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Effects of Red Coconut Tembuluk (Cocos nucifera var. rubescens) Extract on Creatinine Level Reduction in Mice (Mus musculus) Amelia A Riuh; Sutrisnawati Sutrisnawati; Manap Trianto; Masrianih Masrianih; Fatmah Dhafir; I Nengah Kundera
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.699-704

Creatinine is a key biochemical indicator of renal function, and elevated creatinine levels reflect impaired kidney performance. Carbon tetrachloride (CCl4) is a known nephrotoxic agent that induces oxidative stress and renal damage. This study evaluate the effect of red coconut tembuluk (Cocos nucifera var. rubescens) extract on creatinine in CCl4-induced mice (Mus musculus). The study employed an experimental laboratory design using 24 male mice, which were divided into six groups: normal control, negative control (CCl4-induced), and four treatment groups administered red coconut tembuluk extract at concentrations of 10%, 20%, 40%, and 80%. Renal damage was induced by intraperitoneal injection of CCl4 at a dose of 1 mL/kg body weight. The extract was administered orally for eight consecutive days following induction. Serum creatinine levels were measured before and after treatment. The results showed that CCl4 induction significantly increased creatinine levels in the negative control group compared to the normal control, indicating impaired renal function. Administration of red coconut tembuluk extract resulted in a significant reduction in creatinine levels across all treatment groups, with the most pronounced effect observed at the 80% concentration. Statistical analysis using one-way ANOVA demonstrated a significant effect of the extract on creatinine reduction (p < 0.05), followed by a Least Significant Difference (LSD) test confirming differences among treatment groups. The nephroprotective effect of the extract is likely associated with its bioactive compounds, including flavonoids, antioxidants, and L-arginine, which contribute to the reduction of oxidative stress and support renal tissue recovery. In conclusion, red coconut tembuluk extract effectively reduces serum creatinine levels in CCl4-induced mice, with the 80% concentration showing the greatest protective effect. These findings suggest that red coconut tembuluk has potential as a natural nephroprotective agent.

Phylogenetic Analysis of Sulawesi Endemic Butterfly Papilio polytes Using the COI (Cytochrome Oxidase I) Gene Andi Nurjannah; Lestari MP Alibasyah; Manap Trianto; Masrianih Masrianih; Yulia Windarsih; Amalia Buntu
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.705-713

Papilio polytes is a butterfly belonging to the family Papilionidae and is endemic to Sulawesi. The evolutionary relationships of this species were investigated through phylogenetic analysis based on mitochondrial DNA, specifically the COI (Cytochrome Oxidase I) region, which was subsequently reconstructed into a phylogenetic tree. This study aimed to analyze the phylogenetic relationships of P. polytes collected from Bakubakulu Village, Palolo District, Sigi Regency, Central Sulawesi, in relation to other members of the genus Papilio from different regions. Specimens of P. polytes were collected using a roaming (exploratory) method, followed by DNA isolation, DNA amplification, sequencing, electrophoresis, and bioinformatics analyses using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The phylogenetic tree analyses using the Neighbor Joining (NJ) and Maximum Likelihood (ML) methods revealed clear evolutionary relationships. Papilio polytes from Central Sulawesi showed a closer genetic relationship with P. polytes from other regions and clustered within the same clade, whereas other species formed separate clades. Genetic distance analysis based on the COI gene indicated that P. polytes from Central Sulawesi exhibited a genetic distance of 0.00% among individuals, suggesting very high genetic similarity within the population. The genetic distance between P. polytes from Central Sulawesi and P. polytes populations from other regions was relatively low, ranging from 0.00% to 3.86%, while the distance to the outgroup species Eurytides marcellus and Battus polydamas was much higher, ranging from 13.15% to 16.25%. These findings indicate that the phylogenetic relationships among P. polytes populations are very close and consistent.

Identification of Food Sources of Apis cerana Fabricius, 1793 Based on Pollen Analysis Moh Syahrial Farawansyah; Lestari MP Alibasyah; Manap Trianto; Masrianih Masrianih; Yulia Windarsih; Amalia Buntu
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.723-730

