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JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

JHPT TROPIKA
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Published by Universitas Lampung

ISSN : 14117525 EISSN : 24610399 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.

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Articles 521 Documents

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POTENTIAL IMPACT OF METARHIZIUM ANISOPLIAE ON THE DIAMONDBACK MOTH (LEPIDOPTERA: PLUTELLIDAE) AND ITS PARASITOID DIADEGMA SEMICLAUSUM (HYMENOPTERA: ICHNEUMONIDAE) Rosma Hasibuan, Nilly Christalia, F.X. Susilo1, and Nur Yasin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 9 No. 2 (2009): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Potential Impact of Metarhizium anisopliae on the Diamondback Moth (Lepidoptera: Plutellidae) and Its Parasitoid Diadegma semiclausum (Hymenoptera: Ichneumonidae). Laboratory studies were conducted to evaluate the effect of the Metarhizium anisopliae against the diamondback moth, Plutella xylostella and its parasitoid, Diadegma semiclausum. A completely randomized design consisted of 5 treatments (4 concentrations of conidial suspension: 5 x 104, 3.5 x 105, 2.5 x 106, 1.2 x 107 conidia/ml and control) was used. The results indicated that the mortality of P. xylostella larvae were significantly induced by the fungal treatments. A significant reduction in pupation and adult emergence of P. xylostella was also detected in all treatments when compared with that in the control. The fungus might also result in a male-biased sex ratio of the surviving P. xylostella. When applied at a concentration of 1.2 x 107 conidia/ml, M. anisopliae might significantly reduce the survival of the parasitoid, D. semiclausum. Thus, despite its potential as a biological control agent against P. xylostella, the entomomogenous fungus M. anisopliae was also detrimental to the larvae parasitoid D. semiclausum.

KERAGAMAN ARTHROPODA PADA PADI SAWAH DENGAN PENGELOLAAN TANAMAN TERPADU I Nyoman Widiarta, Dede Kusdiaman, dan Suprihanto1 .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 6 No. 2 (2006): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Arthropods diversity in rice field with integrated crop management. Integrated crop management (ICM) has been introduced to improve rice intensification system in Indonesia. Organic matter has been recommended to be added into the rice fields to improve soil fertility. In order to minimize hazard of pesticide integrated pest management (IPM) is incorporated into ICM to control pest. The objectives of this research were to study arthropod diversity in rice field where organic matter was added and pesticide was used rationally. The research was conducted at experimental fields of Indonesian Center for Rice Research in Sukamandi, during the wet season of 2005/2006. Diversity of arthropod was observed in ICM, organically-farm field, and farmer technique paddy fields. Arthropod was sampled using a FARMCOP suction apparatus. Results of this study indicated that in ICM paddy fields were harbored by more insect, predators and parasitoids especially during early growing stage of rice plants. Thus adding organic matter and rationaly use of insecticide could increase the natural enemies for insect pests in the field.

KARAKTER MOLEKULER CHRYSANTHEMUM B CARLAVIRUS (CVB) ISOLAT KRISAN (DENDRANTHEMA GRANDIFLORA KITAM) DI INDONESIA I G. R. M. Temaja, G. Suastika, S.H. Hidayat dan U. Kartosuwondo .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 8 No. 2 (2008): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Molecular characterstics of Chrysanthemum B Carlavirus (CVB) isolated from chrysanthemum (Dendranthema grandiflora Kitam) in Indonesia. Chrysanthemum B Carlavirus (CVB) belongs to Carlavirus genus which type species is Carnation latent virus (CLV). Since CVB is considered a new plant virus in chrysanthemum plantation in Indonesia, a study on its molecular characters is required. The objectives of the study are: 1) to determine molecular characters of CVB; 2) to study genetic diversity among CVB isolates collected from different geographic regions in Indonesia. The research activities cover virus purifications, electron microscope observation, coat protein analysis by SDS PAGE, and nucleic acid analysis. The result of virus purification demonstrated a high purity level with ratio value of A260/A280 =1.22. The total pure virus produced from 200 g of fresh material is 6.250 mg. Purified virus preparation yielded rather straight rod and flexuous virus particles of about 685 nm long and 12 nm wide. Coat protein analysis with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed specific protein band of approximately 34 kDa. Specific DNA fragment of 739 bp was successfully amplified from chrysanthemum infected by CVB Cianjur, Medan, Malang and Bali isolates. CVB isolated from Cianjur, Medan, Malang and Bali have similarity 85-99%. Based on analysis using PAUP 4.10 program, Cianjur, Medan, Malang and Bali isolates belong to the same group with CVB isolates originated from India (Chattisgarh and Jammu isolates). Cianjur isolate has close relationship to Medan isolate, however Bali isolate showed a close relationship with Malang isolate.

