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Antibacterial Activity and Potential Of Natural Textile Dyes From Sea Water Bacteria Syaifudin, Andang; Sutrisno, Sutrisno
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13446

Abstract

This research aims to determine the potential of bacterial isolates from Marina beach seawater to be used as natural textile dyes and to test their antibacterial ability. The media used for culturing bacteria is a zobel synthesis medium with a mixture of seawater. The results of the isolation and extraction of color pigments obtained a red color with a characteristic wavelength of 535 nm and has a variety of chemical content results. Pigment immersion trials using 3 types of fabric, namely cotton, primisima and dobby fabric. The results of immersion with ethanolic solvent obtained optimum results for 12 hours and with the addition of mordant (Fe.H2O4S.7H2O), the color of the fabric is strong enough to withstand washing with detergent and sunlight. From the results of the study, data showed that the dyed primisima fabric gave a reduction or inhibition of the growth of E. coli bacteria by 9% while the inhibition of S. aureus bacteria was 116%. The pigment has the potential to be used as a dye for batik cloth with the provisions of optimizing to find the right reinforcement or mordant. This study has implications for the discovery of red pigment-producing bacteria from the isolation of seawater at Marina Semarang beach.
Antibacterial Activity and Potential Of Natural Textile Dyes From Sea Water Bacteria Syaifudin, Andang; Sutrisno, Sutrisno
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 1 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i1.13446

Abstract

This research aims to determine the potential of bacterial isolates from Marina beach seawater to be used as natural textile dyes and to test their antibacterial ability. The media used for culturing bacteria is a zobel synthesis medium with a mixture of seawater. The results of the isolation and extraction of color pigments obtained a red color with a characteristic wavelength of 535 nm and has a variety of chemical content results. Pigment immersion trials using 3 types of fabric, namely cotton, primisima and dobby fabric. The results of immersion with ethanolic solvent obtained optimum results for 12 hours and with the addition of mordant (Fe.H2O4S.7H2O), the color of the fabric is strong enough to withstand washing with detergent and sunlight. From the results of the study, data showed that the dyed primisima fabric gave a reduction or inhibition of the growth of E. coli bacteria by 9% while the inhibition of S. aureus bacteria was 116%. The pigment has the potential to be used as a dye for batik cloth with the provisions of optimizing to find the right reinforcement or mordant. This study has implications for the discovery of red pigment-producing bacteria from the isolation of seawater at Marina Semarang beach.
Kelola Limbah Berbahaya Menjadi Barang Multiguna Melalui Pelatihan Pembuatan Sabun dari Minyak Jelantah di Kampung Malon Syaifudin, Andang; Aris Munandar, Nadaa Haniyyah; Muhtarom, Ahmad Amiruddin; Yansah, Ahmad Febri; Reza, Muhammad; Lutvia Rizmasari, Alifta Dina; Larasati, Winda; 'Aini, Munadiya Qurrotul
Jurnal Pengabdian Masyarakat Indonesia Vol 4 No 2 (2024): JPMI - April 2024
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.2049

Abstract

Permasalahan lingkungan menjadi salah satu fokus utama dalam pembangunan berkelanjutan. Peningkatan aktivitas manusia berdampak pada meningkatnya limbah berbahaya yang berpotensi mengancam kelestarian lingkungan. Minyak jelantah termasuk limbah Bahan Berbahaya dan Beracun (B3) yang dihasilkan oleh kegiatan rumah tangga. Masyarakat di Kampung Malon belum mengelola dengan baik limbah minyak jelantah, oleh karena itu perlu diadakan kegiatan pelatihan pengelolaan minyak jelantah. Pengelolaan ini menjadi penting karena meningkatnya jumlah penduduk dan intensifikasi aktivitas manusia sehingga potensi kerusakan yang diakibatkan akan semakin besar jika tidak ditangani. Metode pengabdian yaitu berupa penyuluhan dan praktik pembuatan yang diolah menggunakan metode deskriptif-eksplanatif. Salah satu produk olahan minyak jelantah yaitu sabun. Kegiatan pelatihan ini melalui tahap persiapan dan pelaksanaan. Kegiatan pengabdian ini dapat meningkatkan pemahaman dan kesadaran masyarakat pentingnya mengelola limbah B3 menjadi barang yang lebih bermanfaat dan memiliki nilai ekonomis. Melalui kegiatan pelatihan ini, masyarakat memahami bahaya membuang minyak jelantah begitu saja ke lingkungan dan mampu membuat sabun dari minyak jelantah.
Pemberdayaan Masyarakat Dalam Upaya Mandiri Pangan Melalui Hidroponik Syaifudin, Andang; Astuti, Fitri Puji; Dyana, Hanif; Azam, Abdullah; Fauziah, Alvi; Roshif, Mohammad Qoys; Haikal, Ahmad; Latifah, Afni Nur
Prima Abdika: Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2024): Volume 4 Nomor 1 Tahun 2024
Publisher : Program Studi Pendidikan Guru Sekolah Dasar Universitas Flores Ende

