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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Veterinary Biomedical and Clinical Journal Jurnal Pengabdian, Pendidikan dan Teknologi
Wuragil, Dyah Kinasih
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Social Sciences Veterinary Other

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The Potency of Pluchea indica Less as Preventive Effort to Lead (Pb) Pertubation on Rats (Rattus norvegicus) Organs Aulanni'am, Aulanni'am; Julianto, Andri; Dewi, Made Artari; Dirgahariyawan, Tri Cahyo; Mahdi, Chanif; Wuragil, Dyah Kinasih; Herawati, Herawati
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.161 KB) | DOI: 10.21776/ub.VetBioClinJ.2021.001.01.1

Abstract

Lead (Pb) is one of the heavy m metals polluting the environment which is harmful to t the organism. Pb accumulation triggers free radic cals and disrupts the body's metabolism. Pluche indic ca leaf extract is reported contain flavonoids whiich act as antioxidants and can counteract free radiccal attacks. The purpose of this study was to de etermine the role of Pluchea indica leaf extract preve entive therapy in counteracting free radicals due too exposure to Pb in rats (Rattus norvegicus). Wistar mmale rats aged of 8-10 weeks with a body weight of about 200-250 grams. Rats were divided into 5 tr reatment groups, were group A as negative contr rol, group B as positive control induced by lead (Pb)) 10 mg/rat/day, groups C,D,E given with ethano olic extract of Pluche indica leavesof 200 mg/kgBW, , 400 mg/kgBW, and 600 mg/kgBW and lead (Pb b) exposure of 10mg/ rat/day. Kidney, liver and jejunaal histopathology using HE staining was observed microscopically. The results showed that the administtration of Pluche indica leaves extract could preve ent tissue damage to the kidney, liver and jejunum in rats exposured to Pb. Dose of 600 mg/kgBW show wed histopathological improvement in kidney, liver annd jejunum. The greater the dose, the better prev ention of tissue damage as indicated by tissue condition resembling those of control rats. The conclusion of this study, the Pluchea indica leaf extract has the potential to prevent organ damaged exposed to lead.
Management of Avian Influenza H9N2 Vaccination in Broiler Chickens at JAPFA COMFEED Myanmar Pte Ltd. Verian, Bagas Ridjalu; Prasetyo, Dodik; Adrenalin, Sruti Listra; Wuragil, Dyah Kinasih; Cahyo, Budi Nur
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.3

Abstract

Avian influenza virus subtype H9N2 (AIV-H9N2) has been a concern for poultry health in the last 20 years. The virus emerged in domestic poultry in the mid-1990s and has been reported in several Asian countries, including Myanmar. AI subtype H9N2 caused huge economic losses to the poultry industry in the form of respiratory distress and 25% weight loss in broiler chickens. This study discusses the administration of Avian Influenza (AI) H9N2 strain inactivated vaccine to commercial broiler chickens at JAPFA COMFEED Myanmar Pte Ltd. The vaccination process was carried out through subcutaneous injection in the neck and intramuscular injection in the pectoralis musculus in the chest area of chickens at 11 days of age, with a dose of 0.5 ml per chicken. The vaccine used was VAKSIMUNE® AI H9 which uses oil as the emulsion material. Results from the HI serology test showed that 6 cages from Myothar and Wah Net Chaung farms showed protective antibody titers against Avian Influenza H9N2 virus at 30-40 days of age, with normal titer values or ≥24. This indicates that the AI subtype H9N2vaccine used is an effective antigen, capable of stimulating the broiler immune system, and has implications for increasing antibodies every week. This study contributes to the understanding of AI H9N2 vaccination management in commercial broilers at JAPFA COMFEED Myanmar Pte Ltd. With positive results in the serology test, it can be concluded that vaccination using VAKSIMUNE® AI H9 is effective in protecting broilers from Avian Influenza H9N2 infection, providing strong support to the sustainability of production and animal health in a commercial farm environment.
PKM PENERAPAN RECIRCULATING AQUACULTURE SYSTEM UNTUK PENGELOLAAN AIR DAN KESEHATAN IKAN BERKELANJUTAN DI POKDAKAN ROI LELE KABUPATEN MALANG Yanuhar, Uun; Wuragil, Dyah Kinasih; Suryanto, Heru; Mufidah, Rhobithotus; Fauliza, Tifa; Wardani, Nezya Pramudya; Pahlefi, Reval; Adhitya, Dewa Sukma Trinanda; Caesar, Nico Rahman
Jurnal Pengabdian Pendidikan dan Teknologi (JP2T) Vol 3, No 2 (2022)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um080v3i22022p159-165

