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Ilsan, Noor Andryan
Department Of Medical Laboratory Technology, STIKes Mitra Keluarga, Margahayu, Bekasi, Indonesia
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IDENTIFIKASI JAMUR PADA LALAT DI YAYASAN TUNAS MULIA BANTAR GEBANG Ika Nurfitrianti; Maulin Inggraini; Noor Andryan Ilsan
Jurnal Mitra Kesehatan Vol. 2 No. 1 (2019): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v2i1.22

Abstract

Pendahuluan: Yayasan Tunas Mulia, Bantar Gerbang merupakan sekolah alam yang terdapat di daerah Bantar Gebang. Siswa Yayasan Tunas Mulia tinggal didaerah TPST Bantar Gebang sehingga banyak ditemukan lalat di sekitar tempat tinggal mereka. Kelompok lalat yang sering kita temui di lingkungan yaitu lalat rumah, lalat hijau dan lalat daging. Lalat dapat menularkan berbagai macam penyakit terutama penyakit yang disebabkan oleh jamur. Tujuan penelitan ini adalah untuk mengidentifikasi jamur pada lalat di Yayasan Tunas Mulia Bantar Gebang. Metode: Metode penelitian ini secara Cross Sectional yaitu mencuplikan seekor sampel lalat dalam satu waktu. Objek penelitian adalah Lalat yang ditangkap di Yayasan Tunas Mulia Bantar Gebang. Hasil: Hasil yang diperoleh dari penelitian ini adalah didapatkan dua jenis spesies lalat yaitu Musca domestica dan Chrysomya megachepala. Jamur yang di dapatkan pada permukaan tubuh lalat Musca domestica yaitu Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, dan pada permukaan tubuh lalat Chrysomya megachepala yaitu Aspergillus fumigatus dan Aspergillus flavus Kesimpulan: Perlu dilakukan penelitian lanjutan untuk mengetahui penyakit yang disebabkan oleh jamur dari penyebaran lalat dilingkungan masyarakat.
UJI SENSITIVITAS ANTIBIOTIK KOTRIMOKSAZOL TERHADAP BAKTERI Salmonella sp. DENGAN METODE MODIFIKASI KIRBY-BAUER Dinar Ikhwal Prasetia; Maulin Inggriani; Noor Andryan Ilsan
Jurnal Mitra Kesehatan Vol. 2 No. 1 (2019): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v2i1.23

Abstract

Pendahuluan: Salmonellosis adalah penyakit infeksi pada manusia dan hewan yang disebabkan oleh bakteri Salmonella sp.. Terapi utama yang dipakai dalam penanganan Salmonellosis adalah dengan pemberian antibiotik. Setiap daerah mempunyai pola sensitivitas Salmonella sp. yang berbeda. Sensitivitas antibiotik terhadap suatu bakteri sangat penting untuk menyesuaikan pengobatan terbaru dan melihat manfaat dari pengobatan sebelumnya. Tujuan dari penelitian ini adalah mengetahui sensitivitas bakteri Salmonella sp. terhadap antibiotik kotrimoksazol. Metode: Penelitian dilakukan di Laboratorium Mikrobiologi Sekolah Tinggi Ilmu Kesehatan Mitra Keluarga Bekasi Timur pada bulan September 2017. Penelitian ini merupakan penelitian deskripstif dengan pendekatan rancangan studi cross sectional. Teknik pengambilan sampel pada penelitian ini adalah purposive sampling. Uji sensitivitas antibiotik dilakukan dengan menggunakan metode difusi cakram kertas modifikasi Kirby-Bauer. Prinsip dari metode ini adalah penghambatan terhadap pertumbuhan mikroorganisme, yaitu zona hambatan akan terlihat sebagai zona jernih disekitar daerah yang mengandung zat antibakteri kotrimoksazol. Media yang digunakan pada penelitian ini adalah Mueller Hinton Agar (MHA) dengan menggunakan standar kekeruhan MC Farland 0,5. Penilaian sensitivitas antibiotik kotrimoksazol berdasarkan ukuran zona hambatnya dengan mengukur besarnya diameter daya hambat yang terbentuk disekitar cakram kertas antibiotik tersebut. Diameter zona hambat yang terbentuk semakin besar, maka semakin besar pula sensitivitas antibiotiknya. Interpretasi hasil didasarkan pada zona hambat yang terbentuk dan disesuaikan dengan kriteria standar dari Clinical and Laboratory Standards Institute (CLSI) 2014. Jumlah sampel dalam penelitian ini sebanyak 9 sampel isolat Salmonella sp. yang berasal feses. Hasil: Hasil uji sensitivitas antibitotik kotrimoksazol terhadap bakteri Salmonella sp. menunjukkan bahwa bakteri Salmonella sp., sensitif 100% terhadap antibiotik kotrimoksol dengan rerata diameter zona hambat 31,11 mm. Kesimpulan: Berdasarkan uraian kesimpulan di atas dapat dilakukan penelitian rutin untuk periode tertentu mengenai pola sensitivitas dari bakteri penyebab demam tifoid di berbagai daerah secara berkala karena masing-masing daerah mempunyai pola sensitivitas yang berbeda dan bervariasi pada waktu dan tempat yang berbeda.
BAKTERI PATOGEN DALAM SPONS CUCI PIRING PADA PENJUAL MAKANAN DI PASAR MARGAHAYU, BEKASI TIMUR Yola Violita Agustin; Noor Andryan Ilsan; Maulin Inggraini
Jurnal Mitra Kesehatan Vol. 2 No. 1 (2019): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v2i1.24

