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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Chimica et Natura Acta Bioma : Jurnal Biologi Indonesia Biota Jurnal Kesehatan Islam : Islamic Health Journal Jurnal Abdi Insani Jurnal Mitra Kesehatan JURNAL BIOSHELL BIOVALENTIA: Biological Research Journal Jurnal Masyarakat Religius dan Berwawasan
Ilsan, Noor Andryan
Department Of Medical Laboratory Technology, STIKes Mitra Keluarga, Margahayu, Bekasi, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences

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AKTIVITAS INHIBITOR ALFA GLUKOSIDASE DARI AKTINOMISET ASAL PHYLLOPLANE KELOR (Moringa oleifera) Noor Andryan Ilsan; Siti Nurfajriah; Maulin Inggriani
Bioma Vol. 14 No. 2 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.257 KB) | DOI: 10.21009/Bioma14(2).1

Abstract

The exploration of alternative natural products is one of the important focus for researcher. Microbes are still the best source for searching the bioactive compounds. Actinomycetes are Gram Positive bacteria which have been known able to produce various of bioactive compounds such as antimicrobials and enzyme inhibitors. The aims of this study were to isolate Actinomycetes from Moringa oleifera phyllosphere also evaluate the antimicrobial and alpha glucosidase inhibitor activities. The result of molecular 16s rRNA gene identification showed FKU 1 isolate had closest related to Nocardia rhamnosiphila. FKU 1 isolate had antimicrobial activities against E.coli, Staphylococcus aureus, Klebsiella pneumonia, K. pneumonia ESBL, dan Vibrio sp. in vitro. FKU 1 also had alpha glucosidase inhibitor activity of 7.50 ± 1.59% at 0.01 µg mL-1 extract concentrations of compared to acarbose (control) of 8.41 ± 0.43%.
The Identification of Escherichia coli Virulence Gene from Diabetic Foot Ulcer (DFU) at the Diabetes Care Home, Bekasi, West Java, Indonesia Inggraini, Maulin; Anindita, Reza; Ilsan, Noor Andryan; Nurfajriah, Siti
BIOVALENTIA: Biological Research Journal Vol. 11 No. 1 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.1.2025.448

Abstract

One of the complications of diabetes is diabetic foot ulcer (DFU). One of the bacteria that shows a high prevalence in DFU patients is Escherichia coli. The purpose of this study to determine the virulence gene in E.coli isolated from DFU patients at the Bekasi Diabetes Care Home, West Java, Indonesia. Detection of E.coli virulence genes was carried out using the Whole Genome Sequence (WGS) method. Data analysis was carried out descriptively. This study showed the presence of fdeC, fimH, astA, hlyE, hlyF, csgA, and cvaC genes. This study concluded that the fdeC and fimH genes encode protein expression for the formation of colonisation or biofilm, astA, hlyE, and hlyF genes encode the expression of enterotoxin and hemolysin, csgA genes, and cvaC encode the expression of colicin, Curlin, and microcin C bacteriocin.
PELATIHAN UJI SENSITIVITAS BAKTERI TERHADAP ANTIBIOTIK PADA SISWA SMK KESEHATAN BHAKTI KARTINI, BEKASI, JAWA BARAT Anindita, Reza; Inggraini, Maulin; Ilsan, Noor Andryan
Jurnal Abdi Insani Vol 12 No 6 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i6.2549

Abstract

Salah satu skema sertifikasi okupasi Asisten Teknisi yang disusun oleh Komite Badan Nasional Sertifikasi Profesi laboratorium medik LSP P 1 siswa/i jurusan Teknologi Laboratorium Medis (TLM) SMK Bhakti Kartini adalah melakukan pengujian mikrobiologi, khususnya uji sensitivitas bakteri terhadap antibiotik. Namun, keterbatasan alat dan bahan di Laboratorium mikrobiologi TLM SMK Bhakti Kartini menyebabkan ketrampilan tersebut hanya diberikan secara simulasi dalam bentuk alat peraga.  Tujuan dari kegiatan PKM ini adalah memberikan pelatihan mengenai uji sensitivitas bakteri terhadap antibiotik dengan metode Kirby Baeur (paper disk) pada siswa/i TLM SMK Bhakti Kartini di Kota Bekasi, Jawa Barat. Kegiatan PKM ini meliputi meliputi persiapan, pelatihan antibiotik dan resistensi bakteri melalui ceramah, demonstrasi, praktikum, serta evaluasi melalui pre-post test dan ujian keterampilan yang dianalisis secara deskriptif kuantitatif dan uji t-test berpasangan. Hasil PKM ini menunjukkan peserta yang hadir berjumlah 37 siswa/i kelas 12 TLM. Nilai rata-rata pre test sebesar 61 setelah dilakukan post test menjadi 77 atau mengalami peningkatan sebesar  26,22%, sedangkan skor nilai rata-rata ketrampilan sebesar 73,7. Kesimpulan pada PKM ini semua peserta telah memiliki pengetahuan mengenai konsep antibiotik dan resistensi bakteri terhadap antibiotik, serta telah terampil dalam melakukan uji sensitivitas bakteri terhadap antibiotik dengan metode kirby baeur (paper disk) yang ditunjukkan dengan hasil t-test paired untuk nilai signifikansi ujian konsep sebesar 0.01 <0.05 dan rata-rata nilai ujian ketrampilan sebesar 73,7.
Co-Authors Agustin, Yola Violita Amelia, Ria Dianita Apriyanti Dinar Ikhwal Prasetia Elfira Maya Sari Euodia Naomi Septiana Siburian Fajriah, Siti Nur Farida Ariyani Ika Nurfitrianti Inggrain, Maulin Inggraini, Maulin Inggriani, Maulin Jepri Agung Priyanto, Jepri Agung Maulin Inggraini Maulin Inggriani Maulin Inggriani Melda Yunita Nurfitrianti, Ika Prasetia, Dinar Ikhwal Ramanda, Viqih Reza Anindita Romadhona, Vega Aulia Sipriyadi Siti Nurfajriah Siti Nurfajriah Yola Violita Agustin