Nandariyah, Nandariyah
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Hubungan Kekerabatan Genetik Rambutan (Nephelium lappaceum L.) Berdasarkan Lima Marka RAPD (Random Amplified Polimorphic DNA) Fitriyanti, Rafika Indah; Yuniastuti, Endang; Nandariyah, Nandariyah
Agrikultura Vol 34, No 2 (2023): Agustus, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i2.41258

Abstract

Rambutan (Nephelium lappaceum L.) merupakan salah satu tanaman tropis Indonesia yang mempunyai banyak potensi ekonomi sebagai upaya diversitas buah-buahan. Pelestarian plasma nutfah rambutan perlu dilakukan lebih lanjut baik secara kualitas maupun kuantitas. Metode RAPD digunakan untuk memperoleh informasi mengenai keragaman genetik antar jenis tanaman rambutan. Penelitian dilakukan menggunakan metode RAPD (Random Amplified Polymorphic DNA) dengan 20 primer. Hasil penelitian menunjukkan bahwa seleksi 20 primer menghasilkan 5 primer terbaik yaitu (OPD 2, OPD 5, OPD 6, OPD 10, OPD 12), pada ukuran 50-2000 bp dengan total 48 pita (42 pita polimorfik dan 6 pita monomorfik). Tingkat polimorfisme keragaman antar jenis rambutan sebesar 86,24%. Hasil uji dendogram UPGMA menunjukkan adanya keragaman genetik yang tergolong tinggi pada rambutan. Terbentuk dua klaster utama dengan tingkat kesamaan antara 56-77%. Rambutan jenis Rapiah berkerabat dekat dengan Sibatuk Ganal sebesar 77%.
Comparative Effects of Organic and Inorganic Fertilization with Mycorrhizal Inoculation on the Growth and P-Uptake of Salak (Salacca zalacca) Seedlings in a Compost-Amended Andisol Anasrullah, Anasrullah; Nandariyah, Nandariyah; Hartati, Sri
Jurnal Agroteknologi (Agronu) Vol 5 No 01 (2026): Jurnal Agroteknologi (In Progress)
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53863/agronu.v5i01.1796

Abstract

The cultivation of salak (Salacca zalacca) on Andisols is constrained by low phosphorus (P) availability due to the soil's high P-fixation capacity. To evaluate strategies for enhancing P uptake, a 180-day greenhouse experiment was conducted using a factorial completely randomized design. Salak seedlings were grown in a compost-amended Andisol (2:1 v/v) and subjected to three fertilizer treatments (control [P0], liquid organic fertilizer [P1], and inorganic NPK [P2]) combined with three dosages of a Glomus-based arbuscular mycorrhizal fungi (AMF) inoculum (0 g [M0], 30 g [M1], and 60 g [M2] polybag⁻¹). The results revealed distinct treatment effects on soil, microbial, and plant parameters. In the soil, the inorganic NPK fertilizer (P2) induced significant acidification (pH 6.17), while the liquid organic fertilizer (P1) maintained a more neutral pH (7.03); however, final soil-available P was not significantly different among treatments. Biologically, the P2 treatment severely suppressed the mean mycorrhizal infection rate to 20.0%, significantly lower than the 53.3% observed under the P1 treatment. These effects directly translated to plant growth, where P1 produced significantly taller plants and greater fresh weight, and the P1M2 combination yielded the longest roots (42.43 cm). Ultimately, these improvements culminated in superior plant nutrition, with the P1 treatment facilitating the highest P uptake (0.145 g/plant), nearly double that of the P2 treatment (0.073 g/plant). The findings systematically demonstrate that an integrated approach using liquid organic fertilizer and AMF inoculation is a superior strategy for enhancing nutrient uptake efficiency and promoting vigorous growth in salak nurseries on P-fixing Andisols
Performance and Selection of Mutant Black Rice with High Lignin Handayani, Rizky; Nandariyah, Nandariyah; Parjanto, Parjanto; Riyatun, Riyatun; Sutarno, Sutarno
Agro Bali : Agricultural Journal Vol 7, No 3 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i3.1927

Abstract

Eating black rice can avoid the risk, reduce insulin in the body, bind free radicals, and reduce weight. However, black rice still has disadvantages, like long life, tall stems, less resistance to pests and diseases, and low yields. Activities were carried out from August 2023 to November 2023 in Pakahan Village, Jogonalan, Klaten. The planting materials used were selected M3 mutant black rice seeds irradiated with 200 Gy gamma rays and Cempo Ireng black rice as a control plant. Planting used a randomized complete block design without replication with the pedigree selection method. The parameters measured were harvest age, plant height, productive tillers, and seed weight per plant. Descriptive analysis was used and a 5% level T test was carried out to compare the characteristics of the M4 genotype with the control (without irradiation). The research results showed that 12 individuals containing high lignin were selected which could be further developed for the next generation.