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JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

JHPT TROPIKA
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Published by Universitas Lampung

ISSN : 14117525 EISSN : 24610399 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.

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STUDI LALAT PENGOROK DAUN LIRIOMYZA SPP. PADA PERTANAMAN BAWANG DAUN, DAN PARASITOID OPIUS CHROMATOMYIAE BELOKOBYLSKIJ & WHARTON (HYMENOPTERA: BRACONIDAE) Rusli Rustam, Aunu Rauf, Nina Maryana, Pudjianto, dan Dadang.
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 9 No. 1 (2009): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Studies on Leafminer Liriomyza spp. in Green Onion Fields, and Parasitoid Opius chromatomyiae Belokobylskij & Wharton (Hymenoptera: Braconidae). Field studies were conducted to determine population abundance of leafminers and their parasitoids in green onion fields in Puncak, West Java. In addition to that, laboratory studies were carried out to determine demographic parameter of Opius chromatomyiae as well as response of parasitoid to increasing host density. Results revealed that green onions were infested by two species of leafminers, Liriomyza huidobrensis and Liriomyza chinensis. Leafminer flies emerged from Erwor leaves (54.5) were significantly higher than those of RP leaves (18.65) (P = 0.0005). However, number of leafminer flies caught on sticky traps was not statistically different (P = 0.297). Two species of parasitoid, Hemiptarsenus varicornis and O. chromatomyiae, were associated with leafminers in green onion fields. Higher number of parasitoids emerged from Erwor leaves (13.68) as compared to RP (6.90) (P =0.0007 ). However, level of parasitization were 24.36% on Erwor and 28.45% on RP, and was not significantly different (P = 0.387). Laboratory studies indicated that net reproduction (Ro) of O. chromatomyiae was 28.55, generation time (T) 15.96 days, intrinsic growth rate 0.21, and total of reproductive value 223.64. The stable age distribution of parasitoid were 37.93% eggs, 24.92% larvae, 20.36% pupae and 16.78% adults. The parasitoid showed functional response type II to increasing host density, with a = 0.08 and Th = 2.58.

PENGARUH WAKTU INFEKSI VIRUS KERDIL PISANG TERHADAP KERENTANAN TIGA KULTIVAR Dewi Widyastuti dan Sri Hendrastuti Hidayat .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 1 (2005): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Effects of time of infection of banana bunchy top virus on susceptibility of three banana cultivars. Banana Bunchy Top, caused by Banana Bunchy Top Virus (BBTV), is one of the most important banana diseases in Indonesia. Approach to reduce disease incidence involves prevention of early infection especially on susceptible cultivars. This study was conducted to evaluate the response of three banana cultivars, Ambon Kuning, Tanduk, and Kepok, to different time of infection of BBTV i.e., one week and three week after adaptation period, and one week during adaptation period. Banana plants used in the study were prepared through in vitro propagation (tissue culture) and virus transmission was done using aphid vector, Pentalonia nigronervosa. In addition to observation on symptom expression, inhibition of plant height, and reduction of leaf size, conformation of virus infection was done through indirect ELISA. Virus concentration on different part of the plant, young leaf, stem, and root, tends to decrease over the time due to the ability of BBTV to move from cell to cell before replication takes place. It is evidenced that BBTV was able to infect banana in all growth stages although the younger plant is more susceptible to BBTV. Although concentration of the virus in the tested plant is considered high, symptoms expression of BBTV infection can be differentiated from moderate to very severe. Response of banana plants to infection of BBTV can be grouped into susceptible (Ambon Kuning), moderate tolerant (Tanduk), and tolerant (Kepok).

