Garuda - Garba Rujukan Digital

The Potency of Pluchea indica Less as Preventive Effort to Lead (Pb) Pertubation on Rats (Rattus norvegicus) Organs Aulanni'am, Aulanni'am; Julianto, Andri; Dewi, Made Artari; Dirgahariyawan, Tri Cahyo; Mahdi, Chanif; Wuragil, Dyah Kinasih; Herawati, Herawati
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Lead (Pb) is one of the heavy m metals polluting the environment which is harmful to t the organism. Pb accumulation triggers free radic cals and disrupts the body's metabolism. Pluche indic ca leaf extract is reported contain flavonoids whiich act as antioxidants and can counteract free radiccal attacks. The purpose of this study was to de etermine the role of Pluchea indica leaf extract preve entive therapy in counteracting free radicals due too exposure to Pb in rats (Rattus norvegicus). Wistar mmale rats aged of 8-10 weeks with a body weight of about 200-250 grams. Rats were divided into 5 tr reatment groups, were group A as negative contr rol, group B as positive control induced by lead (Pb)) 10 mg/rat/day, groups C,D,E given with ethano olic extract of Pluche indica leavesof 200 mg/kgBW, , 400 mg/kgBW, and 600 mg/kgBW and lead (Pb b) exposure of 10mg/ rat/day. Kidney, liver and jejunaal histopathology using HE staining was observed microscopically. The results showed that the administtration of Pluche indica leaves extract could preve ent tissue damage to the kidney, liver and jejunum in rats exposured to Pb. Dose of 600 mg/kgBW show wed histopathological improvement in kidney, liver annd jejunum. The greater the dose, the better prev ention of tissue damage as indicated by tissue condition resembling those of control rats. The conclusion of this study, the Pluchea indica leaf extract has the potential to prevent organ damaged exposed to lead.

THE STUDY OF ORGANOPHOSPHATE (DIAZINON) TOXICITY TOWARD LIVER HISTOPATHOLOGY AND MALONDIALDEHYDE (MDA) SERUM LEVELS ON RATS (Rattus norvegicus) Pratama, Dyah Ayu Oktavianie; Aulia, Zulfa; Aulanni'am, Aulanni'am; Permata, Fajar Shodiq
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Diazinon is an insecticide that has a higher toxicity than other insecticides. Normally, insecticides are detoxified by liver, but this process produced free radicals which causes cell damage. Free radicals also increase lipid peroxidation which directly increase malondialdehyde levels. This research was aimed to determine the influence of diazinon toxicity to the liver histopathology and levels of malondialdehyde (MDA) in the serum of rats (Rattus norvegicus). The rats were divided into 4 groups which consist of negative control group and three experimental groups which were given diazinon 20 mg/kgBW (P1), 40 mg/ kgBW (P2), and 60 mg/ kgBW (P3). The rats were given diazinon for 8 weeks orally. The parameters used in this research was liver histopathology with hematoxilin eosin stain and Malondialdehyde levels with Thiobarbituric test. Liver histopathology was analyzed in descriptive qualitative and the level of MDA was analyzed quantitatively using ANOVA and Tukey's exact test with Î± = 0.05. The result of this research showed the influence of organophosphate (diazinon) on liver histolopathology was shown by inflammatory cells infiltration in hepatic parenchyma, sinusoidal congestion, and cytoplasmic vacuolation of the hepatocytes. Statistical analysis proved that the diazinon was able to increase the level of MDA serum significantly (p<0,05) up to 71% in experimental group P1 (dose 20 mg/kgBW), which the increase level of MDA serum depend on dose of diazinon. This research concluded that diazinon is one of the organophosphate pesticide that toxic based on liver histopathology and MDA serum levels.Â

Possible Role of Akt in Mossy Fiber Sprouting: Akt Activity and CA3 Mossy Fiber Sprouting in a Kainate Model of Epilepsy: Akt Activity and CA3 Mossy Fiber Sprouting in Epilepsy husna, Machlusil; Handono, Kusworini; Sujuti, Hidayat; Aulanni'am, Aulanni'am
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.09

