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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 18 Documents
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Search results for , issue "Vol 21 No 1 (2020)" : 18 Documents clear
Analisis Hemagglutinin Virus Flu Burung (Avian Influenza) Subtipe H5N1 Asal Pasar Unggas Hidup di Jakarta Timur 2014 (HEMAGGLUNIN ANALYSES OF AVIAN INFLUENZA VIRUSES SUBTYPE H5N1 ISOLATED FROM LIVE BIRD MARKETS IN EAST JAKARTA 2014) Risza Hartawan; Ni Luh Putu Indi Dharmayanti
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Hemagglutinin is a surface glycoprotein of the influenza virus encoded by segment 4, which has the main function to attach to the host cell receptor. This glycoprotein is one of the most important markers for determining viral pathogenicity and virulence. Furthermore, hemagglutinin also has experienced massive mutations since its epitopes exposed to the external environment resulting in various variants in the field. The main objective of this study was to characterize molecularly the avian influenza virus subtype H5N1 isolated from live bird markets in East Jakarta in 2014 using a bioinformatics approach. As a result, this study identified the circulation of the highly pathogenic virus from both clade 2.1.3 and 2.3.2 with several mutations in the hemagglutinin sequence. These viruses still displayed a high affinity for the avian receptor. Subsequently, the prediction of the 3D structural model of hemagglutinin demonstrated differences in the protein conformations, especially in the HA2 domain and cleavage site. These molecular predictions require further investigation studies, especially in vivo experiments with animal models.
Uji Kelarutan Kristal Kalsium Oksalat Urin Tikus Sprague Dawley setelah Pemberian Eliksir Ekstrak Etanol Daun Pelawan (SOLUBILITY TEST OF CALCIUM OXALATE CRYSTAL IN SPRAGUE DAWLEY RAT URINE AFTER GIVING ETHANOL EXTRACT OF PELAWAN LEAVES) Rahmawati Januar; Ietje Wientarsih; Sus Derthi Widhyari
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Traditional medicine is an herbal medicine used from generation to generation by people in Indonesian. Pelawan leaves is believed can dissolve crystals in the urine that can be the initial cause of kidney stone formation. This study aimed to evaluate the potential of elixir of the ethanol extract of pelawan leaves as antilithiasis in rat was given ethylene glycol and ammonium chloride as inducer. Thirty male rat Sprague dawley was divided in five different groups (n=6). The control group (K1), ethylene glycol (EG) and ammonium chloride (AC) as inducer (K2), inducer and Batugin 5,4 mg (K3); inducer and the elixir of the ethanol extract ofpelawan leaves (EEEDP) 100 mg/kg body weight (K4) and 200 mg/kg body weight (K5). The treatments were done in 28 days with urines observated at day 14 and day 28. The result showed that pelawan contain flavonoid, tannin, saponin and steroid. The result showed that treatment groups dissolved calcium oxalate crystals. Observated at day 14, K3 dissolved calcium oxalate crystals with lowest number, while at day 28, K4 dissolved calcium oxalate crystals with lowest number. A dose of 100 mg/kg body weight in the K4 group effectively dissolves the calcium oxalate crystals.
Hematological Profiles of Green Sea Turtle (Chelonia mydas) Rehabilitated at Turtle Conservation and Education Center, Serangan Island, Denpasar, Bali Leoni Arifah Agustina; Nyoman Sadra Dharmawan; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Threat of extinction results in the increasing sea turtle conservation efforts through scientific research in recent years. During rehabilitation process, the non-invasive blood analysis is considered to be highly valuable to determine sea turtle’s health. The objective of this study was to assess the hematological profiles of green sea turtles (Chelonia mydas) under rehabilitation at Turtle Conservation and Education Center (TCEC) Serangan Island, Denpasar, Bali. Three mililiters of peripheral blood from healthy green sea turtles were collected from cervical dorsal sinuses and kept in heparinized vacutainer tube before proceeded with hematological evaluation. PCV was determined by microhematocrit method. Diff count and thrombocyte percentage were determined using Giemsa-stained blood smear under the microscope. RBC and WBC total count were determined using hemocytometer. Hemoglobin (Hb) value was determined using Sahli method. Each hematological values were then analyzed using descriptive analysis. The correlation between body condition index (BCI) and hematological values were analyzed using Spearman’s rank correlation test. The mean values evaluated was 7.28 g/dL in Hb, 31.35% in PCV, 0.67 x 106/?L in total RBC count, 479.37 fl in MCV, 111.38 pg in MCH, 23.42 g/dL in MCHC, 4.01 x 103/?L in total WBC count, 1.20 x 103/?L in lymphocyte, 0.21 x 103/?L in monocyte, 0.10 x 103/?L in eosinophil, 2.48 x 103/?L in heterophil and 0.01 x 103/?L in basophil. No significant correlation was found between the BCI and hematological profiles evaluated.
