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INDONESIA
The Indonesian Biomedical Journal
Published by The Prodia Education and Research Institute
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Public Health
Arjuna Subject : -
Articles 601 Documents
 3   4   5   6   7 
Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell-derived Secretome Induced Apoptosis and Inhibited Growth of HeLa Cells Ferry Sandra; Janti Sudiono; Elina Ardiani Sidharta; Elisabeth Pricilia Sunata; Dea Jane Sungkono; Yanni Dirgantara; Angliana Chouw
The Indonesian Biomedical Journal Vol 6, No 1 (2014)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v6i1.44

Abstract

BACKGROUND: Secreted factors contained in conditioned media (CM) of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) known as secretome, was suspected to have important roles in regulating cells. This study was conducted to investigate the role of CM-hUCB-MSCs-derived secretome in apoptosis and growth of HeLa cells.METHODS: HeLa cells were treated with secretome in various concentrations (0, 0.2, 2 and 20%) for 24 and 48 hours. Trypan blue exclusion assay was performed to detect cell viability. Meanwhile sub-G1 apoptotic assay was performed to detect apoptotic cells. The transition of mitochondrial transmembrane potential (TMP), which occurs in the apoptotic process, was analyzed by mitochondrial membrane potential (ΔΨM) assay. Both sub-G1 and ΔΨM assays were performed using FACSCanto flow cytometer. Statistical analyses were conducted using IBM SPSS Statistics to detect significance level at p<0.05.RESULTS: Secretome significantly induced cell death starting at concentration of 0.2% within a 24-hour period (p<0.05). Secretome significantly induced cell death in concentration and time dependent manner (p<0.05). The cell death was then confirmed as apoptosis through sub-G1 analysis. Due to the underlying apoptotic mechanism, we found distinct decrease of TMP, indicating an increase in mitochondrial membrane permeability of HeLa cells. In addition, we found that HeLa cell growth was inhibited partially by secretome.CONCLUSION: Taken together, we conclude that CMhUCB-MSCs-derived secretome significantly induced apoptosis of HeLa cells in a concentration and time dependent manner through mitochondrial apoptotic pathway. The secretome might also play important role in inhibiting HeLa cell growth.KEYWORDS: umbilical cord blood, mesenchymal stem cell, secretome, apoptosis, growth, cancer
Brucea javanica Leaf Extract Activates Caspase-9 and Caspase-3 of Mitochondrial Apoptotic Pathway in Human Oral Squamous Cell Carcinoma Muhammad Ihsan Rizal; Ferry Sandra
The Indonesian Biomedical Journal Vol 8, No 1 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i1.198

Abstract

BACKGROUND: We previously reported Brucea javanica leaf extract (BJLE) induced apoptosis in human oral squamous cell carcinoma (HSC2) cells by attenuation of mitochondrial membrane permeability. However, further underlying mechanism is not known yet. Therefore, we conducted a study to investigate activation of Caspases related to attenuation of mitochondrial membrane permeability in BJLE-treated human oral squamous cell carcinoma.METHODS: B. javanica leaves were collected, identified, minced, dried, extracted with distilled ethanol at room temperature for 24 hours, filtered and evaporated. Resulted BJLE was stored at 4°C. HSC-2 and HSC-3 cells were fasted for 12 hours and treated with BJLE in various concentrations for 24 hours. Treated HSC-2 and HSC-3 cells were lysed and subjected to western blot, to detect cleaved-Caspase-9, cleaved-Caspase-3 and β-actin. All visualized bands were captured and quantified.RESULTS: Low numbers and morphological alterations of adherent HSC-2 and HSC-3 cells were observed in the group of cells treated with 500, 100 and 10 μg/mL BJLE. Numbers of adherent HSC-2 and HSC-3 cells treated with BJLE were shown decreased along with the increase of BJLE concentrations. Meanwhile, numbers of floating HSC-2 and HSC-3 cells were increased. Bands of cleaved-Caspase-9 and cleaved-Caspase-3 were observed in HSC-2 and HSC-3 cells treated with 500 and 100 μg/mL BJLE. Higher-density bands of cleaved-Caspase-9 and cleaved-Caspase-3 were observed in HSC-2 and HSC-3 cells treated with 500 μg/mL BJLE than 100 μg/mL BJLE. CONCLUSION: BJLE could induce apoptosis by activation of Caspase 9 and Caspase 3 of mitochondrial apoptotic pathway in human oral squamous cell carcinoma. KEYWORDS: Brucea javanica, leaf, apoptosis, HSC-2, HSC-3, Caspase 9, Caspase 3
Optimization and Validation of a Real Time Reverse Transcriptase Polymerase Chain Reaction with RNA Internal Control to Detect Rubella RNA Winny Xie; Yusmiati Yusmiati
The Indonesian Biomedical Journal Vol 5, No 3 (2013)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v5i3.70

