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JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

JHPT TROPIKA
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Published by Universitas Lampung

ISSN : 14117525 EISSN : 24610399 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.

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Articles 521 Documents

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VARIAN SOMAKLONAL KACANG TANAH RESISTEN SCLEROTIUM ROLFSII HASIL SELEKSI IN VITRO MENGGUNAKAN FILTRAT KULTUR CENDAWAN Yusnita, Hajrial Aswidinnoor, Rusmilah Suseno & Sudarsono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Peanut somaclonal variants resistant to Sclerotium rolfsii derived from in vitro selection with fungal culture filtrates. Sclerotium stem rot is one of the most important peanut disease which often caused significant yield loss. The use of peanut cultivars resistant to Sclerotium rolfsii infection is the most efficient way to control the disease. Attempts to obtain peanut lines with tolerance or resistance to Sclerotium stem rot through induction of somaclonal variation and in vitro selection using fungal culture filtrates (CF) have been conducted previously. Somatic embryo (SE) clumps that had been maitained in culture for approximately one year in regeneration medium were exposed onto selective medium containing 30% S. rolfsii CF for three consecutive 1 month –passages, and the SE formed after selection periods were considered to be insensitive to the fungal CF. A number R0 peanut lines have been regenerated from CF-insensitive SE, and their R1 and R2 progenies were grown in a plastic house for evaluation on qualitative and quantitative variant characters. Early identification for tolerance to S. rolfsii was also conducted among R0 peanut lines, and the results have shown enhanced resistance when compared to the original non-selected cultivar. However, further evaluation is needed to study responses of the R1 and R2 progenies of the somaclones against S. rolfsii infection. The objective of this particular study was to evaluate responses of R1 and R2 peanut somaclones derived from fungal CF-insensitif SE against S. rolfsii infection in the plastic house. Results of this experiment showed, a number of resistant somaclonal variants were obtained among R1 and R2 population, which segregated as resistance and susceptible to S. rolfsii infection. The resistance somaclonal variants initially showed stem rot symptoms after inoculation with S. rolfsii with disease score (DS) of 1 to 3. However, as the plants grew and developed, they showed wound-healing process at the lesio and they were able to produce the same or higher number of filled pod as the original non-inoculated peanut plants.

POTENSI TRIGONA SPP. SEBAGAI AGEN PENYEBAR BAKTERI RALSTONIA SOLANACEARUM PHYLOTIPE IV PENYEBAB PENYAKIT DARAH PADA TANAMAN PISANG . Mairawita; Trimurti Habazar; Ahsol Hasyim; Nasril Nasir
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Banana blood disease (Blood Disease Bacteria, BDB) caused by Ralstonia solanacearum Phylotype IV is the most important banana disease in Indonesia. So far, information on the spread of disease by insects is very limited. The research was aimed to determine the role of insect as a disseminator of R. solanacearum Phylotype IV and to determine the amount of BDB inoculum carried by each individual insect. The experiment was conducted in May - September 2008. Samples of insects (adult insects, the young insects, larvae, eggs), nectar, and pollen were taken from a colony of Trigona spp. collected from BDB endemic area, Baso plateau (876 m asl) using purposive sampling method. Active adult insects were collected from the BDB infected banana flowers and healthy banana flowers. BDB on adult insects was isolated from the caput and abdomen, while for the young insects, larvae and pupae the isolation source were not differentiated. Each of the samples was rinsed, macerated, and cultured on medium containing Triphenyl Tetrazolium Chloride (TTC). BDB isolate characterization and identification were conducted through morphological, physiological, and pathogenicity tests. The parameter observed was the: population of BDB (cfu/ml) on each stage of insect development. The results showed that BDB can be isolated from the outside and the inside of the body of an adult, a young insect, pupa, larva also on pollen and nectar but it was not found in eggs. BDB population was higher in inside part fo the insect body in each phase of the development of the insect. From all phases, the BDB was higher in inner part of the body of adult insects which have visited infected banana flower.