Penambahan Water Additive Kimchi Dapat Mencegah Infeksi Alami Flu Burung (Avian Influenza) pada Ayam Pedaging (THE SUPLEMENTATION OF WATER ADDITIVE KIMCHI COULD PREVENT NATURAL INFECTION OF AVIAN INFLUENZA IN BROILER) I Putu Cahyadi Putra; Raden Wasito; Hastari Wuryastuty
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Subclinical infection of avian influenza (AI) in day old chick (DOC) has potential to interfere the success of vaccination programs. Therefore, an alternative ways to increase avian innate immunity in preventing the infection is necessary. The purpose of this study was to evaluate the effect of adding water additive (KimchiStock®) in preventing the occurrence of AI natural infection in DOC raising in commercial broiler farms. Fifty-five broiler DOCs, Cobb strains were used as experimental animals. Before the experiment begin, five DOCs were randomly selected, sacrificed and the lung were taken out for polymerase chain reaction (PCR) analysis on the matrix region. Fifty remaining DOCs were randomly divided into two: K1 (control) and K2 (treated) groups. K2 group were given 0.2% water additive (KimchiStock®) 5 days/ week for five weeks. Every week for five consecutive weeks, five chicken from each group were randomly selected for sera and lung collection. Sera samples were analyzed for antibody titer detection using haemagglutination inhibition technique. The results showed that there is no significant different in antibody titer between control and treated group. The majority of the antibody titer in this experiment were under 23. At week 4 the antibody titer of one chicken in treated group was 23 but no clinical sign was observed. Molecularly, there were no positive result detected from the lung of five DOCs at the beginning of the experiment and control group. While in the K2, positive result were detected on week 1 and 2 but without any clinical sign. Based on the research results could be concluded that adding 0.2% water additive KimchiStock® could prevent AI natural infection starting from week 3.
Gambaran Kelistrikan Jantung Anak Babi pada Kondisi Renjatan dengan Resusitasi Hipervolemik Menggunakan Cairan Kristaloid Natrium Chlorida 0,9% Gunanti Gunanti; Soesatyoratih Soesatyoratih; Antonius H Pudjiadi; Melpa Susanti Purba; Galih Satria Kusumanto
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aimed to determine the effect of resuscitation and hypervolemic conditions on shocked piglet’s heart electrical system. Animal models that were used in this study are 5 healthy castrated piglets. The piglet’s blood were withdrawn until the mean arterial pressure dropped about 20 % from the baseline condition to make a hypovolemic shock condition. When the piglets reached the hypovolemic shock state, fluid resuscitation using crystalloid (NaCl 0.9 %) as much as 40 ml/kg were given using a 50 ml syringe through the jugular vein. ECG examination were performed before surgery and when it reached the hypervolemic state after being resuscitated. The ECG results indicate no apparent change in cardiac performance due to the absence of ECG waveform changes from waveforms during normal conditions. In conclusion, crystalloid fluid resuscitation (NaCl 0.9 %) until hypervolemic condition does not interfere with cardiac performance.
