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All Journal MEDISAINS Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Narra J International Journal of Health and Pharmaceutical (IJHP)
Handayani, Irda
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Analysis of procalcitonin and urine nitrite to predictor sepsis patients Maghfirah, Andi Itha; Muhiddin, Rachmawati; Handayani, Irda; Kadir, Nursin Abdul
MEDISAINS Vol 19, No 2 (2021)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v19i2.11075

Abstract

Background: Sepsis is a significant health problem worldwide. Various sepsis biomarkers have been studied before, but few data link PCT and urinary nitrite levels to culture result in septic patients.Purposes: This study compares PCT, Urine Nitrite on culture results against their sensitivity to determine variables that can be used to predict sepsis patients.Methods: This is a cross-sectional study by taking data on patients with sepsis aged ≥18 years in 207. Diagnostic tests were performed to determine sensitivity and specificity of PCT, Urine Nitrite, and Blood Culture of septic patients. A Chi-square test was carried out to see the relationship between two parameters and the outcome of sepsis.Results: PCT sensitivity was higher than Urine Nitrite: 74.5%. Specificity of Urine Nitrite was higher than PCT: 88.2%. Procalcitonin has a significant relationship with sepsis patient outcomes (p<0.005).Conclusion: PCT can predict septic patients because it has a higher sensitivity than urine nitrite
Analysis Neutrophil-to-Lymphocyte and Monocyte-to- Lymphocyte Ratios in Pediatric Respiratory Infections Suraidah, Suraidah; Handayani, Irda; Kadir, Nursin Abdul
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 1 (2024)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i1.2271

Abstract

           Indonesia ranks third among countries with the highest tuberculosis (TB) cases worldwide. Pneumonia also stands as a leading cause of death among infants in developing nations. The delay in diagnosing and the challenges in distinguishing TB and pneumonia have significant impacts on elevated morbidity and mortality rates. Both Neutrophil-to-Lymphocyte Ratio (NLR) and Monocyte-to-Lymphocyte Ratio (MLR) serve as inflammatory biomarkers utilized for rapid and straightforward bacteremia evaluation. This study aimed to analyze the difference between NLR and MLR in diagnosing of TB and pneumonia in children. This study was retrospective cross-sectional research utilizing secondary data at the time of initial diagnosis by a clinician as TB or pneumonia. Subjects with TB and/or pneumonia were treated at Dr. Wahidin Sudirohusodo Hospital, Makassar from January 2017 to December 2021. The research sample consisted of 150 patients with pediatric patients with pneumonia. Statistical analyses involved the Kolmogorov-Smirnov test, Mann-Whitney U test, and Receiver Operating Characteristic (ROC). This study examined NLR and MLR as supportive biomarkers for diagnosing TB and pneumonia. The NLR (AUC 0.674) and MLR (AUC 0.63) values in TB subjects were reasonably good in distinguishing between TB diagnosis and healthy subjects. The NLR (AUC 0.77) and MLR (AUC 0.787) values were effective in distinguishing pneumonia diagnosis from healthy control with better sensitivity and specificity compared to TB subjects. However, NLR (AUC 0.401) and MLR (AUC 0.384) values were not recommended to distinguish pneumonia from tuberculosis due to low AUC and extremely low sensitivity and specificity. The NLR and MLR values cannot be used to differentiate TB and pneumonia in children due to their low sensitivity and specificity.
Computational drug repurposing for tuberculosis by inhibiting Ag85 complex proteins Iskandar, Israini W.; Nurhasanah, Astutiati; Hatta, Mohammad; Hamid, Firdaus; Handayani, Irda; Chaera, Ummi; Yusriyyah, Andi A.; Jamaluddin, Balqis D.; Zaenab, St; Hidayah, Najdah; Karimah, Nihayatul; Permana, Andi D.; Massi, Muhammad N.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1130

Abstract

Tuberculosis (TB) remains a significant and deadly infection among pulmonary diseases caused by Mycobacterium tuberculosis, a highly adaptive bacterium. The ability of M. tuberculosis to evade certain drugs has been linked to its unique structure, particularly in the cell envelope, where the Ag85 complex proteins play an essential role in this part.  The aim of this study was to utilize a drug repurposing strategy targeting the Ag85 complex proteins. This study utilized a computational approach with 120 selected drugs experimentally identified to inhibit Tuberculosis. A virtual screening molecular docking with Autodock Vina was used to filter the compounds and identify the strong binders to the Ag85 Complex. Molecular dynamics simulations employed the Gromacs Packages to evaluate the stability of each complex, including root mean square deviation (RMSD), root mean square fluctuation (RMSF), and radius of gyration (RoG). Additionally, absorption, distribution, metabolism, excretion, and toxicity (ADMET) assessments were conducted to gather more information about the drug-likeness of each hit compound. Three compounds, selamectin, imatinib, and eltrombopag were selected as potential drugs repurposed to inhibit the activity of the Ag85 complex enzyme, with binding affinities ranging between -10.560 kcal/mol and -11.422 kcal/mol. The MD simulation within 100 ns (3 replicas) showed that the average RMSD of each Ag85A complex was 0.15 nm–0.16 nm, RMSF was 0.09 nm–0.10 nm, and RoG was 1.80 nm–1.81 nm. For Ag85B, the average RMSD was 1.79 nm–1.80 nm, RMSF was 0.08 nm–0.09 nm, and RoG was 1.79 nm – 1.80 nm. Then, for Ag85C, the mean RMSD was 0.16 nm–0.18 nm, RMSF was 0.09, and RoG was 1.77 nm. The study highlights that these promising results demonstrate the potential of some repurposed drugs in combating the Ag85 complex.
Bacterial Identification and Antibiotic Sensitivity Tests of COVID-19 Patients at ICU Wahidin Sudirohusodo Hospital Ampulembang, Diana Tangdan; Handayani, Irda; Abdul Kadir, Nursin
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 2 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i2.2275

