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All Journal Agrikultura Agrosains: Jurnal Penelitian Agronomi Caraka Tani: Journal of Sustainable Agriculture Agro Bali: Agricultural Journal SEMAR Jurnal Agroteknologi Journal of Biodiversity and Biotechnology
Nandariyah, Nandariyah
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Agriculture, Biological Sciences & Forestry Humanities Education Environmental Science Languange, Linguistic, Communication & Media Social Sciences

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Hubungan Kekerabatan Genetik Rambutan (Nephelium lappaceum L.) Berdasarkan Lima Marka RAPD (Random Amplified Polimorphic DNA) Fitriyanti, Rafika Indah; Yuniastuti, Endang; Nandariyah, Nandariyah
Agrikultura Vol 34, No 2 (2023): Agustus, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i2.41258

Abstract

Rambutan (Nephelium lappaceum L.) merupakan salah satu tanaman tropis Indonesia yang mempunyai banyak potensi ekonomi sebagai upaya diversitas buah-buahan. Pelestarian plasma nutfah rambutan perlu dilakukan lebih lanjut baik secara kualitas maupun kuantitas. Metode RAPD digunakan untuk memperoleh informasi mengenai keragaman genetik antar jenis tanaman rambutan. Penelitian dilakukan menggunakan metode RAPD (Random Amplified Polymorphic DNA) dengan 20 primer. Hasil penelitian menunjukkan bahwa seleksi 20 primer menghasilkan 5 primer terbaik yaitu (OPD 2, OPD 5, OPD 6, OPD 10, OPD 12), pada ukuran 50-2000 bp dengan total 48 pita (42 pita polimorfik dan 6 pita monomorfik). Tingkat polimorfisme keragaman antar jenis rambutan sebesar 86,24%. Hasil uji dendogram UPGMA menunjukkan adanya keragaman genetik yang tergolong tinggi pada rambutan. Terbentuk dua klaster utama dengan tingkat kesamaan antara 56-77%. Rambutan jenis Rapiah berkerabat dekat dengan Sibatuk Ganal sebesar 77%.
Comparative Effects of Organic and Inorganic Fertilization with Mycorrhizal Inoculation on the Growth and P-Uptake of Salak (Salacca zalacca) Seedlings in a Compost-Amended Andisol Anasrullah, Anasrullah; Nandariyah, Nandariyah; Hartati, Sri
Jurnal Agroteknologi (Agronu) Vol 5 No 01 (2026): Jurnal Agroteknologi
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53863/agronu.v5i01.1796

Abstract

The cultivation of salak (Salacca zalacca) on Andisols is constrained by low phosphorus (P) availability due to the soil's high P-fixation capacity. To evaluate strategies for enhancing P uptake, a 180-day greenhouse experiment was conducted using a factorial completely randomized design. Salak seedlings were grown in a compost-amended Andisol (2:1 v/v) and subjected to three fertilizer treatments (control [P0], liquid organic fertilizer [P1], and inorganic NPK [P2]) combined with three dosages of a Glomus-based arbuscular mycorrhizal fungi (AMF) inoculum (0 g [M0], 30 g [M1], and 60 g [M2] polybag⁻¹). The results revealed distinct treatment effects on soil, microbial, and plant parameters. In the soil, the inorganic NPK fertilizer (P2) induced significant acidification (pH 6.17), while the liquid organic fertilizer (P1) maintained a more neutral pH (7.03); however, final soil-available P was not significantly different among treatments. Biologically, the P2 treatment severely suppressed the mean mycorrhizal infection rate to 20.0%, significantly lower than the 53.3% observed under the P1 treatment. These effects directly translated to plant growth, where P1 produced significantly taller plants and greater fresh weight, and the P1M2 combination yielded the longest roots (42.43 cm). Ultimately, these improvements culminated in superior plant nutrition, with the P1 treatment facilitating the highest P uptake (0.145 g/plant), nearly double that of the P2 treatment (0.073 g/plant). The findings systematically demonstrate that an integrated approach using liquid organic fertilizer and AMF inoculation is a superior strategy for enhancing nutrient uptake efficiency and promoting vigorous growth in salak nurseries on P-fixing Andisols
Performance and Selection of Mutant Black Rice with High Lignin Handayani, Rizky; Nandariyah, Nandariyah; Parjanto, Parjanto; Riyatun, Riyatun; Sutarno, Sutarno
Agro Bali : Agricultural Journal Vol 7, No 3 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i3.1927