Kadar Krioprotektan Gliserol dan Dimethylsulfoxide Terbaik pada Pengencer Astaxanthin Fosfat Kuning Telur Bebek Terhadap Kualitas Semen Beku Babi Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study to examine the addition of cryoprotectant glycerol and dimethylsulfoxide (DMSO) in astaxanthin phosphate duck egg yolk on the quality of frozen boar semen. Each of the cryoprotectants distinguished its concentration ie : 4%, 6%, 8%, 10%, and 12% for freezing of semen. Semen was collected from one Landrace boar then performed macroscopically and microscopically. Semen was divided into 10 tubes each diluted with the duck egg yolk astaxanthin phosphate extender diluent then stored at 20oC for 2 hours, centrifugation for 15 minutes (2000 rpm) then taken the pellet with 1 ml of supernatant. The pellets were diluted with astaxanthin phosphate duck egg yolk extender containing various concentration (4, 6, 8, 10, and 12%). Semen was packed with 0.5 mL straw and performed equilibration for 2 hours (4oC). do aeration above liquid N2 vapor for 10 minutes, then dipped in liquid N2. Semen was stored in liquid nitrogen container for 24 hours , and then quality of spermatozoa was evaluated post thawing. The results of the study showed that addition of cryoprotectan glycerol and DMSO were no difference significant (p>0,05) on the quality of frozen semen, but cryoprotectant concentrations (4%, 6%, 8%, 10%, and 12%) significantly affected (p <0.05) the quality of frozen semen. Glycerol with a concentration of 4% produces the best quality frozen semen, whereas DMSO with a concentration of 6% produces the best quality frozen semen.
Biokimia Darah Hati dan Ginjal Setelah Implan Wire SS316L dan Wire Alternatif (BLOOD BIOCHEMISTRY OF LIVER AND KIDNEY AFTER IMPLANT WIRE SS316L AND ALTERNATIVE) Erwin Erwin; Rusli Rusli; Amiruddin Amiruddin; Etriwati Etriwati; Muhammad Isa; Abdul Harris; Yula Astuti
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The blood biochemistry changes of liver and kidney are indicative of disorder in these organs. The objective of our experimental approach was to examine the level serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), blood urea nitrogen (BUN) dan creatinine after implant wire SS316L and alternative. Ten rats wistar strain aged 2-3-month-old, weighing 250-300 g were divide into two groups. Group I (G-I) implanted wire SS316L and group II (G-II) implanted alternative wire in os femur with each had a diameter of 0,5 mm. Blood sample was taken from sinus orbitalis on day 0 before implant, on day seven, 14, 21, 42 and 84 after implant. The results showed an increase in levels SGPT, SGOT, BUN and creatinine in the first few weeks after implantation. The levels of SGPT, SGOT, BUN and creatinine decreased again in the last week of observation with significant differences between the two treatment groups (P<0.05). The implant wire SS316L and alternative does not cause increased levels SGPT, SGOT, BUN dan creatinine for long time. Thus, it can be concluded that alternative wire has potential as internal fixation in fracture of small animal.
Respons Kekebalan Tubuh Ayam IPB D1 terhadap Infeksi Virus Penyakit Tetelo (Newcastle Disease) (IMMUNE RESPONSE OF IPB D1 CHICKEN AGAINST NEWCASTLE DISEASE VIRUS ) Retno Setyaningsih; Sri Murtini; Okti Nadia Poetri; Cece Sumantri
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Newcastle disease (ND) or Tetelo is an endemic poultry disease in Indonesia, and caused significant economic losses. Instead of diseases control programme has been carried out, ND outbreaks still occurs among poultry flock. IPB D1 chicken is a type of cross-breeding chickens between pelung-sentul crossbred chicken male and kampung-broiler crossbred chicken female, this chicken is genetically selected based on on body resistance to disease. The aim of our research is to study immune response of IPB D1 against ND infection. Fourty IPB D1 chickens were used in this study, and divided into three groups : vaccinated group, unvaccinated group, control group. Vaccinated group received two time ND vaccination at age 10 weeks and booster at age 12 weeks. Unvaccinated and control group were remain not vaccinated. At age 13 weeks, vaccinated and unvaccinated group were challenge with ND virus (107 EID50/ml). Immune response were determined based on antibody titer which is measured by haemagglutination inhibition (HI) test and the profile of white blood cell of the chicken. Our result showed that both vaccinated and unvaccinated group has ND antibody titer e” log 24 at challenge time, and survived after challenge. White blood cell profile among all groups showed that lymphocites has the higher number than other leukocyte type. It can be concluded that IPB D1 chickens showed a good response against ND virus.

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