Garut (He = 0.1125). Namun berdasarkanproduksi copalnya menunjukkan bahwa variasi genetika pohon Lanang paling tinggi He =0.2105Kata kunci: RAPD, kopal, Agathis loranthifolia
Pengaruh Radiasi Sinar Gamma dan Asam Fusarat untuk Meningkatkan Ketahanan Abaka (Musa textilis Nee) terhadap Fasariurn oxysporum Damayanti, Fitri; Suharsono, Suharsono; Mariska, Ika
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.082 KB) | DOI: 10.14203/jbi.v3i4.3285

Abstract

ABSTRACTEffect of gamma radiation and fusaric acid for resistance to wild Fusarium disease onabaca plant (Mum textillis Nee). The problem in abaca production is wilt disease infectioncaused by Fusarium oxysporzim. The resistant variety against the pathogen has not beenavailable yet. The disease resistance character of the species might be improved throughsomaclonal variation and in vitro selection. Different pure toxin of fusaric acid concentration(0, 15,30,45,60, and 75 mg/l) was used as component selection to get new hope numbers ofresistant abaca to wilt Fusarium disease through in vitro selection. Concentration of 45 mgllfusaric acid is lethal for abaca, so we used this concentration as dose of selection to selectshoots from irradiated calli. Gamma irradiation was used as mutagent to increase somaclonalvariation on abaca. Six levels of gamma-ray radiation (0, 0.5, 1, 2, and 3 Krad) were appliedto embriogenic calli. Increasing dose of radiation decreased the viability of calli. LDso wasfound between 1-1.5 Krad of radiation dose. In vitro selection was carried out in two stages.The concentration of selection of hsaric acid in stage I1 was increased one level to theconcentration in stage 1. Stage I selection of shoots from irradiated calli on mediumcontaining pure toxin 45 mg/l fusaric acid, showed that the survival capacity decreasing withthe increasing doses of gamma irradiation. In stage 11, shoots from irradiated calli (at 0.5 and1 Krad) could survive on medium containing 60 mg/l fusaric acid. In medium selectioncontaining 50% filtrate F. oxysporum, fusaric acid resistant shoots were also filtrate resistant.There was a correlation between in vitro fusaric acid and filtrate of F. oxysporum resistantplant and conidia suspension of F. oxysporum resistkt plant in the greenhouse.Keywords: Gamma radiation, in vitro selection, fusaric acid, Musa textilis, Fusariumoxysporum
Emisi Gas Dinitrogen Oksida dari Tanah Sawah Tadah Hujan yang diberi Jerami Padi dan Bahan Penghambat Nitrifikasi Wihardjaka, A.
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.286 KB) | DOI: 10.14203/jbi.v6i2.3160

Abstract

ABSTRACTNitrous Oxide Emission from Rainfed Lowland Rice Soils through Applications of Rice Strawand Nitrification Inhibitor Materials. Alternate wet-dry of soil condition under rainfed lowlandsystem influence on source and sink dynamics of green house gases. Lowland rice soil is oneof antropogenic sources of nitrous oxide (N2O) emission produced by microbiologicalnitrification-denitrification mediated processes. Attempt to increase soil productivity in lowlandrice system by organic amendment is predicted to stimulate nitrous oxide production. Theincrease of N2O production in lowland rice could be suppressed by using nitrification inhibitormaterials. A field experiment was conducted in rainfed lowland rice during 2009 dry season. Theobjective was to study interaction of rice straw application and nitrification inhibitor materialson nitrous oxide emission from rainfed lowland rice. Experiment was arranged using factorialrandomizes block design with three replicates and treatment of rice straw application (withoutrice straw, fresh straw, composting straw) and inhibitor nitrification materials (without inhibitornitrification, neemcake, carbofuran). Interaction of rice straw and nitrification inhibitor materialsdecreased significantly N2O emission from lowland rice soil. Nitrous oxide emission in plotwithout rice straw was higher than in plot treated with neither fresh rice straw nor compostingstraw. Application neemcake combined with composting straw emitted lowest nitrous oxidewith flux of 72 g N2O ha-1 season-1, whereas the highest N2O emission was found in plot withoutnitrification inhibitor materials and rice straw with flux of 454 g N2O ha-1 season-1. Comparedwith treatment of without nitrification inhibitor, application of neemcake and carbofuran couldsuppress nitrous oxide emission of 48.6 and 41.3 %, respectively.Key words : nitrous oxide emission, rainfed lowland, rice straw, nitrification inhibitors
Phenetic Analysis of Gossypium Species (Malvaceae) from Indonesia Juswara, Lina Susanti
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i1.145