Abstract

Eating black rice can avoid the risk, reduce insulin in the body, bind free radicals, and reduce weight. However, black rice still has disadvantages, like long life, tall stems, less resistance to pests and diseases, and low yields. Activities were carried out from August 2023 to November 2023 in Pakahan Village, Jogonalan, Klaten. The planting materials used were selected M3 mutant black rice seeds irradiated with 200 Gy gamma rays and Cempo Ireng black rice as a control plant. Planting used a randomized complete block design without replication with the pedigree selection method. The parameters measured were harvest age, plant height, productive tillers, and seed weight per plant. Descriptive analysis was used and a 5% level T test was carried out to compare the characteristics of the M4 genotype with the control (without irradiation). The research results showed that 12 individuals containing high lignin were selected which could be further developed for the next generation. 
Karakterisasi Morfologi Galur Harapan M0 dan M3 Padi Hitam Hasil Iradiasi Sinar Gamma Nandariyah, Nandariyah; Prastyaningrum, Suci; Manurung, Ida Rumia; Sutarno, Sutarno; Riyatun, Riyatun
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.90640

Abstract

Cempo Ireng is a black rice from Sleman that has a long harvesting period, high posture, and low productivity. Efforts to overcome these problems are mutation induction with gamma irradiation and have produced potential lines (8, 51, and 52) with early maturing, plant height 140,26 cm, and productivity 4,71 tons/ha, but not yet resistant to brown planthopper (BPH). This research is a follow-up study of 200 Gy gamma irradiation treatment of three potential lines to improve BPH resistance traits and increase genetic diversity. This study aims to determine changes in morphological characteristics and obtain superior individuals. The research was conducted in paddy field of Pakahan Village, Jogonalan District, Klaten Regency in February - July 2022 using 8:1 jajar legowo system. Observations of 25 morphological characters were carried out on 30 plants of each lines and were based on the Rice Plant Characterization and Evaluation (2003). Data analyzed by descriptively and qualitatively using Chi Square test with table value at 5% significance. The results showed there were changes in 17 morphological characters in the M3 population of black rice from gamma irradiation and individual selection resulted in 12 mutant plants.
Karakterisasi Morfologi Tetua dan Hybrid Anggrek Dendrobium bigibbum dan Dendrobium lineale Hartati, Sri; Nandariyah, Nandariyah; Muliawati, Endang Setia; Sukaya, Sukaya; Yuniastuti, Endang; Parjanto, Parjanto; Manurung, Ida Rumia
Agrosains : Jurnal Penelitian Agronomi Vol 24, No 2 (2022): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v24i2.65255

Abstract

Dendrobium are one of the most cultivated ornamental orchids plants. However, orchids as genetic resources have not been optimally utilized as parents in crossing to produce offspring according to the characteristics desired by consumers. This study aims to study the similarities between the Hybrid results of crosses compared to their parent orchids and obtain a new variety of Hybrid from crosses of Dendrobium bigibbum and Dendrobium lineale. This research was conducted at the Bogor Botanical Gardens and Plosorejo Village, Matesih District, Karanganyar Regency, Central Java. The materials used were 14 samples of orchid plants, including three examples of D. bigibbum orchids, three samples of D. lineale orchids, four samples of Hybrid plants resulting from crosses of ♀ D. bigibbum X ♂ D. lineale, and four samples of Hybrid plants from crossing ♀ D. lineale X ♂ D. bigibbum. Observation variables include quantitative variables. The quantitative morphological variables use the guideline developed by BALITHI. To analyze the data using the program of NTSYSpc 2.02i version. The results showed that based on quantitative observations, Dendrobium lineale as a female parent can change the size of the dorsal sepal width, flower arrangement length, lateral sepal length, and flower length. And Dendrobium bigibbum as the female parent can change the size of the petal length, lateral sepal width, flower width, pseudobulb thickness, pseudobulb length, leaf length, and leaf width.
Keragaman Genetik Sirih Cina (Peperomia pellucida L.) Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA) Nandariyah, Nandariyah; Ningsih, Hardian; Fadhillah, Annisa Nur; Parjanto, Parjanto; Paryanto, Edi
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 1 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i1.92694