Abstract

Pendahuluan: Makanan dan minuman yang tidak memenuhi persyaratan kesehatan jika dikonsumsi  akan menimbulkan gangguan kesehatan seperti diare, kolera, disentri, demam tifoid dan keracunan makanan. Menurut data Kemenkes tahun 2017 kasus diare pada tahun 2016 dengan Case Fatality Rate (CFR) mencapai 3.04% dengan 6 orang meninggal dari 198 kasus. Kebersihan peralatan makan merupakan salah satu aspek dalam hygiene dan sanitasi makanan. Proses pencucian peralatan makan yang benar akan berdampak pada hygiene dan sanitasi yang baik. Spons cuci piring umumnya digunakan untuk menghilangkan sisa makanan. Sisa makanan yang terdapat pada spons akan mendukung lingkungan bakteri untuk tumbuh. Spons yang terkontaminasi dapat mengkontaminasi peralatan makan, sehingga menyebabkan penularan penyakit bawaan makanan. Studi kasus di Amerika Serikat menunjukkan bahwa terjadi hampir 38.6 juta kasus penyakit akibat penyebaran penyakit bawaan makanan. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri patogen serta jenis bakteri patogen yang terdapat pada spons cuci piring pada penjual makanan. Metode: Identifikasi bakteri patogen dilakukan pada 10 spons cuci piring yang digunakan penjual makanan di Pasar Margahayu. Identifikasi bakteri menggunakan pewarnaan Gram dan uji biokimia. Hasil: Jenis bakteri patogen yang teridentifikasi adalah Escherichia coli, Staphylococcus aureus, Pseudomonas aeroginosa, Enterobacter aerogenes, dan Proteus sp. Persentase isolat yang ditemukan adalah 80% spons mengandung S. aureus, 70% mengandung E. aerogenes, 20% mengandung E.coli, 20% mengandung P.aeroginosa, dan 10% mengandung Proteus sp. Kesimpulan: sampel spons cuci piring yang telah dilakukan pewarnaan Gram dan uji biokimia menunjukkan kecurigaan terhadap koloni berwarna putih transparan adalah Proteus sp. koloni putih transparan bulat kecil adalah Enterobacter aerogenes, putih bulat besar adalah Escherichia coli, putih bulat kecil adalah Pseudomonas aeroginosa, koloni merah pada media SSA adalah Enterobacter aerogenes, dan koloni putih dengan zona kuning adalah Staphylococcus aureus.
PENENTUAN KADAR ALKOHOL PADA PEMINUM ALKOHOL DENGAN METODE ALCOHOL SALIVA STRIP TEST Dianita Apriyanti; Siti Nurfajriah; Maulin Inggraini; Noor Andryan Ilsan
Jurnal Mitra Kesehatan Vol. 5 No. 2 (2023): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v5i2.185

