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JURNAL BIOLOGI INDONESIA

jurnal_biologi_indonesia
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08544425 EISSN : 2338834X DOI : -

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology

Arjuna Subject : -

Articles 847 Documents

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Bioakumulasi Kadmium Pada Kerang Hijau (Perna viridis) Dengan Aplikasi Perunut Radioaktif Siregar, Yusni Ikhwan
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTBioacumulation of Cadmium on Green Mussel (Perna viridis) Using Radiotracer. A laboratoryexperiment on the accumulation of Cadmium (Cd) by green mussel (Perna viridis) has beenconducted. Radiotracer 109Cd was applied in the study. The research aimed at evaluating theeffect of salinity, temperature and size on uptake of radiotracer 109Cd by green mussel (Pernaviridis) from dissolved phase. It was found that both salinity and temperature had significanteffect ( P< 0.001) on accumulation rate of 109Cd. The highest concentration factor of Cd (31,23-54,09) was appeared in water salinity of 29% and of water temperature 30oC. At the steady statecondition green mussel accumulated 72,21-107,80 Bq/gr Cd. It revealed that the small Pernaviridis (5.2 cm in length) accumulated 109Cd about 107,80 Bq/gr, whereas the bigger size ofPerna viridis (6.6 cm in length) had an uptake of about 72,21 Bq/gr.Key words : Bioaccumulation, radiotracer Cd, Perna viridisKata kunci : Bioakumulasi, Perunut radioaktif, Cd, Perna viridis

Identifikasi Keragaman Khamir yang Diisolasi dari Tanah Kebun Biologi Wamena Kabupaten Jayawijaya, Propinsi Papua Kanti, Atit; Latupapua, H.J.D.
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThe Identification of Yeasts Which Were Isolated from Soils in the Wamena Biological Garden, Jayawijaya Residence, Papua Province. The present study deals with the isolation and identification of yeasts isolated from soil samples in The Wamena Biological Garden, Papua. Microbial population in 11 samples were estimated by plate count method. The yeasts population were ranged from 30.0 x102 until 16.5 x106 cells/g soil. Fifteen isolates of yeasts were taxonomically studied. On the basis of morphological and physiological characteristics these isolates were belonging to two group namely ascomycetous-imperfect yeasts and basidiomycetous yeasts. Futher, they were separated into three group. Out of 15 isolates, eleven isolates were included in group I and identified into genus level as Cryptococcus, three in group II as Candida and one isolate in group III identified into species level as Cryptococcus albidus. Type of sample sources appeared has no effect on yeasts diversity as shown by similar yeast isolate was observed isolated from different sources.Key words : Ascomycetous-imperfect yeasts, basidiomycetous yeasts, The Wamena Biological Garden, Papua.

Perbanyakan Amorphophallus titanum Becc (Araceae) dengan Teknologi In Vitro ,, Witjaksono; Nugraheni, Katarina Utami; Hoesen, Djadja SH; ,, Irawati
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3056

Propagation of Amorphophallus titanum Becc (Araceae) via In Vitro Technology. Amorphophallus titanum is a unique plant with gigantic inflorescence and radiating corpselike odorl and therefore has become a botanical flagship and attract visitors. Its existence innature may be endangered due to habitat conversion that reduce population size required for cross pollination to occur. Propagation via non-conventional technique is much needed for the resulting planting material to be exchanged among botanical garden or even traders. Mass propagation using in vitro technology has been successful. An improvement of the propagation method has been developed by incorporating high level of cyitokinin BA of 1-2 mg/l in the medium for shoot proliferation and lowering it to 0-0,1 mg/l for shoot elongation. Shoot elongation is effectively influenced by age and the phase of medium whereas the liquid phase induced the enlargement of the shoots significantly without even promoting any hyperhidricity.The plantlets produced survive acclimatization.Key words: Amorphophallus titanum, mass propagation, in vitro, mediurm cair, BA.

