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PELATIHAN UJI SENSITIVITAS BAKTERI TERHADAP ANTIBIOTIK PADA SISWA SMK KESEHATAN BHAKTI KARTINI, BEKASI, JAWA BARAT Anindita, Reza; Inggraini, Maulin; Ilsan, Noor Andryan
Jurnal Abdi Insani Vol 12 No 6 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i6.2549

Abstract

Salah satu skema sertifikasi okupasi Asisten Teknisi yang disusun oleh Komite Badan Nasional Sertifikasi Profesi laboratorium medik LSP P 1 siswa/i jurusan Teknologi Laboratorium Medis (TLM) SMK Bhakti Kartini adalah melakukan pengujian mikrobiologi, khususnya uji sensitivitas bakteri terhadap antibiotik. Namun, keterbatasan alat dan bahan di Laboratorium mikrobiologi TLM SMK Bhakti Kartini menyebabkan ketrampilan tersebut hanya diberikan secara simulasi dalam bentuk alat peraga.  Tujuan dari kegiatan PKM ini adalah memberikan pelatihan mengenai uji sensitivitas bakteri terhadap antibiotik dengan metode Kirby Baeur (paper disk) pada siswa/i TLM SMK Bhakti Kartini di Kota Bekasi, Jawa Barat. Kegiatan PKM ini meliputi meliputi persiapan, pelatihan antibiotik dan resistensi bakteri melalui ceramah, demonstrasi, praktikum, serta evaluasi melalui pre-post test dan ujian keterampilan yang dianalisis secara deskriptif kuantitatif dan uji t-test berpasangan. Hasil PKM ini menunjukkan peserta yang hadir berjumlah 37 siswa/i kelas 12 TLM. Nilai rata-rata pre test sebesar 61 setelah dilakukan post test menjadi 77 atau mengalami peningkatan sebesar  26,22%, sedangkan skor nilai rata-rata ketrampilan sebesar 73,7. Kesimpulan pada PKM ini semua peserta telah memiliki pengetahuan mengenai konsep antibiotik dan resistensi bakteri terhadap antibiotik, serta telah terampil dalam melakukan uji sensitivitas bakteri terhadap antibiotik dengan metode kirby baeur (paper disk) yang ditunjukkan dengan hasil t-test paired untuk nilai signifikansi ujian konsep sebesar 0.01 <0.05 dan rata-rata nilai ujian ketrampilan sebesar 73,7.
Uji Kepekaan Antibiotik Klebsiella pneumoniae dari Sampel Sputum, Darah, dan Pus Inggraini, Maulin; Ilsan, Noor Andryan; Nurfajriah, Siti
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.555

Abstract

AKTIVITAS INHIBITOR ALFA GLUKOSIDASE DARI AKTINOMISET ASAL PHYLLOPLANE KELOR (Moringa oleifera) Noor Andryan Ilsan; Siti Nurfajriah; Maulin Inggriani
Bioma Vol. 14 No. 2 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.257 KB) | DOI: 10.21009/Bioma14(2).1

Abstract

The exploration of alternative natural products is one of the important focus for researcher. Microbes are still the best source for searching the bioactive compounds. Actinomycetes are Gram Positive bacteria which have been known able to produce various of bioactive compounds such as antimicrobials and enzyme inhibitors. The aims of this study were to isolate Actinomycetes from Moringa oleifera phyllosphere also evaluate the antimicrobial and alpha glucosidase inhibitor activities. The result of molecular 16s rRNA gene identification showed FKU 1 isolate had closest related to Nocardia rhamnosiphila. FKU 1 isolate had antimicrobial activities against E.coli, Staphylococcus aureus, Klebsiella pneumonia, K. pneumonia ESBL, dan Vibrio sp. in vitro. FKU 1 also had alpha glucosidase inhibitor activity of 7.50 ± 1.59% at 0.01 µg mL-1 extract concentrations of compared to acarbose (control) of 8.41 ± 0.43%.
Increasing Public Awareness Related To Early Detection Of Diabetes Mellitus In Jatimekar Bekasi Village inggraini, Maulin; Andryan Ilsan, Noor; Nurfajriah, Siti; Amelia, Ria; Maya Sari, Elfira
MARAWA: Jurnal Masyarakat Religius dan Berwawasan Vol 2 No 1 (2023): Masyarakat Religius dan Berwawasan
Publisher : Universitas Islam Negeri Mahmud Yunus Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31958/mrw.v2i1.8703

Abstract

Noncommunicable Diseases (NCDs) are a serious threat not only for the elderly but also for the young. Based on a report from the International Diabetes Federation (IDF) by 2022 that 1.57 million of the 8.75 million people living with type 1 diabetes worldwide in 2022 are less than 20 years old. This shows the importance of increasing public awareness about Diabetes Mellitus (DM). This PKM is carried out by providing education about the prevention and dangers of DM, then followed by a Temporary Blood Sugar (TBS) examination in the Jatimekar Bekasi village using brochure media. The increas in participantsÔÇÖs knowledge was carried out by analyzing the pre-test and post-test descriptively. The results of the study showed an increase in public knowledge about DM. this can be seen from the increase in post-test result compared to pre-test scores. GDS examination results include risk characteristics of 86.96% (110-200 mg/dL) and DM 13.04 % (>200 mg/dL). There are 3 counseling participants who suffer from DM and have a history of unhealthy eating patterns and not doing physical activity.
Identification and characterization of bacteria from feces and river in East Bekasi using biochemical test Ilsan, Noor Andryan; Maulin Inggraini; Liendrie Patria Elsa; Sayyid Daffa Alfath
Bioma Vol. 21 No. 1 (2025): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/bioma.v21i1.49141

Abstract

Identification and characterization of bacteria are the two main detection in determining the species level of pathogenicity of a bacteria. This study aims to isolate bacteria from normal person faeces, liquid faeces, and some sourced from river water which is still used by local communities for bathing, swimming and fishing activities, the results of which are consumed. Water contaminated with microorganisms such as bacteria is most commonly caused by contamination from pollution, industrial waste, even animal and human faeces. The media used was selective differential Salmonella Shigella Agar (SSA) media. Colonies that grown were then characterized by testing their biochemical activity. The results of the biochemical tests were compared with Bergey's Manual of Determinative Bacteriology. From seven samples for each location, Salmonella sp. was identified from J. Kh Abu Bakar river, and E. coli was identified from Bumi Palapa river. Both of these species were categorized as human pathogens. The rest of samples were contained the opportunistic bacteria, including Enterobacter sp. and Serratia sp. This study concluded that two from seven samples location were high risk for human health.This identification can help to determine the pathogens that cause various diseases with a large impact on society.