Abstract

Chinese betel is a plant widely found in Indonesia, especially in the ex-Karasidenan area of Surakarta. The existence of growing Chinese betel can be found at various altitudes with humid growing conditions with low sun intensity. People in Surakarta believe that Chinese betel can be used as a traditional medicine. Chinese betel nut is suspected to contain a minerals and bioactive substances that are beneficial to health. Currently, there are few references to Chinese betel, especially related to genetic diversity. This study aims to study the diversity of Chinese betel band patterns based on RAPD markers using OPA-3, OPA-4, OPA-11, OPC-05, and OPD-4 primers. This research was carried out at the Plant Breeding Laboratory, Faculty of Agriculture, Gadjah Mada University, Yogyakarta in July - November 2023 using Chinese betel leaf DNA taken from various places in the ex-karasidenan area of Surakarta. The results of the data analyzed by NTSYSpc version 2.02 with the SimQual (Similarity for Qualitative Data) function using the UPGMA (Unweighted Pair Group with Arithmetic Averaging) method based on the Jaccard similarity index value. The amplification results showed 30 bands with 13 polymorphic bands and 17 monomorphic bands. The level of polymorphism among Chinese betel samples in the five RAPD primers used presented 44.36% of the total 13 polymorphic bands. Jaccard's genetic similarity matrix data ranged from 0.667 – 0.964. The results of the dendogram show that the sample is divided into two clusters with a diversity index of 0.28.
Study of Black Rice Parents Performance and the Crossing Ability Nandariyah, Nandariyah; Sukaya, Sukaya; Purnomo, Djoko; Sutarno, Sutarno; Yuniastuti, Endang; Az-Zahra, Catalina Dara Ayu
Caraka Tani: Journal of Sustainable Agriculture Vol 38, No 1 (2023): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v38i1.60245

Abstract

Black rice generally has problems in cultivation such as relatively long plant life, high habitus and low productivity. Plant breeders use several methods in hybridization activities, such as backcross method that can lead to the development towards emphasizing the superiority of each parent. This research aimed to study and determined the success rate of the black rice lines F1 backcross and the parents’ performance. This research was conducted using F1 of promising lines, parental lines and the Jeliteng variety with 9 sets of crosses. Each line and variety were repeated 3 times, in total there were 57 experimental units. The observation parameters of this research were plant height, crossing success, weight of seeds, length and width of grain, flowering age, harvesting age also number of grains and unfilled spikelet. The results of this study showed that parents plant height was positively correlated with the number of productive tillers. The taller the plant, the more productive tillers and the more flowers can be crossed. The success of the cross can increase with the number of flowers crossed. The success of crosses between F1 black rice promising lines and their parents has a success rate range of 10.82% to 33.75%. The findings imply that F1 crossbreeding of black rice promising lines with their parents can be carried out to produce backcross offspring.
Diversifikasi Tanaman Pekarangan Dengan Tanaman Alpukat Untuk Meningkatkan Gizi Keluarga Hartati, Sri; Yunus, Ahmad; Nandariyah, Nandariyah; Yuniastuti, Endang; Pujiasmanto, Bambang; Purwanto, Edi; Samanhudi, Samanhudi; Sulandjari, Sulandjari; Ratriyanto, Adi; Prastowo, Sigit; Manurung, Ida Rumia; Suryanti, Venty; Susilowati, Ari; Artanti, Anif Nur; Mulyani, Sri; Dirgahayu, Paramasari
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 11, No 2 (2022): November
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v11i2.61199

