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Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "Vol 13 No 1 (2009): JURNAL BIOLOGI" : 6 Documents clear
PEMANFAATAN BAHAN ORGANIK SEBAGAI BAHAN PEMBAWA INOKULAN FUNGI MIKORIZA ARBUSKULA ANNE NURBAITY; DIYAN HERDIYANTORO; OVIYANTI MULYANI
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Arbuscular Mycorrhizal Fungi (AMF) is known as one type of biofertilizer. The carrier for this biofertilizer is usually made from inorganic material such as zeolite. Currently, it is important to find the alternative materials that can be used as biofertilizers’ carrier due to the need of lower cost and easily available, such organic matter. Rice straw and husk are some of the organic matter sources that can be used as a carrier of AMF. A glass house experiment was conducted to determine the effectiveness of different type of organic matter as a carrier of AMF’s inoculum. The experiment was arranged in factorial randomized block design with two factors, i.e. type of organic matter (zeolite as a control, straw, burnt-rice’s husk and combination of straw and rice husk 50/50 v/v) and type of different hosts of AMF’s (Jatropha sp. and Sorghum sp.). The results showed that application of burnt-rice’s husk was better carrier of AMF inoculum instead of straw or combination of straw and burnt-rice’s husk. The quality of AMF inoculum with burnt-rice’s husk as a carrier was as good as the control inoculum that used zeolite, in terms of the number of spores, the percentage of root colonization, root length colonized and root fresh weight. In summary, burnt-rice’s husk has a good potential carrier of AMF biofertilizer.
PENGARUH PADAT PENEBARAN TERHADAP PERTAMBAHAN BERAT DAN PANJANG BADAN BELUT SAWAH (MONOPTERUS ALBUS) I GUSTI KETUT ALIT
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This experiment performed to find out the effect of stocking density on body weight and body length of freshwater eel (Monopterus albus). In this experiment 160 individual freshwater eels that planted in 20 clay pots. Completely Randomized Design (CRD) with five treatments and four replicates applied in this experiment. The treatments of different stocking densities were 4 eels/pot, 6 eels/pot, 8 eels/pot, 10 eels/pot, 12 eels/pot. Significant gains were recorded on body weight (P0,05) for different stocking densities.
ISOLASI STREPTOMYCES SPP. PADA KAWASAN HUTAN PROVINSI BALI SERTA UJI DAYA HAMBATNYA TERHADAP LIMA STRAIN DIARRHEAGENIC ESCHERICHIA COLI I WAYAN EKA DHARMAWAN; MADE SUSUN PARWANAYONI; RETNO KAWURI
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

An exploration study of natural resources soil bacteria antibiotic-producer, Streptomyces spp. was done in two steps. The first step was isolation of Streptomyces and the second involved testing their inhibition activities against five strains diarrheagenic Escherichia coli. Soil samples were collected from ten forest areas in Bali. As many as 55 isolates were collected with various macroscopic dan microscopic characters. Most isolates (eight Streptomyces isolates) were collected from forest area in Penulisan, Kintamani (RTK. 20). The diversities of isolates are influenced by environment condition. All Streptomyces isolated were tested against five strains diarrheagenic Escherichia coli to check antibiotic activity for inhibit growth of E. coli. Streptomycine was used as a control. The result showed that the largest inhibition zones of Streptomyces against E. coli strains EHEC, ETEC, EIEC, EPEC and DAEC were produced by Streptomyces PK5 (48,67 ± 0,58 mm), Streptomyces GAA4 (29,00 ± 2,00 mm), Streptomyces GBK3 (42,67 ± 2,08 mm), Streptomyces SkBB5 (29,00 ± 2,65 mm) and Streptomyces GM3 (33,67 ± 3,21 mm) respectively.
PENENTUAN KINETIKA ENZIM POLIGALAKTURONASE (PG) ENDOGENOUS DARI PULP BIJI KAKAO G.P. GANDA PUTRA
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research was conducted to determine the enzyme kinetics (Km and Vmax) of endogenous polygalacturonase (PGs) isolated from cocoa pulp. This research was carried out by assaying PGs activities in various concentration of citrus pectin subtrates which were between 0,1 – 1,0% with 0,1% interval. The result showed that the enzyme kinetics of endogenous PGs from cocoa pulp (Km) was 0,37% and Vmax was 6,69 µmol of galacturonic acid/minute/ml.
APLIKASI PENGGUNAAN ZPT PADA PERBANYAKAN RHODODENDRON JAVANICUM BENN. (BATUKAU, BALI) SECARA VEGETATIF (SETEK PUCUK) DYAN M.S. PUTRI; I NYOMAN SUDIATNA
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Rhododendron javanicum Benn. has a potential use as an ornamental plant and medicine. The aim of the research was to find out the effect of plant growth regulator on the vegetative growth of Rhododendron javanicum Benn. (Batukau, Bali). The experiment was designed in Cluster Random Sampling, which the groups I consist of concentrations of plant growth regulators (Rootone F: 0 ppm (control), 100 ppm, 150 ppm, 200 ppm). Sampling time treatment of 30, 60, 90 and 120 days after planted were used for groups II. Each treatment was replicated three times. The life materials used in this experiment were shoot cuttings of Rhododendron javanicum Benn. (Batukau, Bali) with equal numbers of leaves (5-7 leaves) and were planted in the pot using media types of Asplenium roots: Cyathea roots:charcoal (2:2:1). Observed parameters were the numbers of shoots, plants height and the environmental factors (temperature and humidity). Analysis of variance (the numbers of shoots) showed that for each concentration the number of shoots was differ significantly between time treatments. The variant analysis of plant heights showed that for each concentration the plant height was not differ significantly between time treatments. The temperature during the research is 15,31 – 23,76 oC and humidity is 56,20 – 85,34 %. From this research, it can be concluded that the vegetative growth of Rhododendron javanicum Benn. (Batukau, Bali) was influenced by time treatment but not by concentration of plant growth regulator. For better results it is suggested to increase the concentrations of plant growth regulator.
OPTIMALISASI EKSTRAKSI DNA DAN PCR-RAPD PADA GREVILLEA SPP. (PROTEACEAE) MADE PHARMAWATI
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Molecular genetic analysis of plants relies on high yield and high purity of DNA as well as optimized condition of molecular reactions. Appropriate methods for DNA extraction and molecular reactions such as PCR are therefore needed. This study aimed to develop protocol for extraction of high molecular weight DNA from Grevillea leaf and to optimize condition of PCR-RAPD. Standard plant DNA extraction of Doyle and Doyle was modified by increasing EDTA concentration to 50 mM and addition of 2% (v/v) 2-mercaptoethanol. Moreover, incubation time was prolonged to 14-16 h at 55oC. This method yielded good quality of DNA and consistent results. Amplification of DNA using PCR-RAPD will become efficient and consistent if the amplification reactions are in ideal condition. In Grevillea, clear, reproducible and scorable PCR-RAPD patterns were obtained using 10ng DNA template, 5 pmol primer, 2.5 mM MgCl2 and the number of thermal cycle was 40 x.

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