Abstract

Pemeliharaan ikan lele yang dilakukan pembudidaya di Pokdakan ROI Lele sebagian besar tidak melakukan upaya pengelolaan kualitas air seperti, filtrasi, sirkulasi maupun penggantian air (water exchange). Hal ini menyebabkan penurunan mutu air sebagai akibat dari penumpukan sisa pakan maupun feses ikan serta sisa hasil metabolisme lainnya. Lingkungan perairan merupakan suatu hal yang sangat berperan penting dalam pertumbuhan ikan lele (Clarias sp.). Oleh karena itu, perlu adanya manajemen lingkungan dan kesehatan pada pemeliharaan ikan lele (Clarias sp.). Keberhasilan suatu usaha budidaya sangat erat kaitannya dengan kondisi lingkungan yang optimum untuk kelangsungan hidup dan pertumbuhan ikan yang dipelihara. Setelah Tim PKM Mengidentifikasi pemecahan masalah yang ada, maka kegiatan pemecahan atau pengembangan yang dilakukan harus sesuai dengan kebutuhan yakni dengan menerapkan teknologi Recirculating Aquaculture System (RAS) pada kolam budidaya ikan di Pokdakan ROI Lele.  Dalam penerapan sistem RAS, penggunaan filtrasi air sangat menentukan keberhasilan sirkulasi air. Pada progam PKM ini akan menggunakan Filtrasi air terdiri dari filter mekanis, filter biologi dan filter kimia. Hasil dari kegiatan pengabdian masyarakat PKM terhadap mitra adalah Pengetahuannya meningkat, keterampilannya meningkat, kapasitas produksi meningkat dan keuntungannya meningkat.
Betatrophin: A promising biomarker for metabolic syndrome and diabetes mellitus risk screening in teenagers Susanto, Hendra; Aulanni’am, Aulanni’am; Wuragil, Dyah Kinasih; Taufiq, Ahmad; Sunaryono, Sunaryono; Trijoyo Purnomo, Jerry Dwi; Krisnawati, Dyah Ika; Sholeh, Moch
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6028

Abstract

Metabolic syndrome (MetS) and diabetes mellitus (DM) have become primary concerns worldwide, especially among the younger population. The Indonesian Boarding School model (IBS/Boarding School) is a large education system with a significant number of pupils (teenagers) and has the potential to become a center for metabolic disease, particularly among teenagers, due to their daily intake. This study aimed to provide a baseline screening for MetS and the risk of DM development in Boarding School teenagers. During this observational study, 90 healthy serological samples were obtained from senior and junior high school students. The circulating level of betatrophin was measured using a human betatrophin ELISA kit. Additionally, metabolic syndrome and DM screening data were analyzed using a rapid reverse-flow immunochromatography kit for 65 kDa glutamic acid decarboxylase (GAD65). Among the 90 healthy students, a high prevalence of GAD65 was observed, indicating a potential risk factor for metabolic diseases. Furthermore, higher serum betatrophin levels were observed in the samples. The circulating level of betatrophin was found to have a significant correlation with age, gender, body mass index (BMI), systolic blood pressure (SBP), fasting blood glucose (FBG), sleeping duration, and duration of stay at Boarding School (p < 0.05). Betatrophin emerged as a potential predictor of BMI, SBP, and FBG in students (p < 0.05). Both betatrophin and GAD65 have shown promise as future biomarkers, opening up a new avenue for assessing metabolic syndrome and the risk of DM. This underscores the importance of future programs in Boarding Schools focusing on MetS and DM prevention management, making the audience feel the significance of their work in addressing these pressing health issues.
Co-Authors Adhitya, Dewa Sukma Trinanda Adrenalin, Sruti Listra Ahmad Taufiq Aulanni'am, Aulanni'am Aulanni’am, Aulanni’am Cahyo, Budi Nur Chanif Mahdi Dewi, Made Artari Dirgahariyawan, Tri Cahyo Dodik Prasetyo Fauliza, Tifa Hendra Susanto Herawati Herawati Heru Suryanto Julianto, Andri Krisnawati, Dyah Ika Mufidah, Rhobithotus Nico Rahman Caesar Pahlefi, Reval sholeh, moch Sunaryono Sunaryono, Sunaryono Trijoyo Purnomo, Jerry Dwi Verian, Bagas Ridjalu Wardani, Nezya Pramudya Yanuhar, Uun