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/abdika.v4i1.3324

Abstract

People's need for food, such as fruit and vegetables, is increasing every year along with population growth. Efforts currently being made to meet the self-sufficient needs of the community's food are by importing, because local products have not been able to meet the food needs of the community due to the not yet optimal level of land productivity in Indonesia so that the hydroponic system is the most appropriate to address food problems because hydroponics is a cultivation system with take advantage of narrow land. The hydroponic training aims to support the introduction of more efficient and sustainable farming methods in the context of limited land and resources and then provide an understanding of farming using the hydroponic method for the people of Candi Baru RT 06/04, Genting village, Boyolali district. The MMK KKN Hydroponic training activities (special mission independent) were held on July 31, 2023. It was held in Dukuh Candi Baru Village, RT 06/04, Kec. Genting district. Boyolali coincided with a local resident's house. The hydroponic technique taught is a simple technique, namely thewick system, plant seeds taught in the hydroponic technique, namely kale, spinach, and bok choy. The results of the questionnaire recap show that the knowledge level of the participants has increased, this is shown by the graph which shows the level of knowledge after the training has increased above 80%. This shows the success of the training carried out by the Mandiri KKN Special Mission UIN Walisongo Semarang for the participants.
Pemberdayaan Masyarakat Desa Dalam Pemanfaatan Sampah Organik Melalui Pelatihan Pembuatan Pupuk Organik Syaifudin, Andang; Avitasari, Ratna; Lestari, Yunita Dwi; Lutfi, Jihan; Maula, Indah Safaatul; Mauludi, Ahmad; Karnila, Karnila; Nadhiroh, Ayu Lailatun; Sari, Septina Putri Nur Wulan; Astuti, Fitri Puji
Prima Abdika: Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2024): Volume 4 Nomor 1 Tahun 2024
Publisher : Program Studi Pendidikan Guru Sekolah Dasar Universitas Flores Ende

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/abdika.v4i1.3326

Abstract

Soil conditions vary from region to region. Some are fertile, while others are less fertile. Factors such as nutrient imbalance, soil depletion, decreased organic content, and microbial activity affect soil fertility. To maintain the balance of soil nutrients and organic matter, it is necessary to add organic fertilizers to harmonize the nutrient content of the soil. Chemical fertilizers only provide one type of plant nutrient without improving soil properties. Socialization of organic fertilizers is needed so that people are wiser in maintaining soil fertility with organic materials. The community service was carried out to provide training and practice in making organic fertilizer from livestock manure and plant residues in Duku Candi Baru, Genting Village, Cepogo District, Boyolali Regency. This service activity uses two approaches, namely counseling and training. The extension stage is carried out by providing material about processing cow dung into organic fertilizer. Training is carried out by demonstrating how to make organic fertilizer in a simple way. This training begins with the socialization of making organic fertilizer and compost, which aims to make the community understand first about information about organic fertilizer and compost along with its benefits, then continued with the practice of making organic fertilizer and compost. This training educates the community about the use of organic waste for organic fertilizer in sustainable agriculture. Through a mixture of simple materials such as cow dung, vegetable waste, EM4, and molasses, it is hoped that the community will use organic fertilizers rather than chemical fertilizers for more environmentally friendly agricultural products.
Aktivitas Antibakteri Isolat Jamur Endofit Kitolod (Hippobroma longiflora (L.) G. Don.) terhadap Bakteri Escherichia coli dan Staphylococcus aureus Pramesta, Carerina Oktavia; Syaifudin, Andang; Kusumarini, Niken
Journal of Natural Sciences Vol 6, No 3 (2025): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i3.1031