Apis cerana commonly known as the “Asian honey bee,” has been utilized for thousands of years across Asia, its native region, for honey production and pollination services. This species is often described as the closest “twin” of its European/African sister species, A. mellifera. This study aimed to identify plant species serving as pollen food sources for the honey bee A. cerana based on pollen morphological analysis. The research was conducted using a descriptive exploratory approach in Jononunu Village, Parigi Tengah District, Parigi Moutong Regency, Central Sulawesi, Indonesia. Pollen samples were collected from worker bees during foraging activities and subsequently analyzed through acetolysis and microscopic examination in the laboratory. Pollen identification was based on morphological characteristics, including exine ornamentation, aperture type, and pollen size.The results showed that A. cerana utilized pollen sources from 14 plant species belonging to 12 families, indicating a high diversity of forage resources. The identified pollen morphological characteristics comprised four types of exine ornamentation, six aperture types, and pollen sizes ranging from very small to large, with small- to medium-sized pollen being dominant. Tricolporate aperture types and psilate ornamentation were the most frequently observed characteristics. Based on plant growth form, pollen sources were predominantly derived from tree species, followed by herbs, while shrubs and bushes contributed less. These findings provide important baseline information for melissopalynological studies and may serve as a reference for the management and conservation of A. cerana and its forage plants.

Abundance and Distribution of Millipedes (Diplopoda) in Wera Nature Recreation Park Rifana Lemba; Syech Zainal; Rafiqa Rafiqa; Fatmah Dhafir; Masrianih Masrianih; Manap Trianto
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.597-603

Millipedes (Diplopoda) play an important role in forest ecosystems, particularly in the decomposition of organic matter and the recycling of nutrients, thereby contributing to soil fertility and ecosystem stability. This study aimed to determine the abundance and distribution patterns of millipedes (Diplopoda) in Wera Nature Recreation Park, Balumpewa Village, West Dolo District, Sigi Regency, Central Sulawesi. The research was designed as a quantitative descriptive study. Sampling was carried out using an exploratory survey method combined with hand collection techniques at three observation stations. These stations were selected using purposive sampling based on differences in environmental characteristics and habitat conditions. Data collection was conducted in three repetitions at two-week intervals to obtain representative data on millipede communities. In addition to species collection, measurements of physical environmental parameters were also performed, including light intensity, soil pH, air temperature, and humidity, as these factors are known to influence the presence and distribution of soil macrofauna. The results showed that six millipede species were recorded, belonging to three orders and three families. The average environmental conditions at the study site included a light intensity of 722.2 lux, soil pH of 6.8, temperature of 28.2°C, and humidity of 76.7%. These conditions indicate a habitat that is relatively less optimal for millipede populations. The species abundance index was classified as low, suggesting limited population sizes within the study area. However, the Relative Abundance Index (RAI) revealed variation among species, with two species categorized as having high relative abundance, one species moderate, and three species low. Analysis using Morisita’s Index and the Standardized Morisita Index (Ip) demonstrated that all recorded species exhibited a clumped distribution pattern. This distribution suggests that millipedes tend to concentrate in specific microhabitats that provide suitable moisture, shelter, and food resources. Overall, the findings indicate that unstable environmental conditions may restrict millipede distribution, causing them to utilize only particular microhabitats that meet their ecological requirements.

Plant Lice Species (Suborder: Sternorrhyncha) on Ornamental Plants in Kampal Village and Surrounding Areas Delia Nurhaliza; Masrianih Masrianih; Manap Trianto; Amalia Buntu; Yulia Windarsih; Lestari MP Alibasyah
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.505-512

Plant lice (Suborder: Sternorrhyncha) are important pests of ornamental plants, capable of causing physical damage and transmitting plant pathogens. These insects pose a significant threat as they can reduce growth, impair physiological functions, and even cause plant death. Their feeding activity affects leaves, stems, and flowers, thereby reducing overall plant health and aesthetic value. Understanding the diversity and host associations of these pests is essential for effective management. This study aimed to identify the species of Sternorrhyncha plant lice present on ornamental plants in Kampal Village, Parigi Moutong, Central Sulawesi. A qualitative descriptive approach was employed, using purposive sampling to collect specimens from different plant parts, including shoots, leaves, flowers, and stems. Observations were recorded and analyzed to determine species composition, host range, and infestation patterns. A total of 25 ornamental plant species belonging to 16 families were observed as host plants. Some plant families, such as Euphorbiaceae, Acanthaceae, Rubiaceae, Palmae, Rutaceae, Araceae, and Agavaceae, were infested by more than one plant louse family, while individual plant species could host multiple louse families simultaneously. Seventeen Sternorrhyncha species were identified, distributed across seven families: Pseudococcidae (6 species), Coccidae (3), Diaspididae (2), Aphididae (2), Margarodidae (2), and Aleyrodidae and Ortheziidae (1 species each). These results provide baseline data on the diversity and distribution of Sternorrhyncha plant lice in Kampal Village. Such information is critical for developing targeted pest management strategies to protect ornamental plants and maintain their health and aesthetic value in residential and cultivated areas.

