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All Journal Journal of Midwifery and Nursing INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Medicra (Journal of Medical Laboratory Science/Technology) Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia
Mamay, Mamay
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Nursing Public Health Social Sciences Other

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Journal : Journal of Midwifery and Nursing

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Utilization ethanol extract of sappan wood as an alternative in escherichia coli staining mamay, mamay
Journal of Midwifery and Nursing Vol. 6 No. 1 (2024): January: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v6i1.4565

Abstract

In life, sappan wood is used as an ingredient in traditional drinks that has antioxidant, antimicrobial and has stable pigments. The content of red pigment, brazilin in sappan wood can be used as a dye. The purpose of this study was to determine the potential of sappan wood ethanol extract as an alternative to safranin in gram staining. Ethanol extract of sappan wood 6% was added with alum mordant to obtain a red pigment. The bacteria used Eschericia col, isolated from water. Staining using ethanol extract of sappan wood was carried out for 30 seconds, 3, 5 and 10 minutes. This stain was compared with safranin. The results showed that staining using sappan wood extract shows clarity of the visual field, shape and good color. Based on the results, the ethanol extract of sappan wood with mordant can be used as an alternative to safranin in the gram-staining.
Minimal inhibitory concentration (MIC) of sodium hypochlorit against bacterial isolate from equipment in the microbiology laboratory Mamay, Mamay; Mar’atiningsih, Lia; Atoilah, Elang M.; Mutia, Sifa Rinda
Journal of Midwifery and Nursing Vol. 6 No. 2 (2024): May: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v6i2.5171

Abstract

Microbiology laboratory equipment is susceptible to bacterial contamination and therefore has the potential to become a medium for the spread of disease and infection. The existence of a less than sterile microbiological analysis process in the STIKes Karsa Husada Garut Microbiology Laboratory causes bacterial contamination of laboratory equipment such as bacterial analysis that does not comply with standards or the use of less than sterile equipment. To control the spread of contaminants, a decontamination process is required using a chemical disinfectant, namely sodium hypochlorite. Measuring the effectiveness of a disinfectant by looking at or can be determined by the Minimum Inhibitory Concentration (MIC). The aim of this research was to determine the effectiveness of sodium hypochlorite disinfectant in inhibiting the growth of microorganisms in bacterial isolates from the STIKes Karsa Husada Garut Microbiology Laboratory equipment. The data analysis used was analyzed descriptively. The results of this research are the Minimum Inhibitory Concentration (MIC) of sodium hypochlorite disinfectant on bacterial isolates from the STIKes Karsa Husada Garut Microbiology Laboratory equipment at a concentration of 4%. So it can be concluded that sodium hypochlorite disinfectant with a concentration of 4% is effective in inhibiting the growth of microorganisms.
Co-Authors Apriyadi, Yadi Astari Nurisani Atoilah, Elang M. Dian Hardianti, Dian Ernawati Ernawati Mar’atiningsih, Lia Mutia, Sifa Rinda Mutmaina, Gina Nafsa Nurahma, Ina Aflaha Nurani Ai Erlinawati, Nurani Ai Erlinawati Pratama, Wisnu Arya Sugiah, Sugiah Yogi Rahman Nugraha