Efek Penghambatan Terhadap Pertumbuhan Tumor Paru dan Uji Ketoksikan Akut Ekstrak Kapsul Chang Sheuw Tian Ran Ling Yao Pada Mencit (Mus musculus) dan Tikus (Ratus tanezumi) Fudholi, Ahmad; Meiyanto, Edy; Donatus, Imono Argo; Nurrochmad, Arief; Hakim, Arief Rahman; Murwanti, Retno
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Inhibitory Phases Effect of The Lung Cancer And Acute Toxicity of Chang Sheuw Tian RanLing Yao Capsule Extracts in House mice (Mus musculus) and Rat (Ratus tanezumi). Effortsto find anticancer agents have been developed nowadays, some of them are focused in traditionalherbs. One of the available products in the market that claims effective to cure cancer isthe Chang Sheuw Tian Ran Ling Yao, PT. Daun Teratai extract containing CAPSULE (CSTRLYextract). The aim of this study is to examine of confession of some people which are usingthe useful of medicine CSTRLY extract capsule through inhibitor laboratory effect of theCSTRLY extract in the initiation and post initiation phases of the lung cancer in mice and ratsthat had been induced by eather Benzo[?]pyrene (BP) or Dimetilbenz[?]antrazene (DMBA)and to clarify the potency of acute toxicity and specific toxic manifestations of thephytopharmaca.The results showed that the CSTRLY extract can reduce the cancer incidence caused bycarcinogen, BP and DMBA. Moreover, the extract can also inhibit the cancer growth in themice and rats, especially in the early post-initiation phase. Further, the histopathologicalevaluation showed that up to the highest dose level that technically could be administrated tothe animals (12500 mg/kg bw), no animal death was occurred. Furthermore, the ADG values formale and female rats indicated no significant different (P > 0.05) that relative to the controlgroup. No animals were shows physical symptom as a toxic manifestation. Itâs indicated thatthe phytopharmaca no influenced to somatomotor and nervous system. Within the dose rangeadministrations, no detectable morphological toxic effects or histophatological changes of theliver, spleen, heart, and lungs were observed. the acute toxicity value of Chang Sheuw TianRan Ling Yao Capsule was very low (or minimal almost non-toxic with LD50 > 12500 mg/kg bw)and the spectrum of toxic effects of the phytopharmaca were considered negligible.Key words: Ekstract, CSTRLY, mice and rat, BP, DMBA, carsinogenesis, lung cancer

Pengaruh Suhu, pH, Enzim dan Surfaktan terhadap Plantarisin F Rekombinan Enkapsulasi sebagai Antibakteri Staphylococcus aureus dan Salmonella typhi Mustopa, Apon Zaenal; Hasim, Hasim; Amelia, Suci
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3664

ABSTRACTStaphylococcus aureus causes diarrhea, which is one of the main cause of infant mortality, where asSalmonella typhi causes typhoid fever with the incidence rate of 180/100,000/year. Plantarisin F is anantimicrobial peptide that can inhibit the growth of S. aureus and S. typhi. The aim of this study is to determinethe effect of temperature, pH, enzymes, and surfactants of encapsulated F recombinant plantarisin. Plantarisin F(1.61%) encapsulated with maltodextrin (5.36%) and skim milk (2.68%) using the spray dry with inlettemperature 150ÂºC produced particles that are generally spherical with a rough texture, range in size, yield25.03%, and had good antibacterial activity against S. aureus and S. typhi. The antibakterial activity plantarisinF encapsulated is not affected by the treatment temperature (40ÂºC-100ÂºC), pH (2-12), enzyme (proteinase-K,catalase, lysozyme, pepsin, trypsin), dan surfaktan (SDS, urea, triton X-100, PMSF, EDTA) treatment.Keywords: Antibacterial, Encapsulation, Plantarisin F, Salmonella typhi, Staphylococcus aureus

Identifikasi Protein dan Seleksi Isolat Trypanosoma evansi Bersifat Imunogenik untuk Kandidat Pengembangan Imunoasai Subekti, Didik T.; Yuniarto, Ichwan
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTSurra is a parasitic disease caused by T. evansi and causing high economic losses in Indonesia. Some isolates have been isolated from several areas experiencing outbreaks of Surra in Indonesia. The isolates have been reported to have a diversity of protein profiles based on SDS PAGE (sodium dodecyl sulphate polyacrilamide gel electrophoresis). The research aims to identify immunogenic protein from each isolate and make the selection of T. evansi isolates which is potential as a source of antigens for immunoassay development. Each isolates were obtained to be purified from blood and then the protein was isolated. The proteins were run onto polyacrylamide gel electrophoresis and visualized by Coomassie blue. Another electrophoresis results were transferred onto a nitrocellulose membrane for immunoblotting. The results showed that the immunogenic proteins that consistently detected among nine T. evansi isolates of Indonesia are 100, 90, 85, 76-80, 70, 65, 55, 49-52, 44-46, 40, 34-36, 31 -33 kDa. Among the nine T. evansi isolates, N372 isolate was selected as a candidate for immunoasay development, especially ELISA. Immunogenic proteins were specifically found on the N372 isolate are 85, 70, 65, 49-52, 44-46, 34-36, 31-33, 24-28, 15-20 kDa.Keywords : Trypanosoma evansi, immunoblotting, protein profiles, immunogenic protein

Respon Tanaman Jewawut [Setaria italica (L.) P. Beauv.] terhadap Kondisi Cahaya Rendah Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTMillet [Setaria italica (L.) P. Beauv.] is one of the potential cereal crops to be developed in supporting food diversification program. The purpose of this study was to investigate the response of millet to low light (shade) condition. The experiment was conducted in the field with nested design that consisted of two factors that are, 1) Accession from Buru Island which are yellow seed and red seed; and 2) Shading nets that consisted of 0%, 40%, and 70% shade. Shade factor was designed as main plot, while accession factor was designed as sub plot. Growth (growing percentage, plant height, leaf number, leaf area, and plant dry weight), yield (panicle length and panicle weight), photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid), and stomata density of the millet were observed. The result showed that the growth and yield of both accession were decreased by the increase of shading. However, yellow seed millet tended to be more tolerant than red seed millet with optimizing photosynthetic capacity through enhancing leaf area, leaf longevity, and enhancing antenna pigments such as chlorophyll b and carotenoid.Keywords: Growth, millet, photosynthetic pigments, shading, yield

