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All Journal Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Riset Industri Hasil Hutan ULIN: Jurnal Hutan Tropis Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Suwinarti, Wiwin
Fakultas Kehutanan, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Aktivitas antioksidan dan antibakteri dari ekstrak bee pollen lebah kelulut (Tetragonula sarawaknensis) Sari, Ayu Mitha; Rosamah, Enih; Suwinarti, Wiwin; Kusuma, Irawan W; Arung, Ph.D., Enos Tangke
Jurnal Riset Industri Hasil Hutan Vol 13, No 2 (2021)
Publisher : Kementerian Perindustrian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24111/jrihh.v13i2.7050

Abstract

Pemanfaatan produk propolis dan bee pollen madu tanpa sengat atau Kelulut masih sangat terbatas, demikian juga penelitiannya belum banyak dilakukan bahkan bisa dikatakan masih sangat terbatas termasuk kandungan zat ekstraktif pada bee pollen tersebut. Oleh karena itu tujuan penelitian ini adalah untuk skirining manfaat bee pollen sebagai produk kecantikan dan produk yang mengandung bahan yang dapat menangkal radikal bebas. Kemajuan teknologi dan gaya hidup manusia dimasa sekarang cenderung mudah menimbulkan banyak penyakit baru. Hal ini menimbulkan rasa penasaran akan bee pollen sebagai produk dari alam yang memiliki kemampuan sebagai antioksidan dan menghambat pertumbuhan Propionibacterium acnes. Metode ekstraksi yang digunakan dalam penelitian ini adalah ekstraksi dingin etanol 98% selama 3 x 24 jam, dilanjutkan dengan uji fitokimia, dan analisis antioksidan dengan uji DPPH dan penghambatan Propionibacterium acnes dengan metode difusi agar. Ekstrak bee pollen T. sarawakensis mengandung senyawa alkaloid, flavonoid, kumarin dan tanin. Ekstrak T. sarawakensis memiliki aktivitas antioksidan dengan nilai konsentrasi hambatan 39% pada 100 mg/mL. Ekstrak memiliki penghambatan terkuat dari Propionibacterium acnes (42% pada 500 µg/well). Hasil ini menunjukkan adanya potensi ekstrak etanol T. sarawakensis untuk dikembangkan sebagai bahan kosmetik dan jamu, namun diperlukan percobaan lebih lanjut untuk membuktikan fungsinya.
A Study on the Production Method of Kenaf High Fiber Strength Wiwin Suwinarti; Zhou Chen; Susumu Nakahara; Ryo Sugawara; Kazuhiko Sameshima
Wood Research Journal Vol 2, No 2 (2011): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2011.2.2.83-88

Abstract

Kenaf bast fiber from 58 varieties grown in Zhejiang province, China, was treated using three treatments. First treatment used ammonium oxalate, sodium hydroxide and acidic chlorite (AT3), second treatment used ammonium oxalate and sodium hydroxide (AT0) and the third one was retting treatment (RET). Some parameters such as diameter, height and weight of stalk, and also fiber strength were measured for screening method.Treatment AT3 show much better strength than the other two. It means the single fiber which belongs to AT3 is stronger than the bundle fiber of AT0 and RET. Moreover the chemical treatment of AT0 has much stronger bundle fiber than RET. The best-8 varieties (kenaf sample number 8, 11, 12, 30, 32, 48, 52 and 58) were selected based on the fiber strength and kenaf sample number 12 and 32 reached the superior two if evaluated with other factors.
A Study on the Production Method of Kenaf High Fiber Strength Wiwin Suwinarti; Zhou Chen; Susumu Nakahara; Ryo Sugawara; Kazuhiko Sameshima
Wood Research Journal Vol 2, No 2 (2011): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2011.2.2.83-88

Abstract

Kenaf bast fiber from 58 varieties grown in Zhejiang province, China, was treated using three treatments. First treatment used ammonium oxalate, sodium hydroxide and acidic chlorite (AT3), second treatment used ammonium oxalate and sodium hydroxide (AT0) and the third one was retting treatment (RET). Some parameters such as diameter, height and weight of stalk, and also fiber strength were measured for screening method.Treatment AT3 show much better strength than the other two. It means the single fiber which belongs to AT3 is stronger than the bundle fiber of AT0 and RET. Moreover the chemical treatment of AT0 has much stronger bundle fiber than RET. The best-8 varieties (kenaf sample number 8, 11, 12, 30, 32, 48, 52 and 58) were selected based on the fiber strength and kenaf sample number 12 and 32 reached the superior two if evaluated with other factors.
UJI IN VITRO GABUNGAN EKSTRAK TANAMAN DARI KALIMANTAN, INDONESIA TERHADAP BAKTERI PATOGEN PADA IKAN NILA Esti Handayani Hardi; Gina Saptiani; Nurkadina Nurkadina; Irawan Wijaya Kusuma; Wiwin Suwinarti
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.452 KB) | DOI: 10.19087/jveteriner.2018.19.1.35