Abstract

Alcohols are organic compounds containing hydroxyl functional groups. The alcohol content in the drink is ethanol. Alcohol poisoning is often associated with injuries from falls, fires, drowning, overdose, sexual abuse, work accidents, traffic accidents, and abusive relationship. This study aims to determine the level of alcohol in alcoholics using the Alcohol Saliva Strip Test method. The type of research used is descriptive with purposive sampling technique and cross-sectional approach and. Specimen collection and examination of the alcohol content in the respondent's saliva were carried out in March 2021 at Kp X, Depok City, West Java Province. The number of respondents was 28 people. The inclusion criteria for this study was alcoholic drinkers aged 17-25 years. The study exclusion criteria were using mouthwash, consuming ascorbic acid such as vitamin C, and having a history of gout. The specimen used in this research was saliva. The method is detected for alcohol content of the respondents used the RightSign brand Alcohol Saliva Strip Test. The results showed the age of the respondents who drank the most alcohol in the range of 17-19 years was 57%. The characteristics of respondents based on the frequency of alcohol consumption are highest in the frequent category (3 times a week or more) as much as 68%. The results showed 71% of the respondents positively drank alcohol and 29% of the respondents were negative. The lowest alcohol content in the respondent's saliva was 0.02% and the highest was 0.08%. The alcohol content in the saliva of the respondents who last drank alcohol 1 week ago was undetectable. This study concludes that the percentage of respondent who detected alcohol in their saliva was 71% and the highest alcohol content in the respondent's saliva is 0.08%. This study also shows that the period, alcohol concentration, and type of alcohol consumed can influence Blood Alcohol Concentration (BAC).
Increasing Public Awareness Related To Early Detection Of Diabetes Mellitus In Jatimekar Bekasi Village Maulin inggraini; Noor Andryan Ilsan; Siti Nurfajriah; Ria Amelia; Elfira Maya Sari
Jurnal Masyarakat Religius dan Berwawasan Vol 2, No 1 (2023): Masyarakat Religius dan Berwawasan
Publisher : Universitas Islam Negeri Mahmud Yunus Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31958/mrw.v2i1.8703

Abstract

Noncommunicable Diseases (NCDs) are a serious threat not only for the elderly but also for the young. Based on a report from the International Diabetes Federation (IDF) by 2022 that 1.57 million of the 8.75 million people living with type 1 diabetes worldwide in 2022 are less than 20 years old. This shows the importance of increasing public awareness about Diabetes Mellitus (DM). This PKM is carried out by providing education about the prevention and dangers of DM, then followed by a Temporary Blood Sugar (TBS) examination in the Jatimekar Bekasi village using brochure media. The increas in participants’s knowledge was carried out by analyzing the pre-test and post-test descriptively. The results of the study showed an increase in public knowledge about DM. this can be seen from the increase in post-test result compared to pre-test scores. GDS examination results include risk characteristics of 86.96% (110-200 mg/dL) and DM 13.04 % (>200 mg/dL). There are 3 counseling participants who suffer from DM and have a history of unhealthy eating patterns and not doing physical activity.
EDUKASI DAN PELATIHAN PEMERIKSAAN INFEKSI SALURAN KEMIH (ISK) PADA SISWA SMK TEKNOLOGI LABORATORIUM MEDIS (TLM) DI KOTA BEKASI Reza Anindita; Siti Nurfajriah; Ria Amelia; Noor Andryan Ilsan; Maulin Inggraini; Elfira Maya Sari
Jurnal Abdi Insani Vol 10 No 4 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i4.1180

