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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 63   64   65   66   67 
BIODEGRADASI PHENANTRENE OLEH MIKROBA LAUT M5 (ALCANIVORAX BORKUMENSIS) YANG DIISOLASI DARI TELUK JAKARTA Supriyati, Dyah
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3178

Abstract

ABSTRACTBiodiversity of Phenanthrene by Alcanivorak borkumensis M5 Isolated from Teluk Jakarta.Phenantrene is one of the most persistent organic substances in environment. AlcanivoraxBorkumensis M5 was isolated from sea water, and able to degrade phenantrene after 5 hours.About 75 % of phenantrene was degraded after 12 hours. This isolate grew optimum at 300C,pH 7.8 with a doubling time of 14.5 hours and specific growth rate of 0.0476/hour.Polyhydroxybutirate (PHB) was produced during culture growth, but the synthesis wasinversely correlated with the cell growth. The relation between PHB synthesis and phenantrenedegradation is due required further investigation.Keywords: Phenanthrene, degradation ,bacteriaKata kunci: Phenantren, degradasi, bakteri
RESPON ENZIM ANTIOKSIDATIF SONCHUS OLERACEUS TERHADAP CEKAMAN KROM PADA MEDIA TANAM BERBEDA Sucahyo, Sucahyo; Kasmiyati, Sri
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3661

Abstract

ABSTRACTSonchus oleraceus is a weed that is capable of living in chromium contaminated environments, so their physiological, biochemical and molecular responses can be used as biomarkers of chromium stress. Changes in the activity of antioxidative enzymes can be used as a biomarker of biochemical against heavy metal stress.The objective of this research was to know the response of antioxidative enzymes activity in S. oleraceus subjected to Cr stress on different planting media. The research was done in 2 factors i.e. planting media types and Cr compound types (Cr3+and Cr6+). Parameters measured were antioxidative enzymes activity i.e catalase (CAT), ascorbate peroxidase (APX), superoxidase dismutase (SOD), and glutathion reductase (GR), dissolved protein content in leaves, and content of Cr6 + and Cr total in roots and shoots.The data were analyzed with a two way ANOVA using SAS 9.1 32-bit for windows. The results indicated that activity of antioxidative enzymes (CAT, APX, SOD and GR) of S. oleraceus leaves tend to increase under Cr stress condition. The Cr6+ treatment of 10 mg/L significantly increased the antioxidative enzyme activity of CAT, APX, SOD and GR in S. oleraceus leaves. The antioxidative enzyme activity (APX, SOD and GR) S. oleraceus grown on soil medium containing sludge was higher than that grown on soil media and on the mixture of sludge: soil (1: 1). Under Cr stress conditions, the roots and shoots of S. oleraceus grown on sterile sand media, soil and the mixture of sludge:soil (1:1) accumulate Cr6+ and Cr total. The accumulation of Cr6+ was found the highest in shoots, meanwhile accumulation of Cr total was found the highest in the roots. The Cr treatments and planting media also significantly influenced the soluble protein content in S. oleraceus leaves.Key words: antioxidant, oxidative stress, chromium, weed, soluble protein
OPTIMASI ENZIM ?-AMILASE DARI BACILLUS AMYLOLIQUEFACIENS O1 YANG DIINDUKSI SUBSTRAT DEDAK PADI DAN KARBOKSIMETILSELULOSA Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
PENAPISAN MIKROBA LAUT PEROMBAK SENYAWA NITRIL DAN PROTEIN YANG DIISOLASI DARI SPONS DI PERAIRAN TERNATE Riffiani, Rini; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3143

Abstract

ABSTRACTScreening of Nitrile and Protein-Degrading Marine Bacteria Isolated from Sponge in TernateSea Water. Thirty three marine bacteria have been isolated from marine sponge in Ternate byenrichment culture. Screening bacteria-degrading nitrile was done by microtitter plate methodbased on growth ability tested by Iodonitrotetazolium chloride. Product of nitrile degradationwas determined by Gas Chromatography (GC) and the potential bacteria-degrading proteinwas also screened by using selected media which contained casein. The results showed thattwenty one isolates were able to show the clearing zone in selected media. Five isolatescapable of utilizing acetamide as the sole source of carbon and nitrogen. Acetate and ammoniaproduced for hydrolysis acetonitrile by using resting cell of Lysobacter sp.Key words: Nitrile, bacterium, sponge, Ternate
INTRODUKSI KONSTRUK GEN CSNITR1-L DENGAN PROMOTOR UBIQUITIN MELALUI AGROBACTERIUM TUMEFACIENS DAN DETEKSI MOLEKULERNYA PADA PADI KULTIVAR NIPPONBARE Enngarini, Wening; Polosoro, Aqwin; Sustiprijatno, Sustiprijatno; Trijatmiko, Kurniawan Rudi
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3400