Abstract

BACKGROUND: According to a report from WHO, cases of rubella infection in Indonesia has increased up to 10-fold from 2007 to 2011. Despite no data of congenital rubella syndrome in the report, there are approximately 45,000 cases of babies born with heart failure and 0.1-0.3% live births with congenital deafness in Indonesia. Allegedly, rubella infection during pregnancy may play a role in this condition. This study aimed to optimize and validate a real-time reverse transcriptase polymerase chain reaction (RT-qPCR) method to detect rubella virus RNA as an aid for the diagnosis of congenital rubella infection.METHODS: Method optimization was conducted using nucleic acids extracted from Trimovax Merieux vaccine with the High Pure Viral Nucleic Acid Kit. One step RT-qPCR was performed with Quantifast Multiplex RTPCR+R Kit. Target synthetic DNA was designed and used to determine the sensitivity of the method. RNA internal control was synthesized to control the process of extraction and amplification.RESULTS: The analytical sensitivity of this method was as low as 5 copies target synthetic DNA/μl. The mean Coefficient of Variation (CV) % of the critical threshold (Ct) obtained were 2.71%, 1.20%, 1.62%, and 1.59% for within run, between run, between kit lots, and between operators, respectively. Recovery of the target synthetic DNA from amniotic fluid was 100.51% (by the log copies/μl) at the concentration of 1,000,000 copies/μl.CONCLUSION: RT-qPCR is successfully used for the detection of rubella virus RNA in vaccine and synthetic nucleic acid. With its high sensitivity, good precision and recovery, this method offers a means to improve the diagnosis of congenital rubella infection in developing countries like Indonesia.KEYWORDS: congenital rubella, RT-qPCR, prenatal diagnosis, amniotic fluid
The Stem Cell Hypothesis of Aging Anna Meiliana; Andi Wijaya
The Indonesian Biomedical Journal Vol 2, No 1 (2010)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v2i1.108

Abstract

BACKGROUND: There is probably no single way to age. Indeed, so far there is no single accepted explanation or mechanisms of aging (although more than 300 theories have been proposed). There is an overall decline in tissue regenerative potential with age, and the question arises as to whether this is due to the intrinsic aging of stem cells or rather to the impairment of stem cell function in the aged tissue environment.CONTENT: Recent data suggest that we age, in part, because our self-renewing stem cells grow old as a result of heritable intrinsic events, such as DNA damage, as well as extrinsic forces, such as changes in their supporting niches. Mechanisms that suppress the development of cancer, such as senescence and apoptosis, which rely on telomere shortening and the activities of p53 and p16INK4a may also induce an unwanted consequence: a decline in the replicative function of certain stem cells types with advancing age. This decrease regenerative capacity appears to pointing to the stem cell hypothesis of aging.SUMMARY: Recent evidence suggested that we grow old partly because of our stem cells grow old as a result of mechanisms that suppress the development of cancer over a lifetime. We believe that a further, more precise mechanistic understanding of this process will be required before this knowledge can be translated into human anti-aging therapies.KEYWORDS: stem cells, senescence, telomere, DNA damage, epigenetic, aging
Correlation of Ghrelin and Obestatin with Waist Circumference in Central Obese Men Widya Kurniawati; Marsetio Donosepoetro; Andi Wijaya
The Indonesian Biomedical Journal Vol 3, No 2 (2011)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v3i2.144

