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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Jurnal Teknologi Laboratorium Biosaintifika: Journal of Biology & Biology Education Prosiding University Research Colloquium Genbinesia Journal of Biology
Prastiyanto, Muhammad Evy
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Other

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Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria Prastiyanto, Muhammad Evy; Wardoyo, Fandhi Adi; Wilson, Wildiani; Darmawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria
In-vitro antibacterial activity of the seed extract of three member Artocarpus towards methicillin resistant Staphylococcus aureus (MRSA) Prastiyanto, Muhammad Evy; Azizah, Inas Hasna; Haqi, Hafizha Dara; Yulianto, Bagus Dwi; Agmala, Aulia Bella; Radipasari, Zhafira Dias; Astuti, Nita Ayu Dwi; Putri, Arifani Rahma
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): (In Progress)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.237

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from biological sources. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method (Minimum inhibitory concentration [MIC] and Minimum bactericidal concentration [MBC]). The best antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. The data have revealed that all seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Further in vivo research and discovery of mode action are needed to shed light on their antibacterial effects. Research on anti-MRSA of seed extract of Artocarpus against MRSA has not been reported. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.
Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body Prastiyanto, Muhammad Evy; darmwati, Sri; iswara, Arya; Setyaningtyas, Agnis; Trisnawati, Laily; Syafira, Anna
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4082

Abstract

Pleurotus ostreatus is an edible mushroom that also has potential as medicinal values. In this study, fruiting body of P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The fruiting body powder of P. ostreatus was extracted using methanol by maceration method. Analysis of this compound was done by using anisaldehid sulfuric acid, Dragendorff reagent, and FeCl3. Using the agar well diffusion technique, the extracts were tested for their antimicrobial activity against Staphylococcus aureus (Gram positive), Enterobacter aerogenes (Gram negative) and Candida albican (yeast). The spot results on TLC using crude extract of P. ostreatus is terpenoids. Zone of inhibition for the various extracts varied between 10.9 - 23.2 mm. Ten miligrams extract exhibit maximum antimicrobial activity against most of the tested pathogens 
Black-Pigmented Marine Pseudomonas aeruginosa Exhibiting Anti-Bacterial Activity against Multidrug-Resistant (MDR) Wound Infection Bacteria Prastiyanto, Muhammad Evy; Darmawati, Sri; Daryono, Budi Setiadi; Retnaningrum, Endah
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.880-890

Abstract

The urgency of multidrug-resistant (MDR) bacterial infections in wounds is a significant concern due to the high prevalence of MDR in healthcare settings. Black pigmented marine bacteria, strain PS1C, were isolated from marine sediment on Awur Beach Jepara, Central Java, Indonesia, and cultured in the laboratory. The aim of this research includes molecular identification of strain PS1C, extracting black pigment from strain PS1C, isolation of MDR bacteria from wounds, and evaluating the antibacterial activity of black pigment from strain PS1C against MDR bacteria isolates of wound infections. We examined the 16S rRNA gene sequences strain PS1C to identify the species. Then, the black pigment from PS1C was extracted using methanol: acetone (7:3) solvent. Antibacterial activity was evaluated against MDR bacteria from wounds with the microdilution method. A black-pigmented bacterium was isolated and identified as Pseudomonas aeruginosa PS1C. We found that the black pigment from P. aeruginosa PS1C can be developed as an antibacterial agent against the MDR bacterial isolate of wounds with MIC and MBC values of 6.25-12.5 mg/ml and 6.25-25 mg/ml, respectively. In conclusion, the study's findings highlight the potential of the extract of black pigment from P. aeruginosa PS1C as an antibacterial agent against wound-causing MDR bacteria and reinforce previous research into P. aeruginosa can be isolated from marine sources. Additional in vivo investigations and the identification of the antibacterial activity's mode of action are required.
Phytofluene from Physalis peruviana as promising anti-TB via InhA of Mycobacterium tuberculosis target: an in silico research Prastiyanto, Muhammad Evy; Aini, Nur Sofiatul; Yuanita Rachmawati
Genbinesia Journal of Biology Vol. 1 No. 2 (2022): March 2022
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v1i2.47

Abstract

Tuberculosis (TB) is an infectious disease after severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in developed countries including Indonesia. Drug resistance becomes major issue worldwide and needs prospective therapeutics development. Plant with medicinal properties including Physalis peruviana is one the promising object to be new anti-TB drug candidate. This study aimed to analyze the inhibitory activity of anti-TB agents from aerial parts of P. peruviana. Ligand and protein samples were obtained from PubChem and RCSB PDB, respectively. The bioactive compounds were evaluated their antibacterial prediction and drug-likeness properties throughout PASS Online and SwissADME webservers. Selected ligands then docked via PyRX and measured the output by binding affinity. Visualization of the best outputs was carried out using BIOVIA Discovery Studio. The result showed that phytofluene had the lowest binding affinity topping the isoniazid as control with -7.2 and -5.1 kcal/mol after targeting enoyl-[acyl-carrier-protein] reductase (InhA) protein of Mycobacterium tuberculosis. This concluded that phytofluene functioned as predictive anti-TB therapeutic candidate. Further in vitro and in vivo studies are needed to validate this outcome.
Virtual screening of phytochemical compounds from Physalis peruviana as perspective anti-schistosomiasis M. Ainul Mahbubillah; Aini, Nur Sofiatul; Prastiyanto, Muhammad Evy
Genbinesia Journal of Biology Vol. 1 No. 3 (2022): July 2022
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v1i3.48