Abstract

Alpukat (Persea americana) merupakan buah yang sudah sangat dikenal dan digemari oleh masyarakat. Alpukat diketahui memiliki khasiat sebagai antioksidan, antidiabetik dan efek hipolipidemik. Nutrisi yang terkandung dalam buah alpukat mampu membantu memenuhi kebutuhan nutrisi harian tubuh. Namun, cara budidaya tanaman Alpukat  sangat berpengaruh pada khasiat atau kualitas dan kuantitas dari produk yang dihasilkan. Sehingga diperlukan pemahaman tentang cara budidaya tanaman Alpukat kepada masyarakat. Tujuan yang ingin dicapai dalam kegiatan ini adalah agar masyarakat lebih memahami cara budidaya tanaman alpukat sebagai tanaman pekarangan dan mengetahui manfaat buah Alpukat dalam meningkatkan gizi keluarga. Kegiatan pengabdian sudah dilaksanakan dengan metode penyuluhan dengan penyampaian materi tentang budidaya tanaman Alpukat, manfaat Alpukat, serta berbagai olahan Alpukat sedangkan pada akhir sesi dilakukan pemberian bibit Alpukat serta pupuk pada masing-masing peserta. Kegiatan yang sudah terlaksana ini bermanfaat untuk menambah pengetahuan dan keterampilan masyarakat khususnya masyarakat Kelurahan Wonokarto tentang budidaya tanaman Alpukat dan keanekaragaman manfaat Alpukat untuk kesehatan.Kata Kunci: alpukat; budidaya; penyuluhan; pupuk
Growth Orchid (♀) Dendrobium Liniale x (♂) Dendrobium Biggibum on Medium NAA and Organic Material in Vitro Hartati, Sri; Purwanti, Heni; Nandariyah, Nandariyah
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.60092

Abstract

The research aimed to study the influence of the application of NAA concentration, the addition of organic materials and a combination of both on media Knudson C towards the growth of orchid plantlet (♀) Dendrobium liniale >< (♂) Dendrobium biggibum. The purpose of using media Knudson C in culture in vitro is to optimize the growth of orchid plantlet Dendrobium liniale and Dendrobium biggibum. The experiments were carried out in the Laboratory of Plant Physiology and Biotechnology Faculty of Agriculture, Sebelas Maret University Surakarta. The research used Complete Randomized Design (CRD) with 2 treatment factors, factor I was the concentration of NAA (0 ppm, 1 ppm, 3 ppm and 5 ppm); and factor II was the organic materials (coconut water, banana, potatoes and sweet potatoes). In total there were 20 treatment combinations . Each combination was repeated four times. The results showed that the combination of sweet potato and NAA 5 ppm produced the following characteristics: the fastest root growth, most root length, and the largest number of leaves; while the combination of banana extracts and NAA 5 ppm produced a number of roots, the shoots appear most rapidly, and the highest number of shootsKeywords: genetic diversity; tissue culture; medium;
Co-Authors Adi Ratriyanto Anasrullah, Anasrullah Anif Nur Artanti, Anif Nur ARI SUSILOWATI Az-Zahra, Catalina Dara Ayu Bambang Pujiasmanto Djoko Purnomo Edi Purwanto Endang Setia Muliawati Endang Yuniastuti Fadhillah, Annisa Nur Fitriyanti, Rafika Indah Handayani, Rizky Hardian Ningsih, Hardian Ida Rumia Manurung Paramasari Dirgahayu Parjanto Parjanto Paryanto, Edi Prastyaningrum, Suci Purwanti, Heni Riyatun, Riyatun Samanhudi Samanhudi Sigit PRASTOWO Sri Hartati Sri Mulyani Sukaya, Sukaya Sulandjari Sulandjari, Sulandjari Sutarno Sutarno Venty Suryanti Yunus, Ahmad