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Penerapan Teknik Cawan Tuang dan Sebar Dalam Pemeriksaan Enterobacteriaceae pada Pasien Diare Sebagai Upaya Peningkatan Kualitas Diagnosis Laboratorium Essydhea Hasfika; Diah Prihatiningsih; Moh. Fairuz Abadi
Jurnal Medika: Medika in progres
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/wyt38555

Abstract

Diare masih menjadi permasalahan kesehatan masyarakat yang signifikan dan salah satu penyebab utamanya adalah infeksi bakteri dari famili Enterobacteriaceae. Pemeriksaan mikrobiologi melalui metode kultur merupakan langkah penting dalam mendukung diagnosis dan pengendalian penyakit diare. Kegiatan ini bertujuan untuk membandingkan jumlah Enterobacteriaceae pada sampel feses penderita diare menggunakan teknik cawan tuang (pour plate) dan cawan sebar (spread plate). Kegiatan ini menggunakan desain eksperimental laboratoris dengan pendekatan komparatif terhadap 16 sampel feses penderita indikasi diare yang dianalisis di laboratorium. Data diperoleh melalui penghitungan jumlah koloni bakteri dalam satuan CFU/gram, kemudian dianalisis secara deskriptif dan statistik menggunakan uji Shapiro–Wilk dan uji Wilcoxon Signed-Rank. Hasil penelitian menunjukkan bahwa metode cawan tuang menghasilkan rata-rata jumlah Enterobacteriaceae yang lebih tinggi dan lebih homogen dibandingkan metode cawan sebar. Temuan ini menunjukkan bahwa metode cawan tuang lebih efektif dalam menghitung total populasi Enterobacteriaceae pada sampel feses penderita diare, sedngkan metode cawan sebar lebih sesuai untuk pengamatan morfologi koloni permukaan. Disarankan untuk melibatkan jumlah sampel yang lebih besar dan beragam guna meningkatkan validitas serta generalisasi hasil penelitian.
PERBEDAAN HASIL PEMERIKSAAN MALARIA METODE RDT DENGAN PENGGUNAAN MIKROPIPET DAN PIPET TETES DALAM PENUANGAN SPESIMEN Saputro Panca Sakti; Moh. Fairuz Abadi; Ni Wayan Desi Bintari
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar belakang: Malaria merupakan penyakit menular yang tetap menjadi tantangan kesehatan masyarakat di Indonesia, sehingga dibutuhkan metode diagnosis yang cepat dan akurat. Rapid Diagnostic Test (RDT) banyak digunakan sebagai metode deteksi, namun keakuratannya dapat dipengaruhi oleh alat yang digunakan dalam penanganan spesimen darah, seperti mikropipet dan pipet tetes. Tujuan: membandingkan hasil pemeriksaan malaria metode RDT menggunakan kedua alat tersebut. Penelitian pra-eksperimental ini dilakukan di Puskesmas Karubaga, Kabupaten Tolikara, Provinsi Papua Pegunungan, dengan 30 spesimen darah pasien bergejala klinis malaria yang dipilih melalui purposive sampling. Metode: Analisis data menggunakan uji Chi-Square untuk mengidentifikasi perbedaan hasil antara penggunaan mikropipet dan pipet tetes. Hasil: analisis menunjukkan nilai p = 1,000 (p > 0,05), yang mengindikasikan tidak terdapat perbedaan signifikan antara kedua metode. Meskipun demikian, penggunaan mikropipet cenderung menghasilkan tampilan garis merah muda pada strip RDT yang lebih jelas dan konsisten dibandingkan pipet tetes. Kesimpulan: pentingnya pemilihan alat yang tepat guna mendukung keakuratan interpretasi hasil pemeriksaan malaria.
HUBUNGAN FREKUENSI KONSUMSI FAST FOOD DENGAN INDEKS MASSA TUBUH (IMT) PADA REMAJA PUTRI Maria Kristina Natalia Jalla; Ni Wayan Suniyadewi; Moh. Fairuz Abadi
Jurnal Penelitian Keperawatan Vol 12 No 2 (2026): Jurnal Penelitian Keperawatan
Publisher : STIKES RS Baptis Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32660/jpk.v12i2.1010

Abstract

Perubahan gaya hidup remaja di wilayah perkotaan mendorong meningkatnya konsumsi makanan cepat saji (fast food), yang berpotensi memengaruhi status gizi dan Indeks Massa Tubuh (IMT). Meskipun hubungan konsumsi fast food dan IMT telah banyak dilaporkan, kajian yang berfokus pada remaja putri di lingkungan sekolah perkotaan Bali masih terbatas. Pendekatan kuantitatif cross sectional diterapkan pada 218 siswi SMA Negeri 4 Denpasar yang dipilih melalui simple random sampling. Frekuensi konsumsi fast food diukur menggunakan kuesioner, sedangkan IMT ditentukan melalui pengukuran antropometri. Analisis data menggunakan uji Rank Spearman menunjukkan sebagian besar responden memiliki konsumsi fast food kategori sedang (58,7%) dan IMT normal (66,5%). Terdapat hubungan signifikan antara frekuensi konsumsi fast food dan IMT (p < 0,001). Frekuensi konsumsi fast food yang lebih tinggi berhubungan dengan peningkatan IMT pada remaja putri.
HUBUNGAN INTENSITAS PENGGUNAAN MEDIA SOSIAL DENGAN KESEHATAN MENTAL REMAJA DI SMPK 1 HARAPAN DENPASAR Made Ayu Abrilia Oktavianti Dewi; Ni Kadek Yuni Lestari; Moh. Fairuz Abadi
Jurnal Medicare Vol. 5 No. 3 (2026): JULY 2026 (INPRESS)
Publisher : Rena Cipta Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62354/jurnalmedicare.v5i3.384

