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All Journal Jurnal Akuakultur Rawa Indonesia Jurnal Veteriner Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Perikanan Universitas Gadjah Mada Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan Jurnal Perikanan

Gina Saptiani

Universitas Mulawarman

Author-ID : 5482580

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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UJI IN VITRO GABUNGAN EKSTRAK TANAMAN DARI KALIMANTAN, INDONESIA TERHADAP BAKTERI PATOGEN PADA IKAN NILA Esti Handayani Hardi; Gina Saptiani; Nurkadina Nurkadina; Irawan Wijaya Kusuma; Wiwin Suwinarti
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.

Patogenesitas Bakteri Enterobacteriaceae pada Ikan Zebra (Danio rerio) Sebagai Hewan Model Widyaningsih Rahayu; Esti Handayani Hardi; Gina Saptiani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Enterobacteriaceae are Gram negative bacteria contain endotoxin and exotoxins which arerequirements for pathogenic bacteria and act as opportunistic pathogens. The purpose of this research was to determine the ability of Enterobacteriaceae bacteria to infect zebrafish (Danio rerio) by observing anatomical pathology, mortality, time death and cumulative time of death. The method used in thisresearch is a completely randomized design method (CRD). This research was conducted in three stages, first preparation of zebrasfish as animal model by average size 3-5cm and reach three months old. Second, bacterial cultures from 10 species of Enterobacteriaceae were Escherichia coli 1, E. coli 2, Enterobactercloacae 1, Acinetobacter calcoaceticus, Enterobacter amnigenus 1, Enterobacter amnigenus 1, Escherichia coli 3, Pantoea spp., E. cloacae 1, E. cloacae 2, E. cloacae 3, cultured in Brain Heart Infused Broth (BHIB) and later media washed with Phospate Buffer Saline (PBS) 0.45%. The third stage was bacterial infection to zebrafish using immersion method with each treatment repeated 3 times and observed for 120 hours. The results showed 10 species that used caused death (mortality), the highest mortality in fish that infected with E. coli 2 and E. cloacae 1 with a percentage of 66.67% infected by E. coli 2 and E. cloacae 1. Anatomicalpathology in the external organs and internal organs showed post-infectious symptoms. Infection occurred with mean time to death of 12-86 hours, as long as the cumulative time of fish death caused by E. coli 2, A.cinetobacter calcoaceticus and E. cloacae 1 causing death from 24 hours after infection until the end ofobservation.

POTENSI Avicenia marina UNTUK MENGHAMBAT MICROBA DAN MELINDUNGI BENUR UDANG WINDU Gina Saptiani; Andi Noor Asikin; Fikri Ardhani; Esti Handayani Hardi
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The research aims to study potency of Avicennia marina leaf extract to inhibit pathogens in vitro and in vivo on post larvae of tiger prawn. The leaves we re chopped, dried and extracted with water solvents, sea water andethanol. In vitro inhibitory tests were performed using agar disc diffusion (ADD) and minimal inhibitory concentration (MIC) methods. The in vivo test on tiger prawn PL-8 was given by submersion, which then tested with Vibrio harveyi and Saprolegnia sp. The in vitro test showed A. marina extract can inhibit V.harveyi and Saprolegnia sp. In vivo tests of V. harveyi and Saprolegnia sp. infections, showed 60- 88% survival and 57.33-86.67%, with protective ability of the highest pathogen infection of 80.80% and 77.80%. The best A. marina extract that can inhibit microbial and protect the tiger prawn from pathogen infection is ethanol extract with concentration 1,250-1,500 ppm, followed by sea water extract 1,500 ppm and water extract 1,500 ppm respectively

Susceptibility of Tiger Shrimp (Panaeus monodon) against Vibrio harveyi on various Molt Stage and Osmolarity Gina Saptiani; Catur A Pebrianto; Agustina Agustina; Esti H Hardi
Jurnal Perikanan Universitas Gadjah Mada Vol 18, No 1 (2016)
Publisher : Universitas Gadjah Mada