The most prevalent pathological phenomenon observed in patients with epilepsy is hippocampal mossy fiber sprouting (MFS), which is thought to be associated with epileptic progression, such as worsening seizure control, cognitive function, and behavior. MFS is discovered in the dentate gyrus and the hippocampal Cornu Ammon 3 (CA3) area. The CA3 area is involved in memory, so disturbances in that area can affect memory impairment in patients with epilepsy. The mammalian target of rapamycin complex-1 (mTORC1) is also associated with MFS. Akt is an upstream activator of mTORC1 and a downstream target of mammalian target of rapamycin complex-2 (mTORC2) and plays a role in cytoskeleton organization. We analyzed Akt activity and MFS in the CA3 zone in an in vitro model of kainate-induced epilepsy. We divided organotypic hippocampal slice cultures (OHSCs) into a kainate (epilepsy) group and a control (untreated) group. On the 10th day in vitro (DIV), the kainate group was exposed to 8 µM kainic acid for 48 h, diluted in the medium. At 32 DIV, we measured Akt activity through western blotting and CA3 MFS through synaptoporin fluorescence intensity observed using confocal laser scanning microscopy. We found that Akt activity increased significantly (p = 0.000) in the kainate group, and the synaptoporin fluorescence intensity also increased in the stratum oriens of the CA3 area (p = 0.049) in the kainate group. Our findings implied that Akt may play a role in MFS development. Because Akt is a main downstream target of mTORC2, mTORC2 may also be involved in MFS development. Further research is required to clarify these findings.

Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) Hanifwati, Annisa; Marhendra, Agung Pramana Warih; Aulanni'am, Aulanni'am
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2019.009.02.12

Olive oil (Olea europaea) contains 55 to 83% oleic acid which is a single chain unsaturated fatty acid or mono-unsaturated fatty acid (MUFA), and 2% phenolic components in the form of hydroxytyrosol and tyrosol. This study aims to determine the potential of olive oil extract (Olea europaea) in reducing cholesterol and malondialdehyde levels, along with inhibiting fatty liver development in hiperlipemic rats. Sixteen rats were divided into four groups, the first group was healthy control group, the second group was positive control group, received a high-fat diet containing 100 mg of cholesterol powder, 25 mg of cholic acid powder and 1 mL of quail egg yolk for 28 days. Third and fourth treatment groups were received for 28 days, plus 0.828 g (1 mL) and 1.656 g (2 mL) of olive oil extract daily for 14 days, respectively. The measurement of total cholesterol used cholesterol test strips based on oxidation enzyme reaction cholesterol esterase and cholesterol oxidase that produce hydrogen peroxidation, then analyzed with biosensor refractophotometry. Measurement of malondialdehyde used thiobarbituric acid (TBA) test. Histological observation of fatty liver was assessed using a NAS (Non-alcoholic fatty liver disease score). The results showed that 1.656 g (2 mL) of olive oil extract per day decreased total cholesterol level up to 44.41 %, malondialdehyde level up to 61.75%, and NAS score up to 50%, compared with positive control. It was concluded that olive oil extract was decreasing total cholesterol level, as an anti-oxidant and prevent fatty liver development.Keywords: fatty liver, hyperlipidemia, malondialdehyde, Olea europaea.

Possible Role of Akt in Mossy Fiber Sprouting: Akt Activity and CA3 Mossy Fiber Sprouting in a Kainate Model of Epilepsy: Akt Activity and CA3 Mossy Fiber Sprouting in Epilepsy husna, Machlusil; Handono, Kusworini; Sujuti, Hidayat; Aulanni'am, Aulanni'am
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.09

The most prevalent pathological phenomenon observed in patients with epilepsy is hippocampal mossy fiber sprouting (MFS), which is thought to be associated with epileptic progression, such as worsening seizure control, cognitive function, and behavior. MFS is discovered in the dentate gyrus and the hippocampal Cornu Ammon 3 (CA3) area. The CA3 area is involved in memory, so disturbances in that area can affect memory impairment in patients with epilepsy. The mammalian target of rapamycin complex-1 (mTORC1) is also associated with MFS. Akt is an upstream activator of mTORC1 and a downstream target of mammalian target of rapamycin complex-2 (mTORC2) and plays a role in cytoskeleton organization. We analyzed Akt activity and MFS in the CA3 zone in an in vitro model of kainate-induced epilepsy. We divided organotypic hippocampal slice cultures (OHSCs) into a kainate (epilepsy) group and a control (untreated) group. On the 10th day in vitro (DIV), the kainate group was exposed to 8 µM kainic acid for 48 h, diluted in the medium. At 32 DIV, we measured Akt activity through western blotting and CA3 MFS through synaptoporin fluorescence intensity observed using confocal laser scanning microscopy. We found that Akt activity increased significantly (p = 0.000) in the kainate group, and the synaptoporin fluorescence intensity also increased in the stratum oriens of the CA3 area (p = 0.049) in the kainate group. Our findings implied that Akt may play a role in MFS development. Because Akt is a main downstream target of mTORC2, mTORC2 may also be involved in MFS development. Further research is required to clarify these findings.