Effects of Red Coconut Tembuluk (Cocos nucifera var. rubescens) Extract on Uric Acid Level Reduction in Mice (Mus musculus) Sukmawati Sukmawati; Sutrisnawati Sutrisnawati; Manap Trianto; Masrianih Masrianih; Fatmah Dhafir; I Nengah Kundera
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.693-698

Increased levels of uric acid in the blood can lead to metabolic disorders such as gouty arthritis, which require effective management. This study aimed to determine the effect of red coconut tembuluk extract (Cocos nucifera var. rubescens) on reducing uric acid levels in mice (Mus musculus). The study employed an experimental research design using a Completely Randomized Design (CRD). A total of 28 mice were used, divided into seven groups with four replications each. The groups consisted of a normal control group (KN), which received food and water ad libitum; a negative control group (K+); a positive control group (K-); and four treatment groups (PI, PII, PIII, and PIV). The negative control group (K+), positive control group (K-), and all treatment groups were induced with lard oil for 14 days to increase uric acid levels. The positive control group (K?) was treated with allopurinol at a dose of 0.026 mg per 20 g body weight. The normal control (KN) and negative control (K+) groups received food and water ad libitum without extract administration. Treatment group PI received 10% red coconut tembuluk extract, PII received 20%, PIII received 40%, and PIV received 80%. The data obtained were analyzed using Analysis of Variance (ANOVA). The results showed that administration of red coconut tembuluk extract significantly reduced uric acid levels in mice induced with lard oil. The most effective concentration in reducing uric acid levels was 80% (PIV), with an average uric acid level of 5.4 mg/dL.

Total Flavonoid and Tannin Content in Pumpkin Stem Extract (Cucurbita moschata) Vadillah Vadillah; Sutrisnawati Sutrisnawati; Manap Trianto; Fatmah Dhafir; Zulaikhah Dwi Jayanti; Masrianih Masrianih
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1113-1119

Cucurbita moschata is a medicinal plant that possesses various pharmacological properties, including antidiabetic, antifungal, antibacterial, anti-inflammatory, and antioxidant activities, which are associated with its bioactive compound content. One plant part that has been relatively underutilized is the pumpkin stem, although it has the potential to contain secondary metabolites such as flavonoids and tannins. This study aimed to identify and determine the levels of bioactive compounds in pumpkin stem extract based on stem maturity stages, namely young stems and mature stems, using both qualitative and quantitative methods. Qualitative analysis was conducted through phytochemical screening using color reaction methods with specific reagents to detect flavonoids and tannins. Quantitative analysis was performed using UV-Vis spectrophotometry based on standard calibration curves. The results showed that pumpkin stem extract tested positive for both flavonoids and tannins. Quantitative analysis indicated that the flavonoid content in young stems was 7.22, which was higher than in mature stems (3.00). In contrast, tannin content was higher in mature stems (9.03) compared to young stems (5.02). These differences indicate that flavonoids are more dominant in young tissues with active metabolic activity, whereas tannins tend to accumulate in mature tissues as part of plant defense mechanisms. Based on these findings, it can be concluded that pumpkin stem extract contains flavonoid and tannin compounds with varying levels depending on stem maturity, suggesting its potential as a natural source of antioxidants.

Phylogenetic Analysis of Schistosoma japonicum Based on 16S rRNA Gene Safani Safani; Sutrisnawati Sutrisnawati; Manap Trianto; I Nengah Kundera; Zulaikhah Dwi Jayanti; Masrianih Masrianih
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1161-1170

