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Isolation and molecular identification of fungi causing stem rot disease in Bali's local legumes Ni Made Susun Parwanayoni; Dewa Ngurah Suprapta; Nyoman Darsini; Sang Ketut Sudirga
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.20426

Abstract

Efforts to improve food security in Indonesia, particularly Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Meanwhile, legume crops are often attacked by stem rot diseases which potentially decrease production and leads to economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of these two species are the same and difficult to distinguish, hence, molecular identification is needed to differentiate between the species. Therefore, this study aims to isolate and molecularly identify the fungi causing stem rot disease in local legume plants in Bali. The methods used include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease, pathogenicity test, identification of isolates with the highest virulent levels, DNA extraction, DNA amplification by PCR and electrophoresis, ITS region sequencing and computer analysis sequences. The isolation procedure identified six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate as indicated by the pathogenicity test of peanut plants. Furthermore, molecular identification results show that SKT isolate is Athelia rolfsii, a similar clade with the fungi sequences in GenBank with 100% bootstrap support.
MENINGKATKAN PRODUKSI BUNGA POTONG ANTHURIUM SP MELALUI PEMANFAATAN PUPUK ORGANIK N. L. Suriani; N. M. S. Parwanayoni; N. M. Suartini
Buletin Udayana Mengabdi Vol 15 No 2 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

The aim of this study was to look at the effectiveness of organic fertilizers in increasing the production of anthurium sp flowers. Method of this research a randomized block design with 4 treatments and 6 replications. Starting with the manufacture of organic fertilizers by using chicken dung and waste cut flowers. Then made an application to the anthurium flowers. Parameters measured were plant height, number of leaves, number of tillers / hill, the amount of interest / clump and flower diameter. The results that the treatment significantly different (P <0.05) with the control. This suggests that organic fertilizers can increase production of cut flower anthurium sp, with the highest amount of interest and the interest of the longest diameter contained in the fertilizer treatment of 1.5 tons / ha. Number of tillers / clump and number of leaves contained in the fertilizer treatment of 2 tons / ha, this is due to the treatment, the number of N fertilizer is too high that the increased leaf production. From the results of this study concluded that organic fertilizers can increase production of flower anthurium sp.
PERGANTIAN POPULASI BAKTERI HETEROTROF, ALGAE DAN PROTOZOA DI LAGOON BTDC UNIT PENANGANAN LIMBAH NUSA DUA BALI Ni Made Susun Parwanayoni
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

This research was carried out at Nusa Dua Lagoon, Nusa Dua – Bali in the year of 2006. The research was aimed to find out the concentration of heterotrophic bacteria, algae and protozoa, as well as to observe the change of the population in the lagoon. Results of this study showed that the highest concentration of the population of heterotrophic bacteria was on pond 4b (aerated pond 2), algae population was highest at pond 5a (sedimentation pond 1), and the population of protozoa was highest on pond 5b (sedimentation pond 2). The population of heterotrophic bacteria was replaced by algae and then algae was taken over by protozoa.
JENIS TUMBUHAN DAN KONDISI HUTAN MANGROVE DI NUSA LEMBONGAN DAN NUSA CENINGAN, KECAMATAN NUSA PENIDA, KABUPATEN KLUNGKUNG I G. A. Sugi Wahyuni; N. M. Susun Parwanayoni; Joko Wiryatno
Bumi Lestari Journal of Environment Vol 7 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

This research was undertaken in Nusa Lembongan and Nusa Ceningan, District ofNusa Penida, Klungkung Regency, between 2005-2006. This reserach was aimed to find outthe size of area and the status of mangrove forest, kind of species, as well as problems facedby the forest related to its conservation status. From this research it was revealed that thesize of the area where the mangrove available in Nusa Lembongan was around 212 ha, andthere was also mangrove forest in Nusa Ceningan about 15 ha. There were 13 species offlora available on mangrove ecosystem which were classified as true mangroves, while 9kinds were asociated mangroves. From the field observation it was revealed that there is apossible threat to the mangrove forest if there is no good management strategy. Someactivities which were available on the mangrove forest that were potentially provided impactsto sustainability of the forest, such as the use for anchoring boats and mangrove touractivities.
Daya Hambat Ekstrak Aseton Daun Jeringau (Acorus Calamus L.) Terhadap Pertumbuhan Jamur Sclerotium Rofsii Ni Made Susun Parwanayoni; Nyoman Darsini
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p2

Abstract

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Daya Hambat Ekstrak Aseton Daun Jeringau (Acorus Calamus L.) Terhadap Pertumbuhan Jamur Sclerotium Rofsii Ni Made Susun Parwanayoni; Nyoman Darsini
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p2

Abstract

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