Colchicine attenuates chemical hypoxia-induced pyroptosis through downregulation of nuclear factor kappa B and caspase-1 in cardiomyocytes Satrijo, Budi; Rohman, Mohammad S.; Aulanni'am, Aulanni'am; Sujuti, Hidayat; Lestari, Bayu
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.2245

Myocardial infarction (MI) is the leading cause of mortality worldwide. During MI, cardiomyocyte necrosis and inflammation are crucial in the post-MI cardiac remodeling process, including pyroptosis. Although colchicine is a well-known anti-inflammatory drug that has been clinically studied in the context of MI, its role in cardiac pyroptosis remains unclear. The aim of this study was to investigate the role of colchicine in pyroptosis in vitro, using CoCl2-induced H9c2 cells. Prior to the primary experiment, the hypoxic model in H9c2 cells was optimized by evaluating hypoxia-inducible factor-1 alpha (HIF-1α) expression and viability in cells exposed to various concentrations of CoCl2 at different time intervals. Subsequently, an in vitro hypoxia model was established by treating H9c2 cells with CoCl2 (600 µM), with or without colchicine (1 µM), for 3 hours. Flow cytometry was used to measure the expression of nuclear factor-kappa beta (NF-κB), interleukin 18 (IL-18), caspase-1, and HIF-1α in pyroptotic cells. Immunofluorescence was used to assess caspase-1 localization and its colocalization with propidium iodide during late-stage pyroptosis. Our data indicated that CoCl2-induced hypoxia significantly upregulated NF-κB, caspase-1, and IL-18 expression, and increased pyroptotic cell death in H9c2 cells. Colchicine treatment attenuated these effects, leading to a marked reduction in NF-κB, caspase-1, and IL-18 expression in hypoxic cells. Colchicine treatment significantly decreased the number of late pyroptotic cells. The protective effect of colchicine was more pronounced in late hypoxia (24-hour) setting compared to early hypoxia (3-hour). These findings suggest that colchicine attenuates cardiac pyroptosis in hypoxic H9c2 cells, as evidenced by the significant downregulation of key proteins involved in this pathway, including NF-κB, caspase-1, and IL-18. This protective effect appeared to be more effective in late hypoxia.

Characterization of TPO Enzyme and its Immunogenecity in New Zealand White Rabbits (Oryctolagus cuniculus) Ramadani, Devi; Wijaya, Andreas Budi; Srihardyastutie, Arie; Wuragil, Dyah Kinasih; Rosandi, Rulli; Rudijanto, Achmad; Aulanni'am, Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9539

Hypothyroidism is characterized by inadequate production of thyroid hormones, chiefly thyroxine (T4) and triiodothyronine (T3). Thyroid peroxidase (TPO), is an enzyme and crucial for producing thyroid hormones, and also serves as a primary autoantigen in autoimmune thyroid conditions, such as Hashimoto's thyroiditis and Graves' disease. The existence of anti-thyroid peroxidase antibodies (anti-TPO) indicates an autoimmune response in which the immune system TPO which is normally recognized as part of the body (self), begins to be identified by the immune system as a something foreign (non-self). This study aimed to characterize, measure TPO activity, and evaluate the immunogenicity of TPO protein derived from patient serum and its ability to induce TPO antibody production in New Zealand White Rabbits. TPO protein isolation was achieved through SDS-PAGE and confirmed by western blot technique have molecular weight of 52 kDa. In samples from normal patients, the average TPO enzyme activity was 31.34 U/mL, while in hypothyroid patients, the average activity increased to 49.46 U/mL. TPO polyclonal antibody production in rabbits indicated its immunogenicity, peaking in week eight post-immunization, with an ELISA absorbance of 0.555. Dot blot analysis further confirmed anti-TPO sensitivity at various dilutions, anti-TPO has a high sensitivity at 1:80 dilution still detects TPO in sera patient.

Title

Found 7 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 7 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 7 Documents
Search