SINERGISME CENDAWAN ENTOMOPATOGEN Lecanicillium lecanii DENGAN INSEKTISIDA NABATI UNTUK MENINGKATKAN EFIKASI PENGENDALIAN TELUR KEPIK COKLAT Riptortus linearis PADA KEDELAI Yusmani Prayogo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The aim of the research was to study the synergism of entomopathogenic fungi Lecanicillium lecanii with botanical insecticides to control brown stink bug. The research was carried out in the field experiment station (Kendalpayak) of Indonesian Legume and Tuber Crops Research Institute (ILETRI). Randomized block design (RBD) was used, each treatment was repeated three times. The treatments were three types of botanical insecticides i.e; Aglaia odorata leaf powder (ALP), Annona squamosa seed powder (ASP), and Jatropha curcas seed powder (JSP) of 25, 50 and 75 g/l respectively were combined with the conidia of L. lecanii. The results showed that the addition of botanical insecticides into the media can improve growth and development of the fungus L. lecanii. The higher dose of botanical insecticide in combination with the L. lecanii, the greater efficacy of both agents are in controlling brown stink bug eggs. Synergism of combination of L. lecanii and botanical insecticides were shown from the number of unhatched eggs (above 77%) compared with a single application. The fewer number of brown stink bug eggs hatching, caused the limited number of brown stink bug can developed become adult, resulting reduced soybean seeds and pods damaged. The combination of L. lecanii and botanical insecticides was able to maintain weight of soybean seed up to 35% compared to single treatment (control). The combination of L. lecanii and ASP and JSP botanical pesticides were more synergist compared with combination of L. lecanii with ALP. Dose of 50 g/l botanical pesticide JSP and ALP were combined with the L. lecanii was an optimal dose to control of brown stink bug eggs.

EFIKASI CENDAWAN ENTOMOPATOGEN LECANICILLIUM LECANII TERHADAP BEMISIA TABACI (HEMIPTERA: ALEYRODIDAE) PADA KEDELAI Yusmani Prayogo .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Efficacy of entomopathogenic fungi Lecanicillium lecanii to Bemisia tabaci (Hemiptera: Aleyrodidae) on Soybean. Bemisia tabaci is one of the most important pests on soybean, because in addition to direct damage also as vectors of viruses and stimulated the growth of sooty mold. The efficacy of entomopathogenic fungi Lecanicillium lecanii to control B. tabaci carried out at Muneng field research station of Probolinggo in June until September 2012. Research using a split plot design, the main plot is two soybean varieties, namely Argomulyo and Wilis. Subplot is the time application of fungi L. lecanii with conidia densities of 107 and 109/ml. The results showed that Argomulyo more preferred by B. tabaci compared Wilis variety. Nine times application of L. lecanii using 107/ml conidia densities were applied every week the started on 14-70 days after planting (DAP) was able to suppress of B. tabaci in the field. However, planting Wilis varieties with nine times application of L. lecanii using conidia densities 109/ml were recommended. Controlling of B. tabaci on Argomulyo variety use L. lecanii or lamda sihalotrin showed no significantly due to the result seed weight is lower than compare with Wilis untreated. Application of L. lecanii use with the highest conidia densities (109/ml), no negative effect to the survival on survival of various predators; i.e. Coccinella sp., Oxyopes sp. and Paederus sp. Therefore, L. lecanii most likely to be used as an effective biopesticide agent to control of B. tabaci and as substitute to chemical insecticides.

