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Journal : JURNAL AGROTEKNOLOGI

ANALISIS KEKERABATAN GENETIK TANAMAN PADI (Oryza sativa L.) DI KABUPATEN KAMPAR DENGAN MENGGUNAKAN PENANDA Random Amplified Polymorphic DNA (RAPD) Rita Elfianis; Joko Warino; Rosmaina Rosmaina; Suherman Suherman; Zulfahmi Zulfahmi
Jurnal Agroteknologi Vol 11, No 2 (2021): Februari 2021
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v11i2.10013

Abstract

Rice is a cereal crop that belongs to the graminae family of high economic value, and is the main food for more than a portion of the world's population. This study aims to determine the relationship genetic of rice in Kampar by RAPD marker. Eight genotypes of rice from district in Kampar were amplified using eight primers (OPA 5, OPB 7, OPC 19, OPD 2, OPD 3, OPD 8, OPD 11, and OPD 13). The analysis of molecular data was performed by using NTSys pc -2.02 and GenAlex 6.1 . The results of the amplification of rice produced 48 loci with DNA band sizes obtained ranging from 350-1700 bp. The average percentage of polymorphic loci of the eight primers was 49.80%, where the highest percentage of polymorphic loci produced by OPA-7 primer was 75%, while the lowest polymorphic locus percentage was observed in OPD-13 primer which was 14.28%. The value of genetic distance between rice from Kampar in this study is in the range of 0.06 - 0.37. At a genetic distance of 0.79, rice plants can be grouped into two groups, namely the first group consisting of sokan and coku. The second group consisted of suntiong, korean , cupak putio, kuniong, jangguik, and cupak tenggi. The results of this study are expected to be taken into consideration in developing rice breeding strategies in the future.
KERAGAMAN GENETIK MUTAN M-2 CABAI MERAH KERITING (Capsicum annuum L.) BERDASARKAN PENANDA RAPD Rosmaina Rosmaina; Dedi Mulyadi; Rita Elfianis; Zulfahmi Zulfahmi
Jurnal Agroteknologi Vol 10, No 2 (2020): Februari 2020
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v10i2.8780

Abstract

Chili is an important horticultural plant in Indonesia. This research aims to carry out RAPD analysis on Mutant M2 of chili pepper (Capsicum annuum L.). Six M2 genotypes of chili irradiated by gamma ray and control plants were amplified by 16 random primers. The amplification results of M2 chili with 16 primers produced 118 loci, with fragment sizes ranging from 150-2000 bp. The number of polymorphic loci was 96 loci and the percentage of polymorphic loci was 83.23%. The DNA fragment polymorphism produced in this research was relatively high and it showed that the gamma ray mutagen applied produced high chili genetic diversity. The value of genetic similarity between control plants and mutant plants ranged from 0.7474 to 0.4874. UPGMA dendogram classified seven genotypes tested into three groups, the first group consisted of mutants R2U6 and R2U17, the second group was mutants R1U14 and R1U17, and the third group was mutants R2U8, mutants R2U2 and control plants. The finding of this research can be used as a basic selection of genetic material for chili’s breeding in the future.
PENGARUH SKARIFIKASI DAN HORMON GIBERELIN (GA3) TERHADAP DAYA KECAMBAH DAN PERTUMBUHAN BIBIT PALEM PUTRI (Veitchia merillii) Rita Elfianis; Siti Hartina; Indah Permanasari; Jully Handoko
Jurnal Agroteknologi Vol 10, No 1 (2019): Agustus 2019
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v10i1.7306

Abstract

Christmas palm is a plant that has high economic value as an ornamental plant and is in great demand. Christmas palm seeds begin to germinate 3-4 weeks after planting, slow germination because of to seeds experiencing physical dormancy. To break dormancy can be done by scarification and immersion GA3. The purpose of this study was to determine the effect of scarification and GA3 on the germination and growth of christmas palm seedlings and the interaction between the two treatments. This research was carried out from December 2017 to March 2018 on experimental Field and Agronomy Laboratory Faculty of Agriculture and Animal Science State Islamic University Sultan Syarif Kasim Riau. The method used Randomized Complete Block Design (RCBD) with two factors and four replications. The first factor was the scarification (control and with scarification) and the second factor is immersion GA3 (0 ppm, 150 ppm, 300 ppm and 450 ppm). The results showed that scarification by means of sandpaper can increase the growth rate and height of the christmas palm plant.The dipping of GA3 with concentration of 450 ppm for 2 hours is the best concentration for growth speed. There was no interaction between scarification and GA3 immersion on all observational parameters both in germination and in the nursery of chritmas palm plants on the land.
PENGARUH KONSENTRASI DAN LAMA PERENDAMAN ASAM SULFAT TERHADAP PERKECAMBAHAN BENIH DELIMA MERAH (Punica granatum L.) Rita Elfianis; Nadia Putri; Syukria Ikhsan Zam
Jurnal Agroteknologi Vol 14, No 1 (2023): Agustus 2023
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v14i1.17481

Abstract

The problem of propagation of Punica granatum, was hard seed, and it difficult to germinate. One of the efforts to solve the problem is using H2SO4 as to breakdown of the hard seed. This study aimed to obtain the best concentration of H2SO4 and soaking time, as well as the interaction between H2SO4 concentration and soaking time on the germination of  Punica granatum seeds. This study used a completely randomized design (CRD) consisting of 2 factors. The first factor is H2SO4 concentration (0%, 20%, 40%, 60%, and 80%), and the second factor is soaking time (10, 15, and 20 minutes). Parameters observed were germination, germination rate, vigor index, germination height, root length, weight of sprouts, and dry weight of Punica granatum sprouts. The results showed the use of water was the best treatment on germination (85.30%), germination rate (17.37 days), vigor index (2.24%), germination height (7.80 cm), and root length of Punica granatum (7.05 cm), soaking time did not effect the germination of Punica granatum, and the interaction between the use of water and the soaking time 15 minutes was the best treatment on the parameters of fresh weight of sprouts (0.17 g) and dry weight of Punica granatum sprouts (0.13 g).