POTENSI BAKTERI ENDOFIT AKAR UBI JALAR (IPOMOEA BATATAS L.) ASAL KABUPATEN SORONG PAPUA BARAT SEBAGAI AGENSIA BIOKONTROL MELOIDOGYNE SPP. ., Tuminem; Sinaga, Meity S; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 15, No 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Potency of sweetpotato (Ipomoea batatas L.) root endophytic bacteria from Sorong District West Papua as biocontrol agent of Meloidogyne spp. Root knot nematodes/RKN, Meloidogyne spp. is one of the important pathogens in sweet potato plant. The disease incidence rate by the RKN on sweetpotato crop in Sorong District reached 88.77%. This study aims to get the sweet potato root endophytic bacteria that have potential as biocontrol agents against Meloidogyne spp. Endophytic bacteria was isolated from the roots of healthy sweet potato sampled from Sorong District, West Papua Province. Isolation and selection of bacteria using TSA media. Selected bacterial isolates, which were non-pathogenic to plants and humans then were identified with PCR technique using universal primer 63-F / 1387-R. The ability of bacteria to produce the lipase enzyme was selected using the media NB agar and rhodamine B. The protease enzyme-producing bacteria were selected using skim milk media. The chitinase enzyme-producing bacteria were selected using the colloidal chitin media. Production of cyanide was detected using filter paper soaked in a solution of CDS. The effectiveness of culture filtrate of bacteria as biocontrol agents was measured based on the percentage of 2nd juvenile mortality and egg hatching of Meloidogyne spp. Four isolates of endophytic bacteria, that were Enterobacter sp EAS (1a), Enterobacter sp. EAS (3a) Enterobacter ludwigii EAS (4), and Burkholderia cepacia EAS (6) produced lipase and protease. In addition, B. cepacia EAS (6) also produced chitinase. Those isolates caused mortality of the 2nd juvenile 81.4 to 95.2% and inhibited the egg hatching of Meloidogyne spp. 53.13 to 81.92%.

AKTIVITAS EKSTRAK BIJI TANAMAN MINDI MELIA AZEDARACH (L.) TERHADAP SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE) Hamdani .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 1 (2005): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Activity of Melia azedarach (L.) seed extract against armyworm Spodoptera litura (F.) (Lepidoptera: Noctuidae). This study was conducted to evaluate the effectivenes and biological activity of Melia azedarach L. (Meliaceae) seed extract against armyworm, Spodoptera litura F. The first instar larvae were fed extract-treated cotton leaves for 2 days, then were maintained on untreated leaves until the third instar stage. Records were kept in regard to the larvae mortality and developmental time of surviving larvae from first instar to third instar. The result showed that Melia azedarach L. seed extract at consentration of 50 g of seeds/l of water (5%) exhibited moderate insecticidal activity against S. litura larvae (43.33 - 68.33% mortality). Addition of detergen at 0.2% to extract did not increase insecticidal activity of the extract. However, boiling seed extract at consentration of 50 g of seeds/l of water (5%) during 10 until 20 minutes increased insecticidal activity of extract (66.67 - 68.33% mortality). Generally, M. azedarach seed extract treatment did not affect developmental time of S. litura larvae.

VIRULENSI EMPAT ISOLAT CHILLI VEINAL MOTTLE POTYVIRUS PADA TANAMAN CABAI (CAPSICUM ANNUUM L.) Ifa Manzila; Sri Hendrastuti Hidayat; Ika Mariska; Sriani Sujiprihati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Infection of Chilli veinal mottle potyvirus (ChiVMV) has been reported from chilli pepper growing area in Indonesia. Field observation showed wide variation of symptom development starting from mild mottle to severe mottle and vein banding. A research was conducted to study the difference of isolates of ChiVMV collected from West Java (Cikabayan), Central Java (Karadenan), South Kalimantan (Nusa Indah), and West Sumatera (Tanah Datar) based on their response on 10 genotypes of chilli peppers i.e. Jatilaba, Helem, VC246, Keriting Bogor, PBC485, Titisuper, Beauty Bell, Gelora, IPBC Tanjung, and Keriting Sumatera. Each isolates of ChiVMV was mechanically inoculated to each chilli pepper genotype, and observation was conducted every day to record incubation period, symptoms type, and disease incidence. Cikabayan and Nusa Indah isolates were able to infect all chilli pepper genotypes with the shortest incubation period of 3 days in “Tistsuper” and “Beauty Bell” genotypes; whereas Karadenan and Tanah Datar isolates only infected 4 and 6 chilli pepper genotypes, respectively. Although the 4 isolates of ChiVMV showed differences in their host range, but most of them developed similar symptoms ranging from mottle, vein banding, leaf cupping, and malformation. Based on the host response, ChiVMV isolates from Cikabayan and Nusa Indah were considered more virulent than the isolates from Karadenan and Tanah Datar.

Pengimbasan Ketahanan Tanaman Pisang Terhadap Penyakit Darah (Ralstonia solanacearum Phylotipe IV) MengGunakan Bakteri Endofit Husda Marwan .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Induced resistance of banana against blood disease (Ralstonia solanacearum Phylotipe IV) using endophytic bacteria. Endophytic bacteria play a role to control plant pathogenic bacteria indirectly by inducing the plant to increase production of the metabolites in activating the plant resistance. This study aimed to determine the ability of endophytic bacteria to induce resistance in banana plants against blood disease. Indicators of induced resistance of banana plant were analyzed through the existence of defense enzyme activities (peroxidase and polyphenol oxidase) and the amount of salicylic acid on banana roots. The results showed that endophytic bacteria isolates EAL15, EKK10, EKK20 and EKK22 suppressed the incidence of blood disease in Cavendish banana by 70-85%. Increased activity of peroxidase and polyphenol oxidase in roots of banana plants were higher in plants treated with endophytic bacteria compared to untreated plants. Salicylic acid content in banana plants treated with isolates EAL15, EKK10 and EKK20 was higher than that of EKK22 isolates and control plants.