Abstract

The use of antibiotics in COVID-19 pneumonia patients admitted to the ICU with various risk factors can increase the possibility of antibiotic resistance. Irrational antibiotic use during the pandemic is one of the causes of antibiotic resistance. This study aimed to describe bacterial identification and antibiotic resistance in pneumonia patients admitted to the COVID-19 and non-COVID-19 ICU Dr. Wahidin Sudirohusodo Hospital. This study was a descriptive observational study with a cross-sectional method. Data were collected from medical records from Dr. Wahidin Sudirohusodo Hospital for the period January 2020-December 2022. The population that met the inclusion criteria were pneumonia patients admitted to the COVID ICU and non-COVID ICU and had pneumonia thoracic photographs, positive culture results, and antibiotic sensitivity tests. A total of 532 patients diagnosed with COVID-19 and 53 non-COVID-19 were treated in the ICU. Samples that met the inclusion criteria were 77 subjects with COVID-19 with 87 culture specimens and 36 non-COVID-19 patients with 47 culture specimens. The most common types of bacteria were Gram-negative bacteria in the COVID-19 (70.1%) and non-COVID-19 (78.7%) groups, Acinetobacter baumannii were primarily found in non-COVID-19 (29.8%) and COVID-19 patients (14.9%). The most common sensitivity test result was MDR in the COVID-19 (65.5%) and non-COVID-19 (72.3%) groups. The most common antibiotic resistance in both groups were Cephalosporins, Carbapenems, and Fluoroquinolones against Acinetobacter baumannii. Antibiotics that remained sensitive to Acinetobacter baumannii were Amikacin and Gentamicin. Bacterial identification results of COVID-19 and non-COVID-19 patients showed dominance of Gram-negative bacteria. The antibiotic sensitivity test showed MDR as the most common result.
Analysis of Systemic Immune-Inflammation Index (Sii) And Cd4 Count In Hiv/Aids Patients With And Without Tuberculosis Co-Infection Fathiyah, Riza; Paramita, Kartika; Handayani, Irda
International Journal of Health and Pharmaceutical (IJHP) Vol. 5 No. 2 (2025): May 2025 ( Indonesia - Iraq - Malaysia)
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijhp.v5i2.386

Abstract

Background: The Global Incidence Of Human Immunodeficiency Virus (Hiv) Infection Continues To Rise, With Increased Mortality Observed In The Presence Of Opportunistic Infections Such As Tuberculosis (Tb), Torch Infections (Toxoplasmosis, Rubella, Cytomegalovirus, Herpes Simplex), And Candidiasis. The Systemic Immune-Inflammation Index (Sii), Derived From Neutrophil, Lymphocyte, And Platelet Counts Obtained Through Routine Complete Blood Count Testing, Has Recently Been Explored As A Potential Marker Of Immune Response And Inflammation In Hiv Patients, Particularly In Those With Tb Co-Infection. Objectives: To Analyze The Sii Index And Cd4 Count In Hiv Patients With And Without Tb Co-Infection. Methods: This Retrospective Study Analyzed Secondary Data From Hiv-Infected Patients Treated At Wahidin Sudirohusodo Hospital, Makassar, Between January 2022 And June 2024. The Mann–Whitney U Test Was Used To Compare Sii And Cd4 Counts Between Groups, And Receiver Operating Characteristic (Roc) Curve Analysis Was Performed To Assess The Predictive Value Of Sii For Tb Co-Infection. Results: Among 144 Patients, Sii Values Were Significantly Higher In The Hiv-Tb Co-Infection Group Compared To The Non-Tb Group (P = 0.001). Roc Analysis Revealed That An Sii Cut-Off Value Of 562.3 Yielded A Sensitivity Of 82.4% And A Specificity Of 35.5%, With An Area Under The Curve (Auc) Of 0.661, Indicating Weak Discriminatory Power. Cd4+ T Cell Counts Were Significantly Lower In Patients With Tb Co-Infection (P < 0.001). Conclusion: Sii Was Significantly Elevated In Hiv Patients With Tb Co-Infection, Demonstrating Relatively High Sensitivity And Suggesting Its Potential Utility As A Screening Tool For Identifying Tb Co-Infection In Hiv-Infected Individuals. Cd4+ T Cell Counts Were Also Significantly Lower In The Co-Infection Group, Consistent With Greater Immunosuppression.
Co-Authors Abdul Kadir, Nursin Ampulembang, Diana Tangdan ASTUTIATI NURHASANAH Chaera, Ummi Fathiyah, Riza Hamid, Firdaus Hatta, Mohammad Hidayah, Najdah Iskandar, Israini W. Jamaluddin, Balqis D. Kadir, Nursin Abdul Karimah, Nihayatul Kartika Paramita Maghfirah, Andi Itha Massi, Muhammad N. Muhiddin, Rachmawati Permana, Andi D. Suraidah Suraidah, Suraidah Yusriyyah, Andi A. Zaenab, St