Abstract

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.
Efektivitas perlakuan awal alkali dan hidrotermal terhadap proses delignifikasi, potensi gula reduksi dan produksi etanol teoritis dari kayu Vernonia amygdalina Delile Mardiatul Ufa; Nasriati N; Aulia Fitria Ningrum; Wiwin Suwinarti; Rudianto Amirta
ULIN: Jurnal Hutan Tropis Vol 6, No 2 (2022)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v6i2.7438

Abstract

Vernonia amygdalina merupakan salah satu jenis tanaman Short Rotation Coppice (SRC) atau trubusan yang secara alami dapat melakukan proses regenerasi setelah melalui proses pemangkasan. Selain itu juga sebagai bahan baku penyedia biomassa yang saat ini banyak digunakan dalam memproduksi energi di dunia. Oleh karena itu, V. amygdalina memiliki potensi pembangkitan energi yang besar dan memadai untuk digunakan salah satunya adalah sebagai bahan baku untuk produksi bioetanol. Penelitian ini bertujuan untuk mengetahui efektivitas perlakuan awal yang dilakukan dengan menggunakan konsentrasi alkali dan waktu reaksi yang berbeda terhadap proses sakarifikasi dan gula tereduksi yang dihasilkan beserta potensi teoritis dari etanol yang diperoleh. Biomassa kayu terlebih dahulu diolah dengan autoklaf pada berbagai konsentrasi NaOH (0, 1, 2, dan 3% (g/L)) dan waktu reaksi (15, 30, 45, dan 60 menit). Sampel kayu yang telah diolah kemudian dihidrolisis menggunakan enzim selulase komersial untuk menghasilkan monomer glukosa. Waktu hidrolisis pada 12, 24, 36, dan 48 jam dianalisis. Hasil penelitian menunjukkan bahwa penambahan NaOH 3% pada waktu reaksi 15 menit dan waktu hidrolisis 36 jam merupakan konsentrasi terbaik untuk memberikan potensi etanol tertinggi (186,98 L/ton biomassa kayu). Akhirnya, penelitian ini menunjukkan bahwa V. amygdalina akan menjadi bahan baku yang menjanjikan untuk produksi etanol.
AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA SARANG BURUNG WALET (Collocalia Fuciphaga) DARI BERBAGAI JENIS KELAS SARANG DAN JENIS BANGUNAN Assegraff , Andi Syahid Khalid; Suwinarti, Wiwin; Wardhani, Isna Yuniar; Budi, Agus Sulistyo; Kusuma, Irawan Wijaya; Arung, Enos Tangke
Jurnal Ilmiah Manuntung Vol 10 No 2 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i2.804

Abstract

The community has traditionally utilized the swallow’s nest (Collocalia fuciphaga), an edible bird’s net (EBN), as a component in medicines and cosmetics. Within the cave’s walls lies the environment for the swallow’s nest. The nest’s quality and form are taken into account when determining the nest wallet’s selling price. This study intends to ascertain the possible suppression of the bacteria Staphylococcus sp. and Escherichia sp. activity as well as the antioxidant activity of the swallow’s nest from the nest class formed from wood and concrete construction types. The study was conducted directly on dry, hair- and dirt-free, finely powdered, and dissolved in distilled water swallow nests (Collocalia fuciphaga). Testing for antibacterial activity was conducted. Agar well testing for antibacterial activity was carried out using Mueller Hinton Agar (MHA) and chloramphenicol media as positive controls. Ascorbic acid was used as a positive control while 1,1-diphenyl-2-picrylhydrazyl (DPPH) was tested for its ability to scavenge free radicals. The test findings demonstrated that the swallow (Collocalia fuciphaga) had substantial antioxidant activity against DPPH and that the swallow’s nest (Collocalia fuciphaga) had antibacterial activity against Staphylococcus sp. and Escherichia sp. germs. The swallow’s nest’s (Collocalia fuciphaga) antioxidant and antibacterial properties are specific to different nest classes and building kinds, so they may be utilized as a foundation for thought about raising the selling price of bowls, powders, and crumbles in each form of structure.
Uji aktivitas antibakteri ekstrak propolis lebah Heterotrigona itama dari beberapa lokasi budidaya di Kalimantan Timur terhadap bakteri Propionibacterium acnes Arung, Enos Tangke; Dikarulin, Sarah Azzara; Dewi Listyaningrum, Dyah Ayu; Ananda, Bayu Susetya; Putri, Tasya Ananda; Amirta, Rudianto; Yuliansyah, Yuliansyah; Kusuma, Irawan Wijaya; Ramadhan, Rico; Rosamah, Enih; Suwinarti, Wiwin
ULIN: Jurnal Hutan Tropis Vol 6, No 2 (2022)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v6i2.7824