Abstract

Endophytic fungi are microorganisms that inhabit plant tissues without causing harm to their hosts. Hippobroma longiflora (L.) G. Don (kitolod) is known to contain secondary metabolites with antibacterial potential. This study aimed to isolate and evaluate the antibacterial activity of endophytic fungi from the leaves and flowers of kitolod against Escherichia coli and Staphylococcus aureus. Isolation was performed by surface sterilization using 1% NaOCl, 70% ethanol, and sterile water, followed by cultivation on PDA medium. Nine isolates were obtained, consisting of four from flowers and five from leaves. Extracts from PDB cultures were tested using the disc diffusion method (30 µL/disc). Results showed that isolate BK1 exhibited strong antibacterial activity against S. aureus (11.16 mm) and moderate activity against E. coli (9.71 mm). Isolates BK3 and DK3 also displayed strong inhibition zones. The positive control (chloramphenicol) produced very strong inhibition, while the negative control (sterile water) showed none. The antibacterial activity is presumed to originate from secondary metabolites of endophytic fungi, indicating their potential as natural antibacterial sources. Further studies are needed to determine MIC values and conduct molecular identification of the selected isolates.
Endophytic fungi from Parijoto (Medinilla speciosa) leaves and their potential as biocontrol agents against Corn Pest Larvae Syaifudin, Andang; Wahidah, Baiq Farhatul
Journal of Natural Sciences and Mathematics Research Vol. 11 No. 2 (2025): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.v11i2.28768

Abstract

Parijoto (Medinilla speciosa) leaves are widely used for their antimicrobial properties. Endophytic fungi from these leaves show potential as entomopathogenic agents against Helicoverpa armigera larvae, a moth pest of cotton and corn.The research activities conducted in the laboratory include the isolation and characterization of endophytic fungi, as well as entomopathogenic testing against H. armigera larvae using a spraying technique with endophytic fungal extracts. In this study, 5 isolates were obtained from leaves and assessed their entomopathogenic properties. The isolate code is EP1, EP2, EP3, EP4, EP5. Bioassays were conducted to evaluate the effectiveness of these fungi in controlling H. armigera larvae. Among the tested fungi, EP5 demonstrated the highest entomopathogenic activity, significantly reducing the survival rate of the larvae. These findings indicate that EP5 holds substantial promise as a biocontrol agent for managing H. armigera populations with mortality percentage 30,78%, offering a sustainable and environmentally friendly alternative to chemical pesticides. The study underscores the potential of utilizing endophytic fungi from parijoto leaves in integrated pest management strategies.
Identifikasi keanekaragaman fungi tanah di hutan Mangrove Pantai Alam Indah Kota Tegal Ulwiyah, Ulwiyah; Syaifudin, Andang
Jurnal Esabi (Jurnal Edukasi dan Sains Biologi) Vol. 7 No. 2 (2025)
Publisher : Universitas Bung Hatta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37301/esabi.v7i2.85

Abstract

Ekosistem hutan Mangrove menyediakan tempat unik bagi kehidupan mikroorgnisme, termasuk fungi didalamnya.  Salah satu pantai yang memiliki hutan Mangrove adalah Pantai Alam Indah Kota Tegal. Namun kajian tentang keragaman fungi di hutan Mangrove Pantai Alam Indah Kota Tegal belum dilakukan. Penelitian ini bertujuan untuk mengetahui jenis-jenis fungi yang terdapat di hutan Mangrove Pantai Alam Indah Kota Tegal dan keanekaragaman fungi tanah di Hutan Mangrove Pantai Alam Indah kota Tegal. Penelitian ini merupakan penelitian eksperimental, isolat fungi didapat dari sampel tanah yang diisolasi dari 6 lokasi dengan teknik purposive sampling. Sampel diisolasi dan diinkubasi, kemudian diamati dan diidentifikasi. Adapun jenis fungi yang teridentifikasi adalah Aspergillus sp 1, Aspergillus sp 2, Aspergillus sp 3, Aspergillus sp  4, Aspergillus sp 5, Aspergillus sp 6, Penicillium sp, Mucor sp, Fusarium sp, Sepedonium sp, Verticillium sp dan Curvularia sp. Keanekaragaman fungi tanah di Hutan Mangrove Pantai Alam Indah termasuk dalam kategori sedang dengan nilai indeks keanekaragaman 1,893. Kemerataan jenis Fungi di hutan Mangrove Pantai Alam Indah termasuk tinggi dengan nilai total indeks kemerataan 0,762. Penicillium sp (33%) dan Aspergillus sp 2 (28%) adalah jenis fungi yang mendominasi.