Benthic Molluscs Communities in the Intertidal Coast of Tanimbar Islands, West Southeast Mollucas Heryanto, Heryanto
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThis paper presents the results of a survey on intertidal molluscs in 3 habitats (Seagrass bed, coral reef, and sandflat) in Tanimbar Islands of West South-east Moluccas, Indonesia. Six relatively large and two small islands were attained to perform 125 plots of 34 stations. Molluscsâ diversity in each habitat were calculated using Shannon-Wiener formulation whereas Mann-Whitney formulation for comparing between habitats. Multivariate analyses for clustering of PAST 2.17 was utilized to further the analyses. A number of 101 species gastropods (26 families) and 51 species of bivalves (19 families) were recorded. Seagrass bed was inhabited by 100 species, followed by coral reef by 85 and sandflat by 44 species. Shannon-Wiener indexes for molluscsâ diversity range in seagrass was 0.39 to 1.29, in coral was 0.27 to 1.14 and in sandflat was 0.52 to 0.99. Clustering analyses for stations revealed that three stations in seagrass were the most dissimilar, followed by one station in coral due to habitat. Clustering analyses for species found four dissimilar groups of molluscs, whereas a group has many consimilar members.Keywords: Seagrass, coral, sandflat, diversity, clustering

Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(?)Piren Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

ABSTRACTCancer is a disease marked by the existence of abnormal growth of cell continuously and uncontroll in an organism body. Benzo(?)pyrene is one of the substances that can cause a cancer. The methanolic extract of Sargassum sp. and Gracillaria sp. has anticancer and antioxidant activity. Other compounds with antioxidant activity is taurine. The aim of this research is to examine the effect from methanolic extract of Sargassum sp. and Gracillaria sp .and also taurine on hepar histopatology of male mice (Mus musculus) induced by benzo(a)pyrene. This research used Completely Randomized Design. 30 male mice were divided into 6 groups of treatments, those were: K1 = (K-), K2= induced by benzo(?)pyrene for 10 days, K3= after induced by benzo(?)pyrene, were given the Sargassum sp. orally with a dose 8mg/mice during 15 days, K4=after induced by benzo(a)pyrene, were given the combination of Sargassum sp. (8mg/mice) and taurine(15,6mg/mice) orally during 15 days, K5= after induced by benzo (?)pyrene, were given the Gracillaria sp.orally with a dose 8mg/mice during 15 days, and K6=after induced by benzo(a)pyrene, were given the combination of Gracillaria sp. (8mg/mice) and taurine (15,6mg/mice) orally during 15 days. Data were analyzed using ANOVA (? = 5%). The result shows that the combination of Sargassum sp. and Gracillaria sp. and taurine can increase the number of erythrocyte , decrease the number of leucocyte and protect the damage of hepar histopatology of male mice (Mus musculus) induced by benzo (?)pyrene. Combination of extract Gracillaria sp. and taurine is the most effective to protect the damage hepar histopatology of male mice(Mus musculus) induced by benzo(? )pyrene.Keywords: male mice,Sargassum sp., Gracillaria sp., taurine, benzo(a)pyrene, hepar

Efektivitas Proses Fitoremediasi Air Irigasi Tercemar Bahan Organik melalui Sistem Batch Culture menggunakan Hidromakrofita Lokal Retnaningdyah, Catur; Arisoesilaningsih, Endang
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTIrrigation water in Indonesia generally has polluted by organic matter due to human activities. The aim of study was to develop a package of phytoremediation technology that can effectively reduce organic pollutants in the irrigation water in order to ensure good quality of water irrigation to support organic farming. The study was conducted in greenhouses by planting some local emergent, submerged, floating-leaf hydromacrophytes and polyculture species using a batch culture system on a 30 L bucket with sand and gravel as a substrate. The study used a completely randomized design with the treatment were five types of hydromacrophytes (emergent such as Scirpus sp., Acorus calamus, Marsilea crenata; floating leaf macrophytes included Ipomoea aquatica, Azolla sp.; submerged such as Valisneria sp., Hydrilla verticilata; polyculture of the three hydromacrophytes, and controls without plant) which repeated three times at the same time. The effectiveness of the phytoremediation model for each treatment is determined by its ability to improve water quality as reflected by several physicochemical parameters of pH, DO, conductivity, turbidity, temperature, nitrate, orthophosphate, bicarbonate, BOD, TSS, TDS and TOM six days after incubation. Research results showed that batch culture for six days significantly reduced the organic matter content of irrigation water reflected from decreasing value of BOD, TOM, TSS, turbidity, nitrate, orthophosphate and bicarbonate levels. The six-day phytoremediation process in all treatments has not been able to increase the dissolved oxygen content in the water and has not been able to significantly reduce the conductivity and TDS. Hydromacrophytes submerged and polyculture were more effectively perform phytoremediation process compared with others.Keywords: Phytoremediation, local hydromacrophytes of emergent, submerged, and floating leaves, water pollution

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