Abstract

Urinary Tract Infection (UTI) is an infectious disease caused by bacterial colonization in the urinary tract. The bacteria most commonly found in UTI patients is E. coli. UTI is more common in women (79%) than men (21%) aged 17-35 years. The problem of UTIs causes an increase in morbidity (illness) rates, thereby increasing medical costs. Therefore, early prevention efforts are needed, one of which is through education and the introduction of UTI examinations through Community Service (PKM) activities for secondary school students. The aim of this PKM is to provide simple education and training regarding UTI examination for vocational school students majoring in TLM in Bekasi City. This PKM also provides students with experience in carrying out UTI examinations in the laboratory. This activity was carried out at the family partner STIKES with a total of 43 students majoring in TLM vocational schools. This activity stage includes planning, implementation and evaluation. Data analysis was carried out using descriptive and comparative tests for 2 samples using a paired t-test. The results of this activity showed that there were 37 (86.04%) female participants while there were 6 (13.95%) male participants. The average pre-test score was 3.48 while the post-test was 8.76 or an increase of 15.17%. The results of the paired t-test showed a significant difference between the pre-test and post-test with a significance value of (0.306<0.05). The conclusion of this PKM is that an evaluation using test instruments (pre test and post test) in the form of 10 multiple choice questions was able to significantly increase knowledge about UTI for 43 students of the TLM Department of Health Vocational Schools in Bekasi City.
Pengujian Kemampuan Larva Ulat Bambu (Omphisa fuscidentalis) sebagai Hewan Uji Virulensi Bakteri Klebsiella pneumoniae Inggraini, Maulin; Ilsan, Noor Andryan; Romadhona, Vega Aulia; Anindita, Reza
JURNAL BIOSHELL Vol 12 No 2 (2023): Oktober
Publisher : Universitas Islam Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/bio.v12i2.2317

Abstract

Pneumonia dapat menyebabkan inflamasi berlebihan pada paru-paru maupun inflamasi secara sistemik sehingga bisa menyebabkan kematian pada penderita. Bakteri utama penyebab pneumonia diantaranya Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae dan Klebsiella pneumoniae. K. pneumoniae memiliki faktor-faktor virulensi yang dapat mempengaruhi tingat keparahan penyakit. Pengujian virulensi suatu bakteri dapat dilakukan salah satunya dengan cara in vivo menggunakan hewan uji. Hewan uji yang umum digunakan adalah tikus putih (Rattus norvegicus), mencit (Mus musculus), nematoda transparan (Caenorhabditis elegans), ikan zebra (Danio rerio), ngengat lilin (Galleria mellonela) dan ulat bambu (Omphisa fuscidentalis). Tujuan dari penelitian ini adalah untuk menguji kemampuan Omphisa fuscidentalis sebagai hewan alternatif untuk menguji virulensi Klebsiella pneumoniae. Jenis penelitian yang digunakan adalah kuantitatif dengan desain penelitian eksperimental. Perlakuan yang dilakukan berupa penyuntikkan Klebsiella pneumoniae ke larva Omphisa fuscidentallis dengan konsentrasi 104, 105, 106 CFU/larva. Semua perlakuan dilakukan sebanyak 10 kali pengulangan. Larva O.fuscidentalis diamati pola kematiannya sampai jam ke 72. Hasil yang didapat adalah terdapat pengaruh antara konsentrasi K. pneumoniae dengan jumlah kematian larva ulat O.fuscidentalis. Uji virulensi K. pneumoniae pada konsentrasi 106 CFU/larva adalah konsentrasi yang tercepat dalam membunuh O.fuscidentalis, pada jam ke 24 sejumlah 8 larva dengan kategori 2 Mati Putih (MP), 5 Mati Coklat (MC) dan 1 Mati Hitam (MH). Kata kunci: Hewan uji, Klebsiella pneumoniae, larva ulat bambu, Omphisa fuscidentalis, pneumonia.
Antimicrobial Resistance and Molecular Identification of Clinical Multi-Drug Resistant Enterobacter Cloacae Fajriah, Siti Nur; Inggraini, Maulin; Ilsan, Noor Andryan; Anindita, Reza
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.334

Abstract

Enterobacter cloacae is a Gram-negative bacteria causing nosocomial infections. This bacteria has increased resistance to various antibiotics in the past five years, resulting in a multi-drug-resistant (MDR) phenotype. In particular, MDR E. cloacae causes longer hospitalization time, increases medical costs, and affects morbidity and mortality. This study aimed to observe the minimum inhibitory concentration (MIC) of clinical E. cloacae towards several antibiotics and molecular identification of MDR E.cloacae. This study was conducted in a descriptive design. Secondary data was collected at the microbiology laboratory of the Teaching Hospital in Bekasi, Indonesia, from May to September 2020. Sampel was carbapenem resistant E.cloacae. The isolate was originated from a human clinical specimen, then was confirmed molecular identification using 16s rRNA. In this study, only one carbapenem-resistant E. cloacae, which is also MDR bacteria, was found. This E. cloacae was categorized as MDR bacteria since it was resistant to more than three antibiotic classes, including carbanemen, extended-spectrum cephalosporin, penicillins + β lactamase inhibitor, antipseudomonal penicillins + β lactamase inhibitor aminoglycoside, and penicillin. Vitek 2 identification of this isolate was E. cloacae complex. It showed similar results to molecular identification based on a partial sequence of 16s rRNA. BLASTn result of the trimmed sequence was E. cloacae with 99.78 % similarity.
Virulence Evaluation of Aeromonas spp. KS-1 Isolated from Kitchen Sponge using Omphisa fuscidentalis Larvae Ilsan, Noor Andryan; Inggrain, Maulin; Nurfajriah, Siti; Yunita, Melda; Priyanto, Jepri Agung; Ramanda, Viqih
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.613-620