Vibrio harveyi causes disease of tiger shrimp (Penaeus monodon) aquaculture in East Kalimantan. This research aimed to investigate the susceptibility of tiger shrimp on various molt stage and osmolarity, and determine the salinity (osmolarity) of water that can reduce the risk of V. harveyi attack. Shrimp age of 1.5 months was maintained in 4 aquariums with salinity 29.72 ‰, 26.07 ‰, 22.35 ‰ and 17.79 ‰, then challenged with V. harveyi. After 7 and 14 days, osmolarity of haemolimph was observed with automatic osmometer and Digital Roebling Osmometer. The susceptibility of tiger shrimp to V. harveyi was evaluated based on clinical symptoms, pathological anatomy of shrimp and density of V. harveyi (TPC) in the hepatopancreas. Osmolarities of shrimp haemolymph at premolt, postmolt, and intermolt stage were 575.30-812.60; 534.00-788.80, and 566.20-795.60 mOsm/l H2O, respectively. The lowest bacterial content in the premolt stage was maintained at a salinity 22.35 ‰, and the highest one was obtained in postmolt stage at 29.72 ‰. The molting and postmolt stage were the most susceptible to V. harveyi attacks. The results suggested that the shrimp maintained at 22.35 ‰ salinity was less susceptible to V. harveyi.

Suplementasi vitamin c dalam pakan sebagai upaya peningkatan kesehatan ikan mas (Cyprinus carpio L.) dalam menghadapi infeksi bakteri Aeromonas hydrophila: Vitamin-C suplement in fish feeding substance in order to improve carp fish health against aeromonas hydrophila bacterial infection Agustina; Gina Saptiani; Rahmi Nohon
Jurnal Ilmu Perikanan Tropis Nusantara (Nusantara Tropical Fisheries Science Journal) Vol. 1 No. 2 (2022): Ilmu Perikanan Tropis Nusantara
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jipt.v1i2.433

This study was conducted to determine the effects of different levels of vitamin C (ascorbic acid) supplementation on NVC (Nutrition Value Coeficient), clinical signs, pathological change, phagocytic index, antibody, and survival rate of fingerlings common carp (Cyprinus carpio L.). Three diets were formulated incorporating vitamin C at levels of 0 (control), 250 and 500 mg/ kg. Fish were randomly fed with the experimental diets for 35 days. On 15th day fish were injected intra muscular with 0.1 ml/fish, 106 CFU/ml concentration of A. hydrophila. The present study suggest that vitamin C concentration 250 mg/kg of diet most appropriate concentration, can enhance fish health (NVC), immune system (phagocytic index and antibody), survival rate, normal clinical signs and pathological change after infected by A. hydrophila.

Pertumbuhan dan percepatan molting kepiting bakau (Scylla serrata) yang diberi ekstrak temu kunci (Boesenbergia pandurata) 3 In 1 BIOIMUN® di tambak silvofishery Desa Salo Palai Kecamatan Muara Badak Kabupaten Kutai Kartanegara: The growth and accelerate molt of mud crab (Scylla serrata) given Boesenbergia pandurata extract in silvofishery ponds Salo Palai Village Muara Badak District Kutai Karanegara Regency Andre Jolpano; Esti Handayani; Gina Saptiani
Jurnal Ilmu Perikanan Tropis Nusantara (Nusantara Tropical Fisheries Science Journal) Vol. 2 No. 1 (2023): Ilmu Perikanan Tropis Nusantara
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jipt.v2i1.76

This research aimed to study the administrasion of B. pandurata extract mixed with crab feed on the percentage of molting, growth, and survival, and feed conversion ratio of mud crabs reared in silvofishery ponds. This research was conducted in Salo Palai Village, Muara Badak District, Kutai Kartanegara Regency. This study used a completely randomized design, with 4 treatments and 3 replications. The extract of B. pandurata in the form 3 In BIOIMUN® drug there are 4 treatment dose dasnamely (0 mL/kg, 20 mL/kg, 25 mL/kg, 30 mL/kg feed). The crabs used came from the catch of the Muara Badak community with a size of 200-280 g, and were culture for 4 weeks (30 days). The crabs were fed fresh fish with the addition of extract 2 times a day, in the morning (07.00-08.00) and afternoon (16.00-17.00), but feeding was done every 2 days. The feed given was weighed as much as 5% of the weight of the crab. The results showed that the administration of 3 In 1 BIOIMUN® in treatment P1 (20 mL/kg) affect the percentage of molting, growth, survival, and feed conversion ratio.