Abstract

Schistosomiasis is a neglected tropical disease (NTD) causing by a Schistosoma japonicum parasite via Oncomelania hupensis snails. Even if the the number of cases decreases significantly, there is still drawbacks of conventional medication. Physalis peruviana is promising plant that rich phytochemicals as prospective drug candidiate of schistosomiasis. This study aimed to virtually screen the inhibitory activity of anti-schistososmiasis agents derived from P. peruviana body portion. Compound data were mined from PubChem and assessed their drug properties and target prediction using SwissADME and PASSOnline. Selected phytochemical compounds were screen the pharmacokinetics and toxicity by admetsar webserver. 1,2-benzenedicarboxylic acid and docosane was final filtered compounds as promising anti-schistosomiasis target. Daily dose arrangement should be confirmed through in vitro and in vivo because of the hepatoxicity characteristics of the compounds. Protein kinases of helminth projected to be next protein target of alternative therapeutics for vital roles in organism. To be concluded, 1,2-benzenedicarboxylic acid and docosane is functioned as anti-schistosomiasis candidates with further validation in different analyses.
3,3-dimethyl-octane from Physalis peruviana as promising anti-DENV via ADMET prediction of pkCSM open webserver Prastiyanto, Muhammad Evy; Solekha, Rofiatun; Rohmah, Laila Ainur; Yuanita Rachmawati; Aini, Nur Sofiatul
Genbinesia Journal of Biology Vol. 3 No. 1 (2023): November 2023
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v3i1.63

Abstract

Dengue is caused by the dengue virus (DENV) and being prevalent in 100 tropical and subtropical countries including Indonesia. This disease is spread by Aedes mosquitoes. There is currently no clinically authorized medicine to treat the dengue fever. Physalis peruviana has ethnomedicine application and noted for its antioxidant activities. This study purpose to investigate the pharmacokinetics or ADMET of anti-DENV from leaf parts of P. peruviana. The phytoconstituents data were gathered from multiple sources. The drug property and ADMET prediction were assessed using pkCSM. Following online screening, 3,3-dimethyl-octane functioned as predictive anti-DENV therapeutic candidate. Further dry and wet lab studies are needed to validate this finding.
Suspect TB di Sekitar Tempat Tinggal Penderita TB Paru di Sambiroto Semarang Darmawati, Sri; Prastiyanto, Muhammad Evy; Listiyanti, Apriliya
Prosiding University Research Colloquium Proceeding of The 7th University Research Colloquium 2018: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Tuberkulosis (TB) paru adalah penyakit infeksi yang disebabkan olehbakteri Mycobacterium tuberculosis, dan suspect TB adalah orangyang dicurigai menderita TB. Orang yang tinggal di sekitarpenderita TB berpotensi menjadi suspect (tersangka) TB. Tujuanpenelitian untuk mengetahui gambaran suspect TB di lingkungansekitar tempat tinggal 3 penderita TB Paru di wilayah Sambirotokota Semarang. Jenis penelitian adalah penelitian deskriptif, sampelsebanyak 30 suspect dari 3 titik tempat tinggal penderita TB ParuPositif, dari setiap titik tempat tinggal penderita diambil 10 orangsuspect yang masuk dalam screening penjaringan dengan kriteriaresponden adalah anggota keluarga yang tinggal serumah, tetanggadekat dan sering berkomunikasi secara langsung, kemudiandilakukan pemeriksaan mikroskopis dari dahaknya yaitu dahaksewaktu, pagi, sewaktu dengan pewarnaan metode Ziehl Nellson.Hasil pemeriksaan menunjukkan jumlah suspect Basil Tahan Asamdengan hasil positif sejumlah 4 sampel (13%) dari total 30 sampel.
Penelitian Pendahuluan: Aktivitas Antibakteri Cold Argon Plasma Jet terhadap Multi Drug Resistance (MDR) Bacteria Prastiyanto, Muhammad Evy; Maulida, Gita Rossa; Salsabila, Nia; Darmawati, Sri; Nasruddin, N
Prosiding University Research Colloquium Proceeding of The 10th University Research Colloquium 2019: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Multi drug resistance (MDR) bacteria telah menjadi masalah kesehatan global diseluruh dunia dikarenakan penggunaan antibiotik yang tidak rasional. Tujuan dari penelitian ini untuk mengevaluasi aktivitas antibakteri cold argon plasma jet terhadap Methichillin Resistant Staphylococcus aureus (MRSA), Carbapenem Resistant Pseudomonas aeruginosa (CRPA), dan Extended Spectrum Betalactamase (ESBL) Escherichia coli. Proses uji antibakteri dilakukan dengan alat cold argon plasma jet pada jarak 1 cm dengan variasi waktu 1, 2, 3, 4, dan 5 menit. Aktivitas antibakteri cold argon plasma jet menunjukkan zona hambat tertinggi pada menit ke 5 CRPA 6,5 mm, ESBL E.coli 6,5 mm, MRSA 4 mm. Kesimpulan: cold argon plasma jet memiliki potensial sebagai agen antibakteri.
Co-Authors Agmala, Aulia Bella Aini, Nur Sofiatul Arya Iswara Astuti, Nita Ayu Dwi Azizah, Inas Hasna BUDI SETIADI DARYONO darmwati, Sri Fandhi Adi Wardoyo Haqi, Hafizha Dara Laila Ainur Rohmah Listiyanti, Apriliya M. Ainul Mahbubillah Mahbubillah, M. Ainul Maulida, Gita Rossa Nasruddin, N Putri, Arifani Rahma Radipasari, Zhafira Dias Retnaningrum, Endah Salsabila, Nia Setyaningtyas, Agnis Solekha, Rofiatun Sri Darmawati Syafira, Anna Trisnawati, Laily Wilson, Wildiani Yuanita Rachmawati Yulianto, Bagus Dwi