Abstract

Aeromonas spp. causes the human diseases including diarrhea, gastroenteritis, and bacteremia. Aeromonas spp. can be found in kitchen sponge, one of the reservoirs for food-borne bacterial pathogens. Virulence study of Aeromonas spp. in vivo in animal model is important since the animal model can mimic manifestasions in human infections. Omphisa fuscidentalis was chosen for alternative virulence model, since they are in the same taxonomical order with the well-known infections model, Galleria mellonella. Bacterial isolation and selection of kitchen sponge used Brain Heart Infusion agar and Endo Agar, respectively. Bacterial virulence of KS-1 was injected into Omphisa fuscidentalis larvae. Survival percentage and melanization score of infected larvae were evaluated. Hemolymph of larvae with melanization score of 1 and 4 were stained with Giemsa method to observe the hemocyte changes. Bacterial identification of isolate KS-1 based on 16S rRNA sequence resulted in 96.9% identity to Aeromonas spp. strain VS7. Isolate KS-1 injection to O. fuscidentalis revealed higher bacterial dosage resulting more severe symptoms to the larvae according to survival percentage and melanization score. However, statistical analysis showed evaluation of melanization score could distinguish larvae with 106 and 107 CFU/larva dosage injection, while evaluation of survival percentage could not. Hemocyte of larvae with melanization score 1 had larger and more cytoplasmic vacuolization than the score 4 (healthy larvae). Omphisa fuscidentalis is an alternative of insect model for bacterial infections with survival percentage and melanization score as the evaluation. Cytoplasmic vacuolization of hemocyte can be used as larvae’s health indicator in a cellular level.
The Identification of Escherichia coli Virulence Gene from Diabetic Foot Ulcer (DFU) at the Diabetes Care Home, Bekasi, West Java, Indonesia Inggraini, Maulin; Anindita, Reza; Ilsan, Noor Andryan; Nurfajriah, Siti
BIOVALENTIA: Biological Research Journal Vol. 11 No. 1 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.1.2025.448

Abstract

One of the complications of diabetes is diabetic foot ulcer (DFU). One of the bacteria that shows a high prevalence in DFU patients is Escherichia coli. The purpose of this study to determine the virulence gene in E.coli isolated from DFU patients at the Bekasi Diabetes Care Home, West Java, Indonesia. Detection of E.coli virulence genes was carried out using the Whole Genome Sequence (WGS) method. Data analysis was carried out descriptively. This study showed the presence of fdeC, fimH, astA, hlyE, hlyF, csgA, and cvaC genes. This study concluded that the fdeC and fimH genes encode protein expression for the formation of colonisation or biofilm, astA, hlyE, and hlyF genes encode the expression of enterotoxin and hemolysin, csgA genes, and cvaC encode the expression of colicin, Curlin, and microcin C bacteriocin.
Co-Authors Agustin, Yola Violita Amelia, Ria Dianita Apriyanti Dinar Ikhwal Prasetia Elfira Maya Sari Euodia Naomi Septiana Siburian Fajriah, Siti Nur Farida Ariyani Ika Nurfitrianti Inggrain, Maulin Inggraini, Maulin Inggriani, Maulin Jepri Agung Priyanto, Jepri Agung Liendrie Patria Elsa Maulin Inggraini Maulin Inggriani Maulin Inggriani Melda Yunita Nurfitrianti, Ika Prasetia, Dinar Ikhwal Ramanda, Viqih Reza Anindita Romadhona, Vega Aulia Sayyid Daffa Alfath Sipriyadi Siti Nurfajriah Siti Nurfajriah Yola Violita Agustin