Abstract

The increasing intensity of social media use among adolescents has the potential to affect their mental health. This study aimed to analyze the relationship between the intensity of social media use and adolescents' mental health at SMPK 1 Harapan Denpasar. This study employed a quantitative descriptive correlational design with a cross-sectional approach. A total of 180 adolescents were selected using a purposive sampling technique. Data were collected using a social media use intensity questionnaire and the Self-Reporting Questionnaire (SRQ-20) and were analyzed using Spearman's rank correlation test. The results showed that most respondents had a moderate level of social media use (99.4%) and did not experience mental health problems (68.9%). Spearman's rank correlation analysis revealed a significant relationship between the intensity of social media use and mental health (p = 0.001; r = 0.517). It can be concluded that the intensity of social media use is significantly associated with adolescents' mental health. Therefore, continuous education on the responsible use of social media should be strengthened through the involvement of families, schools, and healthcare professionals.
Perbadingan Hasil Pemeriksaan pH dan Leukosit Menggunakan Urin Segar dan Urin Tunda 2 Jam pada Pasien Infeksi Saluran Kemih (ISK) dengan Metode Carik Celup Anjela Stefanie Natalia; Moh. Fairuz Abadi; Putu Gede Subhaktiyasa
Jaringan Laboratorium Medis Vol 7 No 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13196

Abstract

Urinary Tract Infection (UTI) is one of the most prevalent diseases that requires rapid and accurate laboratory diagnosis. Urine examination using dipstick methods serves as a practical diagnostic alternative. However, the stability of urine specimens subjected to delayed examination needs further evaluation, particularly regarding pH and leukocyte parameters. This study aimed to determine the differences in urine pH and leukocyte results between fresh urine and urine stored for 2 hours at room temperature using the dipstick method in UTI patients. This experimental study employed a quantitative approach. A total of 30 urine samples were collected from UTI patients at RSAD Tk. II Udayana. Each sample was analyzed under two conditions: immediately (fresh urine) and after 2 hours of storage at room temperature (delayed urine), yielding a total of 60 measurements. The results showed an increase in the median urine pH from 6.0 to 6.5 and a decrease in leukocyte count from a median of 125 to 70 after 2 hours of storage at room temperature, with statistically significant differences (p < 0.05). These findings indicate that delaying urine examination for 2 hours can affect pH and leukocyte values, potentially leading to inaccurate diagnostic interpretation. Therefore, urine chemical examination should be performed within one hour after collection or stored at 2–8 °C to maintain result accuracy.
Hubungan Indeks Massa Tubuh dengan Kadar Glukosa Darah Sewaktu pada Mahasiswa Teknologi Laboratorium Medis Program Diploma Tiga STIKES Wira Medika Bali Ni Made Dian Anjaswari; Moh. Fairuz Abadi; Didik Prasetya
Jaringan Laboratorium Medis Vol 7 No 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13197

Abstract

Body Mass Index (BMI) is a simple parameter used to assess a person's nutritional status, whether they are underweight or overweight. Overweight is often associated with the risk of elevated blood glucose levels (hyperglycemia), which can develop into metabolic syndrome. This condition is increasingly found in adolescents and young adults with unhealthy lifestyles. Therefore, this study was conducted to determine the relationship between BMI and random blood glucose levels (RBG) in regular students of the Diploma III Medical Laboratory Technology program at Wira Medika Bali Health College. This study employed an observational analytical method with a cross-sectional approach. A sample of 30 students was selected through purposive sampling based on inclusion criteria: aged 15–24 years, not taking medications that affect blood sugar levels, and willing to participate. BMI data were obtained through weight and height measurements, then categorized into underweight, normal weight, overweight, obesity I, and obesity II. RBG examinations were performed using capillary blood with the aid of a glucometer. Analysis of the relationship between variables used the Spearman Rank Test. The results showed that the BMI distribution of respondents was 13.3% underweight, 40.0% normal weight, 13.3% overweight, 23.4% obesity I, and 10.0% obesity II. All respondents had random blood glucose levels <200 mg/dL, thus remaining within the normal range. Statistical testing showed a p-value of 0.996 (p>0.05), concluding that there was no significant relationship between BMI and GDS in students. The conclusion of this study is that BMI has not been proven to be directly related to random blood glucose levels. However, weight control remains necessary as a long-term preventive measure against metabolic syndrome and other non-communicable diseases associated with obesity.
Comparison of Serum Creatinine Levels between Immediate and Delayed Examination at Room Temperature Gisel Audina Tandea; Moh. Fairuz Abadi; Ni Luh Nova Dilisca Dwi Putri
Jurnal Kesehatan Cendikia Jenius Vol. 3 No. 3 (2026): Agustus 2026
Publisher : CV. CENDIKIA JENIUS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70920/jenius.v3i3.463

Abstract

Background: Serum creatinine examination is an important parameter for assessing kidney function; however, its stability is strongly influenced by examination time and sample storage conditions . Delayed examination at room temperature may cause alterations in creatinine levels, thus requiring further investigation. Objective: This study aimed to determine the difference in serum creatinine levels between immediate and delayed examination at room temperature. Methods: A quantitative descriptive study with a retrospective approach and a cross-sectional design was conducted on 57 positive blood culture specimens using a total sampling technique. Data from January to December 2025 were analyzed descriptively using frequency distributions and percentages. Result: The results showed that the mean creatinine level decreased from 1.19 mg/dL (immediate) to 0.99 mg/dL (2 hours) and 0.92 mg/dL (4 hours). Statistical analysis indicated a significant difference (p<0.05). Conclusion: It can be concluded that delayed examination affects serum creatinine levels; therefore, prompt examination is recommended to ensure result accuracy.