Schistosomiasis is a neglected tropical disease caused by trematode worms of the genus Schistosoma and remains a public health concern in several endemic areas of Central Sulawesi, Indonesia. DNA-based molecular approaches have been increasingly applied to support species identification and the analysis of genetic relationships in Schistosoma japonicum. This study aimed to investigate the phylogenetic relationships and genetic variation of S. japonicum based on the mitochondrial 16S rRNA gene. Samples of Oncomelania hupensis lindoensis were collected from endemic areas surrounding Lake Lindu, Central Sulawesi, Indonesia. Detection of S. japonicum was performed using the crushing method, followed by DNA extraction with the GS 100gSYNCTM DNA Extraction Kit. Amplification of the 16S rRNA gene was carried out using the Polymerase Chain Reaction (PCR) technique with the primers LR13107-F and LR12647-R. Sequence data were analyzed using BLAST, MEGA 11, MESQUITE, DNASTAR, GeneStudio, and DnaSP software. Phylogenetic trees were reconstructed using the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods with 10,000 bootstrap replicates. PCR amplification produced clear and high-quality DNA bands. BLAST analysis revealed query cover values ranging from 99.90% to 100% and sequence identity of 100% with Schistosoma japonicum sequences available in GenBank. Genetic variation analysis indicated low genetic diversity, characterized by two haplotypes, one variable site, haplotype diversity of 0.500 ± 0.170, and nucleotide diversity of 0.00055 ± 0.00015. Nucleotide composition analysis showed that thymine (T) was the most abundant nucleotide (43.53%), and the A+T content (72.14%) was higher than the G+C content (27.87%), a characteristic feature of mitochondrial genomes. Phylogenetic reconstruction using both NJ and ML methods consistently clustered the Central Sulawesi samples with S. japonicum populations from Shanghai and Guangdong, China, with strong bootstrap support values ranging from 99% to 100%. Genetic distance analysis based on the Kimura 2-Parameter model revealed genetic divergence values of 0.00–0.00% among Central Sulawesi samples and between the Indonesian and Chinese populations, indicating very close genetic relationships and high genetic homogeneity. These findings demonstrate that S. japonicum populations in Central Sulawesi exhibit low genetic variation and share close phylogenetic relationships with other Asian populations, providing valuable molecular information to support the development of schistosomiasis surveillance and control strategies in Indonesia.

Population Density and Distribution Pattern of Golden Apple Snail (Pomacea canaliculata L.) in the Rice Field Ecosystem Sinta Sinta; Sutrisnawati Sutrisnawati; Manap Trianto; Masrianih Masrianih; Rafiqah Rafiqah; Fatmah Dhafir
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1121-1127

The golden apple snail (Pomacea canaliculata) is a freshwater mollusc species commonly found in rice field ecosystems and is recognized as one of the major pests of rice crops. This species possesses a high adaptive capacity and a rapid reproductive rate, allowing its population to increase rapidly and cause significant damage to rice plants. Nevertheless, the golden apple snail also has ecological roles and potential benefits, including serving as an alternative feed source for livestock and fish, as well as contributing to the decomposition of organic matter in aquatic environments. This study aimed to analyze the population density and distribution pattern of the golden apple snail in the rice fields of Dolago Padang Village, South Parigi District. The research employed a quantitative descriptive approach using a field survey method. Data were collected using a quadrat sampling technique with plot sizes of 2 × 2 m established at three different observation stations. The number of golden apple snail individuals within each plot was counted directly and subsequently analyzed to determine population density and distribution patterns using the Morisita Index. In addition, environmental parameters, including water temperature and pH, were measured. The results showed that a total of 90 golden apple snail individuals were recorded during the study. Population density ranged from 1 to 2 individuals/m², which was categorized as moderate. The distribution pattern of the golden apple snail at all observation stations was classified as uniform, as indicated by Morisita Index values below 1 (Id < 1). Environmental conditions, with temperatures ranging from 27–30°C and pH values between 6.5 and 7.2, were within the optimal range supporting the presence and development of the golden apple snail.

Diversity of Butterfly (Suborder: Rhopalocera) in the Kapopo Grand Forest Park Area, Central Sulawesi Murni Ammalia; Abd Hakim Laenggeng; Manap Trianto; Masrianih Masrianih; Amalia Buntu; Fatmah Dhafir
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1079-1086

Butterflies are important components of biodiversity, serving as pollinators and environmental bioindicators. This study aimed to assess butterfly diversity (Suborder Rhopalocera) in the Kapopo Grand Forest Park, Central Sulawesi, Indonesia. A quantitative descriptive approach was employed using purposive sampling across three observation stations representing high-vegetation, low-vegetation, and riverine habitats. Data were collected through field surveys conducted during morning and afternoon observation periods, and species were identified based on morphological characteristics. Species diversity was analyzed using the Shannon–Wiener diversity index. A total of 26 butterfly species belonging to three families Nymphalidae, Pieridae, and Papilionidae were recorded, comprising 323 individuals. Nymphalidae was the most species-rich family with 13 species, followed by Pieridae (7 species) and Papilionidae (6 species). The Shannon–Wiener diversity index (H') was 1.5, indicating a moderate level of species diversity (1 <= H' <= 3). This result suggests that habitat conditions within the Kapopo Grand Forest Park remain relatively stable and suitable for sustaining butterfly populations. Variations in habitat characteristics among observation stations, particularly differences in vegetation structure and levels of human disturbance, influenced the distribution and abundance of butterfly species throughout the study area.

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