Abstract

ABSTRACTNitrogen based fertilizers such as urea and NPK are primary needs for rice farmers. To get significant improvement of crop yield, the more quantity of fertilizers are applied. It make negative impact for surrounding environment. Based on that, the efforts should be done to suppress the demand of fertilizers such as by developing Nitrogen Use Efficiency crops. CsNitr1-L is one of gene that related to Nitrogen Use Efficiency trait in plant. The objectives of this research are to develop the construction of CsNitr1-L gene candidate in pCAMBIA1300-Ubi1 promoter and to obtain the transformants of rice cultivar Nipponbare which contain the construction of CsNitr1-L gene candidate. The construction of pCAMBIA1300::Ubi1::CsNitr1-L has successfully assembled and was transformed to immature embryo of rice cultivar Nipponbare using Agrobacterium tumefaciens strain LBA4404. It was obtained 146 lines of T0 Nipponbare. PCR analysis of T0 Nipponbare lines showed that 66 of them was identified as positive T0 lines contained hptII and CsNitr1-L genes. Transformation efficiency obtained was 11,9%. The result of analysis copy number using Southern Hybridization in positive PCR of T0 lines randomly indicated that 4 lines have a single copy of transgene. Based on these results, it can be concluded that CsNitr1-L gene construct was successfully introduced into the genome of the rice plant cultivar Nipponbare and the positive PCR of T0 lines containing the gene of hptII and CsNitr1-L, also a single copy of the transgene was obtained.Keywords: Nitrogen use efficiency trait, gene construction, rice kultivar Nipponbare
MORPHOLOGICAL IDENTIFICATION AND DIVERSITY ANALYSIS OF FOSSIL DIATOMS FROM DIATOMITE SANGIRAN CENTRAL JAVA INDONESIA Khustina, Yenny Chusna; Solihin, Dedy Duryadi; Pratiwi, Niken TM
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2105

Abstract

Diatomite Sangiran is diatom fossil rich sediment. The aim of this research was to study the diversity of fossildiatoms from Sangiran, Indonesia based on morphological characteristics. Samples were taken from lower, middle,and upper sediment layers based on their different physical features with three replications each. Diatomiteextraction was performed following modification of Setty (1966) and frustule counting was accomplished by censusmethod. There were total 50 species found in diatomite layers. The diversity analysis showed that highest speciesrichness (21-22), diversity (1.35-1.47), and evenness index (0.44-0.48) were belong to the lower layer. The highestfrustule abundance (9.66x107-1.43x108 frustules/gram) and dominance index (0.67-0.72) were belong to themiddle layer. On the other hand, highest centrales:pennales ratios (0.73-1.11) were belong to the upper layer. Thesediment layers signified an obvious indication of gradual changes from marine to freshwater environment.Dendrogram analysis using MINITAB.v.15.1.2 software denoted similarity between lower sediment layer and theyounger layers was 72.12%; while similarity between middle and upper layers 92.63%.Keywords: diatom, diatomite, diversity, morphology, Sangiran
NOTES ON THE MASS OCCURRENCE OF THE CILIATE MESODINIUM RUBRUM (NON-TOXIC RED TIDE) IN MALACCA RIVER, MALAYSIA Pau, Suriyanti Su Nyun; Nasir, Dzulhelmi Muhammad; Usup, Gires
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3106

Abstract

SHORT COMMUNICATION
KELIMPAHAN RELATIF BURUNG-BURUNG DI HUTAN PEPNUNGAN TILU GEDER, GARUT SELATAN, JAWA BARAT Widodo, Wahyu
JURNAL BIOLOGI INDONESIA Vol 4, No 2 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i2.3270

Abstract

TULISAN PENDEKKelimpahan Relatif Burung-Burung di Hutan Pepnungan Tilu Geder,Garut Selatan, Jawa Barat
PALEM DI TAMAN NASIONAL BUKIT BAKA-BUKIT RAYA, KALIMANTAN BARAT Rustiami, Himmah
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.172

Abstract

Field work of palm diversity in Bukit Baka-Bukit Raya National Park had been conducted and revealed 17 species ofpalms, namely Caryota, Iguanura, Licuala, Pinanga, as well as 4 Calamus spp., 5 Daemonorops spp., and 3 Korthalsiaspp. Seven species out of those seventeen species were recorded as endemic in Kalimantan, namely Caryota no,Iguanura macrostachya, Licuala borneensis, Pinanga tomentella, Calamus pogonacanthus, Daemonorops microstachys danDaemonorops sp. 1. Identification key, species description, synonym and other information related to the speciesreported in this paper.Keywords: Palm diversity, Bukit Baka-Bukit Raya National Park, endemic, Kalimantan
INDUKSI POLIPLOIDI TANAMAN KANGKUNG (IPOMOEA AQUATICA FORSSK.) KULTIVAR SALINA IN VITRO DENGAN ORYZALIN Rahmi, Putri; Witjaksono, Witjaksono; ratnadewi, diah
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3760

Abstract

ABSTRACTWater spinach is a vegetable plant consumed by people all over the world. Its small morphology requires improvement to increase the size and productivity. An alternative way to do that is by increasing the ploidy of its chromosome. This paper describes in vitro induction of polyploidy of water spinach. Inoculum of in vitro seedling, in vitro shoots and nodal stem segments were immersed in MS solution containing oryzalin at concentrations of 0.00, 1.25, 2.50, 3.75 and 5.00 µM with immersion duration of 4, 8 and 24 hours. Oryzalin treatments reduced the growth variables of the in vitro shoots of all inocula tested, compared to control of no oryzalin treatment.  On immersion of 24 hours and high concentration of 5.00 µM, many inoculum failed to grow to the third passage of subculture. The best growth of in vitro shoot to the third passages occurred in the treatment combination of oryzalin 1.25 µM-8 h immersion and 2.50 µM-4 h immersion for seedling inoculum, treatment combination of 3.75 µM-4 h immersion for shoot tip inoculum and 1.25 µM-4 h immersion for nodal stem segment, compared to other treatments. Analysis of flow cytometry on 41 leaf samples from oryzalin treatment derived shoots showed 14.63% tetraploid, 36.59% mixoploid and 48.78% diploid. The efficiency of tetraploid formation reached  60%, provided only by the treatment of oryzalin 1.25 µM-8 h on seedling segments inoculum. Tetraploid shoots need to be proliferated, acclimatized, grown into planting materials and planted for agronomical analysis to provide evidence whether or not tetraploid water spinach is viable for commercial cultivation. 

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