Abstract

Propolis merupakan produk penting bagi lebah yang banyak digunakan sebagai salah satu alternatif pengobatan alami karena mengandung senyawa bioaktif yang dapat memberikan efek positif pada tubuh. Sebagai produk yang dihasilkan lebah untuk menutupi sarangnya dan mencegah predator maupun infeksi bakteri atau virus dari luar, propolis memiliki salah satu manfaat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak propolis lebah Heterotrigona itama yang berasal dari 4 lokasi budidaya (perkebunan, persawahan, hutan sekunder, dan perkotaan) di Kalimantan Timur terhadap bakteri Propionibacterium acnes atau P.acnes dengan metode difusi lubang/sumuran pada media nutrient broth (NB) dengan kloramfenikol sebagai kontrol positif dan aseton sebagai kontrol negatif. Propolis diekstrak menggunakan metode maserasi dengan larutan etanol 96% yang kemudian dibuat dalam 3 (tiga) konsentrasi yaitu 500ppm, 250 ppm, dan 125 ppm. Hasil penelitian menunjukanfitokimia dari propolis di 4 lokasi budidaya dominan mengandung alkaloid, flavonoid dan kumarin. Uji bakteri pada konsentrasi 500 ppm,propolis H.itama dari lokasi perkebunan dan persawahan tergolong dalam kategori kuat dalam menghambat pertumbuhan bakteri, dari lokasi hutan sekunder tergolong sedang, dan dari lokasi perkotaan tergolong rendah.
Prevention Methods Against Aeromonas Hydrophila and Pseudomonas fluorescens Infection in Tilapia Hardi, Esti Handayani; Nugroho, Rudi Agung; Kusuma, Irawan Wijaya; Suwinarti, Wiwin; Anjani, Ryan; Aziza, Aziza
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1425.045 KB) | DOI: 10.11598/btb.0.0.0.1131

Abstract

This research evaluated a method involving provision of a concoction of Boesenbergia pandurata, Solanum ferox dan Zingimber zerumbet extracts for pathogen prevention in tilapia. The concentration of each extract was 600 ppm of Boesenbergia pandurata/BP, 900 ppm of Solanum ferox/SF and 200 ppm of Zingimber zerumbet/ZZ. The examination was performed by issuing two combinations of extracts (SF:BP, SF:ZZ) against Aeromonas hydrophila and Pseudomonas fluorescens (105 CFUmL-1). Preventive trials were carried out by providing a concoction of extracts through intraperitoneal injection (0.1 mL/fish) in tilapia (15±2 g) and the immersion method was performed by bathing the fish in the extracts for 20 minutes, with pathogen challenging during the following 24 h being carried out. The composition of the used extract was by SF60:ZZ40; SF50:ZZ50; BP90:SF10; BP50:SF50; and fish without being given the extract. Haematology and immunology parameters were observed at the 4th week after challanges with pathogenic bacteria. The number of white blood cells (WBCs) increased significantly (P <0.05) compared to controls without extract, with a similar increase observed for red blood cell (RBCs), but heamatocrit (Ht) and hemoglobin (Hb) values did not significantly increase compared to control. Phagocytic index, respiratory burst and lysozyme activities also experienced a significant increase in fish fed with combined extracts compared to controls. The numbers of pathogenic bacteria in the body of the fish given extract were also lower than the control and significantly different at the 4th week. The results of this study indicate that giving combined extracts of SF50:ZZ50 and BP90:SF10 provides the best protection (RPS) against infection of A. hydrophila and P. fluorescent by injection of 100%. This study indicates that providing combined extracts by injection and immersion in the ratio of SF50:ZZ50 has a positive effect in increasing the non-specific immune system of tilapia and increasing protection against bacterial infections.
Co-Authors Agus Sulistyo Budi Amirta, Rudianto Ananda, Bayu Susetya Anjani, Ryan Assegraff , Andi Syahid Khalid Aulia Fitria Ningrum Aziza, Aziza Dewi Listyaningrum, Dyah Ayu Dikarulin, Sarah Azzara Enos Tangke Arung, Enos Tangke Esti Handayani Hardi Gina Saptiani Irawan Wijaya Kusuma, Irawan Wijaya Isna Yuniar Wardhani Kazuhiko Sameshima Kusuma, Irawan W Mardiatul Ufa Nasriati N Nugroho, Rudi Agung Nurkadina Nurkadina Putri, Tasya Ananda Rico Ramadhan, Rico Rosamah, Enih Rosamah, Enih Rudianto Amirta Ryo Sugawara Sari, Ayu Mitha Susumu Nakahara Yuliansyah Yuliansyah Zhou Chen