PENYAKIT HAWAR DAUN PINUS MERKUSII DI BERBAGAI PERSEMAIAN KAWASAN UTAMA HUTAN PINUS JAWA TIMUR Sutarman .; Andriani Eko Prihatiningrum
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Leaf blight disease of Pinus merkusii in various nurseries of pine forest in major area of East Java. This study aims to: (i) obtain isolates of pathogens, (ii) determine disease on seedling growth during the critical period and the young plants, (iii) determine the effect of pathogenic isolates on disease severity, and (iv) determine the effect of altitude on severity of seedlingâ€™s leaf blight disease of Pinus merkusii from various nursery locations in East Java. The experiment was conducted in February-August 2014 at nine locations in the nursery and one young pine plantation site. Disease index data were analyzed using Microsoft Office Excel 2007 software to determine the regression model of the relationship between the index of disease with the independent variables and with Anova followed by Duncan test to find out the effect of altitude difference to the index of disease. Pathogen that causes late blight is Pestalotia theae Sawada. In the critical period, seedling disease index increased from 7.38 to an average of 26.96 in the nursery RPH Celaket; while on the young plants in the field in RPH Kemiri disease index ranged 28-31. Differences in altitude did not affect disease index difference. Based on disease index, three isolates of pathogens that require serious attention are Wagir isolates (985 m asl., the most virulent), Ngantang (500 m asl.), and South Pujon (1200 m asl.).

KETAHANAN BEBERAPA KULTIVAR CABAI TERHADAP CUCUMBER MOSAIC VIRUS DAN CHILLI VEINAL MOTTLE VIRUS Muhammad Taufik, Sri Hendrastuti Hidayat Sriani Sujiprihati, Gede Suastika dan Sientje Mandang Sumar
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 2 (2007): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Resistance Evaluation of Chillipepper Cultivars for Cucumber Mosaic Virus and Chilli Veinal Mottle Virus. The use of resistance culivars is an important strategy for management of virus infection in chillipepper. A research was undergone to study the effect of single and mix infection of CMV and ChiVMV on the disease incidence and on the growth and yield of nine chillipepper cultivars, i.e. Cilibangi 4, Cilibangi 5, Cilibangi 6, Helem, Jatilaba, Tit Bulat, Tit Segitiga, Tit Super and Tampar. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA. In general, inoculated chillipepper cultivars developed similar symptoms, i.e. mosaic type for CMV and mottle type for ChiVMV. More severe symptom was not always observed from mix infection of CMV and ChiVMV. Disease incidence occurred in the range of 16.67 – 86.0% and this caused 18.3 – 98.6% yield loss. Based on symptom expression, ELISA result, and reduction on yield, it can be concluded that all chillipepper cultivars used in this study could not hold up the virus infection. However, several cultivars showed tolerance response : Jatilaba, Tit Super, and Tampar for CMV; Cilibangi 4 for ChiVMV; Tit Super for mix infection; and Cilibangi 5 for CMV, ChiVMV, and mix infection. Further evaluation and investigation involving different chillipepper cultivars should be conducted.