Abstract

This research was aimed to revise the species of Gossypium in Indonesia with statistical analysis of morphologicalcharacteristics. Three species of Gossypium were recognized based on phenetic analyses (cluster analysis-UPGMAand ordination-Principle Component Analysis) using 20 morphological characters and 18 samples of Gossypiumarboreum, G. barbadense, and G. hirsutum. Based on these analyses, two varieties were differentiated under G. arboreum(var. arboreum and var. acuminatum). The data set used for the analysis was robust in separating the samplesused in the analysis into discrete groups and cluster analysis-UPGMA and ordination-PCA were powerful tools forthe allocation of those samples into identifiable groups.Keywords: Phenetic analysis, Gossypium, Principal Component Analysis, UPGMA
Kemampuan Primer General CK4/CK2 Virus Infectious Bronchitis (IBV) untuk Mengaplifikasi Genom IBV Isolat Lapang Indonesia Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
The Dynamics of Species Composition Stand Structure and Above Ground Biomass of Undisturbed Forest in East Kalimantan Krisnawati, Haruni; Wahjono, Djoko; Imanuddin, Rinaldi
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.378 KB) | DOI: 10.14203/jbi.v8i1.3062

Abstract

Dinamika Komposisi Spesies Struktur Pohon dan Biomasa di Hutan Terganggu di Kalimantan Timur. Kajian dinamika komposisi spesies dan biomasanya dilakukan selama 4,3 tahun (Desember 2004 - April 2009) di hutan terganggu Kalimantan Timur. Untuk melakukan kajian di gunakan 6 plot permanen (100x100 m2), yang berada di hutan penelitian Samboja. Semua pohon berdiameter > 10 cm dbh (diukur pada posisi 1,3 m diatas tanah). Pada Desember 2004,tercatat 2143 pohon tersebar di plot tersebut terdiri dari 39 famili, 82 genus dan 111 spesies. Pada pengamatan April 2009 tercatat 2466 pohon terdiri dari 40 famili, 86 genus dan 123 jenis.Sebagian besar spesies yang mendominasi adalah Garcinia nervosa dan Trigonostemon laevigatus tetapi setelah pengamatan pada tahun 2009, keduanya tidak lagi mendominasi. Selama kurun 4,3 tahun terjadi penambahan kepadatan 15,1%, basal area 12,9% dan biomasa 11,6%. Penambahan kepadatan tercatat meningkat dari 357 menjadi 411 pohon/ha. Basal area meningkat mulai dari 20,09 menjadi 22,67 m2 ha-1, sedangkan biomasa meningkat dari 286,3 menjadi 319,4 ton ha-1.Kata kunci: Komposisi, spesies, biomas, hutan terganggu
The Role of Coprophagous Beetles on Dung Decomposition and Enhancement OF Soil Fertility: Effect Of Body Size, Species Diversity and Biomass Shahabuddin, Shahabuddin; Manuwoto, Sjafrida; Hidayat, Purnama; Noerdjito, Woro A.; Schulze, Christian H.
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i2.3192

Abstract

ABSTRAKPeran Kumbang Koprofagus pada Dekomposisi Kotoran Hewan dan Kesuburan Tanah:Pengaruh Ukuran Tubuh, Keragaman Species dan Biomasa. Penelitian ini bertujuan untukmenganalisis pengaruh keragaman species, ukuran dan biomassa kumbang koprofagus dalammerombak kotoran hewan dan meningkatkan kesuburan tanah. Percobaan dilakukanmenggunakan Rancangan Acak Lengkap dengan perlakuan jumlah dan panjang tubuh spesieskumbang koprofagus. Peubah tergantung yang diamati adalah persentase kotoran yangterdekomposisi dan kadar bahan organik serta N,P,K total tanah sebagai indikator kesuburantanah. Hasil penelitian menunjukkan bahwa persentase kotoran hewan yang terdekomposisilebih dipengaruhi oleh ukuran dan biomassa kumbang yang terlibat dibandingkan denganjumlah spesies. Persentase kotoran yang terdekomposisi berkorelasi positif dengan ukurankumbang koprofagus. Kadar N,P,K total tanah meningkat mengikuti jumlah kotoran hewanyang terdekomposisi yang mengindikasikan bahwa aktifitas perombakan kotoran hewan olehkumbang koprofagus berpengaruh positif terhadap kesuburan tanah.Kata kunci: Kumbang koprofagus, komposisi spesies, dekomposisi, kesuburan tanah
Kepadatan Ikan Napoleon (Cheilinus undulatus) di Perairan Sinjai dan Bone-Sulawesi Selatan Syam, Amran Ronny; Mujiyanto, Mujiyanto
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.972 KB) | DOI: 10.14203/jbi.v10i1.327

Abstract

The exploitation of Napoleon fishery rates (Cheilinus undulatus) in the past year has been higher. Some areas inIndonesia are still doing the fishing business. Since the napoleon fish was registered in the IUCN red list &Appendix II of CITES, the export quota of 3,600 fishes per year was applied up to the year 2011. In order tosupport the evaluation size in the water bodies around Sinjai and Bone of the determination of napoleon fishprotection status, this study aims to determine fish population South Sulawesi. The method used was SnorkelingVisual Census (SVC). The results obtained indicate napoleon fish abundance is low (0 to 4 individuals/ha). Highestabundance of napoleon fish was found in Lapoipoi & Batanglampe, however none in three locations, i.e., Larearea,Pasiloange and Malambere. It is suggested that protection to species & its habitat is done in four locations wherethe napoleon fish were found, especially in Lapoipoi & Batanglampe.Keyword: napoleon fish, density, Sinjai, Bone, South Sulawesi

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