Abstract

BACKGROUND: Central obesity is known as the cause of many metabolic disorders called Metabolic Syndrome. Accumulation of adipocytes in central obesity increases production of cytokines proinflammation. Free fatty acid increases in obesity that drives atherogenic dyslipidemia and insulin resistance. IDF 2005 states that waist circumference (WC) is regarded as the simple criteria of obesity. Energy imbalance lasting for a long period is a determinant factor for obesity, e.g. when energy intake is greater than energy expenditure. The brain and gastrointestinal tract work together to maintain this system. Ghrelin and Obestatin are two gut hormones that work in different ways to keep the energy balance. Ghrelin increases appetite but Obestatin decreases it. The two hormones play an important role in maintaining the dynamic equilibrium of energy balance. This study was aimed to determine correlation of Ghrelin and Obestatin with WC in central obese men.METHODS: This was a cross sectional study involving 53 central obese men. Based on IDF 2005 central obesity is most easily measured by waist circumference using the guidelines ethnic group (not country of residence) specific. We used South Asia ethnic which including Chinese, Malay and Asian Indian population as criteria for this study, that was WC >90 cm, aged 20-60 years. Subjects who had smoking habit, any infectious disease, and ACS were excluded from the study. No restriction was applied on the kind of meals the subjects were having or activities they were doing. The correlation of waist circumference with ghrelin and obestatin was assessed with a significance level of 95% (α=0,05).RESULTS: Patient's age was 40.9623±7.9080 year, waist circumferences was 102.1981±10.2696 cm, weight was 85.8679±16.5475 kg, height was 168.8066±6.3535 cm, BMI was 29.9723±2.4937 kg/m2. Concentration of Ghrelin were 0.70-13.72 ng/mL, and Obestatin 16.66-148.84 pg/mL. Pearson correlation showed that Ghrelin (r=-0.1114, p=0.4271) and Obestatin (r=-0.1781, p=0.2020) had no significant correlation with WC. But in patients WC ≥120 cm had significant negative correlation with Obestatin (r=-0.375, p=0.049).CONCLUSIONS: There was no significant correlation of Ghrelin and Obestatin with WC in obese men. However, there was a negative correlation tendency found in patients with greater WC (≥102 cm).KEYWORDS: obesity, ghrelin, obestatin, waist circumference (WC)
Proliferation of Peripheral Blood-derived Endothelial Progenitor Cells from Stable Angina Subjects Yudi Her Oktaviono; Djanggan Sargowo; Mohammad Aris Widodo; Yanni Dirgantara; Angliana Chouw; Ferry Sandra
The Indonesian Biomedical Journal Vol 6, No 2 (2014)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v6i2.34

Abstract

BACKGROUND: A population of circulating Endothelial Progenitor Cells (EPCs) has been reported to play important role in maintaining endothelial function and integrity. Since EPCs culture is crucial and an optimized medium is currently available. Therefore we conducted a study to investigate whether stable angina subjects peripheral blood-derived EPCs could be cultured in this medium. Here, we performed study to detect EPCs characteristics and extracellular signalregulated kinase (Erk)1/2 Mitogen-Activated Protein Kinase (MAPK) pathway as possible underlying pathway for EPCs proliferation.METHODS: Peripheral blood EPCs from 8 stable angina subjects were cultured in an optimized medium with/without addition of supplement for 1 or 3 days. Then, the membrane of cultured EPCs were detected with immunofluorescence method for CD34, Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2) and CD133. Colony forming unit (CFU) enumeration was performed. XTT Cell proliferation assay was performed to assess EPCs growth after 1 and 3-days culture. The western blot analysis was performed to detect possible activation of Erk1/2 MAPK.RESULTS: Number of EPCs and CFU cultured for 3 days were significantly higher than the ones cultured for 1 day (p=0.012). EPCs membrane markers from stable angina subjects were detected as well as CFUs were formed. There were significant increase of EPCs number, CFUs number and phosphorylated-Erk2 amount when the groups with and without supplement were compared (p<0.05). Meanwhile U0126, a MAPK Erk1/2 (MEK1/2) inhibitor, significantly inhibited the supplement-induced EPCs number, CFUs number and phosphorylated-Erk2 amount (p<0.05).CONCLUSION: Our results showed that ERK2 MAPK signaling pathway might play an important role in supplement-induced peripheral blood EPCs proliferation in subjects with stable angina.KEYWORDS: endothelial progenitor cell, EPC, p42, Erk2, proliferation
Chronodisruption and Obesity Anna Meiliana; Nurrani Mustika Dewi; Andi Wijaya
The Indonesian Biomedical Journal Vol 7, No 3 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i3.184