SERANGAN, KEPADATAN POPULASI, DAN KEANEKARAGAMAN SERANGGA PADA PERTANAMAN JAGUNG YANG DIKELOLA DENGAN OLAH TANAH KONSERVASI VERSUS OLAH TANAH KONVENSIONAL DI NATAR, LAMPUNG SELATAN F.X. Susilo dan I Gede Swibawa .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 2 (2001): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Infestation, population, and diversity of insects in corn fields managed under conservation versus full tillage in Natar, South Lampung. This experimental study that has been conducted during the rainy season of 2000 was aimed to document the attacks, population density and diversity of selected insects in corn fields managed under conservation tillage (sprayed with Roundup herbicide) versus full tillage systems (plowed, no herbicide). Three corn cultivars were planted in four 80 x 60 m plots (larger plots) differing in tillage systems (C7-CT= cultivar C7 grown under conservation tillage system; RRCorn-CT = RRCorn under conservation tillage; C7-FT = C7 under full tillage; Bisma-FT = Bisma under full tillage). All corn seeds were treated with imidacloprid insecticide except those sowed in a control plot (10 x 10 m) that was set up inside the larger plot. Variables being observed were (1) attacks of corn whorl maggots or other organisms, (2) attacks of leaf feeders and cornearworms and (3) diversity of surface active insects and population density of ants + mites. Results show that, first, seed treatment using imidacloprid insecticide could reduce corn whorl maggot attacks only in Bisma-FT. Second, leaf feeder attacks were varied across tillage systems but did not exceed 4% level. Meanwhile, despite apparently high attack of the cornearworm in the field, no significant injury was detected either in the harvested ears or seeds. Third, pitfalls caught at least 66 families of surface-active insects consisting mostly of ants (65%), springtails (12%), mites (8%) and spiders (5%). Tillage systems did not seem to affect the diversity of the surface-active insects. Compared with the other three plots, the RRCorn-CT plot was dwelled by more ants and mites. Ants and mites were both accounted for more seedling failure in the RRCorn-CT plot while corn whorl maggots were responsible for the seedling failure in the Bisma-FT plot.

Kemampuan Predasi Tungau Predator Amblyseius sp. Resisten Temperatur terhadap Tetranychus urticae Budianto Heru Bambang; Edi Basuki
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

he predation ability of temperature resistant Amblyseius sp. to Tetranychus urticae. Global warming and climate changes have caused great mortality of the predatory mites Amblyseius sp. that disrupts the natural control of Tetranychus urticae. In contrast, the low humidity and high temperature led to an increase in the population of T. urticae. The purposes of this study were to determine the effect of temperature on population of the predatory mites Amblyseius sp. and to determine the predation ability of temperature resistant Amblyseius sp. on T. urticae. The selection of Amblyseius sp. was conducted at temperatures of 15, 20, 25, 30 and 35 0C. The parental predatory mite Amblyseius sp., and subsequent descendants were exposed to the temperature range until the values of the LT50 fiducial limits (FL) of a certain generation did not overlap with that of the previous one. The effectiveness of Amblyseius sp. predation ability was studied using an experimental method i.e. completely randomized design with four treatments and ten replications . For this purpose, each stage of T. urticae, i.e. egg, larvae, nymph and adult was set as treatment and was given to any type of temperature resistant predatory mite Amblyseius sp. The results showed that by using the temperature gradient, we managed to select temperature-resistant individuals from their population. These individuals formed a population that was resistant to temperature of up to 33.3oC without losing their predatory capacity, especially on the egg stage of T.urticae.

JAMUR ENTOMOPATOGEN ASAL TANAH LEBAK DI SUMATERA SELATAN DAN POTENSINYA SEBAGAI AGENSIA HAYATI WALANG SANGIT (LEPTOCORISA ORATORIUS (F.)) Effendy TA, Robby Septiadi, Abdullah Salim & Abdul Mazid .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 2 (2010): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of Stink Bug (Leptocorisa oratorius (F.)). The purpose of this research was to explore and examine the potential of soil borne entomopathogenic fungi from South Sumatera as biological agents of Leptocorisa oratorius (F). Soil used for trapping entomopatogenic fungi, was taken from five locations in South Sumatera. Percentage of nymph mortality was analyzed by using analysis of variance and arranged in Completely Randomized Design. LT50 of nymph mortality was determined with Probit Analysis. Entomopathogenic fungi found in this research were Beauveria bassiana and Metarhizium sp. Stink bug mortality due to B. bassiana isolates reached 40-73.3% and Metarhizium sp. reached 56.7-70%. The results showed that Metarhizium isolates were more virulent than B. bassiana isolates. The viability of B. bassiana conidia did not decrease during sub-culturing. Sub-culturing did not influence the mortality of stink bug nymph but it prolonged the LT50 of the stink bug.