Isolat bakteri asam laktat untuk mengendalikan Aeromonas hydrophila pada ikan nila (Oreochromis niloticus): Isolates of lactic acid bacteria to control Aeromonas hydrophila in tilapia (Oreochromis niloticus) Sundari Hidayat; Gina Saptiani; Agustina
Jurnal Ilmu Perikanan Tropis Nusantara (Nusantara Tropical Fisheries Science Journal) Vol. 2 No. 1 (2023): Ilmu Perikanan Tropis Nusantara
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jipt.v2i1.250

Tilapia is one type of freshwater fish that has high economic value. With the increasing demand by the community, tilapia is cultivated in a semi-intensive system so that bacterial diseases caused by Aeromonas hydrophila arise. One way to treat disease in fish safely is the use of lactic acid bacteria. The purpose of this study was to examine the isolates of lactic acid bacteria in controlling diseases caused by Aeromonas hydrophila in tilapia. This study used a completely randomized design experimental method with 4 treatments and 3 replications. The treatments consisted of feed mixed with Phosphate Buffered Saline (PBS), Enterococcus faecalis isolate, Lactobacillus plantarum isolate and Lactococcus lactis isolate at a dose of 0.1 mL/g of feed at a concentration of 106 CFU/mL and mixed with 0.1 mL of egg white. The results showed that the treatment with lactic acid bacteria affected the condition of clinical symptoms and anatomical pathology of tilapia, but was better than the control treatment. The feed mixed with lactic acid bacteria also had an effect on increasing the immune response and showed higher survival than the control treatment. The condition of water quality in the rearing media showed the normal range for the life of tilapia.

POTENSI ANTIBAKTERI EKSTRAK DAUN JERUJU (Acanthus ilicifolius) TERHADAP Vibrio harveyi SECARA IN VITRO Gina Saptiani; Slamet Budi Prayitno; Sutrisno Anggoro
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Untuk mengetahui potensi antibakteri dan konsentrasi optimal jeruju (Acanthus ilicifolius), dilakukan uji daya hambat ekstrak dan fraksi daun terhadap pertumbuhan Vibrio harveyi, bakteri penyebab penyakit pada udang. Daun yang sudah dikeringanginkan diekstraksi dengan metanol, sehingga didapatkan ekstrak kasar (crude). Sebagian dari ekstrak kasar dilakukan fraksinasi dengan metode kolom silika gel dengan pelarut n-heksan, etil asetat, n-butanol, etanol, dan metanol. Uji daya hambat dilakukan dengan agar disc diffusion method. Perlakuan yang diberikan adalah ekstrak kasar, fraksi n-heksan, etil asetat, n-butanol, etanol, dan metanol yang masing-masing konsentrasinya 50-1000 ppm, yang diberikan pada kultur V. harveyi pada media Triptic Soy Agar (TSA). Fraksi etil asetat menunjukkan daya hambat terbaik (12 mm), diikuti ekstrak (11,33 mm), dan fraksi n-butanol (11 mm).

ANALISIS STRATEGI PROGRAM PASCATAMBANG PETERNAKAN SAPI PADA LAHAN BEKAS TAMBANG PT KITADIN SITE EMBALUT Eko Budi Prasetyo; Marlon Ivanhoe Aipassa; Taufan Purwokusumaning Daru; Gina Saptiani; Salaho Dina Devy; Kiswanto Kiswanto; Yosep Ruslim; Feby Kristina
Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Vol 22, No 2 (2023): Oktober 2023
Publisher : Universitas 17 Agustus 1945 Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31293/agrifor.v22i2.6816