Abstract

BACKGROUND: Attempts to understand the causes of obesity and develop new therapeutic strategies have mostly focused on caloric intake and energy expenditure. Recent studies have shown that the circadian clock controls energy homeostasis by regulating circadian expression and/or activity of enzymes, hormones, and transport systems involved in metabolism. Moreover, disruption of circadian rhythms leads to obesity and metabolic disorders.CONTENT:Regularly alternating periods of light and darkness, such as normally occur with the rising and the setting of the sun, are essential for the maintenance of undisturbed circadian rhythms in all organisms including humans. The light-dark environment, as detected by specialized photoreceptors in the retinas, impacts the endogenous circadian clock in the anterior hypothalamus, the suprachiasmatic nuclei. These nuclei, via both neural and humoral signals, communicate with cells throughout the organism to establish regular circadian rhythms. The introduction of artificial sources of light roughly 150 years ago has significantly undermined the naturally occurring light-dark environment and, likewise, has disturbed circadian rhythms since light is now available at unusual times, i.e., at night. Light at night is known to cause circadian disruption and melatonin suppression. Many potentially pathophysiological consequences of these artificial light-mediated changes, include cancer, cardiovascular diseases, insomnia, metabolic syndrome, diabetes, and cognitive disorders may be aggravated by the increased exposure to light at night, which is inevitable in well-developed societies that have undergone extensive electrification.SUMMARY: Therefore, it is plausible that resetting of the circadian clock can be used as a new approach to attenuate obesity. Feeding regimens, such as restricted feeding, calorie restriction and intermittent fasting, provide a time cue and reset the circadian clock and lead to better health. In contrast, high-fat diet leads to disrupted circadian expression of metabolic factors and obesity.KEYWORDS: obesity, circadian clock, metabolism, chronodisruption
The Correlation between Glycemic Characteristic and Erythrocyte Indices in Obesity Dharma Lindarto; Santi Syafril; Dairion Gatot
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.215

Abstract

BACKGROUND: Elevated blood glucose level is a major factor in development of diabetic complications due to unfavorable hyperglycemic induced biochemical as well as hematological indices changes. The aim of this study was to evaluate the correlation between glycemic characteristic and erythrocyte indices in obese subjects with different glycemic status.METHODS: Cross cross-sectional study was designed, and 80 obese subjects were enrolled. The correlations between glycemic characteristic (fasting plasma glucose (FPG), postprandial plasma glucose (PPG), hemoglobin A1c (HbA1c) and homeostasis model assessment of insulin resistance (HOMA-IR)) and erythrocyte indices (Hb, red blood count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC)) were evaluated.RESULTS: Of 80 obese subjects with different glycemic status, there were 48 patients with only obesity (HbA1c <5.7%), 19 patients with pre-diabetes (HbA1c 5.7-6.4%) and 13 patients with diabetes (HbA1c >6.4%). Glycemic characteristic and profile lipid (high-density lipoprotein cholesterol (HDL-C), triglycerides (TG)) were differ significantly in the different HbA1c level. Erythrocyte indices were not differ significantly in the different HbA1c level. Partial Spearman's correlation analysis showed that only MCV was significantly correlated with glycemic characteristic of FPG, PPG, HbA1c and HOMA-IR (r=-0.36, p=0.001; r=-0.29, p=0.007; r=-0.27, p=0.014 and r=-0.236, p=0.035; respectively).CONCLUSION: MCV was significantly correlated with glycemic characteristic (FPG, PPG, HbA1C and HOMAIR). Further investigations are recommended.KEYWORDS: MCV, glycemic-characteristic, HbA1c, erythrocyte indices
Adipose Tissue Biology: An Update Review Anna Meiliana; Andi Wijaya
The Indonesian Biomedical Journal Vol 1, No 3 (2009)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v1i3.98