UJI PENGGABUNGAN PGPF DAN Pseudomonas putida STRAIN PF-20 DALAM PENGENDALIAN HAYATI PENYAKIT BUSUK LUNAK LIDAH BUAYA DI TANAH GAMBUT . Supriyanto; Achmadi Priyatmojo; Triwidodo Arwiyanto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Aloe (Aloe vera L. Webb) planted in West Borneo peat land is well known to have the best product quality in Indonesia. Bacterial soft rot is one of the constraints on aloe cultivation on peat land. Many methods have not given significant result for controlling this disease. The research objectives were to study the application of PGPF and its combination with Pseudomonas putida Pf-20 for controlling aloe bacterial soft rot on peat land. In vitro test showed that two isolates of PGPF tested had different respons when combining with P. putida Pf-20. The bacterial P. putida Pf-20 inhibit the growth of PGPF SNTH003 (Aspergillus sp.) as 20.14% on King’s B and 7.48% on PDA, whereas SNTH001 (Penicillium sp.) as 62.4% on King’s B dan 34.39% on PDA. Glass house trial showed that root dipping in P. putida Pf-20 suspension could not promote the growth of aloe, but could reduce the disease intensity. The single application of SNTH001 and SNTH003 isolates were able to increase the growth of aloe compare with its combination each of PGPF isolates with P. putida Pf-20. Single application of SNTH003, SNTH001 and P. putida Pf-20 was able to reduce the disease intensity of bacterial soft rot, but the capability decreased when each of PGPF combined with P. putida Pf-20. However combination between SNTH003 and P. putida Pf-20 increased that disease intensity. The lowest disease intensty (25%) obtained by using SNTH001 isolate.

KEEFEKTIFAN LIMBAH TANAMAN BRASSICACEAE UNTUK PENGENDALI NEMATODA PURU AKAR (MELOIDOGYNE SPP.) PADA MIKROPLOT DI LAPANGAN Muhammad Jabal Nur; Supramana .; Abdul Munif
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 16 No. 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Effectivenes of Brassicaceae plant wastes to control the root knot nematodes (Meloidogyne spp.) at a field microplotÂ scale. Meloidogyne spp. is a soil borne pathogen that infects plant roots and causes root galls. Root knot nematodes can reduce crop production by 15 to 95%, so that the control measures are needed. One of the control methods is using plants as biofumigant. Plants of the family Brassicaceae were reported contain glucosinolate (GSL). During decomposition, GSL is hydrolized to isothiocyanates (ITS) which is a highly toxic compound to soil organisms, including nematodes. The research objective was to determine the effectiveness of five Brassicaceous plant wastes, namely cabbage (B. oleracea var capitata), radish (Raphanus sativus), broccoli (B. oleracea var italica), chinese cabbage (B. chinensis) and pakcoy (B. rapa varÂ parachinensis) to suppress root knot nematodes (RKN). The experiment was conducted on microplot scale in the field. The experimental design used was a 4x5 factorial CRD. The first factors are waste of Brassica and the second factors are the amount of Brassica wastes per microplot. The experiments were made in 6 replications. Application of 5 Brassica plant wastes at all doses tested effectively reduced the number of root knot by 45.65% to 94.43% and increased the average number of tomato fruits. Tomato plants grew better at microplots when treated with chinese cabbage and pakcoy wastes.

Aktivitas Antivirus Beberapa Ekstrak Tanaman terhadap Bean Common Mosaic Virus strain Black Eye Cowpea (BCMV-BIC) pada Kacang Panjang Tri Asmira Damayanti; Martha Theresia Panjaitan
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

ABSTRACTAntivirus actitivity of several plant extracts against Bean common mosaic virus strain Black eye cowpea (BCMV-BlC) on Yard long bean. Bean common mosaic virus (BCMV) is an important virus on yard long bean and it is difficult to control. One of control effort way by utilizing antiviral substances of plant origin. The research was done to select and test the effectiveness of plant extracts in suppressing BCMV infection on yard long bean. Twenty two plant extracts were selected by (1) spraying the crude extract to Chenopodium amaranticolor leaves, then plant inoculated by BCMV 1 hour after spraying, and (2) mixturing the crude extract with sap containing BCMV, then inoculated mechanically to C. amaranticolor. Local necrotic lesion number and inhibition percentage are measured. All plant extract treatments were able to reduce Necrotic lokal lesion formation significantly compared to untreatment control. Further, fifteen plant extracts were selected to test their effectiveness in controlling BCMV on yard long bean in green house trial. The results showed that except geranium and red ginger treatment, other extract treatments were able to reduce significantly the disease incidence and severity, symptoms, and BCMV titer, respectively. Among tested extracts, Bougainvillea spectabilis, Mirabilis jalapa, and Celosia cristata are the most effective crude extracts in suppressing BCMV infection.