PENEKANAN PERKEMBANGAN PENYAKIT BUSUK BATANG VANILI (FUSARIUM OXYSPORUM F.SP. VANILLAE) MELALUI SELEKSI ASAM FUSARAT SECARA IN VITRO Endang Nurcahyani; Issirep Sumardi; Bambang Hadisutrisno; E. Suharyanto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The most biological constrain on Vanilla planifolia plantation recently was caused by epidemical disease that laterdecrease vanilla production. The most important disease on vanilla is foot rot disease caused by Fusarium oxysporum f. sp.vanillae. So far, the disease has not been successfully controlled although some experiments had been conducted. Onealternative method has been introduced by using a new cultivar which was resistance to Fusarium). A mutant vanilla to thefungus has been initiated by in vitro selection on medium containing fusaric acid. The aims of this research were: (1) to investigate effective concentration of fusaric acid used for in vitro selection, (2) to characterize mutants which have been set up and also to test those mutants for their resistance to the fungus. The results showed that: (1) fusaric acid at the concentration of 110 ppm effectively suppressed the disease intensity up to 25% compared to the concentration of 90 ppm and 100 ppm. In other words, 110 ppm of fusaric acid has increased the category criterion from moderate to resistant, (2) there was an increase of the total phenol content and thickness of lignin in vanilla stem, and (3) the protein profile of vanilla plantlet was different from the control. There was an initiation of a new band of about 18 kD in a mutant predicted as a protein which is responsible for vanilla resistance to Fusarium.

KERAGAMAN DAN KEMELIMPAHAN COLLEMBOLA SERTA ARTHROPODA TANAH DI LAHAN SAWAH ORGANIK DAN KONVENSIONAL PADA MASA BERA Indriyati dan Lestari Wibowo .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 8 No. 2 (2008): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The diversity and abundance of Collembola and soil arthropods in organic and conventional lowland field at fallow time. As ecosystem components, Collembola and arthropods are important in keeping the ecosystem stability. Beside its role as the decomposer, Collembola also takes part as the alternative food for arthropod predators. This research was aimed to gather information on the diversity and abundance of Collembola and arthropods in organic and conventional lowland field at fallow time. The sampling was done using pitfall trap and Berlese funnel. The results showed that: (1) the abundance of Collembola in organic lowland field was higher than that of in the conventional lowland field but the diversity of Collembola in the organic lowland field was not different from that of in the conventional field; (2) Entomobryidae was the family with the highest abundance; and (3) the diversity and abundance of soil arthropods were relatively greater in organic lowland field than that of in the conventional lowland.

PERKECAMBAHAN UREDOSPORA HEMILEIA VASTATRIX PADA EKSTRAK RIMPANG JAHE DAN KUNYIT SERTA DAUN CENGKEH DAN SIRIH Cipta Ginting .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 6 No. 1 (2006): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Germination of Hemileia vastatrix uredospores on crude water extracts of zinger and turmeric rhizome and clove and Piper betle leaves. Coffee leaf rust caused by H. vastatrix especially on Arabica coffee is one of most important diseases in coffee and conventional control methods of leaf coffee rust is still unsatisfactory. The objective of this study was to determine the effect of crude water extract on the germination of H. vastatrix uredospores. The study was conducted from January to September 2004 in the Laboratory of Plant Pathology at Unila. In each test, treatments were arranged in a randomized complete block design with four replications. Crude water extract was prepared by homogenizing 100 g of material in 100 ml sterilized distilled water. After being passed through four layers of sterilized cheesecloth, the mixture was defined as the aliquot (100% extract) and dilutions were made with sterile distilled water to obtain concentrations of 2.5 to 10%. Each of four materials (zinger, turmeric, clove, and Piper betle) was tested separately in five aliquot concentration levels. One ml of each extract was mixed with 0.25 ml of uredospora suspension (4 x 105 per ml), and 0.2 ml of the mixture was incubated. The variable was germinated uredospora (%) that was determined under a mikroscope. The results show that significant reduction in spore germination occurred by turmeric, clove, and P. betle extracts at > 2.5% and by zinger extract at > 5%. Some uredospora exposed to plant extract germinated abnormally: germ tubes shorten, swollen, or malform.

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