Abstract

BACKGROUND: Obesity is a major health problem in most countries in the world today. It increases the risk of diabetes, heart disease, fatty liver and some form of cancer. Adipose tissue biology is currently one of the “hot” areas of biomedical science, as fundamental for the development of novel therapeutics for obesity and its related disorders.CONTENT: Adipose tissue consist predominantly of adipocytes, adipose-derived stromal cells (ASCs), vascular endothelial cells, pericytes, fibroblast, macrophages, and extracellular matrix. Adipose tissue metabolism is extremely dynamic, and the supply of and removal of substrates in the blood is acutely regulated according to the nutritional state. Adipose tissue possesses the ability to a very large extent to modulate its own metabolic activities including differentiation of new adipocytes and production of blood vessels as necessary to accommodate increasing fat stores. At the same time, adipocytes signal to other tissue to regulate their energy metabolism in accordance with the body's nutritional state. Ultimately adipocyte fat stores have to match the body's overall surplus or deficit of energy. Obesity causes adipose tissue dysfunction and results in obesity-related disorders.SUMMARY: It is now clear that adipose tissue is a complex and highly active metabolic and endocrine organ. Undestanding the molecular mechanisms underlying obesity and its associated disease cluster is also of great significance as the need for new and more effective therapeutic strategies is more urgent than ever. KEYWORDS: Obesity, Adipocyte, Adipose, Tissue, Adipogenesis, Angiogenesis, Lipid Droplet, Lipolysis, Plasticity, Dysfunction  
Lipoprotein (a) and Lipoprotein-associated Phospholipase A2 as Atherosclerosis Risk Factors (oxLDL) in Men with Central Obesity Nelly Sari; Andi Wijaya; Ilhamjaya Patellongi
The Indonesian Biomedical Journal Vol 3, No 1 (2011)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v3i1.134

Abstract

BACKGROUND: The increasing prevalence of obesity in Indonesia triggers a lot of research interest to overcome it. Obesity has a very important role as atherosclerosis and cardiovascular risk factors. The presence of oxidized LDL (oxLDL) on the vascular wall is a marker of atherosclerosis. The increase of Lipoprotein(a) (Lp(a)) and Lipoprotein associate phospholipase A2 (LpPLA2) occurs in patients with coronary artery disease (CAD), myocardial infarction, and unstable angina. It is well accepted that obesity is closely related to atherosclerosis and cardiovascular risk factors. However, correlation between Lp(a), LpPLA2 and oxLDL in central obesity has not yet been investigated. The aim of this study was to observing the correlation between Lp(a), LpPLA2 and oxLDL in early central obesity.METHODS: An observational study with cross-sectional design on 76 men with central obesity, aged 30-67 years, was conducted. Central obesity was characterized by waist circumference >90 cm. Test of Lp(a) was performed by turbidimetric method and that of LpPLA2 was performed by sandwich enzyme immunoassay. Test of oxLDL was performed by ELISA. All statistical analyses were carried out using SPSS for Windows v.11.5 at a significance level of p<0.05. The Pearson and Spearman’s Rho correlation coefficient was used to assess the correlation between Lp(a), LpPLA2 and oxLDL. Obese men with acute inflammation (hsCRP > 10 mg/L), renal failure (Creatinine >1.5 mg/dL) and consumed antiinflammation were excluded from this study.RESULTS: The concentration of LpPLA2 had a linear correlation (r=-0.340, p=0.003) with the increase of oxLDL concentration. However, concentration of Lp(a) did not have linear correlation (r = 0.025) with increase of oxLDL concentration. This finding indicates that concentration of LpPLA2 had a negative correlation with increase of concentration of oxLDL. In addition, Lp(a) appears not to correlate with oxLDL significantly.CONCLUSION: The study showed there was a significant correlation between concentration of LpPLA2 and concentration of oxLDL in men with central obesity. Higher concentration of LpPLA2 correlated with lower concentration of oxLDL.KEYWORDS: Lp(a), LpPLA2, oxLDL, atherosclerosis, central obesity

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