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All Journal Journal of Midwifery and Nursing INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Medicra (Journal of Medical Laboratory Science/Technology) Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia
Mamay, Mamay
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Nursing Public Health Social Sciences Other

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Journal : Jurnal Medika Cendikia (e-Journal)

 1 
Antibacterial Activity of Ecoenzyme from Garut Orange Peel (Citrus nobilis var.chrysocarpha) against Propionibacterium acne Nurani Ai Erlinawati, Nurani Ai Erlinawati; Mamay, Mamay; Yogi Rahman Nugraha, Yogi Rahman Nugraha
Jurnal Medika Cendikia Vol 12 No 01 (2025): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v12i01.323

Abstract

Acne is a skin disorder that often occurs in adolescents. Propionibacterium acnes is a bacteria that causes acne. The use of antibacterial substances is very important to prevent and control this bacterial infection. One of the advances in the use of antibacterials comes from the use of ecoenzyme. The process of managing organic waste with ecoenzyme can be an effective and environmentally friendly waste management strategy. Garut orange with the scientific name Citrus nobilis var. chrysocarpa is a local wisdom fruit. Orange peel which is a waste that increases in quantity when the harvest season arrives can be used in the manufacture of ecoenzyme. This study aims to evaluate the antibacterial ability of ecoenzyme from garut orange peel against Propionibacterium acnes bacteria. The fermentation time for ecoenzyme production varied from 30, 60 and 90 days.. The method for the eco enzyme antibacterial test uses the disc diffusion method. Three variations in eco enzyme concentration used to inhibit the growth of isolated bacteria are 33%, 66% and 100%. Ecoenzyme from Garut orange peel shows antibacterial activity against Propionibacterium acne. Antibacterial activity shows an increase with increasing fermentation time of ecoenzyme
UTILIZATION CRYSTAL VIOLET AS NUCLEI DYE IN HISTOPATHOLOGY OF BREAST CANCER Mamay, Mamay; Apriyadi, Yadi; Nurisani, Astari; Hardianti, Dian
Jurnal Medika Cendikia Vol 10 No 2 (2023): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v10i2.226

Abstract

Microscopic examination of cancer development can be identified through histopathological examination. Hematoxylin–eosin (HE) is the gold standard for staining method. This processing can be obstructed if hematoxylin reagent out of stock in the laboratory. Other reagents are needed that can replace the use of hematoxylin, one of which is crystal violet. This study aims to evaluate the use of crystal violet as a nuclei stain in breast cancer preparations. The research sample consisted of 15 breast cancer preparations taken at the sample bank in the anatomical pathology laboratory of RSUD Dr. Slamet Garut. The results showed that from 15 preparations, it was observed that 4 preparations (27%) stained worth and 11 preparations (73%) stained poorly. Based on the research results, crystal violet crystal violet has a low core coloring ability in breast cancer preparations. From the results of this study, it is recommended to modify the crystal violet reagent by paying attention to the acidity of pH and concentration of crystal violet.
Co-Authors Apriyadi, Yadi Astari Nurisani Atoilah, Elang M. Dian Hardianti, Dian Ernawati Ernawati Mar’atiningsih, Lia Mutia, Sifa Rinda Mutmaina, Gina Nafsa Nurahma, Ina Aflaha Nurani Ai Erlinawati, Nurani Ai Erlinawati Pratama, Wisnu Arya Sugiah, Sugiah Yogi Rahman Nugraha