PENGARUH INFEKSI TMV (TOBACCO MOSAIC VIRUS) TERHADAP PERTUMBUHAN VEGETATIF DAN GENERATIF BEBERAPA VARIETAS CABAI MERAH (CAPSICUM ANNUUM L.) Hasriadi Mat Akin dan Muhammad Nurdin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 3 No. 1 (2003): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Influence of tobacco mosaic virus infection to vegetative and generative growth of various hot pepper varieties (Capsicum annuum L.). This experiment was conducted to investigate the effect of TMV infection on vegetative and generative growth of various hot pepper varieties. Treatments were arranged in completely randomized design in split plot experiment with four replications. Mainplots were virus-inoculated and uninoculated plants; subplots were three hot pepper varieties: Cimerti, HP-Typhoon, and HP-Tornado. The results of the experiment showed that TMV infection caused decrease vegetative and generative growth. The decrease of vegetative and generative growth indicated by the reduction of leaf width, plant height, and yield. HP-Tornado and Cimerti varieties showed susceptible reaction proved by significant reduction of the growth and yield; HP-Typhoon was tolerance reaction to TMV infection indicated by significant reduction of the growth and lowest reduction of the yield.

KETERTARIKAN WALANG SANGIT (Leptocorisa oratorius F.) TERHADAP BEBERAPA BAHAN ORGANIK YANG MEMBUSUK Solikhin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 1 (2001): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Attractiveness of Rice Bug (Leptocorisa oratorius F.) to Several Faunal Decaying Materials. An experiment was conducted in Yogyakarta from October 1996 to September 1997 to know (1) the attractiveness of adult male versus female rice stink bug in to decaying crab, (2) the attractiveness of the rice stink bug from the result of point-1 to five decaying materials, and (3) the volatile compounds emitted by each decaying material. For the first objective, 50 rice stink bugs (sex ratio 1:1) were released into a preference test cage for one hour. Data was then analyzed with X2 at 1 and 5%. For the second objective, five replicates of five different treatments were arranged in randomized completely block design. As many as 125 rice stink bugs were released into the central part of the cage consisting of five equal parts of attractants for one hour. Data of rice stink bug from each part was analyzed with Anova continued with Duncan's Test at 1 and 5%.. Volatile compounds emitted by each material were captured based on the method of Gamliel and Stapleton (1993) then identified with gas chromatography mass spectrophotometry (GC-MS) and (or) capillary gas chromatography. The results were that: (1) adult male rice bug was significantly more attracted to that of female in response to sixth-day-decaying-crab, (2) adult male rice bug was most attracted to sixth-day-decaying cow blood compared to four others, and (3) volatile compounds emitted by those five materials were carbon dioxide, methanol, ethanol, aceton, dimethyl sulphide, dimethyl disulphide, ammonia, acetic acid, and an unidentified compound.

Identifikasi Penyebab Penyakit Daun Keriting Kuning pada Tanaman Mentimun Dwiwiyati Nurul Septariani; Sri Hendrastuti Hidayat; Endang Nurhayati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

ABSTRACTIdentification of the causal agent of yellow leaf curl disease on cucumbers. Yellow leaf curl disease has been reported to cause serious diseases and yield losses on tobacco, chilli pepper, and tomato plants in Java. Similar symptoms were observed recently on cucumber plants from several growing areas in West Java (Bogor), Central Java (Tegal and Sukoharjo), and Yogyakarta (Sleman). Symptom variations including mosaic, chlorotic spotting, leaf curling, blistering, vein banding, reduction and distortion of leaf and fruit were observed. Serological detection using Enzyme Linked Immunosorbent Assay (ELISA) showed infection of several viruses. Antibodies specific to Squash mosaic comovirus (SqMV), Zucchini yellow mosaic potyvirus (ZyMV), dan Cucumber mosaic cucumovirus (CMV) were reacted positively with field samples. No serological reactions were observed with antibodies to Tobacco ringspot potyvirus (TRSV) and Watermelon mosaic potyvirus (WMV). Molecular detection approach based on Polymerase Chain Reaction was undergone using universal primers for Geminivirus, pAL1v1978 and pAR1c715. DNA fragment 1600 bp in size, was successfully amplified from leaf samples originated from Tegal, Sleman, Bogor, and Sukoharjo. Further identification by nucleotide sequencing indicated that virus isolates causing yellow leaf curl disease on cucumber have highest homology (95.7% to 98.6%) with Tomato leaf curl New Delhi virus-[Cucumber:Indonesia] (AB613825) from Klaten, Central Java, Indonesia.

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