DETEKSI KEBERADAAN CITRUS VEIN PHLOEM DEGENERATION (CVPD) DENGAN TEKNIK PCR (POLYMERASE CHAIN REACTION) DI SULAWESI TENGGARA Muhammad Taufik, Andi Khaeruni Terry Pakki1 & Gianto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Detection of ocurrence Citrus Vein Phloem Degeneration (CVPD) with PCR (Polymerase Chain Reaction) technique in Southeast Sulawesi. CVPD disease incidence in Southeast Sulawesi based on symptoms have never been reported. PCR (polymerase chain reaction) technique was utilized to detect CVPD with specific primer forward OI and reverse OI2, specific for amplication of Liberobacter asiaticum DNA. The result of the reseach indicated that 69% of orange trees in Ladongi, Kolaka District and 65% of orange trees in Lalembuu 1, Konawe Selatan district showed the symptom. PCR technique successfully amplified DNA of bacterium L. asiaticum with the size of 1100 bp.

TRANSGENE IDENTITY AND NUMBER OF INTEGRATION SITES AND THEIR CORRELATION WITH RESISTANCE TO PStV IN TRANSGENIC PEANUTS CARRYING PEANUT STRIPE VIRUS (PStV) COAT PROTEIN GENE Dwi Hapsoro, Hajrial Aswidinnoor, Rusmilah Suseno, Jumanto, and Sudarsono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Transgene Identity and Number of Integration Sites and Their Correlation with Resistance To PStV in Transgenic Peanuts Carrying Peanut Stripe Virus (PStV) Coat Protein Gene. This research aimed to determine (1) the identity and copy number of PStV cp gene in transgenic peanut plants carrying PStV cp gene and (2) correlation between the identity and the number of integration sites and resistance to PStV infection. One T0 transgenic peanut was selfed up to five generations. T2, T3, and T5 plants were mechanically inoculated with PStV. Samples of T5 plants derived from several different T4 plants were subjected to Southern analysis to confirm the integration of PStV cp gene and to determine its identity and copy number. The Southern analysis showed three bands of different size, i.e. 1.1 kb, 1.3 kb, and 5.8 kb. Most of the lines of T5 generation have one insertion site, suggesting that the three insertion sites were located in different loci. Based on the phenotypic data, the transgenes of 1.1 kb and 1.3 kb were functional, resulting in resistant or recovery phenotype, while that of 5.8 kb was not functional. Copy number apparently had no effects on the phenotypes.

FORMULA BERBAHAN AKTIF PSEUDOMONAD FLUORESEN DAN PENGARUHNYA TERHADAP PERKEMBANGAN PENYAKIT LAYU PADA CABAI Yenny Wuryandari; Sri Wiyatiningsih; Maroeto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Formula with active ingridient of fluorescent pseudomonads and its influence on wilt disease development of pepper. The purpose of this study was to synergize organic fertilizer with biopesticides of fluorescent pseudomonads 122 to be the best formula to increase the resistance of pepper plants to wilt disease. The study used a completely randomized design (CRD), which consisted of four treatments. The treatments were forms of active ingredient formula of fluorescent pseudomonads (powder, pellet, granule, and liquid). The effectiveness of the formula in suppressing development of the disease was measured by observing the incubation period, index of the disease and discoloration of vascular tissue of pepper. The results showed that the disease incubation period of plant treated with liquid and powder formula was longer than formula with granule and pellet. Plants treated with powder formula showed the lowest disease index compared to control, liquid, pellet and granule formula. Disease index and discoloration in vascular tissue was also of the lowest value when the plants were treated with powder formula. This result indicate that active ingredient of fluorescent pseudomonads formulated in powder was the most effective to inhibit the development of wilt disease on pepper caused by Fusarium sp. and Ralstonia solanacearum.

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