Darmawan, Jadmiko
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PERFORMANCE OF PERKASA PANGASIID (PANGASIANODON HYPOPHTHALMUS) FROM FAMILY SELECTION RESULTS Tahapari, Evi; Darmawan, Jadmiko; suharyanto, Suharyanto
Indonesian Journal of Tropical Aquatic Vol 3, No 1 (2020): February
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v3i1.11217

Abstract

Perkasa pangasiid (Pangasianodon hypophthalmus) is a superior catfish selected by the family that has been released by the Minister of Marine and Fisheries as aquaculture fish which has a selection response of 38.86 %. Genetic quality improvement to accelerate growth can be done through selection activities. The performance test of the Siamese catfish from the selection (Perkasa strain) compared to the non-selection strain (local strain) originating from the farmer. The purpose of this study was to compare the productivity of selection and non-selection Siamese catfish cultivation maintained in a net pond. The initial seeds of the test fish were 3.76 g to 3.79 g with a total length of 2 inches to 3 inches kept in a net pond measuring 3 × 5 × 1.25 m. The water level was around 100 cm with a density of 175 fish/net. Maintenance was carried out for six months. Test parameters observed included: weight gain, specific growth rate, biomass harvest, productivity and cost of production. The results showed that Siamese catfish which were selected for six months of maintenance had a final weight of 698.40 ± 164.54 g and non-selection strains of 595.77 ± 103.41 g. As well as having a higher specific growth rate (3.51 %), higher harvest biomass (11.44 %), and higher productivity (18.71 %) than non-selection Siamese catfish. Based on the bioeconomic analysis, the selection of enlarged Siamese catfish cultivation can reduce production costs up to Rp 390 /kg. The use of Perkasa Pangasiid superior seeds can increase harvest biomass higher than the non-selection seeds.
EFFECT OF ADDITION DIFFERENT LEVELS OF SOYBEAN OIL IN COMMERCIAL FISH FEED ON GROWTH PERFORMANCE, FEED EFFICIENCY, EPA AND DHA FOR PERKASA PANGASIID (Pangasianodon hypophthalmus Sauvage, 1878) Arifah, Fajar Nurul; Rostika, Rita; Herawati, Titin; Pratiwy, Fittrie Meyllianawaty; Darmawan, Jadmiko
AQUASAINS Vol 11, No 2 (2023)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v11i2.p1321 - 1332

Abstract

Pangasius is a freshwater fish included in the main commodity by the Ministry of Marine Affairs and Fisheries, Indonesia. Siamese catfish production ranked 4th in freshwater fish commodities in 2016 after goldfish, tilapia, and catfish. Pangasius has a lack of EPA (Eicosapentaenoic acid) and DHA (Docosahexaenoic acid) if compared with fish from the sea. Application of additional different levels of soybean oil in commercial fish fed to enhance EPA (Eicosapentaenoic acid) and DHA (Docosahexaenoic acid) in Perkasa pangasiid flesh. This study was conducted to determine the effect of adding soybean oil with different levels of commercial fish feed given to Perkasa striped catfish on growth performance, feed efficiency, EPA (Eicosapentaenoic acid), and DHA (Docosahexaenoic acid). This experimental study used a Complete Randomized Design (CDR) with triplicates and five treatments with additional levels of soybean oil in commercial fish fed: (a) 0%, (b) 2%, (c) 4%, (d) 6%, and (e) 8%. The result showed that treatment of (e) is the best treatment for increasing the EPA (Eicosapentaenoic acid) and DHA (Docosahexaenoic acid) in Pangasius flesh. However, additional different levels of soybean oil in the commercial fish feed have no negative effect on growth performance and feed efficiency in Perkasa pangasiid.
Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry Imron; Tahapari, Evi; Darmawan, Jadmiko; Muhammad Luthfi Abdurachman
Jurnal Akuakultur Indonesia Vol. 19 No. 2 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.19.2.142-152

Abstract

Nuclear DNA content (NDC) of species or population is believed to have been formed naturally by many mechanisms such chromosomal mutation, insertion and deletion, transposable element, and duplication. Additionally, hybridizations and species’ phylogenetic relationship may also contribute to the NDC diversity. This study was aimed to investigate the profile of NDC in four species Asian catfishes of the genera Pangasius including Pangasionodon hypophthalmus, Pangasius djambal, Pangasius nasusutus, Pangasius nieuwenhuisii, interspecific hybrid of female P. hypophthalmus and male P. djambal (Hybrid HD), and female P. hypophthalmus and male P. nasutus (Hybrid HN). Red blood cells (RBC) were taken from the respective species/groups and NDC measurement was performed in an Attune acoustic flowcytometer (ABI) using DAPI staining and chicken, Gallus domesticus, RBC was used as size reference. The results showed that the mean NDC of P. hypophthalmus, P. djambal. P. nasusutus, P. nieuwenhuisii, were 0.960±0.0254 pg, 1.017±0.0510 pg, 1.000±0.0410 pg, 1.074±0.0231 pg, which are within the range of NDC in the other catfish families The NDC values of Hybrid HD and Hybrid HN were1.005±0.0358 and 0.956± 0.0089, respectively. Among the pure line species, the NDC of P. hypophthalmus was the lowest and was different (P<0.05) from those of P. djambal and P. nieuwenhuisii but was not different (P>0.05) from that of the P. nasutus. The NDC of both Hybrid HD and Hybrid HN were not different form their respective parental lines. However, the NDC profiles of both hybrids were different in that the NDC of the former was in between while the latter was below their respective parental lines. Phylogenetically, the NDC diversity within Pangasiid catfish in this study was independent of their phylogenetic relationship based on cytoplasmic and nuclear markers. Keywords: Flow cytometry, nuclear DNA content, P.hypophthalmus, P. djambal, P. nasutus, P. nieuwenhuisii, interspecific hybrid.