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All Journal Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Abdi Insani Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Journal Of Sustainable Research In Management Of Agroindustry (SURIMI) Journal of Agriculture (JoA) Agroista: Jurnal Agroteknologi
Himawan, Achmad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Nursing

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Identifikasi Jamur pada Akar Tanaman Karet yang Berasal dari KP 2 (SEAT) Ungaran Irfani, Hidayat; Himawan, Achmad; Kristalisasi, Elizabeth Nanik
AGROISTA : Jurnal Agroteknologi Vol. 9 No. 2 (2025): NOVEMBER
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v9i2.1653

Abstract

Fungi can grow and live in plant tissue. Endophytic fungi can provide benefits to plants in the form of increased resistance to disease attacks and can stimulate growth. This research aims to isolate and identify fungi in the roots of rubber plants in KP2 (SEAT) Ungaran. Sampling was taken at the KP2 (SEAT) Ungaran rubber plantation, followed by isolation and identification at the Laboratory of the Institute of Agriculture STIPER Yogyakarta. The research started from April to June 2024. Sampling used a diagonal method and the fungal identification method used a descriptive method. The results of isolation and identification obtained 13 isolates identified as the genus Colletotrichum sp. (B1P5U1 and B3P2U4), Fusarium sp. (B1P5U2, B1P5U3, B2P2U3 and B3P3U3), Geotrichum sp. (B2P5U1), Paecilomyces sp. (B3P1U5), Penicillium sp. (B1P1U3, B1P4U5, and B3P2U2), Rhizoctonia sp. (B2P4U3), Rhizopus sp. (B1P1U4), and 2 unknown isolates (B2P4U2 and B2P5U2).
Analysis of Genetic Variation of Ganoderma sp. as an Initial Step in Controlling Palm oil Basal Stem Rot Disease Himawan, Achmad
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 7 No. 3 (2025): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 7 No. 3 September 2025
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v7i3.4940

Abstract

Basal stem rot (BSR) disease caused by Ganoderma spp., particularly Ganoderma boninense, represents a major threat to the sustainability of Indonesia's oil palm industry, resulting in productivity losses of 50-80%. High genetic variation among pathogens constitutes a primary obstacle in developing effective control strategies. This study aimed to analyze the genetic variation in Ganoderma as a preliminary step toward controlling basal stem rot in oil palm using ITS region DNA sequencing with ITS1 and ITS4 primers. Three Ganoderma sp. isolates were used: code 1 from the Biotechnology Laboratory of Lampung University, code 2 from the Plant Pest and Disease Laboratory of Gadjah Mada University, and code 3 from an online store. DNA extraction was performed using the TIANGEN Plant Genomic DNA Kit; PCR amplification with ITS1-ITS4 primers; and the PCR products were sequenced and analyzed using BioEdit and NCBI BLAST. Results showed that isolates code 1 and code 2 were identified as Ganoderma boninense, while isolate code 3 was contaminated with Fusarium sp. Alignment analysis using BioEdit confirmed extremely high genetic variation between the two G. boninense isolates with extensive nucleotide polymorphisms. This significant intraspecific diversity indicates the complexity of basal stem rot pathogen populations and emphasizes the importance of molecular approaches for accurate characterization. These findings provide a scientific foundation for developing more effective and adaptive control strategies and support oil palm resistance breeding programs based on representative pathogen genetic characteristics.
Co-Authors Elisabeth Nanik Kristalisasi Falah, M. Darul Herlambang, Hari Inti Irfani, Hidayat Jaya, Galang Indra Kristalisasi, E. Nanik Kristalisasi, Elizabeth Nanik Kusmawanto, Arif Mawandha, Hangger Gahara Putra, Heru Admadja Reza Widyasaputra Rochmiyati, Sri Manu ryan, Ryan Firman Syah Sinurat, Azwaer Tri Ivani Sri Suryanti Suryansyah, Mhd. Rizky Susanto, Hendra Ardi Syah, Ryan Firman Uktoro, Arief Ika