Penelitian ini merupakan penelitian deskriptif dengan pendekatan kuantitatif. Penelitian deskriptif dengan pendekatan kualitatif untuk mengidentifikasi jenis-jenis program pascatambang peternakan sapi yang telah dilakukan oleh PT Kitadin serta untuk mengetahui kemajuan kegiatan program pascatambang peternakan sapi yang telah dilakukan oleh PT Kitadin. Penelitian ini bertujuan untuk menggambarkan bagaimana kegiatan yang telah dilakukan dalam rangka melaksanakan program pascatambang peternakan sapi dan bagaimana strategi pengembangan program peternakan sapi yang dilakukan oleh PT Kitadin. Adapun metode yang digunakan dalam pengumpulan data yakni observasi/kunjungan lapangan langsung, wawancara dan study pustaka. Dalam pengolahan data yang telah diperoleh dilakukan dengan cara membandingkan pelaksanaan program tersebut dengan dokumen program rencana pascatambang dan melakukan evaluasi terkait pengembangan peternakan sapi yang telah di lakukan. Berdasarkan hasil penelitian diketahui bahwa pelaksanaan program pascatambang peternakan sapi di lahan bekas tambang khususnya di Pit S12 GS telah sesuai dengan dokumen perubahan program rencana pascatambang PT Kitadin tahun 2020 dari segi program dan jadwal pelaksanaan, rencana luasan area, serta rencana peruntukannya. Adapun terkait Startegi pengembangan peternakan sapi di lahan bekas tambang khususnya Pit S12 GS yang dilakukan oleh PT Kitadin apabila dilihat dari aspek pemilihan lokasi, topografi, daya dukung tanah, ketersediaan pakan ternak, dan sumber air minum ternak telah memadai dan sesuai dengan peruntukannya, tetapi bila didasarkan pada daya tampung, maka luas lahan yang disediakan untuk program pascatambang peternakan sapi belum memadai yakni hanya mampu menampung sebanyak 620 ekor sapi Bali dari rencana maksimal sebanyak 1.600 ekor sapi Bali.

PATOGENITAS ISOLAT BAKTERI ASAM LAKTAT DARI USUS IKAN REPANG (Puntioplites waandersi) TERHADAP IKAN NILA (Oreochromis niloticus) Puput Andriani; Agustina Agustina; Gina Saptiani
Jurnal Akuakultur Rawa Indonesia VOL 11, NO 1 (2023): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v11i1.21109

This study aims to determine the pathogenicity of lactic acid bacteria from repang fish on clinical symptoms, anatomical pathology, hematological and survival rate of tilapia (Oreochromis niloticus). This reseach used a completely randomized design with 5 treatments and 3 replications, namely PBS 0.1 mL/fish, A. hydrophilla 106 CFU/ml as much as 0.1 mL/fish, Enterococcus sp. 106 CFU/ml as much as 0.1 mL/fish, Lactobacillus sp. 106 CFU/ml as much as 0.1 mL/fish and Lactococcus sp. 106 CFU/ml as much as 0.1 mL/fish. Treatment of the bacteria to fish were used intra muscular injection. Clinical symptoms, anatomical pathology and hematological were analyzed descriptively, while survival was analyzed statistically using ANOVA. Tilapia with an average weight of 30 g/fish were reared for 7 days in an aquarium measuring 50Ã—40Ã—30 cm3 with a volume of 40 L of water, at a density of 10 fish/aquarium. Fish are fed 3 times a day. Administration of lactic acid bacteria Lactobacillus sp. showed the highest survival rate compared to the treatment with other isolates. Hematological were in the normal range in all treatments except for the treatment with A. hydrophila bacteria. The three lactic acid bacteria from the intestines of repang fish are safe for tilapia and can be used in further probiotic tests.Â Â Keywords: Lactid acid bacteria, pathogenicity, A. hydrophila, hematology

Co-Authors , Agustina Agus Sabdono Agustina Agustina Agustina Agustina Agustina Agustina Aipassa, Marlon Ivanhoe Andi Noor Asikin Andre Jolpano Catur A Pebrianto Eko Budi Prasetyo Esti H Hardi Esti Handayani Hardi Feby Kristina Fikri Ardhani Ida Ayu Putu Sri Widnyani Irawan Wijaya Kusuma, Irawan Wijaya Kiswanto Kiswanto Nurkadina Nurkadina Puput Andriani Rahmi Nohon Salaho Dina Devy Slamet Budi Prayitno Sundari Hidayat Sutrisno Anggoro Suwinarti, Wiwin Taufan Purwokusumaning Daru, Taufan Purwokusumaning Widyaningsih Rahayu Yosep Ruslim

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